The impact of Ty3-gypsy group LTR retrotransposons ... - URGV - Inra

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Figure Legends Figure 1 - FISH of mitotic metaphase chromosomes of Triticum and Aegilops species. The species analysed are (a–c) T. aestivum cv. Chinese Spring; (d) Ae. speltoides and T. urartu; (e) Ae. speltoides and Ae. tauschii. The probe combinations are: (A, C–E) BAC clone 2383A24 (green); (B) 2383A24/15 (green); (A and B) pSc119.2 (red); (C and E) Ae. tauschii DNA (red). Arrows point to the translocation of 7BS to 4AL. Figure 2 - Structural organisation of 113 605-bp T. aestivum genomic region marked by Spelt1 subtelomeric repeats. The genomic region contains B-genome specific Fatima sequences (p at the ends of the names of transposable elements indicates that the corresponding elements are truncated). Figure 3. The comparison of “autonomous” and “nonautonomous” variants of Fatima. The “autonomous” variant TREP3189 presented by the consensus nucleotide sequence, with the two open reading frames corresponding to hypothetical proteins PTREP233 (polyprotein) and PTREP234. The “nonautonomous” variant TREP3198 presented by the consensus nucleotide sequence, with the open reading frames corresponding to hypothetical proteins PTREP231 (polyprotein) and PTREP232. The conservative domains are indicated as follows: AP – aspartic proteinase, RT – reverse transcriptase, RH – RNAse H, INT – integrase, and gag – structural core protein. The conserved regions between “autonomous” and “nonautonomous” variants are indicated with light grey shading and the percent of homology is defined. Likewise the relative position of probe BAC2383A24/15 in reference to “autonomous” Fatima variant is marked with light grey; in “nonautonomous” variant, the sequence corresponding to BAC2383A24/15 is absent. Figure 4 - The neighbor-joining phylogenetic tree of autonomous Fatima elements originating from different Triticeae genomes. The phylogenetic tree was constructed using a CLUSTALW multiple alignment for the Fatima nucleotide sequences coding for RNase H. Bootstrap support over 50% is shown for the corresponding branches. Designations in sequence names: Ta, T. aestivum; Td, T. durum;
Tt, T. turgidum; Tu, T. urartu; Tm, T. monococcum; and Aet, Ae. tauschii. Insertion timing for Fatima elements is parenthesised. The group designated as B predominantly contains the elements belonging to the B genome; and D, the elements belonging to the D genome. The “mixed” group contains the Fatima elements from different Triticeae genomes.
Page 1 and 2: BMC Plant Biology This Provisional
Page 3 and 4: Abstract Background Transposable el
Page 5 and 6: allopolyploids, lines with foreign
Page 7 and 8: diploid genomes and the correspondi
Page 9 and 10: (the corresponding data were deposi
Page 11 and 12: Note that characteristic of BAC_238
Page 13 and 14: The regions homologous to the codin
Page 15 and 16: formed about 1 MYA, thereby suggest
Page 17 and 18: As has been demonstrated, the diplo
Page 19 and 20: BAC FISH data (Figure 1) and phylog
Page 21 and 22: The following specific primer pairs
Page 23 and 24: available in the TREP [42] and NCBI
Page 25 and 26: References 1. Von Sternberg RM, Nov
Page 27 and 28: 16. Mukai Y, Nakahara Y, Yamamoto M
Page 29 and 30: wheat: Evolutionary relationships a
Page 31: 49. Lim KY, Kovarik A, Matyasek R,
Page 35 and 36: Table 2 - The genes identified in n
Page 37 and 38: Figure 1
Page 39 and 40: Figure 3
Page 41: Additional files provided with this

The impact of Ty3-gypsy group LTR retrotransposons ... - URGV - Inra

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