NEW - Gorea plus doo

Catalog2007-2009Cell CultureCryogenicsImmuno AssaysART/IVFStorage & HandlingProteomics/GenomicsProducts forAssistedReproductiveTechnologiesBio Production Plates Custom Serviceswww.nuncbrand.com

Always with updated information - www.nuncbrand.comNew ProductsCell CultureStorage & HandlingNew ProductsEasYFlasks Page 11Poly-D-Lysine Coated FlasksLiveCell Array Page 28Microscope SlidesCentrifuge Tubes 10/11 ml Page 91Non-pyrogenic and MEA-testedCryogenicsOptiCell Page 18-19Cell Culture SystemCryobank Vials and Bank-It Page 50-53Tube SystemSupersorp Absorbant Material Page 92Transport ContainersImmuno AssayMultidishes Page 204 and 8 Well NunclonΔ SurfaceImmuno LockWell Page 59Fluoronunc/Luminunc ModulesUniversal Container with Spoon Page 93in Compact Transport ContainerART/IVFCell Culture Inserts Page 22-23CE Marked Dishes for IVF Page 84Sputum Container Page 942

www.nuncbrand.com - Always with updated informationNEWProteomics & GenomicsPlatesNew ProductsProPur Protein Purification Kits Page 104-10596 DeepWell Plates 1.0 ml Polystrene Page 15996 Well Caps for DeepWell Plates1536 Well High Base Plates Page 173Bio ProductionCell Factory EasyFill Page 130NunclonΔ Surface384 ShallowWell Page 164Standard Height Plates, PSSealing Tapes Page 178PC Connector Page 130Cell Factory Accessory384 Well Optical Bottom Plates Page 168Polystyrene/Coverglass BaseIn Vitro Roller Bottles Page 138PDL-coated384 ShallowWell Page 170Standard Height PlatesAll new productscan also be found at:www.nuncbrand.com3

Always with updated information - www.nuncbrand.comTable of ContentsTable of contentsNew Products.....................................................2Introduction.......................................................6Cell CultureCell Culture Introduction.................................9Cell Culture Range...........................................10EasYFlasks......................................................11Flasks Nunclon∆ Surface............................12Flasks Non-treated..........................................13SoLo Flasks NunclonΔ Surface..................14Caps for Flasks NunclonΔ...........................14TripleFlasks Nunclon∆ Surface..................15OmniTrays NunclonΔ Surface...................16Cell Scrapers.....................................................17Serological Pipettes........................................17CryoQuot....................................................... 40CryoTubes Internal Thread, Barcoded... 40Custom Barcoded CryoTubes....................41Barcode Scanners...........................................41CryoTubes External Thread...................... 42CryoRacks for CryoTubes67 cm Aluminum............................................ 44-20˚C Labtop Cooler...................................... 44MAX-100 CryoStore Boxes......................... 45Storage Boxes for CryoTubes.................... 46Miniboxes for CryoTubes............................47CryoTube Rack..............................................47Accessories for Nunc CryoTubes.............. 48CryoFlex......................................................... 49Cryobank vials and Bank-ItTube System..................................................... 50Immobilizer - Glutathione Plates.............75Immuno Washers.........................................76MicroWell MiniTrays................................... 77Immuno TSP................................................. 78Immuno OmniTray........................................ 78Immuno Stick............................................... 79Immuno Tubes MiniSorp........................ 80Immuno Tubes PolySorpand MaxiSorp................................................81ART/IVFART/IVF Introduction..................................... 831-cell stage mouse embryotoxicity tested.................................................. 84CE Marked Dishes for IVF ............................. 84OptiCell Cell Culture System......................18Multidishes NunclonΔ Surface................. 20Multidishes Non-treated...............................21Cell Culture Inserts ........................................ 22Dishes NunclonΔ Surface.......................... 26Thermanox Coverslips............................... 27Microscope Slides........................................... 27LiveCell Array Microscope Slides............. 28Tubes NunclonΔ Surface........................... 29MiniTrays NunclonΔ................................... 29Lab-Tek Chamber Slide System............ 30Lab-Tek II Chamber Slide System..........31Lab-Tek II - CC² ChamberSlide System...................................................31Lab-Tek Chambered Coverglass.............. 32Lab-Tek II Chambered Coverglass.......... 32Comparison of Lab-Tekand Lab-Tek II............................................... 33Flask on Slide................................................... 34Example of low volume usage.....................51Cryobank Vials and Bank-ItAccessories....................................................... 53Immuno AssayImmuno Assay Introduction....................... 55Immuno Surfaces........................................... 56About Immuno Assay Products ................. 58Immuno LockWell Fluoronunc/Luminunc Modules......................................59Immuno 96 Microwell Plates................... 60Immuno Modules........................................... 62Immuno LockWell Modules .................. 64Immuno BreakApart Modules.............. 66Color Coded Modules.....................................67Fluoronunc/LuminuncModules/Plates............................................... 68Immobilizer - Amino Modules/Plates.... 70CovaLink NH Modules............................... 71Storage & HandlingStorage & Handling Introduction.............. 87Centrifuge Tubes 15 ml................................. 88Centrifuge Tubes 50/200 ml........................ 89EZ Flip Conical Centrifuge Tubes............. 90Centrifuge Tubes 10/11 ml.............................91Urine Sample Kits........................................... 92Sampling and Transport System................ 93Sputum Containers........................................ 94Stor-It Tubes.................................................. 95Storage Vials - Rack and Box....................... 96Containers........................................................ 97Disposable Tubes........................................... 98Movettes......................................................... 100Mortar Kit....................................................... 100Proteomics &GenomicsCryogenicsCryogenics Introduction...............................37Immobilizer - StreptavidinModules/Plates............................................... 72Streptavidin Passively CoatedModules/Plates............................................... 73Proteomics & Genomics Introduction.... 103ProPur Kits................................................... 104Nucleolink Plates, Modules and Strips... 1064CryoTubes Internal Thread....................... 38Immobilizer - Nickel-Chelate Plates........74TopYield Strips............................................ 108

