Luca Brunelli, M.D., Ph.D. - Pediatrics - University of Utah

Curriculum Vitae Last Updated: 08/12/2013PERSONAL DATAName: Luca BrunelliCitizenship: United StatesEDUCATIONYears Degree Institution (Area of Study)1982 - 1988 M.D. University of Genoa Medical School (Medicine)1988 - 1992 Resident Gaslini Children's Hospital, Univ. of Genoa (Pediatrics)1993 - 1994 Research Fellow University of Alabama (Anesthesiology)Birmingham, AL1995 - 1998 Resident University of Genoa (Anesthesiology and Intensive Care)1998 M.D. University of Genoa (Medicine)1998 - 1999 Fellow Duke University Medical Center (Neonatal-Perinatal Medicine)Durham, NC1999 - 2001 Resident University of Rochester (Pediatrics)Rochester, NY2000 - 2002 Ph.D. University of Turin (Biology of Oxygen Free Radicals)2001 - 2002 Externship The Philadelphia Child & Family Therapy Training CenterPhiladelphia, PA2001 - 2003 Fellow Thomas Jefferson University Hospital (Neonatal-Perinatal Medicine)Philadelphia, PABOARD CERTIFICATIONS01/01/2001 American Board of Pediatrics (Pediatrics), Certified11/17/2003 American Board of Pediatrics (Sub: Neo-Peri), CertifiedCURRENT LICENSES/CERTIFICATIONS1993 - Present Chancellor of the University of Genoa, Italy: National License - Physician (MD)1994 - Present ECFMG - Physician (MD)2008 - 2014 State License (UT) - Physician (MD)2008 - 2014 Controlled Substance (UT) - Physician (MD)ACADEMIC HISTORYPediatrics (Neonatology)07/01/2008 Hire, Tenure Track, - Clinician ScholarAssistant ProfessorPROFESSIONAL EXPERIENCEFull Time Positions1990 - 2002 Attending Physician, Gaslini Children's Hospital, Division of Anesthesia andIntensive Care, Genoa, ItalyBrunelli, Page 1

1995 Pediatric Consultant, Medical mission of the Institute for University Cooperation(ICU) under the auspices of the Italian Ministry of Foreign Affairs, Manila,Philippines2001 - 2003 Instructor, Jefferson Medical College, Philadelphia, PA2002 - 2003 Attending Physician, Gaslini Children's Hospital, Division of Neonatal Medicine,Genoa, Italy2003 - 2008 Attending Physician, Memorial Hermann Children's Hospital, Division of Neonatal-Perinatal Medicine, Houston, TX2003 - 2008 Assistant Professor of Pediatrics, The University of Texas Health Science Center atHouston, Houston, TX2008 - Present Attending Physician (Neonatology), University of Utah Medical Center, Salt LakeCity, Utah2008 - Present Attending Physician (Neonatology), Intermountain Medical Center, Murray, Utah2008 - Present Attending Physician (Neonatology), Primary Children's Medical Center, Salt LakeCity, Utah2008 - Present Assistant Professor of Pediatrics, The University of Utah, Salt Lake City, UTPart Time PositionsReviewer ExperienceReviewer for Developmental DynamicsReviewer for Human Molecular GeneticsReviewer for PLoS ONEReviewer for the Journal of Industrial Microbiology & BiotechnologySCHOLASTIC HONORS1988 Magna Cum Lade, Medical Doctor, University of Genoa, Genoa, Italy1993 USMLE step 1 (score top 10%ile)1994 USMLE step 2(score in top 15%ile)1999 Magna Cum Laude, Anesthiology Residency University of Genoa, Genoa, Italy2000 Gilbert Forbes Fellowship for Research. Strong Children's Research Center,University of Rochester Medical Center, Rochester, NY2001 Finalist, Thomas R. Boggs, Junior Research Award. Philadelphia Perinatal Society,Philadelphia, PA2002 13th Annual NICHD Conference on Maternal-Neonatal-Reproductive Medicine.Aspen, CO2003 American Sub-board of Neonatal-Perinatal Medicine (score top 15%ile)2013 Nominated for the Most Valuable Physician Award, Intermountain Medical CenterNICUADMINISTRATIVE EXPERIENCEAdministrative Duties2006 - 2008 Faculty Senate, University of Texas at Houston Medical School, Houston, TX2006 - 2007 Quality Review Control Committee, University of Texas at Houston Medical Center2010 - Present Retention Promotion and Tenure Committee, Department of Pediatrics, University ofUtah School of Medicine, Salt Lake City, UTBrunelli, Page 2

