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Prohibited Ingredients - TTB

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<strong>Prohibited</strong> <strong>Ingredients</strong> (Continued)<br />

• Stevioside and Rebaudioside A:<br />

– Found in extract of stevia rebaudiana<br />

– Sweetener often used in alcoholic beverages<br />

such as soju<br />

– Stevioside is prohibited for use in food or beverages, highly<br />

purified reb A recently approved by FDA<br />

– Developed an HPLC/DAD method for the detection of<br />

stevioside<br />

• Aminopropyl column, 250 mm x 4 mm, 5 um particle size<br />

• Acetonitrile/water mobile phase – isocratic<br />

• 205 nm

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