An Introduction to PCR and Quantitative PCR

More documents

Recommendations

Info

<strong>Quantitative</strong> <strong>PCR</strong> Chemistries dsDNA Binding Probe Based Detection • SYBR Green I TM • TaqMan ® • Molecular Beacons • Lux ® primers • Hybridization probes • Scorpions TM • Amplifluor ® probes • FRET
SYBR Green I DNA + free dye (weak fluorophore) Binds minor groove dsDNA (fluorescence ↑1,000x)
Page 1 and 2: An Introduction to PCR and Quantita
Page 3 and 4: Polymerase Chain Reaction Introduce
Page 5 and 6: Polymerase Chain Reaction
Page 7 and 8: Phases of PCR Plateau phase [DNA] l
Page 9 and 10: Detection of Insertion using PCR gD
Page 11 and 12: Quantitative Real Time PCR • Defi
Page 13 and 14: PCR Molecular Mechanism • Exponen
Page 15 and 16: Quantitative PCR [DNA] Threshold C
Page 17: Fluorescence Detection light light
Page 21 and 22: SYBR Green I Detection
Page 23 and 24: End-Point Melt Curve Detection Cont
Page 25 and 26: 4-Fluor Multiplex-Standard Curves
Page 27 and 28: QPCR for GMO Detection •Dilution
Page 29 and 30: QPCR for GMO Detection < lowest dil
Page 31 and 32: Standard Curve
Page 33: Conclusion •PCR is a invaluable t

An Introduction to PCR and Quantitative PCR

Create successful ePaper yourself

Delete template?

Save as template?