www.nuncbrand.com - Always with updated informationPolypropylene Modules.............................. 10996 Well Amplification Plates......................110384 Well Amplification Plates....................111PCR Compatibility Chart.............................112Amplification Tubes and Strips .................114Amplification WorkstationTube Tray and Holder...................................115Amplification Labtop Cooler...................116MicroArray Slides MaxiSorpBlack and Clear Polymer.............................117MicroArray Slides NucleoLinkBlack and Clear Polymer.............................118MicroArray mSeries LifterSlip..................119Glass MicroArray Slides.............................. 120Replication System...................................... 122OmniTray....................................................... 123Bio-Assay Dishes.......................................... 124Rectangular Dishes 1, 4 and 8 Well ......... 125Petri Dishes.................................................... 126Loops and Needles....................................... 127Bio ProductionBio Production Introduction..................... 129Cell Factories EasyFill NunclonΔSurface............................................................ 130Cell Factories NunclonΔ Surface............131Cell Factories for Active GassingNunclonΔ Surface..................................... 132Nunc Cell Factory Instructions.................. 133Cell Factories Accessories........................... 134Cell Factory Flexible SystemCustom Capabilities.................................... 135Cell Factory Hand Manipulator CF40..... 136Automatic Cell FactoryManipulator ACFM...................................... 136Nunc CO₂ Incubator......................................137In Vitro Roller Bottles................................... 1382D MicroHex MicrocarriersNunclon∆ Surface...................................... 139PlatesPlates Introduction.......................................141Overview of Nunc Plates............................ 142Application Information............................ 143F96 MicroWell Plates Polystyrene Clear... 145F96 MicroWell Plates PolystyreneBlack and White............................................ 147Immobilizer F96 MicroWellPlates Polystyrene Black and White........ 148C96 MicroWell Plates Polystyrene......... 149U96 MicroWell PlatesPolystyrene Clear.......................................... 150V96 MicroWell PlatesPolystyrene Clear ..........................................15196 Well Optical Bottom PlatesPolymer Base................................................. 15296 Well Optical Bottom PlatesPolystyrene/Coverglass.............................. 153U96 MicroWell Plates Polypropylene... 154V96 MicroWell Plates Polypropylene... 155U96 DeepWell Plates1.3 ml & 2.0 ml Polypropylene................... 15796 DeepWell Plates1.0 ml Polystyrene........................................ 15896 Filter Plates 1.3 ml................................... 159Immobolizer 384 Well PlatesPolystyrene.................................................... 160384 ShallowWell StandardHeight Base Polystyrene............................. 162384 ShallowWell Plates Polystyrene........ 163384 Well Optical Bottom PlatesPolystyrene/Polymer Base......................... 164384 Well Optical Bottom PlatesPolystyrene/Coverglass Base.................... 165384 Well Plates Polypropylene.................. 166384 ShallowWell StandardHeight Plates Polypropylene..................... 167384 DeepWell Plates Polypropylene........ 1681536 Well Plates Polystyrene..................... 1691536 Well High Base Plates Polystyrene... 170Well Cap Mats............................................... 171Microplate Storage Racks.......................... 172Overview of Nunc Lids.................................174Nunc Lid Guide..............................................175Sealing Tapes.................................................176Custom ServicesCustom Services Introduction................... 179Nunc Custom Coatingof MicroWell Plates................................... 180Customized Products.................................. 181Custom Molding........................................... 181Barcoded Products...................................... 182AdditionalInformationAdditional Information.............................. 184Physical Properties of Nunc BrandProducts.......................................................... 185Well and Tube Geometry............................ 186Chemical Resistance ChartNunc Labware............................................... 188Chemical Resistance Thermanox......... 194Chemical Resistance for Nunc............... 194Cell Culture Thermanox Coverslips...... 194Bulletins.......................................................... 195Tech Notes...................................................... 195Product Reference List................................ 197Keywords........................................................ 202Notes............................................................... 205Table of contents5

Products forTechnologiesAlways with updated information - www.nuncbrand.comIntroductionIntroductionWe are pleased to introduce the2007 – 2008 Nunc brand catalogIn this catalog you will find a wealth of products designedto enhance laboratory performance and productivity. TheNunc team is proud of our continued commitment toproviding world-class technical support for our products.This includes application and product selection support.In November of 2006 we became a part of Thermo FisherScientific. Through this exciting merger we will be able toexpand our solution–based support to our customers bycombining the best mix of brands and products.Our new catalog is heavily supported by our websitewww.nuncbrand.com, where you always can find latestnews and products, as well as technical and scientificresearch material to support your work.We look forward to working with you to achieve yourresearch and production goals and welcome yourrecommendations on how we can serve you better thanbefore.Thank you,Steve SilvermanVice President/General ManagerThermo Fisher Scientific, Laboratory Consumables DivisionCell Culture Cryogenics Immuno AssaysART/IVFStorage & HandlingProteomics/GenomicsART/IVFA sistedReproductiveDrug DiscoveryDiagnostics (OEM)Nunc creates high quality labware for use in biotechnology, pharmaceutical andresearch laboratories, as well as in the production of vaccines and diagnostic kits.... And by the way! We also work in close cooperation with Nalgene6

www.nuncbrand.com - Always with updated informationNunc brand products– a part of Thermo Fisher ScientificNunc is a brand within Thermo Fisher Scientific, the worldleader in serving science. Thermo Fisher Scientific enablescustomers to make the world healthier, cleaner and safer byproviding analytical instruments, equipment, reagents andconsumables, software and services for research, analysis,discovery and diagnostics. With annual sales of more than$9 billion, Thermo Fisher Scientific has 30,000 employeesand serves more than 350,000 customers in pharmaceuticaland biotech companies, hospitals and clinical diagnosticlabs, universities, research institutions and governmentagencies, as well as environmental, industrial quality andprocess control settings.Nunc products quality assuranceQuality involves all aspects of our organization fromproduct development, choice of raw materials, throughproduction to customer service. It is no accident that ourquality control and assurance department is the largest inthe company.Nunc A/S is certified* to ISO 9001:2000 and ISO13485:2003 and designed to comply with US current GoodManufacturing Practice for Medical Devices.Nunc A/S is certified* to Environmental ManagementSystem Standard ISO 14001:2004. It is our policy to act ina way, that continuously reduces environmental impact ingeneral, and focus especially on reducing waste-production,consumption of energy, water, materials and hazardoussubstances, while increasing the amounts of waste recycled.IntroductionAbout the CatalogThe raw materials used to manufacture the products in thecatalog are chosen for their suitability for the individualproducts. Each has the optimal characteristics for theintended applications. There are minimal additions made tothe purest possible materials. We do not use releasing agentsor similar additives in our injection molding processes.* Valid for products manufactured in Denmark.Further information about our certifications canbe found at our website.Where applicable, we have quoted recommended workingvolumes. These are only meant as guidelines, as we areaware that our customers will decide for themselveswhat volumes to use in their application of our products.We have chosen to quote those values, which are used inour own laboratories.Necessary details including dimensions of wells, whichmay, for example, be required for the optimization ofassays, are appended in order to allow the best possible useof the products for as many applications as possible.All the information is also available on our website,www.nuncbrand.com7

