An Introduction to PCR and Quantitative PCR
An Introduction to PCR and Quantitative PCR
An Introduction to PCR and Quantitative PCR
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Q<strong>PCR</strong> Amplification Plot<br />
Fluorescence (R)<br />
Baseline<br />
Amplification<br />
Threshold<br />
Ct<br />
Cycle #<br />
C t = Fractional <strong>PCR</strong> cycle number at which<br />
the fluorescence intensity crosses the<br />
established threshold line.
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