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Petri Dish Inserts - Bioscience Tools

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Oocyte<br />

Chambers<br />

Call 1-877-853-9755<br />

Small Working Volume<br />

Perfusion Chambers<br />

Easy to use<br />

Fast perfusion<br />

Build-in suction - washout tubing<br />

Mount an any surface<br />

Compatible with perfusion systems<br />

Glass bottom for imaging<br />

Oocyte Perfusion Chamber<br />

Expression of recombinant receptors in oocytes have been one of the favorite choices for researchers<br />

to assess the pharmacology and physiology of receptors. Since the oocytes are 1 - 1.5 mm in diameter,<br />

however, the fast solution exchange around the oocyte can be a problem. After several years of electro-<br />

physiological research, we have designed a Xenopus oocyte perfusion chamber for use in automated and<br />

unattended experiments. One of the major characteristic of the chamber presented is a small working<br />

volume (~10 μl), which allows faster solution exchanges. Combined with perfusion systems, this cham-<br />

ber lets you to obtained dose-response data fast and easily. It can be used with Oocyte Voltage Clamp<br />

amplifiers, which employ one or two electrodes bath clamp. Fits perfusion manifolds. The suction tubing<br />

requires connection to a vacuum source. If you do not have in house vacuum, we recommend an outflow<br />

unit for suction to remove solution during perfusion. Improved design makes using the chamber easier: the<br />

replaceable chamber is fixed inside a microscope stage adapter using flat springs. Improved profile of the<br />

suction tubing provides smooth solution flow. Comes with Allen wrench.<br />

• Dimensions: mm x 3mm<br />

• Media Exchange: fits zero-dead volume<br />

manifold ZMO, faster solution application<br />

• Working volume: approx. 0 microl<br />

63

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