Always with updated information - www.nuncbrand.comCell Culture8

www.nuncbrand.com - Always with updated informationCell Culture IntroductionThe products in this section are used in all kinds of basic and advancedcell culture research. The products are flasks, dishes, multidishes,slides and other products. Plates are found in the separateplate section of this catalog and products for large scale cell cultureare found in the bio production section.Most of Nunc brand’s cell culturevessels are made of highly characterized,virgin polystyrene with a surfacemodified for adherent cell culture. TheNunclon trademark molded in theproduct indicates the item is manufacturedfrom this special cell culturegrade polystyrene and has undergonethe NunclonΔ surface modificationto facilitate cell attachment. TheNunclon treatment is limited to theintended growth area. Areas such asnecks of flasks, sides of dishes andplates are not treated. This prevents cellattachment and growth in undesiredareas.The NunclonΔ polystyrene surface iscertified for monolayer formation withtwo different cell lines and a primarycell culture. Cloning efficiency isperformed with a specially selected cellline that has a high sensitivity to thepresence of toxic compounds. The celltypes used in the NunclonΔ certificationprocedure are as listed:• PCE: Primary Chick Embryo cells.These cells are used to test forthe NunclonΔ treated surface insupport of primary cell growth.• F2002: This line was derived fromembryonic lung tissue of a humanmale. It is a diploid fibroblast-likecell line initially developed for usein vaccine development. It is used totest for monolayer formation.• HEL: This line was derived fromHuman Embryonic Lung fibroblasts.It is used to test for monolayerformation.• V79-4 (ATCC CCL93): This cell linewas derived from the lung tissue ofa male Chinese hamster. These cellsare used to test for cloning efficiency.• L929 (ATCC CCL 1): This fibroblast-likecell line was derived froma cloned strain. The parent L strainwas derived from normal subcutaneosareolar and adipose tissue of amale C3H/An mouse. It is used fortesting monolayer formation.NEWNEWNEWNEWContentsCell Culture Range.................................10EasYFlasks.............................................11Flasks Nunclon∆ Surface...................12Flasks Non-treated.................................13SoLo Flasks NunclonΔ Surface.........14Caps for Flasks NunclonΔ..................14TripleFlasks Nunclon∆ Surface.........15OmniTrays NunclonΔ Surface..........16Cell Scrapers............................................17Serological Pipettes...............................17OptiCell Cell Culture System.............18Multidishes NunclonΔ Surface.........20Multidishes Non-treated......................21Cell Culture Inserts ................................22Dishes NunclonΔ Surface..................26Thermanox Coverslips.......................27Microscope Slides...................................27LiveCell Array Microscope Slides.....28Tubes NunclonΔ Surface....................29MiniTrays NunclonΔ...........................29Lab-Tek Chamber Slide System.....30Lab-Tek II Chamber Slide System...31Lab-Tek II - CC² ChamberSlide System..........................................31Lab-Tek Chambered Coverglass......32Lab-Tek II Chambered Coverglass...32Cell CultureThe raw material has successfullypassed the USP biological reactivityclass VI test -50°C (7 days implant).We observe the guidelines for sterilityby irradiation as set out in the ISOdocumentation.Comparison of Lab-Tekand Lab-Tek II.......................................33Flask on Slide...........................................34CertificateNunclon∆ SurfaceThis certificate is your guarantee that representative sterilized samples ofthis product have been validated according to the following tests.Monolayer formation:Two different cell lines and one primary cell culture.Cloning efficiency:Specially selected cell line with high sensitivity to toxic components.USP VI:Materials have successfully passed the USP biological reactivity classVI-50° C test (7 days implant).Sterility:Sterility is obtained through irradiation according to ISO 11137(sterilization of health care products - requirements for validation and routinecontrol - radiation sterilization). Closed systems are based onfluid path method.Non-pyrogenic:This batch is tested according to the principles of the LAL-test described inthe FDA guidelines and certified non-pyrogenic with a documented endotoxinlevel of less than 20 Endotoxin units/device (0.5 Endotoxin units/ml)as stated in the USP.Pyrogens and endotoxins are biologicalcompounds, which are heat stableand have a detrimental effect on cellgrowth and proliferation. As they arenot destroyed by radiation a separatetest is necessary to indicate theirabsence.Red colour of this dot indicates exposure to irradiation.For information on tests used please refer to www.nuncbrand.comThe Management System of Nunc A/S,Roskilde, Denmark, has been approved byLloyd’s Register Quality Assurance Limitedto the Quality Management SystemStandard ISO 9001:2000, ISO 13485:2003 andEnvironmental Management System IS0 14001:19969

Always with updated information - www.nuncbrand.comCell Culture Range0.0130.0840.330.400.80Culture area cm2 (log)1.101.801.903.504.205.508.609.009.409.6010.021.525.056.717517550050063212646320Cell Culture100,00010,0003.1 x 10-70.0200.02075.01001451852252528252801,0001101000.10.010LiveCell ArrayMini Trays1536 Well Plates384 ShallowWell Plates384 Plates96 MicroWell PlatesLabTek 16LabTek 8Multidish 48LabTek 4Multidish 4 & 24Multidish 12LabTek 2CC TubeMultidish 8Slide FlaskLabTek 1Multidish 6FlasketteDishEasYFlaskDishEasYFlaskOptiCellDishFlaskEasYFlaskSoLo FlaskEasYFlaskTripleFlaskDishCell Factory 1/EasyFill 1Cell Factory 2/EasyFill 2Cell Factory 4/EasyFill 4Cell Factory 10/EasyFill 10Cell Factory 4010

www.nuncbrand.com - Always with updated informationEasYFlasks• NunclonΔ certified or coated withPoly-D-Lysine or Collagen I (surfaceensures cell attachment, facilitatesgrowth)• Allow full access to the growth surface• Ergonomic closure, open or close in 1/3turn• “Y” - mark allows visual verification ofvent position, even when stacked inincubators• Volume graduations on both sides• Vent/close and filter caps available• Extra caps in every cartonCell CultureFilter caps for continuous ventingThe caps are fitted with a hydro phobicfilter to ensure consistent gas exchangeNunc EasYFlasks NunclonΔPolystyrene. Sterile“Y” mark indicating “vent” or “closed”Any of the legs pointingvertically upwards vertically downwardsAny of the legs pointingindicates that the cap indicates that the flaskis in vent position is closedLiterature:Bulletin Nos: 2, 3, 5, 13Tech Note Nos: 2, 3, 25 Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksCat. No. 156340 156367 156472 156499 159920* 159910* 159933* 159934*Culture area, cm² 25 25 75 75 175 175 225 225Neck style Angled Angled Angled Angled Angled Angled Angled AngledClosure Vent/close Filter Vent/close Filter Vent/close Filter Vent/close FilterCap material HDPE HDPE HDPE HDPE HDPE HDPE HDPE HDPESuggested working 7 7 25 25 55 55 70 70volume, mlUnits per pack/case 10/200 10/200 5/100 5/100 5/30 5/30 5/30 5/30HDPE=High Density Polyethylene * Batch No. and Cat. No. printed on each flaskNEWNunc EasYFlasks Poly-D-Lysine and Collagen I coatedFilter Cap. SterileAccessories:Caps for Flasks NunclonΔ Page 14Serological Pipettes Page 17Cell Scrapers Page 17Cat. No. 132703 132704 132705 132706 132707 132708DescriptionPoly-D-LysineCoated 25cm²Poly-D-LysineCoated 75cm²Poly-D-LysineCoated 175cm²Collagen I Coated25cm² EasYFlaskCollagen I Coated75cm² EasYFlaskCollagen I Coated175cm² EasYFlaskEasYFlaskEasYFlaskEasYFlaskUnits per pack/case 10/60 5/30 5/30 10/60 5/30 5/30AccessoriesLoose Caps for Nunc EasyFlasks NunclonΔ. SterileCat. No. 158892 158523 158240 157527 133001 133002Closure Vent/close Filter Vent/close Filter Vent/close FilterUse for flask size, cm² 25 25 75 75 175/225 175/225Units per pack/case 1/100 1/100 1/100 1/100 1/100 1/10011

Always with updated information - www.nuncbrand.comFlasks Nunclon∆ SurfaceCell Culture156502178883• Cell culture flasks with surface areasfrom 25-175 cm²• Filter caps or vent/close caps availablefor good gas exchange• Extra caps included in every carton• Short, wide neck for easy access• Individually leak tested• Excellent optical quality• NunclonΔ certified153732178905• Barcoded 175 cm² with large Code 128152094Literature:Bulletin Nos: 2, 3, 5Tech Note Nos: 2, 3, 25 Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and links136196163371Accessories:Caps for Flasks NunclonΔ Page 14Serological Pipettes Page 17Cell Scrapers Page 17178983Vent/close CapFilter CapNunc Flasks NunclonΔwith Filter CapPolystyrene. SterileCat. No. 136196 178905 178883 178983*Culture area, cm² 25 80 175 175Neck style Angled Straight Straight StraightClosure Filter Filter Filter FilterCap material HDPE HDPE HDPE HDPESuggested working vol., ml 7 30 68 68Units per pack/case 20/160 5/50 4/32 4/32HDPE = High Density Polyethylene*Barcoded with large Code 12812Nunc Flasks NunclonΔwith Vent/Close CapPolystyrene. SterileCat. No. 152094 163371 153732 156502Culture area, cm² 24 25 80 175Neck style Straight Angled Straight StraightClosure Vent/close Vent/close Vent/close Vent/closeCap material HIPS HDPE HDPE HDPESuggested working vol., ml 7 7 30 68Units per pack/case 20/200 20/160 5/50 4/32HIPS = High Impact Polystyrene / HDPE = High Density Polyethylene

