ACE Catalogue 2006/7

PREP
ACE Preparative HPLC Columns
ACE Preparative HPLC Columns
■ Ultra high purity base deactivated silica
■ 5, 10 and 15µm particle sizes available
■ Fully validated columns
■ Exceptional reproducibility
■ Excellent efficiencies
■ High sample recovery
■ Excellent column lifetime
■ 100Å and 300Å pore sizes
Achieve Reproducible High Performance Preparative Separations
Chromatographers with experience in preparative HPLC
know that resolution and loadability are of the utmost
importance. The greater the resolution, the higher the
sample load and the faster pure compound is obtained.
The ability to optimize resolution at the preparative scale
means starting with high performance separations at the
analytical scale. The same features that make ACE ultrainert
base deactivated analytical columns the choice of
method development chemists also make them the ideal
choice for scale-up and process methods.
Figure 32. Reproducible Scale-Up with ACE C18 Columns
ACE 3 C18
ACE 5 C18
ACE 10 C18
1
1
1
2
3
2 4
3
2 4
3
4
5
5
5
Sample: 1) Uracil 2) 4-Hydroxybenzoic acid 3) Acetylsalicylic acid 4) Benzoic acid 5) 2-Hydroxybenzoic acid 6) Ethyl paraben
Mobile Phase: 35:65 MeCN/0.1% TFA in H2O, Temperature: 22°C, Wavelength: 254nm
32
ACE preparative HPLC columns offer the following
benefits:
■ Loadability – high surface area and carbon load for
maximum sample capacity
■ Selectivity – available in 7 bonded phases to optimize
sample capacity
■ Rugged – reliable, long-term performance
■ Guaranteed reproducibility – complete column/batch
validation as for ACE analytical columns
■ Guard cartridges – available for maximum column
protection
6
6
6
LC/MS
150 x 1.0mm i.d.
0.05 ml/min
capacity 10µg
Analytical
150 x 4.6mm i.d.
1.00 ml/min
capacity 200µg
Preparative
150 x 21.2mm i.d.
21.2 ml/min
capacity 4-50mg