21.01.2015 Views

人疱疹病毒6 型(HHV-6)酶联免疫分析( ELISA) 试剂盒使用说明书

人疱疹病毒6 型(HHV-6)酶联免疫分析( ELISA) 试剂盒使用说明书

人疱疹病毒6 型(HHV-6)酶联免疫分析( ELISA) 试剂盒使用说明书

SHOW MORE
SHOW LESS

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Human <strong>HHV</strong>-6<br />

FOR RESEARCH USE ONLY<br />

Drug Names<br />

Generic Name:Human <strong>HHV</strong>-6 ELISA Kit.<br />

Purpose<br />

This kit allows for the determination of <strong>HHV</strong>-6 concentrations in Human serum, blood<br />

plasma, and other biological fluids.<br />

Principle of the assay<br />

The kit assay Human <strong>HHV</strong>-6 level in the sample,use Purified Human <strong>HHV</strong>-6 antibody to<br />

coat microtiter plate wells, make solid-phase antibody, then add <strong>HHV</strong>-6 to wells, Combined<br />

<strong>HHV</strong>-6 antibody which With HRP labeled , become antibody - antigen - enzyme-antibody<br />

complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue<br />

color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid<br />

solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.<br />

The concentration of <strong>HHV</strong>-6 in the samples is then determined by comparing the O.D. of the<br />

samples to the standard curve.<br />

4

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!