www.nuncbrand.com - Always with updated informationFlasks Non-treated• Non-treated, sterile flasks forsuspension cell cultures• White caps for easy identification• Extra cap in every carton• Non-pyrogenicCell CultureAccessories:Caps for Flasks NunclonΔ Page 14Serological Pipettes Page 17Cell Scrapers Page 17Nunc Flasks Non-treatedPolystyrene. SterileCat. No. 169900 156800 159926 144903 132903Flask type EasYFlask EasYFlask EasYFlask SoLoFlask TripleFlaskCulture area, cm² 25 75 175 185 500Total volume, ml 70 260 645 600 800Neck style Angled Angled Angled Angled StraightClosure Filter Filter Filter Filter FilterCap material HDPE HDPE HDPE HDPE HDPESuggested working vol., ml 7 30 55 75 200Units per pack/case 10/200 5/100 5/30 5/50 4/32Caps for Non-treated FlasksWhite. High Density Polyethylene (HDPE).SterileCat. No. 178802 156753 159929Closure Filter Filter FilterUse for flask size, ml 70 260 645Units per pack/case 1/100 1/100 1/100Filter Cap13

www.nuncbrand.com - Always with updated informationTripleFlasks Nunclon∆ Surface• Three parallel growth surfaces providea total culture area of 500 cm²• External dimensions of a 175 cm²standard flask• Ideal for scale-up• Extra caps in every carton• NunclonΔ certified• Barcoded with large Code 128Cell CultureLiterature:Bulletin Nos: 2, 3, 5Tech Note Nos: 2, 3, 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksAccessories:Caps for Flasks NunclonΔ Page 14Serological Pipettes Page 172aNunclon51BOTTOMTOP2TOPBOTTOM3TOP4NunclonBOTTOM1. Prepare homogeneous cell suspension. Pourinto the TripleFlask, tilting flask slightlyto avoid foam or bubbles. Recommendedworking volume is 100-200 ml2. Leave the flask in the upright posi tion for ashort time to allow equilibration of liquidin each compartment2a. The flask may be canted momentarilyaround the connecting channel cornerfor facilitating the equilibra tion of smallvolumes3. Quickly but gently place the flask in theincubation position4. The liquid is equally distributed over thethree growth surfaces5. The flask is emptied in the same way as aconventional flask. To harvest cells add10-15 ml TrypsinNunc TripleFlasks NunclonΔPolystyrene. SterileCat. No. 132867 132913 132920Barcode - - +Culture area, cm² 500 500 500Neck style Straight Straight StraightClosure Vent/close Filter FilterVent/close CapFilter CapCap material HDPE HDPE HDPESuggested working vol., ml 200 200 200Units per pack/case 4/32 4/32 4/32HDPE = High Density Polyethylene15

Always with updated information - www.nuncbrand.comOmniTrays NunclonΔ SurfaceCell Culture140175140156• Single well OmniTray ideal for amultitude of uses• Available both as a standard versionwith notched corners and as arectangular version optimized forcomputerized imaging and spotting• NunclonΔ certified165218Literature:Bulletin Nos: 2, 3Tech Note Nos: Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksAccessories:Lids for 96 and 384 Well Plates Page 17616Nunc OmniTrays NunclonΔExternal dimensions 128 x 86 mmPolystyrene. SterileCat. No. 165218 140156* 140175*Color Clear Clear WhiteSuggested working vol., ml 90 90 90Culture area, cm² 84 84 84Units per pack/case 10/60 10/90 10/90*Enhanced rectangular area ideal for automatic picking and viewing system

www.nuncbrand.com - Always with updated informationCell Scrapers• Available in two lengths• Blade adjustable for optimal applicationflexibility• Non-pyrogenicCell CultureNunc Cell ScrapersSterileCat. No. 179693 179707Use for flask size, cm² 25-80 75-175Total length, cm 23 32Blade offset, mm 7.5 16Blade width, mm 15.5 17.5Units per pack/case 1/250 1/250Serological Pipettes• Accurate, disposable plastic pipettesplugged and sterilized• Sterility assurance level (SAL) of 10-⁶• Bold clear graduations witheasy - to - read scale markings• Color-coded packaging for ease in sortingand selecting the correct size• Individually wrapped inpaper - peelable bags• Convenient extra graduations to fullpipette volume• Printed with a black scale• Non-pyrogenicNunc Serological PipettesPolystyrene. SterileCat. No. 159609 159617 159625 159633 159641 159668Total volume, ml 1 2 5 10 25 50Sub division, ml 0.01 0.01 0.1 0.1 0.2 0.5Extra grad., ml 0.3 0.3 2 3 10 10Tolerance ± 0.02 0.04 0.10 0.20 0.50 0.75Color code Black Black Blue Orange Green PurpleUnits per pack/case 200/1000 125/500 50/200 50/200 50/200 25/10017

Always with updated information - www.nuncbrand.comCell CultureOptiCell Cell Culture SystemNEW• OptiCell is a unique cell cultureformat for growing, monitoring, andtransporting cells• Two parallel gas-permeable, cell culturetreated polystyrene membranesattached to a standard microtiterplate-sized frame--Each side has a growth areaof 50 cm², total 100 cm²--75 µm thick membranes, 2 mm apart• Thin profile design maximizesincubator space and reduces mediumconsumption• Recommended medium/cell fill volume10 ml for OptiCell and 30 ml for OptiCellMAX• Two resealing access ports provideclosed growth environment with sterilefluid path, thereby reducing risk ofcontamination• Barcoded for easy tracking andautomation handling• Applications--Transportation of live cells--Hybridoma antibody production(OptiCell MAX)--Short-term freezing and thawing of cells--Biomagnetic cell separation (OptiMag Kit)--Cell imaging and staining--TranfectionOptiCell is a registered trademarkof BioCrystal, LTD.NEWNunc OptiCell Kits and ChambersCat. No. 155330 155331 155332 155333DescriptionOptiCell 1100 Starter Kit.Contains 20 each of OptiCell 1100,1 rack, 1 knife, 50 tips, manualOptiCell1100ChambersOptiCell1100ChambersOptiCell1100ChambersUnits per case 1 20 100 500NEWNunc OptiCell MAX Kits and ChambersCat. No. 155334 155335 155336DescriptionOptiCell MAX 2100 Starter Kit.Contains 5 each of OptiCell MAX2100, 1 rack, 50 tips, HybridomaOptiCellMAX 2100ChambersOptiCellMAX 2100ChambersAb production protocol/manualUnits per case 1 20 10018

www.nuncbrand.com - Always with updated information155342155338 155344Cell Culture155343155340NEWNunc OptiCell Separation Kit and MagnetCat. No. 155337 155338DescriptionOptiMag Cell Separation Kit.OptiMag MagnetContains 20 each of OptiCell 1100,1 magnet, 50 tips, 1 rack, 2 clips,OptiMag cell separation protocol/manualUnits per case 1 1NEWNunc OptiCell Mailer KitCat. No. 155339 155340DescriptionOptiCell Mailer Kit. Contains 5 each OptiCell Mailerof OptiCell 1100, 50 tips, 5 OptiCellMailers, shipping protocol /manualUnits per case 1 10NEWAccessories for Nunc OptiCell Cell Culture SystemCat. No. 155342 155343 155344DescriptionOptiCell Rack for OptiCell 1100 and OptiCell Insertion TipsOptiCell Knife2100Units per case 1 100 319

