An Introduction to PCR and Quantitative PCR
An Introduction to PCR and Quantitative PCR
An Introduction to PCR and Quantitative PCR
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<strong>PCR</strong> Molecular Mechanism<br />
• Exponential amplification of the original DNA<br />
sequence (template) <strong>to</strong> create copies of part of the<br />
sequence (amplicon)<br />
X n =X 0 (1+E) n<br />
X = DNA concentration<br />
X 0 = Starting DNA concentration<br />
X n = DNA concentration at cycle n<br />
E = Efficiency of <strong>PCR</strong> reaction, 0-1
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