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Assessment of degree of bacterial colonisation of ... - Addemar

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Pseudomonas aeruginosa<br />

Total loss <strong>of</strong> viable cells was noted at day 3 in cells suspended in Ringer’s solution<br />

and by day 5 in cells suspended in media. A slower drop in cell numbers occurred in<br />

bacteria in media, compared to bacteria in Ringer’s solution (Fig. 7).<br />

8<br />

log 10<br />

cfu/ml<br />

7<br />

6<br />

5<br />

4<br />

3<br />

Ringer's solution<br />

Media<br />

2<br />

1<br />

0<br />

Day 0 Day1 Day3 Day5<br />

Figure 7. Cell concentration <strong>of</strong> Pseudomonas aeruginosa added to coated discs over<br />

time. Error bars indicate standard deviation.<br />

Negative controls<br />

No <strong>bacterial</strong> growth was seen on any negative controls used in this experiment.

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