www.nuncbrand.com - Always with updated informationMultidishes Non-treated150239• Non-treated, sterile multidishes forsuspension cell cultures• Raised well rims to lower risk of crosscontamination• Excellent optical quality• Non-pyrogenicCell Culture179820144530150787150200Literature:Bulletin Nos: 2, 3, 5Tech Note Nos: 3, 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksAccessories:Serological Pipettes Page 17Nunc Cell Culture Inserts Page 22Multidishes, Non-treatedPolystyrene. With lid. SterileCat. No. 179820 150239 150200 144530 150787Number of wells 4 6 12 24 48Bottom shape Flat Flat Flat Flat FlatSuggested working vol., ml/well 1 3 2 1.0 0.5External dimensions, mm 66 x 66 128 x 86 128 x 86 128 x 86 128 x 86Units per pack/case 4/120 1/75 1/75 1/75 1/7521

Always with updated information - www.nuncbrand.comCell CultureCell Culture InsertsNEW• For multidishes 6, 12, 24• Treated and tested for cell attachment• Easy cultivation of most cell typeswithout matrix coating• Used in a varity of applications,including transport studies, toxicitytests, chemotaxis studies and electronmicroscopy• Non-toxic• Packed in sterile NunclonΔ treatedmultidishes• Packed in resealable bags.• Non-pyrogenic• Tabs for better grip/handlingLiterature:Tech Note No: 13Porous Cell Culture Insertswww.nuncbrand.com/go/ccinsertsSee Pages 195-196 for full reference list with titles and linksAccessories:Multidishes Page 20Application examplesTransport studies:Molecules, including hormones and growth factorsDrug transport across epithelial (Caco-2) and endothelial barriersDrug transport across brain microvascular endothelial cellsChemotaxis studies:Migration of cells, including eosinophils, neutrophils, macrophagesInvasion studies:Tumor invasion and metastasis modelsInvasion inhibitorsExtra cellular matrix effectsCo-cultivation studies:Cell-cell interactionCell-matrix interactionCell-substrate interactionTissue engineering:AngiogenesisDermal/epidermal and epithelial tissue modelsPore size0.4, 3.0 µm3.0, 8.0 µm3.0, 8.0 µm0.4, 3.0 µm0.4, 3.0 µm22

www.nuncbrand.com - Always with updated informationCell CultureNEWNunc Cell Culture InsertsSterileCat. No. 140620 140627 140629 140652 140654 140656Pore size, µm 0.4 3 8 0.4 3 8For use in and packed in Multidish 24 Multidish 24 Multidish 24 Multidish 12 Multidish 12 Multidish 12Pore density, pores/cm²

Always with updated information - www.nuncbrand.comCell Culture InsertsCell Culture• Easy of cultivation of most cell typeswithout matrix coating• Treated for cell culture• Inserts with 0.02 µm Anoporemembrane have maximum clarityfor microscopy, and are non-autofluorescentand highly porous• Polycarbonate membranes in largerpore sizes are adaptable to many usesin cell culture, including transportstudies, toxicity tests, chemotaxisstudies and electron microscopy• Both membrane types are trans parentwhen wet and suitable for phasecontrast and Nomarski Optic SystemsAnopore is a trademark of Whatman Scientific Ltd.Literature:Tech Note No: 13Porous Cell Culture Insertswww.nuncbrand.com/go/ccinsertsSee Pages 195-196 for full reference list with titles and links24Nunc Cell Culture InsertsAnopore membrane.SterileCat. No. 162243 161395 136935 137044 136730Pore size, µm 0.02 0.02 0.2 0.2 0.2Diameter size, mm 10 25 10 25 8 well StripFor use in Multidish 24 Multidish 6 Multidish 24 Multidish 6 MicroWell PlatePore density, pores/cm² 10¹¹ 10¹¹ 10⁹ 10⁹ 10⁹Porosity % 25-30 25-30 50 50 50Thickness, micron 45 45 45 45 45Height above base of well, mm 1 1 1 1 3Culture area, cm² 0.5 4.2 0.5 4.2 0.08Suggested working volume, ml 0.5 1.75 0.5 1.75 60 µlUnits per pack/case 6/48 6/48 6/48 6/48 5/15All Nunc Cell Culture Inserts: Suggested working volume, ml = in addition to normal well working volume.

www.nuncbrand.com - Always with updated informationWhat is the difference between the Anopore and the Polycarbonatemembrane used in the cell culture inserts?Both the membranes are speciallytreated to provide a surface that isexcellent for cell attachment andgrowth. The Anopore membraneis a rigid inorganic membrane thatis highly transparent when wetand highly porous (40-50% surfaceporosity). This membrane providesmaximum clarity for microscopy, isnon-autofluorescent, and highly resistantto most solvents. Some typicalapplications are epithelial polarizationstudies, transport studies, toxicitytesting and electron microscopy. Anapplications manual is available. SeeTechnical articles index to order.The Polycarbonate membrane does nothave the transparent characteristicsof the Anopore membrane. Due tothe larger pore sizes available with thepolycarbonate membrane, it is particularlysuitable for chemotaxis studies.These membranes are easily removedand are ideal for sectioning for transmissionelectron microscopy using aglass knife.Cell CultureNunc Cell Culture InsertsPolycarbonate membrane.SterileCat. No. 137052 137060 137370 137435 137443 137508Pore size, µm 0.4 0.4 3.0 3.0 8.0 8.0Diameter size, mm 10 25 10 25 10 25For use in Multidish 24 Multidish 6 Multidish 24 Multidish 6 Multidish 24 Multidish 6Pore density, pores/cm² 1.5 x 10⁸ 1.5 x 10⁸ 3 x 10⁶ 3 x 10⁶ 10⁵ 10⁵Porosity % 18 18 14 14 5 5Film thickness, micron 20 20 17 17 12 12Height above base of well, mm 1 1 1 1 1 1Culture area, cm² 0.5 4.2 0.5 4.2 0.5 4.2Suggested working volume, ml 0.5 1.75 0.5 1.75 0.5 1.75Units per pack/case 6/48 6/48 6/48 6/48 6/48 6/48All Nunc Cell Culture Inserts: Suggested working volume, ml = in addition to normal well working volume.25

