Asahipak - Hplc.eu

Calibration curves for GS-HQ series usingpullulan and PEGMolecular weight10 510 410 310 2 4 5 6 7 8 9 10 11 1210 6 Elution volume (mL)GS-320 HQGS-620 HQGS-520 HQGS-220 HQPullulanPEGPeptidesGS-HQ columns can be used not only for SEC (GFC)in an aqueous system, but also for multimodalanalysis where hydrophobic interaction and ionicinteraction are used together as separation criteria,under certain conditions of the eluent. This resultsin unprecedented separation analysis.GS-HQ columns are excellent in the performancefor separating hydrophilic peptides, in particular,acidic or basic peptides, from each other.Sample :No.123456Val-Glu-Glu-Ala-GluGlu-Ala-GluVal-Glu-Ser-GluArg-AspGly-His-LysArg-Pro-Lys-ProMW576347390289340497Σf1.780.390.830.680.293.24pl3.023.123.126.759.9511.44Molecular weight10 3GFC132410 2 21 36556Standard analysiscolumnsΣf : Hydrophobic parameterpl : Isoelectric point4Column : Shodex Asahipak GS-HQ seriesEluent : H 2 OFlow rate : 0.6mL/minDetector : RIColumn temp. : 30˚CLow molecular weight, water-soluble dietary fiberMonosaccharides, disaccharides,and sugar alcohols are eluted withretention times larger than thatindicated by the arrow. Thisfacilitates the separation of thosesubstances from indigestiblefractions, making thedetermination of small,water-soluble dietary fiber moreefficient.Column : Shodex Asahipak GS-320 HQEluent : 50mM Ammonium acetate buffer(pH7.0)Flow rate : 0.5mL/minDetector : UV(220nm)Column temp. : 30˚CAnalysis of the purine base in beersguanine10 15 20 25 minPurine bodies in food are analyzed after being neutralized to a purine base by hydrolyzing thehomogenized and freeze-dried food with a 70% perchloric acid. This section presents an example ofanalysis of purine base in ordinary beer and purine base in beer treated with guanase (an enzyme thatdegrades guanine to xanthine). The following data indicates that guanine was decreased and xanthinewas increased by guanase.Normal beerGuanase treated beer49Multimode columnsxanthinexanthine1015 20 25 30 35 40 45 50min010 20 30 40 50 min 0 10 20 30 40 50 minColumn : Shodex Asahipak GS-220 HQ x 2Eluent : H 2 OFlow rate : 0.5mL/minDetector : RIColumn temp. : 60˚CColumn : Shodex Asahipak GS-320 HQEluent : 150mM Sodium phosphate buffer(pH2.5)Flow rate : 0.6mL/minDetector : UV(260nm)Column temp. : 35˚CData provided by Prof. Kiyoko Kaneko,Faculty of Pharmaceutical Sciences,Teikyo University“Umami”A multimode column GS-320 7E supports an easy andsimultaneous analysis of major nucleic acids that arepresent in taste components, i.e., IMP, GMP, andAMP, and derivatives thereof with isocratic elution.Sample :1. IMP2. GMP3. AMP4. Inosine5. Hypoxanthine6. Guanosine7. Guanine8. Adenosine9. Adenine321456789Gelatin analysis with PAGI methodSample : 100μLGelatin(Koepff 16922), 0.2%High sensitivity analysis of metal-bindingamino acidsSample : 100nL1. γ-Glutamylmethylselenocysteine2. MethylselenocysteineCOOH2H12 NCOOHHNCOOHOSeCH 3g-glutamyl-Se-methylselenocysteine(gGluMeSeCys)SeCH 3H 2 NSe-methylselenocysteine(MeSeCys)5 10 15 20 25 30 35 40 0 5min10 15 20 25 30Retention time (min)0 102030 minColumn : Shodex Asahipak GS-320 7EEluent : 10mM Sodium phosphate buffer(pH5.0)Flow rate : 1.0mL/minDetector : UV(260nm)Column temp. : 30˚CColumn : Shodex Asahipak GS-620 7G-P x 2Eluent : 0.1M KH 2PO 4 aq./0.1M Na 2HPO 4 aq.=50/50Flow rate : 1.0mL/minDetector : UV(230nm)Column temp. : 50˚CColumn : Shodex GS320A-M5DEluent : 50mM CH 3COONH 4 buffer(pH6.5)Flow rate : 2.0μL/minDetector : ICP-MS(Se at m/z=82)Data provided by Yasumitsu Ogra,Professor of Showa Pharmaceutical University