20100409 抗体精製プロセスのダウンストリーム工程 ... - DKSHジャパン

Prathima Acharya, PhD., Diosynth BiotechnologyDifferential Scanning Calorimetry = DSCDSCProtein ADSCDiosynth BiotechnologyProtein A 1000pHpH” ” ” ”

UF/DF Hold Step DSCProtein ADSCDSCPD-10GE Healthcare, Piscataway, New Jersey1 mg/mlVP-DSC (MicroCal, Northampton,Massachusetts) 5901/minMicroCal Origin22Protein AProteinAProtein AIgFcProtein ApHpH7Protein ApHpH3.5Protein A100Protein A17,00012,000100Protein ApHpHProtein A120gpHProtein ApHpHProtein ADSCDSCpHpHDSCT m Protein AProtein AXDiosynth BiotechnologyXXProtein A12gX

pHpH7.0pH7.3pH3.0pH5.0DSCDSC1XpH7.0pH7.3pH3.5T m XpHpHonset pH7.0 pH7.3 pH3.5pH5.0XT m pH7.0onsetpH7.0pHpHXpHDSCT m 2XpH3.5T m T m onsetpH3.53DSCProtein AXProtein ADSCProtein AXProtein AProtein A1X15g2g/L7.5Protein A19.5L175,000DSCProtein A2.6L23,400Protein AXUF/DF100L0.5g/LProtein A1438,0001,500L570,0001T m onsetDSC27XDSCDSC22T m Citrate + Mannitol PH3.53XDSCDSC

DSCDSCPurification Development Group Rochelle Bazemore,Sorina Morar, Sue Cook Jessica WeavernPathima Acharya, PhD Research Triangle Park, NCDiosynth Biotechnology Analytical and FormulationDevelopment Group Senior Scientist100,000DKSH Using Differential ScanningCalorimetry to Make Downstream Purfication Processing More Economically Viable : ACase Study