µl Spectrophotometer USER MANUAL Version 4.0/21/03/11 All ...

Nucleic Acid MeasurementsSelect the relevant nucleic acid mode (dsDNA, ssDNA, RNA, Protein, UV/Vis) from within your project folder inthe „Project Selector‟ window and click the button „New‟ under the heading Sample. A new window will openwhich displays a graph and table.Pipette 2.5ul of „Blank‟ solution into a tip and place the tip and pipette into the plastic pipette holder.Click the button marked „Blank‟. The software will attempt to blank the spectrophotometer and set thebaseline to zero at 260nm. A rapid clicking noise will be head for several seconds and the LED on the front ofthe unit will turn orange.If the unit has not warmed up or if there is residue/dust on the lens then the blanking may fail and a messagerequesting that you „re-blank‟, will be displayed. If this occurs simply try blanking again and if the procurecontinues to fail try following the lens cleaning protocol.Example of graph windowawaiting the „blank‟ solutionand message indicating that the„Blanking‟ was unsuccessful.19