Olympus BHS/BHT System Microscopes (BH-2) brochure 1987

System Microscopes

BH2 Series System Microscope
The BH2 Series of topquality microscopes facilitates the use of a wide range of microscopic
techniques, depending on the choice of accessories. The Koehler-type illumination elicits
the full performance of the LB Series objectives, yielding photomicrographs of the highest
quality. This series of microscopes is suitable for a very wide range of applications, from
routine laboratory work to educational and research investigations.

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The design of the entire system--microscope
stands, observation tubes, stages, etc.-bears
witness to creative and inventive thinking.
One example may be seen in the design
of the coarse stage adjustment, in which
movement is limited in the upward direction
in order to prevent contact between objective
and specimen. The coarse and fine focusing
knobs are of coaxial design, and the mini-
mum increment on the fine adjustment knob
represents a stage mwernent of just two
microns. The coaxial stage control knobs
are located close to the focusing knobs,
low enough to permit operation witt~wt
having to raise the arm off the arm rest.
In addition, the BH2-TR30 trinocular observa-
tion tube features constant tube length
adjustment which allows camera focus
through the binocular tube in phatamicrb
graphy, thereby adding significantly to
ease of operation.
Improved Illumlnatlng System
All BH2 Series microscopes employ the
widely accepted LB Series objectives and
WHKl OX eyepieces (as standard equipment).
providing F.N. 20 widefield observation.
BH2 Series microscopes provide Koehler
illumination to exploit the full benefits of the
LB Series objectives. A wide choice of
condensers provides uniform illumina:icm
from ultra low to high magnifications.
These microscopes incorporate precentered
halogen lamps, aspherical collectors and
fully enclosed light paths designed to keep
out dirt and dust.

BHS System Microscope
This top quality microscope features a long-life 12V, I# W halogen lamp which provides
bright illumination for a wide range of applications. It is ideally suited for advanced research
work and investigatians requiring high precision.

BHSF Mhwcqm Stand
.The BHS-F stand is of a robust and stable
design unaffected by vibration or shaking.
Highly reliable, it is ideal for photomicro
graphic purposes.
.The stand incorporates a highperfom
transformer which is unaffected by fluctuations
in the line vdtage and so always
provides light of uniform brightness and
mlor temperature.
.The ball bearing sextuple revohring nose
piece accepts law to high magnification
objectives with 45mm parfocal distance.
This feature contributes significantly to
greater efticiency in microscopy.
BHSLSH Halogen Lamp Housing
The IN l W halogen lamp provides a
bright light source suitable for many types
of microscopy. The precentered lamp has
an average life of around 2,000 hours,
reducing slgnlflcantly the time lml with
troublesome bulb change.

BHT System Microscope
Employing the same revoking nosepieces, stages, observation tubes, etc., as the Model
BHS, the Model BHT microscope uses a different type of illumination system and electrical
components. A compact microscope, it offers excellent cost-performance.

BHFF Mkmscmp Stand
*Smaller than the BHS model, with a
different transformer arKl lighl source.
Extremely functional.
*The dust proof illuminating system keeps
the light path clean.
LS#HI Lamp HWng
Encloses a precentersd 6V 20W halogen
t .