Always with updated information - www.nuncbrand.comDishes NunclonΔ SurfaceCell Culture168381157150150350150679• Large range available, surface treatedfor cell culture• Optically clear and suitable formicroscopy• 60 and 40 mm dishes available withgrids for cloning or determination ofplating efficiency• NunclonΔ certified174888166508169558150326172958Literature:Bulletin Nos: 2, 3, 5Tech Note Nos: 2, 3, 25 Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and links150318174926153066171099150288Accessories:Serological Pipettes Page 17Cell Scrapers Page 17Dishes NunclonΔPolystyrene. With lid. SterileCat. No. 150318 153066 174926 171099* 174888** 150326 150288 150340* ‡ 169558Name 35 x 10 35 x 10 35 x 10 35 x 10 60 x 15 60 x 15 60 x 15 60 x 15 60 x 15Grid size, mm - - 2 x 2 - - - - - 2 x 2Culture area, cm² 8.8 8.8 8.8 8.8 20.8 21.5 21.5 21.5 21.5Airvent - + + + + - + + +Max. external dimensions, 40 x 12 40 x 12 40 x 12 40 x 12 60 x 15 60 x 15 60 x 15 60 x 15 60 x 15mmMax. external diameter 36.2 36.2 36.2 36.2 48 53.5 53.5 53.5 53.5bottom, mmSuggested working 3 3 3 3 5 5 5 5 5volume, mlUnits per pack/case 10/500 10/500 20/500 20/500 20/500 10/400 10/400 10/400 10/400* For suspension culture - not NunclonΔ treated**Made from Permanox‡ Not available Americas26Dishes NunclonΔPolystyrene. With lid. SterileCat. No. 150350* ‡ 150679 † 172958* 168381* 157150 166508Name 100 x 15 100 x 15 100 x 20 150 x 20 150 x 20 -Grid size, mm - - - - - -Culture area, cm² 56.7 56.7 56.7 145 150 500Airvent + + + + + -Max. external dimensions, 92 x 17 100 x 15 92 x 21 144 x 21 147 x 18 245 x 245 x 25mmMax. external diameter 87.2 136 87.1 138.3 136 -bottom, mmSuggested working 12.5 12.5 12.5 35 35 135volume, mlUnits per pack/case 10/150 10/450 10/480 10/80 10/120 4/16* Packed in resealable bags‡ Not available in Americas† Available only in Americas

www.nuncbrand.com - Always with updated informationThermanox Coverslips• 0.2 mm thickness• Resistant to commonly used solvents• Safe and easy to handle• Autofluorescent in therange 380 to 545 nm• Surface treatment on one side foroptimal cell attachment and growthCell CultureLiterature:Bulletin No: 13Tech Note Nos: 33, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksThermanox CoverslipsSterileCat. No. 150067 174969 174977External dimensions, mm 24 x 30 15 dia. 22 dia.Units per pack/case 50/500 50/500 50/500Accessories:Multidishes Page 20Thermanox CoverslipsSterileCat. No. 174950 174985 174942 174934For use in Nunc product24 wellMultidish6 wellMultidish4 wellMultidish8 wellMultidishExternal dimensions, mm 13 dia. 25 dia. 22 x 60 (rect.) 10.5 x 22 (rect.)Units per pack/case 50/500 50/500 50/500 50/500Microscope Slides• Surface treatment ensures optimalconditions for cell attachment andgrowthLiterature:Bulletin No: 2Tech Note Nos: 15, 20, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksNunc Microscope Slides27 x 75 mm. SterileCat. No. 160005 160004Material Permanox PolystyreneUnits per pack/case 20/100 20/10027

Always with updated information - www.nuncbrand.comCell CultureLiveCell Array Microscope SlidesNEW• First slide-based tool for real time studyof individual, living cells (adhering ornon-adhering) within hetero geneouspopu lations• Disposable, sterile microscope slidewith an embedded transparent arrayof picowells• Enables multiple functional assays onliving cells followed by post fixationstudies on the same cells• Staining, rinsing and perfusion do notdisplace cells• Compatible with standard microscopes• Image Analysis Software Software,commercially-available, can designatean address to each cellLiveCell is a licensed trademarkof Molecular Cytomics Inc.NEWNunc LiveCell Array SlidesCat. No. 130504 130506 130508 130510 130505 130507 130509 130511DescriptionSlide with15 µm wellSlide with20 µm wellSlide with100 µm wellSlide with250 µm wellSlide with15 µm wellSlide with20 µm wellSlide with100 µm wellSlide with250 µm wellUnits per pack 1 1 1 1 5 5 5 5NEWNunc LiveCell Array Apoptosis KitsCat. No. 130512 130513 130514 130515DescriptionApoptosis Kit,slides with 15µm wellApoptosis Kit,slides with 20µm wellApoptosis Kit,slides with 100µm wellApoptosis Kit,slides with 250µm wellUnits per pack5 slides plusreagents5 slides plusreagents5 slides plusreagents5 slides plusreagentsNEWNunc LiveCell Array Cell Surface Marker KitsCat. No. 130516 130517 130518 130519DescriptionCell SurfaceMarker Kit,slides with 15µm wellCell SurfaceMarker Kit,slides with 20µm wellCell SurfaceMarker Kit,slides with 100µm wellCell SurfaceMarker Kit,slides with 250µm wellUnits per pack5 slides plusreagents5 slides plusreagents5 slides plusreagents5 slides plusreagents28

www.nuncbrand.com - Always with updated informationTubes NunclonΔ Surface146183156758• Round bottom version with screw orpush-on cap• Flat-sided tube for culture of adherentcells• Flat side allows easy microscopy, acoverslip can be used in the tube• Medium retained in the flat sided tubein horizontal position• Excellent optical quality• NunclonΔ certifiedCell CultureNunc Tubes NunclonΔPolystyrene tubesPolyethylene caps. SterileCat. No. 146183 145470 ‡ 156758Shape Round Round FlatCap type Screw cap Push-on-cap Screw capSuggested working volume, ml 7 7 3Culture area, cm² - - 5.5External dimensions, mm 100 x 14 100 x 13 110 x 16Units per pack/case 100/600 100/600 75/450‡ Not available in Americas145470Literature:Tech Note Nos: Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksAccessories:Serological Pipettes Page 17Coverslips, Cat. No. 174934 Page 27MiniTrays NunclonΔNunc MiniTraysSterile. PolystyreneExternal dimensions 84 x 59 mmCat. No. 163118 136528Number of wells 60 72Well shape Conical ConicalWith lid + +Suggested working vol., µl 8 8Culture area, cm² 0.013 0.013Units per pack/case 10/150 10/15029

Always with updated information - www.nuncbrand.comLab-Tek Chamber Slide SystemCell Culture177410177429177437177445177399• Cells grow on a standardmicroscope slide• No cell transfer needed prior tovisualization/staining• Upper structure can be removed whenculturing is complete178599177380177402• Useful for viral and mycoplasma testing,chromosome studies, toxicity tests andimmunocytology• Broad range of formatsand well numbers• Fits standard equipment• Saves time and reagents177372• Suitable for use with fluorescent labels171080• CE markedMedia Chamber and Gasket RemovalFix and stain. Gasketmay be used as reservoirfor reagent incubation.To detach slide frommedia chamber, gripend of slide with onehand. Gently squeezeboth ends of mediachamber toward thecenter lifting chamberas gasket releasesGasket removal. Inserttip of a thin bladedspatula or similartool under gasket atone corner. Withoutstretching or tearing thegasket, smoothly lift itaway from the slideLiterature:Bulletin No: 13Tech Note Nos: 15, 20, 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksLab-Tek Chamber SlidesCE marked. SterileCat. No. 177372 177410 177380 177429 177399 177437Number of wells 1 1 2 2 4 4Slide material Glass Permanox Glass Permanox Glass PermanoxSuggested working vol. ml 2.5 - 4.5 2.5 - 4.5 1.2 - 2.0 1.2 - 2.0 0.5 - 0.9 0.5 - 0.9Culture area, cm²/well 9.4 9.4 4.2 4.2 1.8 1.8Units per pack/carton/case 8/16/96 8/16/96 8/16/96 8/16/96 8/16/96 8/16/96Lab-Tek Chamber SlidesCE marked. SterileCat. No. 177402 177445 178599Number of wells 8 8 16Slide material Glass Permanox GlassSuggested working vol., ml 0.2 - 0.4 0.2 - 0.4 0.1 - 0.2Culture area, cm²/well 0.8 0.8 0.4Units per pack/carton/case 8/16/96 8/16/96 8/16/96Accessoriesfor 178599Cat. No. 171080DescriptionCoverglassDimensions, mm 22 x 74Units per pack55 g30

