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<strong>ACE</strong> - Excellent Chromatographic Performance<br />
<strong>ACE</strong> Ultra Inert Base Deactivated HPLC Columns<br />
<strong>ACE</strong> columns are available in capillary through to preparative<br />
dimensions, with a wide range of particle sizes (3, 5, 10 and 15µm),<br />
pore sizes (100Å and 300Å) and surface chemistries (C18, C8, C4,<br />
CN, Phenyl and AQ).<br />
<strong>ACE</strong> ultra inert HPLC columns are designed to meet<br />
even the most challenging of chromatographic<br />
applications, giving excellent performance with acidic,<br />
basic and neutral molecules. Excellent column<br />
performance (up to 200,000 plates/metre) and<br />
reproducible chromatography are ensured by the<br />
most stringent of validation protocols.<br />
Independent comparison tests (including those shown below and also on pages 3, 12-13, 19, 23 and 32) show <strong>ACE</strong><br />
columns give outstanding performance.<br />
Independent Comparison of HPLC Columns #1<br />
Comparison of Stationary Phase Technologies<br />
- Independently tested at The School of Pharmacy, University of Sunderland, UK<br />
■ Leading Silica, Hybrid and Monolith Column Brands Tested<br />
■ 50 x 4.6mm Rapid Analysis Dimensions<br />
■ Inertness testing with basic molecules<br />
1<br />
Conclusion:<br />
<strong>ACE</strong> 3 C18<br />
(Ultra Pure Silica)<br />
2<br />
3<br />
4<br />
N0.1 = 4900 pl<br />
AS = 1.05<br />
5<br />
XTerra MS 3.5 C18<br />
(Hybrid)<br />
Sample: 1) Norephedrine 2) Nortriptyline 3) Toluene 4) Imipramine 5) Amitriptyline, Mobile Phase: 65:35 MeOH/25mM KH2PO4 (pH6.0)<br />
Column Dimensions: 50 x 4.6mm i.d., Flow Rate: 1.0ml/min Temperature: 22°C, Wavelength: 215nm<br />
Significant differences in efficiency, peak shape and selectivity are observed when<br />
analyzing basic molecules.<br />
Increased tailing and retention are indicative of undesirable secondary interactions<br />
between the test probes and silanol groups on the stationary phase surface. These<br />
interactions can also result in poor column reproducibility.<br />
<strong>ACE</strong> HPLC columns were independently tested and found to be the highest<br />
efficiency, most inert columns available – making them the #1 choice in the<br />
development of reproducible HPLC methods.<br />
1<br />
2<br />
3<br />
2<br />
4<br />
N0.1 = 2100 pl<br />
AS = 1.26<br />
5<br />
Chromolith SpeedRod RP-18e<br />
(Monolith)<br />
3 2<br />
1 5<br />
4<br />
N0.1 = 300 pl<br />
AS = 2.74<br />
Si-OH<br />
<strong>ACE</strong> ® Stationary<br />
Phases Virtually<br />
Eliminate the<br />
Negative Effects<br />
of Silanols on HPLC<br />
Separations
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