Professional Organization & Scientific Activities2013 - Present Organizer & Member, Society for Developmental Biology, SouthWest RegionalMeetingUNIVERSITY COMMUNITY ACTIVITIESCollege Level2010 - Present Member, Appointments, Retention, Promotion, & Tenure CommitteeCURRENT MEMBERSHIPS IN PROFESSIONAL SOCIETIESAmerican Academy of PediatricsAmerican Heart Association, Council on Basic ScienceAmerican Society for MicrobiologyFUNDINGActive GrantsPending Grants01/01/14 -12/31/1601/01/14 -12/31/1606/01/14 -05/30/17Identifying Disease-causing Regulatory VariantsPrincipal Investigator: Luca BrunelliDirect Costs: $140,000 Total Costs: $140,000American Heart AssociationRole: Principal InvestigatorA 14-3-3 Dependent Pathway Regulating Myocardial CompactionPrincipal Investigator: Luca BrunelliDirect Costs: $140,000 Total Costs: $140,000American Heart Association Western States AffiliateRole: Principal InvestigatorThe Genetic Causes of Isolated Congenital Diaphragmatic HerniaPrincipal Investigator: Luca BrunelliDirect Costs: $390,000 Total Costs: $390,000March of Dimes FoundationRole: Principal InvestigatorPast Grants01/01/09 - The Role of 14-3-3ε in Cardiac Development12/31/09 Principal Investigator: Luca BrunelliDirect Costs: $25,000 Total Costs: $25,000Primary Children's Medical Center FoundationRole: Principal Investigator07/01/09 - A Transcriptional Pathway Regulating Ventricular Morphogenesis06/30/11 Principal Investigator: Luca BrunelliDirect Costs: $140,000 Total Costs: $140,000American Heart Association Western States AffiliateRole: Principal Investigator07/01/10 - The Role of 14-3-3ε in Cardiac Development06/30/11 Principal Investigator: Luca BrunelliDirect Costs: $25,000 Total Costs: $25,000Primary Children's Medical Center FoundationRole: Principal Investigator01/01/11 - Genetic Causes of Congenital Diaphragmatic HerniaBrunelli, Page 3

06/30/13 Principal Investigator: Luca BrunelliUniversity of Utah Funding Incentive Seed GrantRole: Principal InvestigatorTEACHING RESPONSIBILITIES/ASSIGNMENTSClinical Teaching2008 - 2009 Pediatric Residents teaching during clinical service: necrotizing enterocolitis,congenital diaphragmatic hernia, feeding the very low birth weight infant,conventional mechanical ventilation, congenital cytomegalovirus infections, andnonimmune hydrops fetalis. Three lectures per week, half hour each for 3 weeks.2009 - 2010 Pediatric Residents teaching during clinical service: congenital diaphragmatic hernia,neonatal renal physiology, use of nasal cannula in the NICU, fluid requirements ofthe very low birth weight infant, and conventional mechanical ventilation. Threelectures per week, half hour each for 5 weeks.2010 - 2011 Pediatric Residents teaching during clinical service: intraventricular hemorrhage,pulmonary issues in newborns, presentation of congenital heart disease in newborns,feeding the very low birth weight infant, necrotizing enterocolitis, fluid requirementsof the very low birth weight infant, conventional mechanical ventilation, andretinopathy of prematurity. Three lectures per week, half hour each for 3 weeks.2011 - 2012 Pediatric Residnets teaching during clinical service: anemia in newborns, neonatalrenal physiology, meconium aspiration syndrome, feeding