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Assessment of degree of bacterial colonisation of ... - Addemar

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Listeria monocytogenes<br />

Complete loss <strong>of</strong> cells in Ringer’s solution was noted by day 3, and by Day 5 in<br />

media. The drop in cell numbers was much more rapid in bacteria suspended in<br />

Ringer’s solution, falling to 2.5x10 3 cfu/ml by day 1. This compares to a <strong>bacterial</strong><br />

count <strong>of</strong> 7.9x10 4 cfu/ml in cells suspended in growth media (Fig. 3).<br />

8<br />

7<br />

log 10 cfu/ml<br />

6<br />

5<br />

4<br />

3<br />

Ringer's solution<br />

Media<br />

2<br />

1<br />

0<br />

Day 0 Day1 Day3 Day5<br />

Figure 3. Cell concentration <strong>of</strong> Listeria monocytogenes added to coated discs over<br />

time. Error bars indicate standard deviation.

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