www.nuncbrand.com - Always with updated informationLab-Tek II Chamber Slide SystemLab-Tek IIChamber SlideCE marked. SterileCat. No. 154453 154461 154526 154534Number of wells 1 2 4 8Medium chamber Polystyrene Polystyrene Polystyrene PolystyreneSlide material Glass Glass Glass GlassSuggested working vol., ml 2.0 - 4.5 1.0 - 2.0 0.5 - 1.0 0.2 - 0.5Culture area, cm²/well 8.6 4.0 1.7 0.7Units per tray/pack/case 8/16/96 8/16/96 8/16/96 8/16/96• Removable, polystyrene mediumchamber of 1, 2, 4 or 8-wellconfiguration• Non-fluorescent microscope slide, glass(25 x 75 x 1.2 mm) with rounded corners• Biocompatible adhesive• Polystyrene cover• Inert hydrophobic well border printedon slide• Superfrost printed white writing area• Treated for excellent attachment andgrowth of cells• Slide separator included in each pack• CE markedSuperfrost is a registered trademarkof Erie ScientificLiterature:Bulletin No: 13Tech Note Nos: 20, 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksCell CultureLab-Tek II - CC² Chamber Slide System• Chemically coated growth surface onglass slide that mimics polylysine• Provides binding sites optimal forfastidious cells (e.g. neurons)• Growth surface remains stable withoutrefrigeration• Light blue frosted writing area• Slide separator included in each pack• CE markedLab-Tek II - CC²Chamber SlideCE marked. SterileCat. No. 154739 154852 154917 154941Number of wells 1 2 4 8Medium chamber Polystyrene Polystyrene Polystyrene PolystyreneSuggested working vol., ml 2.0 - 4.5 1.0 - 2.0 0.5 - 1.0 0.2 - 0.5Culture area, cm²/well 8.6 4.0 1.7 0.7Units per tray/pack/case 8/16/96 8/16/96 8/16/96 8/16/96Literature:Bulletin: No: 13Tech Note Nos: 20, 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksSlide Separator lifts mediumchamber from slide31

Always with updated information - www.nuncbrand.comLab-Tek Chambered CoverglassCell Culture• Excellent for confocal image analysis• Optimal for high power invertedmicroscopic viewing• Chambered coverglass• Medium chamber is not removable• CE markedLab-TekChambered CoverglassCE marked. Sterile.1.0 Borosilicate coverglass.Cat. No. 155361 155380 155383 155411Number of wells 1 2 4 8Medium chamber Polystyrene Polystyrene Polystyrene PolystyreneSlide material Glass Glass Glass GlassSuggested working vol., ml 2.5 - 4.5 1.2 - 2.0 0.5 - 0.9 0.2 - 0.4Culture area, cm²/well 9.4 4.2 1.8 0.8Units per pack/carton/case 8/16/96 8/16/96 8/16/96 8/16/96Literature:Bulletin No: 13Tech Note Nos: 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksLab-Tek II Chambered Coverglass• Excellent for confocal image analysis• Optimal for high power invertedmicroscopic viewing• Chambered coverglass• Medium chamber is not removable• CE markedLab-Tek IIChambered CoverglassCE marked. Sterile.1.5 Borosilicate coverglass.Cat. No. 155360 155379 155382 155409Number of wells 1 2 4 8Medium chamber Polystyrene Polystyrene Polystyrene PolystyreneSuggested working vol., ml 2.0 - 4.5 1.0 - 2.0 0.5 - 1.0 0.2 - 0.5Culture area, cm²/well 8.6 4.0 1.7 0.7Units per tray/pack/case 8/16/96 8/16/96 8/16/96 8/16/96Literature:Bulletin No: 13Tech Note Nos: 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and links32

www.nuncbrand.com - Always with updated informationComparison of Lab-Tek and Lab-Tek IICell CultureNunc Chamber Slide SystemFeature Lab-Tek Product Lab-Tek II ProductConfigurations 1, 2, 4, 8, 16 well, flask style 1, 2, 4, 8 wellMedium chamber material Proprietary cell culture grade polystyrene Proprietary cell culture grade polystyreneSlide materialsCoverSealantSoda Lime glassPermanox plasticPolystyrene plasticClear polystyrene, no handle, facilitatesmicroscopic viewingNon-toxic medical grade siliconeRemains on slide after detachmentSoda Lime glassClear polystyrene with top handlefor aseptic manipulationsBiocompatible acrylic adhesive - Remains withmedia chamber after detachmentReagent “dam” Created by removable silicone gasket Created by hydrophobic borderComponent separation No separation tools required Requires separation toolsGrowth surfaceFor adherent cell culture:Glass slides - Six stage high purity water washPermanox Plastic - Cell culture treatedPolystyrene - Cell culture treatedBiological testing BHK-21, HEp-2 cells BHK-21, HEp-2 cellsLeak tested Yes YesSlide background fluorescenceGlass: No endogenous fluorescencePermanox: Minimal fluorescencePolystyrene: Autofluorescence < 485 nmCoverslip compatibility Yes, following gasket removable YesFor adherent cell culture:RS treated glass slides - Proprietary washCC² - chemically modified growth surfaceGlass: No endogenous fluorescenceWriting surface “Sand blasted” frosted end Superfrost with printed endChambered Coverglass SystemFeature Lab-Tek Product Lab-Tek II ProductConfigurations 1, 2, 4, 8 well 1, 2, 4, 8 wellMedium chamber materialCoverglass materialsCoverProprietary cell culture grade polystyreneNon-removableBorosilicate glassNo. 1 thickness (0.13 - 0.17 mm)Clear polystyrene, no handle facilitatesstackingProprietary cell culture grade polystyrene -Non-removableBorosilicate glassNo. 1.5 thickness (0.16 - 0.19 mm)Clear polystyrene with handle foraseptic manipulationsSealant Non-toxic medical grade silicone Biocompatible acrylic adhesiveGrowth surface For adherent cell culture For adherent cell cultureBiological testing BHK-21, HEp-2 cells BHK-21, HEp-2 cellsLeak tested Yes YesSlide background fluorescence Glass·: No endogenous fluorescence Glass: No endogenous fluorescenceWriting surface None NonePackaging 8 tray, 16 pack, 96 case 8 tray, 16 pack, 96 caseSuperFrost is an Erie Scientific registered trademark.33

Always with updated information - www.nuncbrand.comFlask on SlideCell Culture177453• Ideal for:--Single cell autoradiography--Single cell immunofluorescence• Cell culture directly on amicroscopy slide• The flask on slide is ultrasonicallywelded and individually leak tested170920Literature:Tech Note Nos: 15, 20, 25, Vol. 4: 34, 35, 36, 37Slide Flask Procedureswww.nuncbrand.com/go/slideflaskSee Pages 195-196 for full reference list with titles and links34Flask on SlideSterileCat. No. 177453* 170920**Description Flaskette SlideFlaskSlide material Glass PolystyreneSuggested working vol., ml 2.5 - 5.0 2.5 - 5.0Culture area, cm²/well 10 9.0Units per pack/case 8/96 5/50* CE marked** NunclonΔ certifiedAccessoriesfor 170920Cat. No. 171862DescriptionCoverglassDimensions, mm 18 x 50Units per pack 100/1000

www.nuncbrand.com - Always with updated informationThe Art ofCell CultureCell CultureQuality in Research – Research in QualityFor additional information on Nunc cell culture products, literature and applications,visit our website: www.nuncbrand.com - always with updated information.35