the very low birth weightinfant, use of nasal cannula in the NICU, and conventional mechanical ventilation.Three lectures per week, half hour each for 3 weeks.2012 - 2013 Pediatric Residents teaching during clinical service: meconium aspiration syndrome,necrotizing enterocolitis, intraventricular hemorrhage, use of blood gases in theNICU, feeding the very low birth weight infant, use of nasal cannula in the NICU,congenital cytomegalovirus infections, and nonimmune hydrops fetalis. Threelectures per week, half hour each for 8 weeks.2013 - 2014 Pediatric Residents teaching during clinical service: ventilatory management,physical exam for cardiac and respiratory conditions in the NICU, necrotizingenterocolitis, use of nasal cannula, and ethical issues in the NICU. Three lectures perweek, half hour each for 6 weeks.Laboratory Teaching2011 - Present Yasuhiro Kosaka, Ph.D., Senior Laboratory Specialist. All techniques and proceduresin the laboratory relating to mouse heart development.Trainee SupervisionFellow2013 - Present Supervisor, Hari Shanmugam M.D., University of Utah. Neonatology clinical fellow.Project title: Genetics and epidemiology of congenitial diaphragmatic hernia.PhD/Doctorate2010 - Present Supervisor, George Lezin, University of Utah. Teaching new post-doctoral fellow.Techniques and procedures in the laboratory relating to molecular biology work andrecombineering.Educational LecturesDidactic Lectures2008 - Present Instructor, University of Utah. Eight lectures in the laboratory of Dr. H. JosephBrunelli, Page 4

Yost on the role of 14-3-3ε in hearat development.2008 Instructor, University of Utah. Pediatric Research in Progress (RIP) lectures. Therole of 14-3-3ε in cardiac development.2010 Instructor, University of Utah. Pediatric Research in Progress (RIP) lecture: DNApurification techniques and recombineering.2010 Division of Neonatology Fellow Conference: Neonatal conventional mechanicalventilation.2011 Division of Neonatology Fellow Conference: Meconium Aspiration Syndrome.2011 Pediatric Residents Noon Conference: Pulmonary issues of the newborn.PEER-REVIEWED JOURNAL ARTICLES1. Tao S, Calza G, Lerzo F, Virgone A, Camassa N, Panizzon G, Brunelli L, Moretti R, Grasso P,Ghiggeri GM, et al. (1995). Activation of the intracellular glutathione system by oxydative stressduring cardiopulmonary bypass and myocardial perfusion. Perfusion, 10(1), 45-50.2. Brunelli L, Crow JP, Beckman JS. (1995). The comparative toxicity of nitric oxide and peroxynitriteto Escherichia coli. Arch Biochem Biophys, 316(1), 327-34.3. Brunelli L, Yermilov V, Beckman JS. (2001). Modulation of catalase peroxidatic and catalaticactivity by nitric oxide. Free Radic Biol Med, 30(7), 709-14.4. Brunelli L, Hamilton E, Davis JM, Koo HC, Joseph A, Kazzaz JA, Wolfson MR, Shaffer TH.(2006). Perfluorochemical liquids enhance delivery of superoxide dismutase to the lungs of juvenilerabbits. Pediatr Res, 60(1), 65-70.5. Brunelli L, Cieslik KA, Alcorn LA, Vatta M, Baldini A. (01/01/2007). PPARδ up-regulates 14-3-3εin human endothelial cells via C/EBPβ. Circ Res, 100(5), e59-71.6. Vatta M, Sinagra G, Brunelli L, Faulkner G. (2009). Remodeling of dystrophin and sarcomeric Z-band occurs in pediatric cardiomyopathies: a unifying mechanism for force transmission defect. JCardiovasc Med (Hagerstown), 10(2), 149-56.7. Li Z, Ai T, Samani K, Xi Y, Tzeng HP, Xie M, Wu S, Ge S, Taylor MD, Dong JW, Cheng J,Ackerman MJ, Kimura A, Sinagra G, Brunelli L, Faulkner G, Vatta M. (2010). A ZASP missensemutation, S196L, leads to cytoskeletal and electrical abnormalities in