Candida Infection Biology – fungal armoury, battlefields ... - FINSysB
Candida Infection Biology – fungal armoury, battlefields ... - FINSysB
Candida Infection Biology – fungal armoury, battlefields ... - FINSysB
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Poster number: 03<br />
The transcription factor Sko1 represses the yeast-tohypha<br />
transition and mediates the oxidative stress<br />
response in <strong>Candida</strong> albicans.<br />
Inês Correia, Elvira Román, David M. Arana, Daniel Prieto, Verónica<br />
Urrialde, César Nombela, Rebeca Alonso-Monge and Jesús Pla. §<br />
Departamento de Microbiología II. Facultad de Farmacia. Universidad Complutense de<br />
Madrid. Plaza de Ramón y Cajal s/n. E-28040 MADRID. SPAIN<br />
Cells adapt to external changes by triggering transcriptional responses. These<br />
responses are complex and are mediated by different transcription factors. Mutants<br />
lacking the transcription factor Sko1 were generated in <strong>Candida</strong> albicans and its<br />
roles on the physiology of this opportunistic pathogen are reported. Genome-wide<br />
transcriptional analysis revealed that Sko1 acts as a transcriptional repressor of<br />
genes involved in pathogenesis and hyphal formation. The sko1 mutant displayed<br />
an increased expression of the hyphal related genes ECE1 and HWP1 but showed<br />
no drastic alterations in the mouse model of systemic infection. Deletion of SKO1<br />
rendered mutants derepressed in the yeast-to-hypha transition. Moreover, Sko1<br />
was shown to be involved in the response to oxidative stress and sko1 mutants<br />
increased the sensitivity of hog1 mutants to the myelomonocytic cell line HL-60.<br />
Alterations in the phosphorylation pattern of MAP kinase mutants in response to<br />
oxidative challenge were also observed. Genome-wide transcriptional analysis after<br />
hydrogen peroxide treatment revealed that sko1 mutants were able to generate an<br />
adaptive response similar to wild type strains, although important differences were<br />
detected in the magnitude of such transcriptional response. Collectively, these<br />
results implicate Sko1 as a key mediator of the oxidative stress response and<br />
morphological transition in C. albicans.<br />
116