LB (Long Barrel) Objectives
The LB (Long Barrel) Series ranges from inexpensive objectives to top quality and special-purpose
objectives. Thls extensive selection enables the user to choose the ideal lens for the
purpose intended.
In the design of LB objectives, OLYMPUS
has drawn upon its extensive and renowned
expertise In optical technology. The objectives
offer the following features:
1. High resolution
2. Superior contrast
3. Excellent field flatness
4. F.N. 20 widefield observation (with
WHKl OX eyepiece)
5. Ultra-!ow magnification objectives are
parfocal wtth high-magnification lenses.
S Plan Apodumat Objactlm
These objectives are of the highest quality
design. Chromatic aberration is thoroughly
compensated, field flatness is superb and the
high numerical aperture provides outstanding
resolution.
S Plan Aohmmat ObJechs
The S Plan objectives are the most popular
high quality objectives in the LB series.
Excellent optical correction across the
entire lens surfaces makes them ideally
suited for use in differential interference
contrast microscopy and for super widefield
observation.
S Plan FL ObJacthres
The ultra low magnifications (lX, 2X) of these
objectives make them ideal far examinatkm
of large specimen areas. They are fully
parfocal with other LB cbjetiis. The
SRanFL2X objective is suitable for super
widefieid otservatlons.
Note: The objectives prform bast with the BHPULC
ultra-low condenser.
D Plan Achromat ObJedw
With D Plan objectives, fleld flatness is
guaranteed up to F.N. 20. Ideal for photo
micrographic purpses, they are widely used
in research, educational and routlne work.
D mrolmt Objective8
These are the most economically priced
objectives in the LB series. Resolution and
flatness in the center portion of the field
of view are excellent and they are particularly
suitable for routine work, educational
purposes or training.
N o ~ O b ~
This type of abjective is ideal for examination
of specimens with no cover glass, such
as blood smears.
'A varlety 01 other types of objectim are available for
dlffarent applicatOwts and requirements, including
phmtrast, reflected light flwrescmce, or
polarked light microscopy.
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Eyepieces and Condensers
To complement the performance of our LB objectives, an extensive range of eyepieces and
six types of condensers are available. To exploit the full performance of objectives with
magnifications from IX to lOOX requires the choice of the proper condenser.
WHKlNK Eyepieces I h
LB Serbs Eyepieces
Designed for use with LB Series objectives,
WHK eyepieces are easy and comfortable
to use and are of widefield Win (F.N. 20
with the WHKlOX eyepiece). The high eye
point makes them easy to use by persons
who wear glasses and enables fatiguefree
observation over long periods of time.
Other eyepieces in the series include the
WHK8X, WHK15X, NK5X and NK20X.
NFK Serks Photo Eyapkcss
These photo eyepieces are speclflcally
designed for photomicrography. They are
available in 2.5X, 3.3X, 5X and 6.7X
magnifications.
condemm
An extensive range of condensers is available,
covering brightfield and darkfield observations.
The BH2-AAC is a top quality achromatic
aplanatic brightfield condenser which permits
full compensation for all aberrations. The
swingat BH2-SC condenser is suitable for
a wide range of magnifications. Several other
condensers are available, including the
general-purpose BH2CD and the BH2-ULC
for ultra-low magnification.

Observation Tubes
All BH2 series observation tubes have constant tube length adjustment. This feature
automatically maintains the optimum observation conditions when interpupillary distance is
varied, without requiring troublesome adjustments.
BHBB130 Binocular Obswatlon fibe
The BH2-6130 is designed exclusively for
visual observation.
Inclination: 30' to the horizontal
lnterpupillary distance: 53-75mm (with
constant tube length adjustment)
Maximum F.N.: 21
BHSTR30 Mrmcular Observation Tube
This tube increases the efficiency of photo
graphic work since it perrnRs camera focusing
through the bkocular section. The three-step
llgM path selector allows photwnicrography
and observation at the same time.
Inclination: 30" to the horizontal
lnterpupillary distance: 53-75mm (with
constant tube length adjustment)
Maximum F.N.: 21
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BHNW Sup.r Wkkfleld Attachment
The BH2SW attachment presents the observer
with a field of view about twice the size
of that obtained with wdinarv obwrvatbn
tubes, and thus reduces to a minimum the
need to move the specimen around. This I
resutts in a sinnificant Increase in work 1
efficiency. Ths 3-step light path selector
and constant tube length adjustment allow I
camera focusing through the binocular
section for photomicrography. I
Inclination: 30' to the horizontal
Interpupillary distance: 56-75mm (with
constant tube length adjustment)
Maximum F.N.: 26.5
BH2.m Wkal Photohrbe
This tube presents the most economical way
of conducting photomicrography. The bim
cular observation tube must be removed in
wder to attach the vertical phototube and so
ohenration is not pssible at the same
time as photomicrography.

