FACE® N-Linked Oligosaccharide Profiling Kit - ProZyme

SECTION 4
FACE ELECTROPHORESIS
I. Preparation of 1.5 liter of FACE Oligo Gel Running Buffer
(1.5 liter of buffer is sufficient for one electrophoresis run of one or two gels.)
NOTE:
FACE electrophoresis buffer should be prepared up to one day in advance of the
electrophoresis run and stored at 4°C.
1 Remove one of the Oligo Profiling Gel Running Buffer packs from the kit.
2 Cut open one end of the packet and carefully pour the contents into a 2-liter graduated cylinder and
add 1000 ml of distilled water.
3 Rinse buffer packet with 100 ml of water and add to the redissolved buffer.
4 Bring up to a final volume of 1.5 liter with water.
5 Mix well. The buffer must be chilled to 4-6°C before use.
II. Set-up of FACE Electrophoresis Apparatus
FACE Electrophoresis of N-linked gels is best performed at a temperature range of 5-8°C. We
recommend the use of a recirculating chiller, however satisfactory results can be obtained by chilling the
buffer prior to the electrophoresis run.
CAUTION:
To prevent damage to the gel box, connect both quick disconnect fittings to gel box
before turning on the recirculator!
1 Place the FACE electrophoresis tank containing a stir bar on a mechanical stirrer. If using a
recirculating chiller, connect it to the gel box cooling chamber with tubing and quick-connect
fittings. Turn on the circulator and stirrer; set the coolant temperature to 5ºC. Bleed all air out of
system by tilting the gel box in the direction of the outlet.
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