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Dietary Fibre - ILSI Argentina

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<strong>Dietary</strong> <strong>Fibre</strong> 19<br />

A variable proportion of dietary fibre will be derived<br />

from isolated or synthetic indigestible carbohydrates,<br />

incorporated into food products, for example, nondigestible<br />

oligosaccharides (fructo-oligosaccharides,<br />

galacto-oligosaccharides), resistant starch, resistant<br />

maltodextrins and polydextrose (Table 7).<br />

TABLE 7<br />

Some synthetic and modified fibres<br />

<strong>Fibre</strong> component<br />

Fructo-oligosaccharides<br />

Oligofructose<br />

Galacto-oligosaccharides<br />

Gluco-oligosaccharides<br />

Xylo-oligosaccharides<br />

Polydextrose<br />

Resistant maltodextrins<br />

Resistant starches<br />

Production process<br />

Transfructosylation of sucrose with a<br />

ß-fructosidase of Aspergillus niger<br />

Partial enzymatic degradation of<br />

native plant inulin<br />

Enzymatic transgalactosylation of<br />

lactose<br />

Transglycosylation using<br />

dextransucrase from Leuconostoc<br />

mesenteroides<br />

Partial enzymatic hydrolysis of xylan<br />

by xylanase from Trichoderma sp.<br />

Thermal polymerisation of glucose<br />

with sorbitol and acid (as a catalyst)<br />

Alkaline heat treatment of starch<br />

Retrogradation of high amylose starch<br />

ANALYSIS<br />

As noted above, no single analytical method is able to<br />

measure all fibre components in foods. There is a need<br />

for analytical methods that quantify the components in<br />

the dietary fibre definition, and at the same time<br />

excludes other food components. Box 1 and Table 1<br />

describe the principal methods of analysis for dietary<br />

fibre currently in use.<br />

Since 1985, the main accepted official method for<br />

measurement of total fibre has been the enzymaticgravimetric<br />

method of the AOAC (no. 985.29). However,<br />

as the definition of dietary fibre has evolved and a range<br />

of other AOAC and AACC approved methods have been<br />

adopted, total dietary fibre methods do not quantify the<br />

wider range of components now considered as dietary<br />

fibre.<br />

In the UK, for the purposes of food composition tables,<br />

dietary fibre is measured as non-starch polysaccharides<br />

by the Englyst method (Box 1). However, the AOAC<br />

method (985.29) generates significantly higher values for<br />

dietary fibre than the Englyst method, particularly for<br />

foods rich in starch such as potato, bread, beans and<br />

cornflakes. This results from the recovery of some of the<br />

RS3 fraction of resistant starch as dietary fibre. In foods<br />

containing lignin, such as whole grain cereals, the fact<br />

that lignin is not included in the non-starch polysaccharides<br />

analysis contributes further to the lower<br />

values for this method. Therefore it is important to<br />

recognise that where the Englyst values are used in food<br />

tables and as the basis for dietary recommendations, as in<br />

the UK, values will not always coincide with the declared<br />

values on food product labels, which are based on the<br />

enzymatic-gravimetric AOAC method.

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