UNIVERSITY OF KERALA

Selection and isolation of bacteria eg: Isolation of bacteria capable of degrading PAH from oil contaminated
earth.
Isolation and characterization of bacteria from leaf tissues, leaf rot etc.
Testing of microbial capacity to produce biologically active substances
Taxonomic classification of isolated microbes
Long and short term storage of microbes ( bacteria and fungi)
Isolation of fungal and plant protoplasts
Protoplast fusion ( PEG mediated)
Study of density gradient centrifugation (using CsCl and sucrose) for isolation of cell components.
Cryopreservation of cells and recovery of frozen stocks into culture.
Principles of microscopy, phase contrast and fluroscent microscopy
Staining: Gram, Giemsa , Trypan blue
Cytotoxicity assays-L3HJ Thymidine,
Constituents of blood, blood smear
Separation of plant pigments by TLC
Osmosis and Tonicity, slide identification of different cells,
Mitosis in onion root tip.
Cell culture techniques- plant cell culture only.
Microbiological examination of water.
Biochemical tests:
IMVIC test, Catalase test, Coagulase test, Gelatinase test, Oxidase test and other related tests.
REFERENCES
1. Benson, Microbiological and applications, Laboratory, Manual in General Microbiology Mc Graw Hill
Publications
2. Gunasekharan P, Laboratory manual in Microbiology, New Age nternational Publishers.
3. Cappucin J.G and N.Sherman, A Laboratory Manual, 4 th edition, Addison and Weslay.
FOURTH SEMESTER
08.401 ENGINEERING MATHEMATICS III
(CMPUNERFHB)
Credits: 04 L/T/P: 3/1/0
MODULE I
Complex Differentiation: Limits, continuity and differentiation of complex functions. Analytic functions-Cauchy
Reimann equations in Cartesian form (proof of necessary part only) properties of analytic functions-harmonic
functions. Milne Thomson method
Conformal mapping: The Transformations w=1/ z , w=z 2 , w=z+1/ z , w=sin z ,w=cos z ,Bilinear transformation
MODULE II
Complex Integration: Line integral- Cauchy’s integral theorem-Cauchy’s integral formula. Power series-radius of
convergence-Taylors and Laurents series-zeros and singularities –Residues and residue theorem. Evaluation of real
definite integrals-
2
0
f sin,
cos
d
, f x dx
MODULE III
with no poles of
f z
on the real axis (proof of theorems not required)
Numerical Techniques: Errors in numerical computation-solution of algebraic and transcendental equations by
bisection method, regula false method, Newton- Raphson method. Solution linear systems by Gauss elimination and
Gauss-Seidal method. Newton’s forward and backward interpolation formula. Lagranges interpolation formula.
Numerical integration. Trapezoidal and Simpson’s rule. Numerical solution of ODE Taylor series method,
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