BDâ„¢ P100 Blood Collection Kit

BD P100 v1.1 Immediately Stabilizes Plasma Proteins
BD P100 v1.1 versus EDTA Plasma
“Time 0” Gel Plot Summary of Eight Subjects (m/z 1000-2400)
BD P100 v1.1 versus EDTA Plasma
“Time 0” Zoom Plot (m/z 1700-2050)
Figure 1. Preservation of plasma proteins with BD P100
v1.1 Blood Collection Kit. Blood was drawn directly from
eight subjects into P100 and EDTA evacuated tubes. Plasma
was processed within 10 minutes of blood collection by
centrifugation at 2,500 x g for 15 minutes at room temperature.
After blood collection, plasma was transferred into microtubes
and prepared for mass spectrometry analysis or stored at –80ºC
for future analysis. Peptides were isolated from the plasma
samples and analyzed by MALDI-TOF MS. The mass spectra
were collected in the mass range from 800 to 3200 m/z. Areas
within the red rectangles demonstrate enhanced peptide
stabilization in the P100 samples versus EDTA plasma samples.
A greater degree of proteolytic degradation is also more
apparent in the EDTA plasma samples, even though samples
were processed immediately upon blood collection (“Time 0”),
as shown in the yellow rectangle areas.
Figure 2. Enhanced stability of peptides observed in
plasma samples collected with BD P100 v1.1 Blood
Collection Kit. Regions with red arrows on this
MALDI-TOF MS zoom plot (m/z 1700-2050) of two
subjects demonstrates the ability of P100 v1.1 to
stabilize plasma peptides more effectively in the
presence of protease inhibitors. These peaks are absent
in the same regions on the EDTA zoom plots for both
subjects. The immediate mixing of blood with the onboard
protease inhibitors in BD P100 tubes enhances
the recovery of plasma proteins for proteomics studies.