What: Next Generation Sequencing seminars from Illumina Where ...

What: Next Generation Sequencing seminars from Illumina
Where : Seminar Room, 9th floor, Nanotechnology Building
When: 25/11/10 9:00 AM
Seminar 1: Dr. Abizar Lakdawalla "Using 2nd generation
sequencing technology for an unprecedented view of the
functional genome"
Seminar 2, (right after the first one): Dr. Lukas Smink " Next
Generation Sequencing Data Analysis"
Abstract seminar 1:
2nd generation sequencing technology has transformed our
understanding of the basic tenets of biology.
Mr. Abizar Lakdawalla, Industry Segment Manager-Sequencing
Expert, Illumina
In this seminar, we will discuss the most effective strategies for
genome variation discovery, DNA methylation analyses, and
transcriptional profiling and discovery on the Illumina sequencing
platforms. In addition we will present novel applications such as
determining protein translational profiles and analyzing RNA from
formalin fixed paraffin embedded tissue sections.
Contents:
1. Illumina sequencing technology – brief overview
2. Sequencing genomes and methylomes at variable coverage for
maximum cost effectiveness
3. Analyzing the COMPLETE transcriptome – coding and noncoding
from small samples and from FFPE
4. Novel applications – RNAi targets, protein translation, proteinnucleic
acid interactions
Abstract seminar 2:
Illumina Next Generation Sequencing Data analysis
Dr Ir Luc J Smink, Sr. Bioinformatics Support Scientist,
Scientific and Technical Support
The Illumina sequencing analysis can be split up in primary and
secondary analysis. The primary analysis takes place on the

instrument computer and involves the analysis of the images and
the basecalling and associated quality scoring. All primary
analysis process will be discussed in detail, as well as the
alternative basecalling using the off-line basecaller.
The Illumina secondary analysis is performed by the CASAVA
software which is able to demultiplex runs which have multiple
samples in a single lane, align using the GERALD alignment
program, perform variant detection and counting studies for RNA
sequencing.
For more information:
Dr. Erez Levanion