Candida Infection Biology – fungal armoury, battlefields ... - FINSysB

Poster number: 32
Human cells response to Non- Candida albicans Candida
infection
Cláudia Botelho 1 , Melyssa Negri 1 , Catarina Seabra 1 , Mariana Henriques 1 ,
Joana Azeredo 1 , Rosário Oliveira 1
1 Institute for Biotechnology and Bioengineering, Centre of Biological Engineering,
Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
This study evaluated the influence of adhered Candida spp (C.tropicalis ATCC 750,
C. parapsilosis ATCC 2201, C. glabrata ATCC 2001 and C. albicans SC5413) on the
biological behavior of human cells (HeLa and TCC-SUP). A yeast suspension was
added to a confluent layer of human cells and after 2 and 24h of incubation yeast
cells were detached for CFU counts. All Candida spp assayed adhered to epithelial
cells, although C. glabrata was the species adhering in higher extent to both
epithelial cell lines. From 2 to 24h there is a significant increase in the number of C.
glabrata adhered to TCC-SUP cells, while no increase in the number of C. glabrata
colonizing HeLa cells was observed. An opposite result was observed for C.
tropicalis; there was no increase in the number of adherent cells to TCC-SUP cells,
but there was an increase for HeLa cells. The metabolic activity of HeLa cells
decreased after a period of 24h of Candida infection, especially in the presence C.
tropicalis. The biological response of TCC-SUP cells was different from the one
observed with HeLa cells, the presence of C. albicans and C. parapsilosis induced
an increase in the metabolic activity of TCC-SUP cells, while C. tropicalis and C.
glabrata promoted a decrease.. After 24h of C. albicans adhesion, the highest
human cells damage was detected in HeLa cells (measured by LDH release), while
C.tropicalis seems to induce higher LDH release after 24h in contact with TCC-SUP
cells. The biological response of human cells showed to be dependent on their
origin and on the colonizing yeast species/strain, and no direct relation between
the number of adherent cells and the biological response was observed.
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