JAFES-Booklet (English - pdf - 1103 Kb) - MEMS

Gene Expression Profiling Associated With Diabetic
Nephroapthy In Malay Type 2 Diabetes Mellitus
Faradianna EL a , Wan Nazaimoon WM a , Aziz Al-Safi b , Norlaila M c ,
Amir S Khir d , Zanariah H e , Wan Mohamad WB b
a Institute for Medical Research, , b HUSM, c HUKM, d Penang Medical College, e Hospital Putrajaya
The gene expression profiling of 308 samples obtained from Malay Type 2 Diabetes Mellitus patients
were analyzed using Affymetrix Hugene 1.0ST Array system. There were 82 patients with microalbuminuria
(MI), 59 with macroalbuminuria (MA), 41 with End-Stage-Renal-Failure (ESRD) and 126 with
normoalbuminuria (controls). Using Partek Analysis Software, MI was found to be associated with
up-regulation of 46 genes and down-regulation of 11 genes while 3043 genes were up-regulated and
774 genes were down-regulated in MA. ESRD caused up-regulation of 148 genes and downregulation
of 38 genes. Consistent with other studies, we found significant up-regulation of phosphatidylinositol
(PICALM) and major protein histocompatibility complex (HLA), and down-regulation
of bone morphogenic protein kinase (BPM2K) in association with progressive kidney malfunction.
These genes are involved in lipid different biological functions such as metabolic process, antigen
presentation and TGF- B growth factor. Interestingly in ESRD, there was a significant down-regulation
of the TTN gene which encodes the giant skeletal-muscle protein, titin. Further analysis will be
carried out to determine the significance of these findings.
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