a mouse model ofcardiomyopathy. Circ Arrhythm Electrophysiol, 3(6), 646-56.8. Lezin G, Kuehn MR, Brunelli L. (01/01/2011). Hofmeister series salts enhance purification ofplasmid DNA by non-ionic detergents. Biotechnol Bioeng, 108(8), 1872-82.9. Lezin G, Kosaka, Y, Yost HJ, Kuehn MR, Brunelli L. (06/01/2011). A one-step miniprep for theisolation of plasmid DNA and lambda phage particles. PLoS ONE, 6(8), e23457.10. Xi Y, Ai T, De Lange E, Li Z, Wu G, Brunelli L, Kyle WB, Cheng J, Ackerman MJ, Kimura A,Weiss JN, Qu Z, Kim JJ, Faulkner G, and Vatta M. (01/01/2012). Loss-of-function of hNav1.5 byZASP1-D117N is associated with intraventricular conduction disturbances in left ventricularnoncompaction. Circ Arrhythm Electrophysiol, 5, 1017-1026.11. Kosaka Y, Cieslik KA, Li L, Lezin G, Maguire CT, Saijoh Y, Toyo-oka K, Gambello MJ, Vatta M,Wynshaw-Boris A, Baldini A, Yost HJ, Brunelli L. (02/01/2012). 14-3-3ε plays a role in cardiacventricular compaction by regulating the cardiomyocyte cell cycle. Mol Cell Biol, 32(24), 5089-102.12. Arrington CB, Bleyl SB, Matsunami N, Bowles NE, Leppert TI, Demarest BL, Osborne K, YoderBA, Byrne JL, Schiffman JD, Null DM, DiGeronimo R, Rollins M, Faix R, Comstock J, Camp NJ,Leppert MF, Yost HJ, Brunelli L. (12/01/2012). A family-based paradigm to identify candidatechromosomal regions for isolated congenital diaphragmatic hernia. Am J MedGenet, 158A(12), 3137-47.13. Chang B, Gorbea C, Lezin G, Li L, Shan L, Sakai N, Kogaki S, Otomo T, Okinaga T, Hamaoka A,Brunelli, Page 5

Yu X, Hata Y, Nishida N, Yost HJ, Bowles NE, Brunelli L, Ichida F. (02/01/2013). 14-3-3ε GeneVariants in a Japanese Patient with Left Ventricular Noncompaction and Hypoplasia of the CorpusCallosum. (BL co-corresponding author). Gene, 515(1), 173-80.14. Arrington CB, Bleyl SB, Brunelli L, Bowles NE. (05/01/2013). Family-based studies to identifygenetic variants that cause congenital heart defects. Future Cardiol, 9(4), 507-18.15. Lezin G, Bressloff PC, Kumar A, Kosaka Y, Yost HJ, Kuehn MR, Brunelli L. (05/05/2013). Highefficiencyselection-free BAC recombineering using a computational model of the founder principle,Submitted. Nat Commun.16. Brunelli L, Crezee K, Null DM, Yoder BA. (05/15/2013). Heated humidified high flow nasalcannula in congenital diaphragmatic hernia. Submitted. Pediatr Pulmonol.RECENTLY PUBLISHED ABSTRACTS (Last 3 Years)1. Brunelli L, Kuehn MR, Lezin G. (03/17/2011). A one-solution, one-tube plasmid DNA miniprep[Abstract]. FASEB J, 25(540.1).2. Brunelli L, Kuehn MR, Lezin G. (03/17/2011). Hofmeister series salts enhance purification ofplasmid DNA by non-ionic detergents [Abstract]. FASEB J, 25(540.2).3. Lezin G, Kosaka Y, Yost, HJ, Kuehn MR, Brunelli L. (03/29/2012). A novel approach to isolateunselected recombinants facilitates markerless DNA recombineering [Abstract]. FASEB J, 26(584.3).4. Lezin G, Kosaka Y, Yost, HJ, Kuehn MR, Brunelli L. (03/29/2012). Differentiating frequency ofrecombination from frequency of recombinants [Abstract]. FASEB J, 26(584.4).UNPUBLISHED POSTER PRESENTATIONS1. Brunelli L, Liou JY, Chen HP, Wu KK. (2005). Cloning and characterization of the 14-3-3εpromoter: identification of the core region required for PGI2-induced upregulation. Poster sessionpresented at PAS 2005.2. Cieslik KA, Michalik L, Wahli W, Yost HJ, Brunelli L. (01/01/2009). A Transcriptional PathwayRegulating Ventricular Morphogenesis. Poster session presented at Weinstein CardiovascularDevelopment Conference, San Francisco CA.3. Li L, Cieslik KA, Chang B, Toyo-oka K, Gorbea C, Lezin G, Bowles N, Ichida F, Wynshaw-Boris A,Baldini A, Yost HY, Brunelli L. (01/01/2010). 