Prohibited Ingredients - TTB

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• Pesticides: Pesticides in Wine (Continued) – Agilent 6890 GC equipped w/5973A mass selective detector and automatic liquid sampler – HP-5ms cap column: 0.25mm, 30m, 0.25um, constant pressure mode using retention time locking – SIM mode using three programs • Organohalogen • Organonitrogen • Organophosphorus – QC samples • 50 ppb standard • Water blanks and spikes • Wine blanks and spikes
Extracted Ion Chromatogram -Dimethomorph I and II Abundance Response 5500 5000 Ion Exp% Act% 301 100 100 Response 4500 303 35.20 34.51 Ion Exp% Act% 4000 3500 3000 2500 2000 1500 165 41.40 41.72 387 26.50 25.27 Ion 301 Ion 165 Ion 303 Ion 387 301 100 100 303 34.90 34.52 165 42.90 44.06 387 25.00 25.31 1000 500 Time--> 0 36.00 36.20 36.40 36.60 36.80 37.00 37.20 37.40 37.60 37.80 38.00 38.20 38.40
Page 1 and 2: 2009 TTB Expo Presentation Laborato
Page 3 and 4: Background • On Jan 24 2003, Home
Page 5 and 6: TTB Duties • Responsible for ensu
Page 7 and 8: Prohibited Ingredients
Page 9 and 10: Prohibited Ingredients (Continued)
Page 19 and 20: Limited Ingredients • Typically,
Page 21 and 22: Limited Ingredients (Continued) •
Page 27: Pesticides in Wine • Analysis of
Page 31 and 32: Ethyl Carbamate • Presence of EC
Page 33 and 34: Ethyl Carbamate (Continued) Abundan
Page 35 and 36: Food Allergens Background and Regul
Page 37 and 38: • Immunological: Food Allergy/Int
Page 39 and 40: Food Allergens and Regulations •
Page 41 and 42: Comments from ANPRM (Continued) •
Page 43 and 44: Comments from ANPRM (Continued) •
Page 45 and 46: Food Allergens and Regulations (Con
Page 47 and 48: Petition Process (if Labeling is Ma
Page 49 and 50: Analytical Methods to Detect Food A
Page 51 and 52: Protein Fining Agents for Wine •
Page 53 and 54: ELISA Technology • Enzyme-linked
Page 55 and 56: Egg Protein Assay ELISA Kits (Conti
Page 57 and 58: Egg Kit Responses to Wine Spiked wi
Page 59 and 60: Response of Egg RM vs. Time Henning
Page 61 and 62: Wheat Allergens • Gluten is group
Page 63 and 64: Analyzed Malt Beverage Samples •
Page 65 and 66: Gluten Assay ELISA Kits (Continued)
Page 67 and 68: Sample Preparation • Extract malt
Page 69 and 70: Results (Continued) Gluten Conc (pp
Page 71 and 72: Results (Continued) Comparison of G
Page 73 and 74: Results (Continued) Gluten Conc (pp
Page 75 and 76: Non-ELISA Detection of Food Allerge
Page 77 and 78: Protein Detection by Mass Spectrome
Page 79 and 80:
Conclusions • TTB mandated by Con
Page 81:
Contact Information Beverage Alcoho
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Prohibited Ingredients - TTB

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