Interleukin-33 Induces Expression of Adhesion Molecules and ...

Supplemental Table II. IL-1 receptor antagonist does not counteract IL-33-induced
ICAM-1, VCAM-1, E-selectin and MCP-1 mRNA expressions in HUVEC.
IL-33 IL-33 + IL-1Ra IL-1β IL-1β+ IL-1Ra
ICAM-1 55.8±8.7 54.1±5.7 20.3±1.0 0.5±0.1
VCAM-1 72.6±2.2 76.8±3.1 33.2±5.7 0.3±0.1
E-selectin 1032.1±99.2 1021.8±17.1 343.3±5.7 0.4±0.1
MCP-1 73.3±0.7 65.2±9.2 20.4±2.2 1.2±0.1
Confluent monolayers of HUVEC were incubated for 6 h in the absence or presence of IL-33
(100 ng/mL) or IL-1β (200 U/mL) with or without IL-1Ra (10 µg/mL). mRNA was prepared
and RealTime-PCR with primers specific for ICAM-1, VCAM-1, E-selectin and MCP-1 was
performed as described in “Methods”. Adhesion molecules and MCP-1 mRNA levels were
normalized according to the respective GAPDH mRNA level. Each experiment was
performed in triplicates. Values are given as x-fold of control, which was set as 1 and
represent mean ± SD of 2 different experiments. The overall ANOVA for each tested
molecule remained significant after correction for 4 comparisons.
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