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Appendix F: Troubleshooting and solutions toPipetting ErrorsResearchers can often experience considerable deviations from preferred pipettingvolumes. These deviations can be caused by cross-contamination or liquid-handlingerrors, as well as peripheral factors. Molecular BioProducts prides itself on the patentedART tips that provide 100% guaranteed protection against cross-contamination, but ifyou find that you are still experiencing issues, below we have provided the causes andsolutions to some commonly occurring pipetting errors.Error Cause SolutionPipet drips or leaks Tip is loose; tip does not fit correctly Use original tip; press on tightlyDispensing button does not movesmoothlyNose cone is scratchedSeal of nose cone is leakingPiston is contaminated by reagent depositsPiston is damagedPiston is seal damagedPiston scrapes and is contaminatedSeal is swollen by reagent vaporsPiston is visibly damaged or coated withinsoluble depositReplace nose coneReplace nose coneClean and lubricate piston; replace sealReplace piston and piston sealReplace seal and lubricate pistonClean and lubricate pistonOpen Pipet and ventilate;lubricate piston if necessaryReplace piston and piston sealInaccurate volume Deviating pipetting conditions See table belowPipet is leakingPipet is misadjustedCheck for leaks then proceed as aboveRecalibrate as describedCauses of inaccurate volume Maximum error (inaccuracy) SolutionPipet is held at an angle + 0.5% Hold Pipet straightPipet tip does not fit correctly > 0.4% Use original ART or Hydrologix tipsDensity of medium(eg, r=1.1 g/cm 3 )Temperature differences(eg, Pipet 22 o C, sample 4 o C)Geographical location(eg, 1,000 m above sea level)- 0.4% Adjust Pipet- 5.4% Align Pipet and medium- 0.4% Adjust PipetReference Information1261.800.995.2787 1.800.453.7551 info@mbpinc.com