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Azoxystrobin Activity on Rhizoctonia solani and its Efficacy ... - Iresa

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foliar applicati<strong>on</strong> of azoxystrobin<br />

effectively c<strong>on</strong>trol the diseases incidence<br />

like the agents of southern stem rot<br />

(Sclerotium rolfsii), head blight (Fusarium<br />

graminearum), <strong>and</strong> leather rot<br />

(Phytophthora cactorum) (14, 5, 13).<br />

<str<strong>on</strong>g>Azoxystrobin</str<strong>on</strong>g> means recovery from<br />

three fortified (0.05, 0.1 <strong>and</strong> 0.2 ppm) rice<br />

matrices (grain, straw, husk <strong>and</strong> bran) were<br />

86.96%, 84.71% <strong>and</strong> 82.36%, respectively<br />

(Table 3). Therefore, under the specified<br />

c<strong>on</strong>diti<strong>on</strong>s no interfering peaks were<br />

observed <strong>on</strong> the chromatogram of the<br />

samples. The instrument did not detect<br />

residues of azoxystrobin even at the<br />

highest applied c<strong>on</strong>centrati<strong>on</strong>. Then, we<br />

can suppose that the residues of<br />

azoxystrobin in rice matrices were below<br />

the limit of detecti<strong>on</strong> of the instrument<br />

(0.004 µg/g of sample). C<strong>on</strong>sidering the<br />

U.S. Envir<strong>on</strong>mental Protecti<strong>on</strong> Agency<br />

(EPA), the maximum residue lim<strong>its</strong><br />

(MRLs) for azoxystrobin in rice grain,<br />

straw <strong>and</strong> hulls are, respectively 5, 12 <strong>and</strong><br />

20 ppm (7). Therefore, it seems that<br />

azoxystrobin in our trials was rapidly<br />

metabolized in rice plants. Chen et al. (2)<br />

obtained a similar result in Chinese<br />

cabbage. Hence, foliar applicati<strong>on</strong> of<br />

azoxystrobin to c<strong>on</strong>trol sheath blight is<br />

very unlikely to cause acute <strong>and</strong> chr<strong>on</strong>ic<br />

health risk.<br />

Table 3. Recovery percentage of azoxystrobin in fortified rice samples<br />

Rice matrice<br />

<str<strong>on</strong>g>Azoxystrobin</str<strong>on</strong>g> recovery (%)<br />

0.05 ppm 0.1 ppm 0.2 ppm<br />

Mean<br />

recovery<br />

(%)<br />

Recovery<br />

factor<br />

(RF)<br />

Grains 80.12 82.86 97.91 86.96 1.15<br />

Straw 85.12 81.32 87.69 84.71 1.18<br />

Bran & Husk 80.00 84.90 82.18 82.36 1.21<br />

* Determinability of the instrument = 0.004 µg/g of sample.<br />

____________________________________________________________________________<br />

RESUME<br />

Sundravadana S., Alice D., Kuttalam S., et Samiyappan R. 2007. Activité de l’azoxystrobine sur<br />

Rhizoct<strong>on</strong>ia <strong>solani</strong> et s<strong>on</strong> efficacité c<strong>on</strong>tre la brûlure de la gaine foliaire du riz. Tunisian Journal of<br />

Plant Protecti<strong>on</strong> 2: 79-84.<br />

La brûlure de la gaine foliaire, causée par Rhizoct<strong>on</strong>ia <strong>solani</strong>, est l’une des principales maladies du riz.<br />

Nous av<strong>on</strong>s étudié l’effet de l’azoxystrobine sur la croissance in vitro de R. <strong>solani</strong> et s<strong>on</strong> efficacité c<strong>on</strong>tre<br />

cette maladie en plein champ. Les résultats <strong>on</strong>t révélé que l’azoxystrobine à 1, 2 et 4 ppm, inhibe<br />

complètement la croissance mycélienne de R. <strong>solani</strong>. L’étude au champ a m<strong>on</strong>tré que la pulvérisati<strong>on</strong><br />

foliaire de l’azoxystrobine à 125, 250 et 500 g/ha supprime significativement (> 64%) le développement<br />

de la brûlure de la gaine foliaire et augmente le rendement (> 60%). Pour c<strong>on</strong>trôler cette maladie, la dose<br />

optimale d’azoxystrobine était de 125 g/ha. Nos résultats <strong>on</strong>t indiqué aussi qu’il n’y avait pas de résidus<br />

dans les plantes du riz au moment de la récolte et pas de phytotoxicité avec la plus haute c<strong>on</strong>centrati<strong>on</strong><br />

d’azoxystrobine (500 g/ha).<br />

Mots clés : <str<strong>on</strong>g>Azoxystrobin</str<strong>on</strong>g>e, brûlure de la gaine foliaire, croissance mycélienne, lutte chimique, résidus,<br />

Rhizoct<strong>on</strong>ia <strong>solani</strong>, riz<br />

_____________________________________________________________________________<br />

Tunisian Journal of Plant Protecti<strong>on</strong> 82<br />

Vol. 2, No. 2, 2007

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