Asahipak - Hplc.eu

Maltooligosuccharides, organic acidsand ethanol12 34567Sample :0.05% each, 20μL1. Maltotetraose2. Maltotriose3. Maltose4. Glucose5. Lactic acid6. Glycerol7. Acetic acid8. Methanol9. EthanolCello-oligosaccharides and furfurals1 2 34567Sample : 0.1% each, 10μL1. Cellopentaose2. Cellotetraose3. Cellotriose4. Cellobiose5. Glucose6. Glyceric acid7. Acetic acid8. Furfural9. 5-HMF8Standard organic acidsSample :1. Citric acid2. Tartaric acid3. Pyruvic acid4. Malic acid5. Succinic acid6. Glycolic acid7. Lactic acid8. Fumaric acid9. Acetic acid10. Levulinic acid11. Pyroglutamic acid12. Propionic acid13. Isobutyric acid14. n-Butyric acid2 4135679810111213 14Standard analysiscolumns9980 5 10 15 20 25 0 5 10 15 20 25 30 35 40 4510min min min20 30Column : Shodex SUGAR SH1821Eluent : 0.5mM H 2 SO 4 aq.Flow rate : 0.6mL/minDetector : RIColumn temp. : 75˚CHighly hydrophobic organic acids12 3Eluent = 2mM HClO 4 aq.42052567830 35 40 minEluent = 2mM HClO4 aq./CH3CN=90/10Sample :1. Succinic2. Lactic3. Formic4. Acetic5. Propionic6. Isobutyric7. n-Butyric8. Isovaleric9. n-ValericTo shorten analysis time9Column : Shodex SUGAR SH1821Eluent : 2mM H 2 SO 4 aq.Flow rate : 0.6mL/minDetector : RIColumn temp. : 60˚COrganic acids and vitamin C in fruit juice21345 101520 min56Sample :1. Cl - ,etc.2. Oxalic acid3. Maleic acid4. Citric acid5. Malic acid6. Vitamin C7. Succinic acid8. Formic acid9. Acetic acid789Organic acids in black vinegarUV(210nm)RIColumn : Shodex RSpak KC-811 x 2Eluent : 6mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 50˚C1122Sample : Black vinegar, 20μL1. Gluconic acid2. Lactic acid3. Acetic acid4. Pyroglutamic acid334437Columns for Ion Exclusion Chromatography15202530 min510 15 20 25 30minColumn : Shodex RSpak KC-LG + KC-811 x 2Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 47˚CColumn : Shodex RSpak KC-LG + KC-811 x 2Eluent : 1mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 40˚CColumn : Shodex RSpak KC-811x 2Eluent : 3mM HClO 4 aq.Flow rate : 1.0mL/minDetector : UV(210nm), RIColumn temp. : 50˚COrganic acids in Japanese sakeCyan and cyanogen chloride withpost column methodSample : 100μL1. Phosphoric acid etc.2. Citric acid3. Pyruvic acid4. Malic acid5. Succinic acid6. Lactic acid7. Fumaric acid8. Acetic acid9. Pyroglutamic acid56Fine rice wine56Fermented liquorPure rice wine546CN -Sample : 10μg/L each, 100μLCNCl14114238792378 9238792 4 6 8 10 min 2 4 6 8 10 min0 5 10 15 20 25min0 5 10 15 20minColumn : Shodex RSpak KC-LG + KC-811 x 2Eluent : 4.8mM HClO 4 aq.Flow rate : 1.0mL/minDetector : VIS(430nm)post column methodColumn temp. : 63˚C25 0 5 10 15 20 25minColumnEluentReagent AReagent BFlow rate: Shodex RSpak KC-811 6E: 1.0mM H 2 SO 4 aq.: Chloramine T solution: 4-Pyridinecarboxylic acid-Pyrazolonesolution: (Eluent) 1.0mL/min(Reagent) 0.5mL/min each: VIS(638nm)DetectorColumn temp. : 40˚CReaction temp. : (Reagent A) 40˚C(Reagent B) 80˚C