Presentation - Association of Public Analysts

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DNA fingerprinting of Basmati A: mixture of Pusa and Taraori Basmati and non-Basmati B: Pusa Basmati C: Super Basmati Markers: RM201 (CT) 17 RM171 (GATG) 5 RM229 (TC) 11 (CT) 5 C 3 (CT) 5 Method: amplification of marker loci with fluorescently labelled primers and analysis of amplified DNA on an ABI Prism 3100 sequencer (capillary electrophoresis). Green channel: JOE (6-Carboxy-4,5-Dichloro-2,7-Dimethoxy-Fluorescein) Blue channel: FAM (6-Carboxylfluorescein) October 2009 Association of Public Analysts Conference 2009 12
Quantitative evaluation of DNA fingerprint Pusa Taraori Sherbati RM1 RM223 M16 RM202 RM44 RM201 RM229 RM241 W W Y Y Y X X Y W W Z Y Y X Y Y W (Z) Z (Y) Y Y W Z X X (Y) RM171 Z Y W (Z,Y) RM55 Y X Y Main W 72% W 52% Y 91% X 60% W 48% X 51% X 43% Y 98% W 43% Y 87% Minor Z 24% Y 39% Z 9% Y 40% Y 52% W 32% X+2 31% Z 2% Z 41% X 12% Y 4% Z 9% _ 0% _ 0% _ 0% Z 18% Y+2 16% _ 0% Y 10% Z+2 1% _ 0% _ 0% _ 0% _ 0% _ 0% _ 0% Y 10% _ 0% X 5% _ 0% Main W 31% W 11% Y 50% X 59% W 48% X 10% X 2% Y 57% W 43% Y 46% Minor Z 24% Y 39% Z 9% Y Y 11% W 32% X+2 31% Z 2% Z X 12% Y 4% Z 9% _ 0% _ 0% _ 0% Z 18% Y+2 16% _ 0% Y 10% Z+2 1% _ 0% _ 0% _ 0% _ 0% _ 0% _ 0% Y 10% _ 0% X 5% _ 0% Main W 22% W 2% Y 50% X 50% W 48% X 1% X 2% Y 48% W 43% Y 46% Minor Z 24% Y 39% Z Y Y 2% W 32% X+2 31% Z 2% Z X 3% Y 4% Z 9% _ 0% _ 0% _ 0% Z 18% Y+2 16% _ 0% Y 1% Z+2 1% _ 0% _ 0% _ 0% _ 0% _ 0% _ 0% Y 1% _ 0% X 5% _ 0% Result: Content (%) Pusa 41% Taraori 9% Sherbati (non Basmati) 2% Other non Basmati not identified 48% Total Basmati 50% Total non Basmati 50% October 2009 Association of Public Analysts Conference 2009 13
Page 1 and 2: Authenticity of Rice W.Nader, R.Sch
Page 3 and 4: Current issues with rice Nr. Topic
Page 5 and 6: Basmati Varieties Taraori Basmati 3
Page 7 and 8: Approved Basmati varieties Basmati
Page 9 and 10: Results from ÖKOTEST Study, 2006 O
Page 11: Alleles of microsatellite markers f
Page 15 and 16: Validation at Eurofins for ISO 1702
Page 17 and 18: Results from the FSA quantitative r
Page 19 and 20: Quarantine area in Indian rice mill
Page 21 and 22: Stuffing and discharge of container
Page 23 and 24: Structure of the fragrance gene (fg
Page 25 and 26: Further applications of DNA fingerp
Page 27: Content Pusa, measured (%) Accuracy

Presentation - Association of Public Analysts

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