14-3-3 epsilon is essential for heart morphogenesisand is involved in human congenital cardiomyopathy. Poster session presented at WeinsteinCardiovascular Development Conference, Amsterdam, The Netherlands.4. Lezin G, Kumar A, Kosaka Y, Yost HJ, Kuehn MR, Brunelli L. (01/01/2012). Does gene targetingfor the generation of mouse conditional alleles still require selection markers and Gatewaytechnology? Poster session presented at Mouse Molecular Genetics, Pacific Grove, CA.5. Brunelli L, Chang B, Bowles NE, Sakai N, Hamaoka A, Yost HJ, Ichida F. (01/01/2012). Thescaffold adapter protein 14-3-3ε is involved in human left ventricular noncompaction. Poster sessionpresented at Pediatric Academic Societies.ORAL PRESENTATIONSMeeting Presentations (Not Published Abstracts and Not Unpublished Posters)International1994 Brunelli L. Catalase can scavenge nitric oxide. 9th European Congress ofAnesthesiology, Jerusalem, Israel1994 Brunelli L. Use of nitric oxide in full term and preterm infants. International Meetingon Nitric Oxide, 1997. Genoa, Italy1997 Brunelli L. Nitric oxide and free radicals in neonatal and pediatric intensive care.Symposium of Anesthesia and Intensive Care (SMART), Milan, ItalyBrunelli, Page 6

1997 Brunelli L. Oxidants as modulators of cellular activity. 33 rd Congress of theInternational Union of Physiological Sciences (IUPS), St. Petersburg, RussiaNational2009 Cieslik KA, Michalik L, Wahli W, Yost HJ, Brunelli L. A Transcriptional PathwayRegulating Ventricular Morphogenesis. Weinstein Cardiovascular DevelopmentConference, San Francisco, CA2010 14-3-3ε is essential for heart morphogenesis. PAS 2010, Platform presentation2011 Kosaka Y, Lezin G, Michalik L, Wahli W, Yost HJ, Brunelli L. A TranscriptionalPathway Regulating Ventricular Morphogenesis. Pediatric Academic Societies 2011,Denver, CO. Platform presentation2011 Kosaka Y, Lezin G, Gittenberger-de Groot AC, Saijoh Y, Frank D, Wisse LJ, ToyookaK, Wynshaw-Boris A, Yost HJ, Brunelli L. Deletion of 14-3-3ε in Mice Leads toTricuspid Abnormalities and Gerbode Defect. Pediatric Academic Societies 2011,Denver, CO. Platform presentation2012 Arrington CB, Bleyl SB, Demarest BL, Osborne KA, Bowles NE, Yost HJ, BrunelliL. A novel family-based genetic approach efficiently identifies candidate genes forcongenital diaphragmatic hernia. Pediatric Academic Societies 2012, Boston, MA.Platform presentation2012 Ichida F, Chang B, Gorbea C, Lezin G, Sakai N, Kogaki S, Otomo T, Okinaga T,Hata Y, Nishida N, Yost HJ, Bowles NE, Brunelli L.14-3-3ε Gene variants in leftventricular noncompaction and hypoplasia of the corpus callosum. American HeartAssociation Scientific Sessions 2012, Los Angeles, CA. Platform presentationInvited/Visiting Professor PresentationsInternational2008 The transcriptional regulation of 14-3-3ε in human endothelial cells. GianninaGaslini Children’s Hospital, Genoa, Italy2013 The role of 14-3-3ε in left ventricular non-compaction. Developmental BiologyInstitute of Marseilles (IBDML), Aix-Marseille University, France2013 The role of 14-3-3ε in left ventricular non-compaction. Clinic for CardiovascularDiseases, the University of Genoa Medical School, Genoa, ItalyGrand Rounds Presentations2009 The role of 14-3-3ε in heart development. Pediatric Grand Rounds, University ofOklahoma, Oklahoma City, OK2011 14-3-3ε and PPARδ in heart development. Pediatric Grand Rounds, University ofOklahoma, Oklahoma City, OK2011 Meconium aspiration syndrome. Neonatology Grand Rounds, GeorgetownUniversity, Washington DCBrunelli, Page 7