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Conditions<br />
Applications<br />
Cold Medicine Components<br />
Column: <strong>ACE</strong> 5 C18, 250 x 4.6mm<br />
Part Number: <strong>ACE</strong>-121-2546<br />
Mobile Phase: 50:50 MeOH/50mM KH 2PO 4<br />
(pH 3.0)<br />
Flow Rate: 1.0ml/min<br />
Temperature: 22°C<br />
Detection: UV, 220nm<br />
App. No. 1006<br />
Hippuric Acid<br />
Conditions<br />
Column: <strong>ACE</strong> 5 C18, 150 x 4.6mm<br />
Part Number: <strong>ACE</strong>-121-1546<br />
Mobile Phase: 85:15 10mM KH 2PO 4<br />
(pH 3.5)/MeCN<br />
Flow Rate: 1.0ml/min<br />
Temperature: Ambient<br />
Detection: UV, 254nm<br />
App. No. 1010<br />
1<br />
2<br />
3<br />
Time - Minutes<br />
1<br />
Compounds<br />
1. Vitamin C<br />
2. Acetaminophen<br />
3. Caffeine<br />
4. Aspirin<br />
5. Ethenzamide<br />
4<br />
Compounds<br />
1. Hippuric acid<br />
2. 2-Methylhippuric acid<br />
2<br />
5<br />
42<br />
Cyclosporin Mixture<br />
Conditions<br />
Column: <strong>ACE</strong> 5 C18, 250 x 3.0mm<br />
Part Number: <strong>ACE</strong>-121-2503<br />
Mobile Phase: 46:51:3:0.1<br />
H 2O/MeCN/MTBE/H 3PO 4<br />
Flow Rate: 0.8ml/min<br />
Temperature: 80°C<br />
Detection: UV, 210nm<br />
App. No. 1037<br />
1<br />
Insulins<br />
Conditions<br />
Column: <strong>ACE</strong> 5 C18, 250 x 4.6mm<br />
Part Number: <strong>ACE</strong>-121-2546<br />
Mobile Phase: A. 29:71 MeCN/H 2O + 0.1% TFA<br />
B. 32:68 MeCN/H 2O + 0.1% TFA<br />
Flow Rate: 1.0ml/min<br />
Gradient: T(mins) %A %B<br />
0 90 10<br />
16 10 90<br />
Temperature: Ambient<br />
Detection: UV, 215nm<br />
App. No. 1011<br />
2<br />
3<br />
4<br />
5<br />
6<br />
Compounds<br />
1. Isocyclosporin A<br />
2. Cyclosporin C<br />
3. Cyclosporin B<br />
4. Cyclosporin L<br />
5. Cyclosporin U<br />
6. Cyclosporin A<br />
7. Dihydrocyclosporin A<br />
8. Cyclosporin G<br />
9. Cyclosporin D<br />
10.Cyclosporin E<br />
7 8<br />
Reproduced with kind permission of Camurus AB, Lund, Sweden<br />
Time - Minutes Time - Minutes<br />
9<br />
Compounds<br />
1. Bovine insulin<br />
2. Human insulin<br />
3. Porcine insulin<br />
1<br />
2<br />
10<br />
3
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