Stages
A selection of stages is available, including those suitable for differential interference contrast
and fluorescence microscopy.
Specimen Holders
BH2SVR Mechankal Stage with
RlgM-Hand Low Drfve Contrds
This is the mast popular stage for many
areas of research and testing. Covered ball
races prevent contamination by dust and glass
chips. Scanning area is 76mm O() x 50mm 0.
A 270" rotation capability makes this stage
highly suitabie for photographic framing and
for differential interference contrast purposes.
Note: Range of rotation is reduced to 65' when a Met-
ential interferencecontrast condenser is attached.
BH2WL Mechanical Stege with
Left-Hand Low Olhre Controls
Has the same features as the BH2-SVR,
except that the controls are located on the left.
BH2SH Mechanical Stage wlth
Horhontal [Mvo Contds
The stage controls are horizontally located
on the left- and right-hand sides. The stage
can be rotated through 40' when the stage
control knobs are on the far skJe, and
through 180° when on the near side.
BH2SRG Circular Rotatable Stage
This is the ideal stage for Nomarski differential
interference contrast and polarizdlight micro-
scopy. It features a centering mechanism,
and graduations at 1 " intervals around the
entire circumference. Stage diameter is
142mm. It accepts stage clips or KM and
FM attachable mechanical stages.
BH2SVFR Grooved Meshanlcl~l
Stage with
Right-Hand Law hfve Controls
The fluted design prevents slides sticking
to the stage surface due to spilled immersion
oil, thus eliminating interruptions In micrs
scopic work. The X-axis excursion is 52rnm,
and Y-axis excursion is 76mm. The stage
rotates through 270'.

BH2-PC Phase Contrast Attachment
BH2-KPC Simple Phase Contrast Attachment
Phasecontrast is one of the most commonly used techniques in microscopy. It is employed
in the examination of living micro-organisms, cells, etc., permlttlng observation of the
internal structures of such specimens under differen f degrees of contrast.
BWPC Pham Contnst Attachment
*Features an Abbetype turret condenser
wtth a numerical aperture of 1.25.
Incorporates annular phase plate for lox,
20X, 40X and 100X phase objectives.
Incorporates an aperture diaphragm for use
in brighMeld. This diaphragm Is automatically
disengaged in phasecontrast observation.
*The CT-5 centering telescope with hQh eye
point permits slmple and accurate annulus
alignment.
*The BH2SW super widefield attachment
used in conjunction with S Plan phase-
contrast objectives enables observation of
super widefield phase contrast images.
*Four types of mtrast-PL, PLL, NH and
NM-are available.
Two types of objectives are available for
phasecontrast microscopy: S Plan Achre
mats and D Achromats. They come in
magnifications of 10X 2OX, 40X and
100% and in bth positive and negative
contrast.
BH2-KPC Slmple Phese Contnst
Attachment
Used in conjunction with a BH2CD brightfield
condenser and phase contrast objectives,
this attachment presents phase contrast
images easily and economically. Available
in 1OX 20X and 40X magnifications. I
PL = POSI~VE
LOW NH = NEGATIVE HIGH
PU= POSITIVE LOW-LOW NM =NEGATIVE MEDIUM

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Green Filter
Phase annular ring
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Module
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BH2-PC1 ORS
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BH2-N IC Nomarski Differential
Interference Contrast Attachment
The Nomarski differential interference technique is used for unstained specimens, such as
living cells. Intercellular structure end surface variations can be obsenred as a three
dimensional image under various interference colors.
*The turret condenser (NA 1.40) is fitted
with Nomarskl brisms for lox, 20X, 40X and
lOOX magnifications, as well as light annuli
for 1 OX and 40X phase contrast microscopy.
These features enabte cornparisms
bemen phase contrast and DIC images
with the greatest of ease.
aSiandard S Plan Achmt objecth can
be used for DIC microscopy. Super
widefield DIC observation is also possible.
.Has an opem aperture for briihtfield
chewation.
.The Internate attachment has s magnifi-
cation factof of 1 .a.
*IF550 interference filter uGhmv

