An Introduction to PCR and Quantitative PCR
An Introduction to PCR and Quantitative PCR
An Introduction to PCR and Quantitative PCR
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<strong>Quantitative</strong> Real Time <strong>PCR</strong><br />
• Definition: Assay that moni<strong>to</strong>rs the<br />
accumulation of a DNA product from a<br />
<strong>PCR</strong> reaction.<br />
– Quantitate the initial number of copies of a<br />
particular DNA in a sample.<br />
– Benefits from improved sensitivity,<br />
reproducibility, dynamic range, throughput,<br />
cost.
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