Analysis of Genes for Stigma Coloration in Rice - IRRI books

Iwata, Kyushu University: F1 60, F1 85, F1 204, F1 214, F1 215, F1 217, F1 222, F1
232, F1 233, F1 240, F1 244, F1 260, F1 283, F1 288, F1 293, F1 297, F1 304, F1 310,
F1 312, and F5 814. Tongil-type Milyang 77ms was crossed with 10 Tongil-type
marker lines (Suh and Heu 1978): 1001, 1003, 1005, 1006, 1007, 1009, 1010, 2021,
1023, and 1036, and with IR42667-36-mpl having the mp-1 gene. Linkage relationships
were investigated from the F 2 s. Recombination values were calculated by the
minimum discrepancy method (Murty 1954).
Male sterility of Milyang 67ms
The linkage relationships between the GMS gene of Milyang 67ms and the marker
genes belonging to linkage group III are shown in Table 1. Of 22 marker genes (linkage
group given in parentheses)— C (I), 1g (II), nal-1 (II), eg (III), lax (III), d-10 (III), A (III),
d-18 (III), fs-2 (III), spl-5 (IV), rfs (IV), spl-7 (VI+IX), nl-1 (VI+IX), la (VIII), d-k-4 (X),
ch-1 (XI), ch-2 (XI), ch-3 (XI), fc-1 (XI), dl (XI), fl (fgl), and rk-2 (fgl)— eg, lax, d-10,
and A belonging to linkage group III, from marker lines F1 204 and F1 297, appeared
to be linked with ms-m67(t). The other markers tested were independent of ms-m67(t).
Complete linkage was found between ms-m67(t) and the lax (lax panicle) gene in the
repulsion phase. Relatively close linkages were found between ms-m67(t) and eg (extra
glume), d-10 (dwarf-10), and A (anthocyanin activator), with recombination values of
13.7, 23.6, and 34.0%, respectively. Marker genes d-18 (dwarf-18) and fs-2 (fine
stripe-2) of linkage group III were independent of ms-m67(t). Recombination values
between eg and d-10, d-10 and A, and eg and A were calculated as 31.2, 47.2, and
50.5%, respectively. From these results, we can assume that ms-m67(t) is located on
linkage group III in the sequence eg-ms-m67(t) - d-10 - A, and is located distantly from
d-18 and fs-2 (Fig. 1).
Table 1. Linkage relationships between the male sterility gene ms-m67(t) of japonica male
sterile rice Milyang 67ms and marker genes eg (extra glume), lax (lax panicle), d-10 (dwarf-
10), A (anthocyanin activator), d-78 (dwarf-18), and fs-2 (fine stripe-2) belonging to linkage
group III.
Genes combined Linkage F 2 phenotype
phase
A B
AB Ab aB ab
Total
c 2a
Recombination
value (%)
ms-m67(t)
ms-m67(t)
ms-m67(t)
ms-m67(t)
ms-m67(t)
ms-m67(t)
d-10
d-10
eg
eg
lax
d-10
A
d-18
fs-2
eg
A
A
Repulsion
Repulsion
Repulsion
Coupling
–
–
Coupling
Coupling
Coupling
449
389
455
402
214
214
568
347
352
211 216 4
204 208 0
205 208 12
258 93 127
66 68 19
66 71 16
115 117 100
316 148 69
313 143 72
880
801
880
880
367
367
880
880
880
80.15**
100.00**
57.75**
23.29**
1.07
2.54
76.70**
16.62**
12.14**
13.7
0.0
23.6
34.0
Independent
Independent
31.2
47.2
50.5
a ** = significant at the 1% level.
122 Suh et al