15
BH2-RFL Reflected Light Fluorescence Attachment
The development of new optics makes it possible to use high-energy exciting light,
providing brighter fluorescence and improved results.
.Three levels of exciting light intensity
are available: 100%, 25% and 0%.
.An aperture diaphragm allaws ttle precise
regulation of exciting light intensity and
fluor- image contrast.
*Features a built-in field diaphragm with
centering mechanism. This feature eliminates
stray llgM which caw flares and
reduces image clarity.
*The synchronized exciting filters, dichroic
mirrors and barrier fitters all move together,
allowing swttch over between two wave
h g b at a single touch. F i types of
exciting filter sliders are available, each
Wing two exciting fitters. Exciting filters
come in five combinatians: U-V, B-G, U-B,
Bu and UG.
*Additional exciting filters and barrier filters
W Y be employed.
mFluorescence attachments using a halogen
ligM arce are available for use with FlTC
staind s~ecirnens which can be examined
with a retatlvely small amount of exciting
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light. Both reflected light and transmitted
light types are available.
Btl2-RFLB
A reflected lit fluorescence attachment
with halogen tight source, designed for B
excitetian.
BH2-FITW
These hNo transrnittdQht fluorescence
units are designed specifically for B
excitation and emplay the lmtt halogen
lamp in BHS microscope as their light
source. The secondary transmission region
at about 630n m in these FtTC filters enables
the observation of non-fluorescence areas
under red &Meld illumination.
*D Plan Apo UV Series objectives
feature an apochromatic design to correct
b
chr~maticabermtkrtscwerawidewavelerigth
range. This design guarantees a sharp
knage and brlgM Inmination in any excitatian.
The new, flwrescencefree imrsbn oil
is also suitable for other dl Immersion
~usedforgeneral~tion.
~alsooffersallneupof4typesof
fluorescent phase contrast objectives
(D Plan Apo/D Apo UVPL Series).
When a flwrescent specimen is being
scanned, fading of fluorescent light can be
pwnted by switoh'hg to phase mtrast
~.Whenrellectedl'~fiuorescence
and transmitted IIgM phase contrast
&mmtim are performed simultanewsly,
an entire specimen Image, induding the
non-fluorescent area, can be obtained.
D Plan ApolD Apo UV Series D Plan ApolD Apo WPL Series -

iH2-RFL Standard OU
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BH2-FITC Standard Ouffits
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Excitation FlTC Interference Filter Set
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(Transmitted light)

Attachments for Group Observation
Here is a group of very useful accessories which allow simultaneous viewing by several
observers. This is a parficularly convenient feature for discussions between researchers,
and is indispensable for teaching purposes.
BH2-MDO MUHI-viewing Attachment
.Permits simultaneous observation of the
same specimen image in the same orienta-
tion by up to five persons.
*The arrow painter can be moved to indicate
any area of the specimen. Pointer color
(green or orange) can be changed, and
brightness adjusted.
Standard Outfits
BH2-00 Dual-viewlng Attachment
.The features are exactly the same as the
BH2-MDO, except that tl permits observation
by two persons.
*There is ample distance between the
oberver posklons, allowing relaxed viewing.
*The second observer may manipulate the
pointer by means of an additional lever
located on the opposite side from the
main lever.
Standard Outfits
is used on a BHS System mkrmeape.

AHSPSW Hlgh Resdutlon
Projectlcm Semen
Eye strain is minimal even after long periods
of viewing thanks to the high resolution
presented by this screen.
Effective diameter: 155mm
Screen image magnification: Objective
magnification x NFK photo eyeDiece
magnification x 3
AH-MPSW Pmjecth Semen
The AH-MPS-W is extremely useful in small
group viewing and discussions.
Effective diameter: 155mm
Screen image magnification: Objective
magnification x NFK photo eyepiece
magnification x 3
BH-WP Wall Projdon Prkm
Used to project an image onto a wall
or screen.

