RNA interference: traveling in the cell and gaining functions?

46Review TRENDS in Genetics Vol.19 No.1 January 2003produce effective RNA interference in adult Drosophila. Neuron 33,177–18434 Reed, R. and Hurt, E. (2002) A conserved mRNA export machinerycoupled to pre-mRNA splicing. Cell 108, 523–53135 Mette, M.F. et al. (2001) Resistance of RNA-mediated TGS to HC-Pro, aviral suppressor of PTGS, suggests alternative pathways for dsRNAprocessing. Curr. Biol. 11, 1119 – 112336 Sijen, T. et al. (2001) On the role of RNA amplification in dsRNAtriggeredgene silencing. Cell 107, 465–47637 Tijsterman, M. et al. (2002) RNA helicase MUT-14-dependent genesilencing triggered in C. elegans by short antisense RNAs. Science 295,694–69738 Dalmay, T. et al. (2000) An RNA-dependent RNA polymerase gene inArabidopsis is required for posttranscriptional gene silencingmediated by a transgene but not by a virus. Cell 101, 543–55339 Mourrain, P. et al. (2000) Arabidopsis SGS2 and SGS3 genes arerequired for posttranscriptional gene silencing and natural virusresistance. Cell 101, 533–54240 Martens, H. et al. (2002) RNAi in Dictyostelium: the role of RNAdirectedRNA polymerases and double-stranded RNase. Mol. Biol. Cell13, 445–45341 Lipardi, C. et al. (2001) RNAi as random degradative PCR: siRNAprimers convert mRNA into dsRNAs that are degraded to generatenew siRNAs. Cell 107, 297–30742 Béclin, C. et al. (2002) A branched pathway for transgene-inducedRNA silencing in plants. Curr. Biol. 12, 684–68843 Vaistij, F.E. et al. (2002) Spreading of RNA targeting and DNAmethylation in RNA silencing requires transcription of the target geneand a putative RNA-dependent RNA polymerase. Plant Cell 14,857–86744 Morel, J.-B. et al. (2000) DNA methylation and chromatin structureaffect transcriptional and post-transcriptional transgene silencing inArabidopsis. Curr. Biol. 10, 1591–159445 Rountree, M.R. and Selker, E.U. (1997) DNA methylation inhibitselongation but not initiation of transcription in Neurospora crassa.Genes Dev. 11, 2383–239546 Buhr, T. et al. (2002) Ribozyme termination of RNA transcripts downregulateseed fatty acid genes in transgenic soybean. Plant J. 30,155–16347 Pal-Bhadra, M. et al. (2002) RNAi related mechanisms affect bothtranscriptional and posttranscriptional transgene silencing in Drosophila.Mol. Cell 9, 315–32748 Cox, D.N. et al. (2000) piwi encodes a nucleoplasmic factor whoseactivity modulates the number and division rate of germline stem cells.Development 127, 503–51449 Volpe, T.A. et al. (2002) Regulation of heterochromatic silencing andhistone H3 lysine-9 methylation by RNAi. Science 297, 1833–183750 Simmer, F. et al. (2002) Loss of the putative RNA-directed RNApolymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr.Biol. 12, 1317–131951 Montgomery, M.K. et al. (1998) RNA as a target of double-strandedRNA-mediated genetic interference in Caenorhabditis elegans. Proc.Natl Acad. Sci. USA 95, 15502–1550752 Bosher, J.M. et al. (1999) RNA interference can target pre-mRNA:consequences for gene expression in a Caenorhabditis elegans operon.Genetics 153, 1245–125653 Wu-Scharf, D. et al. (2000) Transgene and transposon silencing inChlamydomonas reinhardtii by a DEAH-box RNA helicase. Science290, 1159–116254 Waterhouse, P.M. et al. (2001) Gene silencing as an adaptive defenceagainst viruses. Nature 411, 834–84255 Mourelatos, Z. et al. (2002) miRNPs: a novel class of ribonucleoproteinscontaining numerous microRNAs. Genes Dev. 16, 720–72856 Charroux, B. et al. (2000) Gemin4: a novel component of the SMNcomplex that is found in both gems and nucleoli. J. Cell Biol. 148,1177–118657 Llave, C. et al. (2002) Endogenous and silencing-associated smallRNAs in plants. Plant Cell 14, 1605–161958 Hamilton, A. et al. (2002) Two classes of short interfering RNA in RNAsilencing. EMBO J. 21, 4671–467959 Reinhart, B.J. et al. (2002) MicroRNAs in plants. Genes Dev. 16,1616–162660 Jacobsen, S.E. et al. (1999) Disruption of an RNA helicase/RNAse IIIgene in Arabidopsis causes unregulated cell division in floralmeristems. Development 126, 5231–524361 Papaefthimiou, I. et al. (2001) Replicating potato spindle tuber viroidRNA is accompanied by short RNA fragments that are characteristic ofpost-transcriptional gene silencing. Nucleic Acids Res. 29, 2395–240062 Mallory, A.C. et al. (2001) HC-Pro suppression of transgene silencingeliminates the small RNAs but not transgene methylation or themobile signal. Plant Cell 13, 571–58363 Dudley, N.R. et al. (2002) Using RNA interference to identify genesrequired for RNA interference. Proc. Natl Acad. Sci. USA 99,4191–419664 Cogoni, C. and Macino, G. (1999) Posttranscriptional gene silencing inNeurospora by a RecQ DNA helicase. Science 286, 2342–234565 Reinhardt, B.J. and Bartel, D.P. (2002) Small RNAs correspond tocentromere heterochromatic repeats. Science 297, 183166 Hall, I.M. et al. (2002) Establishment and maintenance of aheterochromatin domain. Science 297, 2232–223767 Mochizuki, K. et al. (2002) Analysis of a piwi-related gene implicatessmall RNAs in genome rearrangement in Tetrahymena. Cell 110,689–69968 Taverna, S.D. et al. (2002) Methylation of histone H3 at lysine 9 targetsprogrammed DNA elimination in Tetrahymena. Cell 110, 701–71169 Lachner, M. and Jenuwein, T. (2002) The many faces of histone lysinemethylation. Curr. Opin. Cell Biol. 14, 286–29870 Pasquinelli, A.E. (2002) MicroRNAs: deviants no longer. Trends Genet.18, 171–17371 Rhoades, M.W. et al. (2002) Prediction of plant microRNA targets. Cell110, 513–52072 Llave, C. et al. (2002) Cleavage of Scarecrow-like mRNA targetsdirected by a class of Arabidopsis miRNA. Science 297, 2053–205673 Lee, Y. et al. (2002) MicroRNA maturation: stepwise processing andsubcellular localization. EMBO J. 21, 4663–467074 Grishok, A. et al. (2001) Genes and mechanisms related to RNAinterference regulate expression of the small temporal RNAs thatcontrol C. elegans developmental timing. Cell 106, 23–3475 Aravin, A.A. et al. (2001) Double-stranded RNA-mediated silencingof genomic tandem repeats and transposable elements in theD. melanogaster germline. Curr. Biol. 11, 1017–1027http://tigs.trends.com