PM-IOADSIPM-10AD Photomicrographic and
Cinemicrographic Systems
These fully automatic Photomicrographic systems are designed for exclusive use with microscopes.
A built-in microcomputer assures easy and failurefree operation. They can also be operated
manually, and both accept large format film.
PM-1OAbS Autamatk Pbtmicrographk
SY-m
.Features both spot (1 %) and Integrated
(30%) metering.
mThe built-In mlcrocwnputer autamatically
cwnptwates for a film's recipmity failure
characteristics and cmtrols expmre time
correctly even during lmg exposures.
*An exposure adjustment feature allows the
precise control of expmre tlme to match
the spclmen's characteristics.
*Incorporates an AE lock mechanism,
useful when trimmlng with spot metering
or to keep density unlform in wntlnuws
pmorarnic photography.
Manual exposure control is also available.
.Accepts the following types of film: 3Smm;
4" x 5" sheet; and 3'k" x 4lk" Polaroid.
*The multiple exposure mechanism enables
a number of images to be recorded on the
same frame of film.
PM-IOAD Aul~metlc Photoml~r~grephk
w-n
Has the same features as the PM-1MS
except that it provides only 60% metering
with 35mm film.
Note: This system accepts practically dl the accas-
sorb avellable for the PM-tOADS.
PMCTR Cobr Tompatun Modub
The PMCTR measures color temperatures
from 2,500K to 10,000K. It provides greater
accuracy In color temperature readings.
PM-IV Ttms L ap Control Unlt
Used in conjunction with the PM-1OADS or
PM-I OAD, this unit automatically controls
the intervals in timelapee photography.
Intervals can be set f m one second to
99.9 hours. Permits a total of 9999 frames
to be taken.
PM-VSB BrlgMhame Mlnrkr
Thls viewNnder Is optionally available for the
Olympus Photomicrographic Systems
PM-1OADS and PM-1 W.
Focusing on the double crosslines and the film
format frames when photographing dark
specimens against a dark background is
greatly facilitated by the fact that the frame
reticle is illuminated and the color of the
frame can be varied from black to red or yellm.

BHSKP Sknple PolaWng Attachment
This attachment enables polarized-light
microscopy using ordinary LB objectives.
A tint plate and other compensators can
be used with it.
Standard Outffts
BH*POL Palarfdn~ Flht Set
Consisting of a palarlzer and an analyzer,
this accessory is usefull for detecting
birefringent specimen details.
BHMA MagnHkatkn Changer
Magnification can be set at three levels:
1 X, 1.2% and 1.5X. The phasecontrast annuli
can be aligned with the bullt-In Bertrand lens.
BH2-DA Drawing Attachment
Very useful in the accurate sketching of a
magnified specimen image seen through the
microscope. Adjustable magnficatbn.
BBFH =gout Fllter Hdckr
Holds three filters at one time. Rtters can
be exchanged at the touch of a finger,
resulting in more efficient observation and
photomicrography.
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1. Candidiosls (UuOdenurn, Grooott stain). SPlanP(, NFK2.5XLD
2 Alkaline phosphatase (liver). SPlanlOX, NFK3.3XL.D
3. Acld phosphatase (spleen of Gaucher's disease). SPlanlOX, NFK3.3XLD
4. Nlcotin amkie adenine dinucleatide phosphate dehyclrqlmase (cell line derived
from human malignant melanoma-SEKI). SPlen40X, NFK3.3XL.D

liaaram of the BHS. BHT and PM-IOAD. PM-IOADS

Svstem

25
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L R nhjective Series for Biological Use
S Plan Fluorite Obiedvea
D Plan Achromatic Objectives
D Achromatic Objectives
Phase Contrast Objectives
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D.1.C. = &8lskl DIff~liltIth! hiWfBliMC9 c0ntf8~t.

UM3.3XLD -
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LB Eyepiece Series
to Eyepieces

It takes a tremendous amount of skills to build a reputation
as an innovator among industries as diverse as
communications, medicine, information and science
Yet that's exactly what Olympus has accomplished
since its inception in 1919.
Our varied product list is filled with technological
achievements and resounding successes.
Not only in cameras, but also in a wide range of Microscopes.
Fiberscopes. Microcassette recorders.
Clinical analysis equipment. Video equipment
And more breakthroughs are on the way,
particularly in the exciting new field of optt~lectronics,
which combines the resources of optics,
electronics and precision engineering.
At Olympus, we've earned our reputation
with an unfailing commitment to heavy research and
development With an uncompromising dedication to quality,
precision and accuracy.
And with a stubborn unwillingness to follow the crowd.
That's why we'll continue to lead the way
with original products that surprise you,
assist you, involve you, and fulfill you.
1 Photographic.
Medical,
Microscopic,
Industrial & Business Equipment
4 Newdp O W. l& SUCcB88. N.Y. 11042.1 179. USA
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