Always with updated information - www.nuncbrand.comCryogenics36

www.nuncbrand.com - Always with updated informationCryogenics IntroductionIn 1962 the founder of Nunc telexedhome from a meeting in Geneva.He asked the production manager tomake a 2 ml tube for WHO, whichcould be used in the liquid phase ofnitrogen. This was the forerunner oftoday’s CryoTubes.L929 Cell Adsorption toCryoTubes (3 days incubation)NuncContentsCryoTubes Internal Thread................38CryoQuot............................................. 40CryoTubes Internal Thread,Barcoded................................................. 40CryogenicsWe have come a long way since then,most recently meeting the IATArequirements for the transport ofbiological samples. This means thatthe tubes can withstand evacuation to95 kPascal without leaking.All CryoTubes carry the CE markingrequired for containers of samples ofhuman origin.The certificate that accompanies eachbox indicates that the tubes are alsothoroughly tested. They are non-toxicas indicated by the USP biologicalreactivity class VI – 50°C test (7 daysimplant), which is performed on theraw materials used in the productionof all CryoTubes.The tubes are certified non-pyrogenic,meeting the international criteria.VariouscompetitorsNEWNEWCustom Barcoded CryoTubes...........41Barcode Scanners..................................41CryoTubes External Thread...............42CryoRacks for CryoTubes67 cm Aluminum................................... 44-20˚C Labtop Cooler.............................. 44MAX-100 CryoStore Boxes.................45Storage Boxes for CryoTubes........... 46Miniboxes for CryoTubes...................47CryoTube Rack.....................................47Accessories for Nunc CryoTubes..... 48CryoFlex.................................................49Cryobank vials and Bank-ItTube System.............................................50Example of low volume usage............51Cryobank Vials and Bank-ItAccessories...............................................53All tubes with internal and externalthreads are packed 50 per reclosablelaminated zippered bag . The strongerbags ensure sterility of the contents(As one of only very few cryo vialsuppliers, we can certify sterility at thelevel SAL 10-⁶). All bags are markedwith catalog and lot numbers.Nunc CryoTubes exhibit low bindingcharacteristics both in terms of cellularadhesion and protein binding whencompared with competitive tubes.Nunc Brand has comprehensivebarcoding capabilities. We can supplycoded tubes printed with Interleaved2 of 5 or labeled with Code 128. Bothcode types withstand all reagents andconditions normally used incryo-storage.37

Always with updated information - www.nuncbrand.comCryoTubes Internal ThreadCryogenics366524363452• Intended for cryogenic transportationand storage of biological material• CE marked for IVD• Conform to IATA requirements for thetransport of diagnostic specimens363401366656368632• Perform well in mechanical freezers,and in nitrogen (gas and liquid* phase)• One hand operation with starfoot tubesin CryoTube Rack (Cat. No. 376589)• Non-toxic, USP, Class VI• Non-pyrogenic• Non-mutagenic• Sterile (SAL 10¯⁶) according to ISO 11137• Re-closable zippered bags• Internal thread with a silicone gasketprovides the best possible seal379146• Catalog No. and Lot No. are printed onthe bags379189• For extreme storage conditions likeliquid phase of nitrogen CryoFlexmust be used*377224377267Starfoot tube fitsinto grooves of theCryoTube Rack(Cat. No: 376589)- Page 47 - whichallows one-handoperation.367997361247*WarningDo not use CryoTubes in the liquid phase of liquidnitrogen unless correctly sealed in Nunc CryoFlexTubing (Cat. No. 343958). Improper use may causeliquified nitrogen to be trapped inside the vial and leadto pressure build-up, resulting in possible explosion orbiohazard release. Use appropriate safety procedures asoutlined in the Nunc CryoPreservation Manual whenhandling and disposing of the CryoTubes.351934Literature:Tech Note No: 14363488336078336095336092336097336088336096336081See Pages 195-196 for full reference list with titles and linksAbout barcoding:www.nuncbrand.com/go/barcodingCryoPreservation Manual:www.nuncbrand.com/go/cryopresmanualInstruction guide enclosed in each caseAccessories:CryoTubes accessories Page 4838

www.nuncbrand.com - Always with updated informationNunc CryoTubesInternal thread.Polypropylene (PP) tubesand screw cap.Sterile with writing area.Cat. No. 363401 366524 363452 366656 368632Bottom shape Round Round Round Conical RoundSuggested working1.8 3.6 4.5 1.0 1.8volume, mlFree standing - - - + +Starfoot - - - - -Total length, mm 48 70 92 42 49Total diameter, mm 12.5 12.5 12.5 12.5 12.5Units per pack/carton/case 50/500/2000 50/400/1600 50/300/1200 50/500/2000 50/450/1800Cap fortubes withinternalthreadCryogenicsNunc CryoTubesInternal thread.Polypropylene (PP) tubesand screw cap.Sterile with writing area.Cat. No. 377224 377267 379189 379146Bottom shape Conical Round Round RoundSuggested working1.0 1.8 3.6 4.5volume, mlFree standing + + + +Starfoot + + + +Total length, mm 42 49 72 92Total diameter, mm 12.5 12.5 12.5 12.5Units per pack/carton/case 50/500/2000 50/450/1800 50/400/1600 50/300/1200Nunc CryoTubeswithout capsNon-SterileCat. No. 351934 367997 361247Bottom shape Round Round RoundSuggested working1.8 3.6 4.5volume, mlFree standing - - -Total length, mm 48 70 92Total diameter, mm 12.5 12.5 12.5Writing area + + +Units per pack/case 500/2500 250/1500 250/1500Nunc Replacement Screw CapsNon-SterileCat. No. 363436 336096 ‡ 336097 ‡ 336095 ‡Color Clear Red Blue YellowUnits per pack/case 500/2500 250/2500 250/2500 250/2500‡ Not available in AmericasNunc Replacement Screw CapsIrradiatedCat. No. 336078 336081 336088 336092 336094Color Clear Red Blue Yellow AssortedUnits per pack/case 500/2500 250/2500 250/2500 250/2500 250/250039

Always with updated information - www.nuncbrand.comCryoQuotCryogenics• CryoQuot allows a 250-300 µl aliquotto be stored in the cap• The aliquot is trapped by surfacetension• CryoQuot is filled by turning the tubeupside down and gently tapping thetube with a finger• Main sample and aliquot are storedunder the same conditions• The aliquot can be retrieved bytaking the cap off the frozen tube andmounting it on an empty tube. The capfrom the empty tube is mounted onthe main sample, which can be storedagain in the freezer without beingthawed• Barcoding available on requestNunc CryoQuotCat. No. 377272 377274Bottom shape Round RoundSuggested working volume, ml 1.8 4.5Free standing + +Starfoot + +Total length, mm 49 92Total diameter, mm 12.5 12.5Units per pack/carton/case 50/450/1800 50/300/1200CryoTubes Internal Thread, Barcoded40373514 373530See Pages 184 and 185 for further information on barcoded CryoTubesNunc CryoTubesInternal thread.Polypropylene (PP) tubesand screw cap. Barcoded.Cat. No. 373514 373530*Bottom shape Round RoundSuggested working vol., ml 1.8 1.8Free standing - +Sterile + -Starfoot - +Barcode Label InkjetBarcode type Code 128 Code Interleaved2 of 5Total length, mm 48 48Total diameter, mm 12.5 12.5Units per pack/carton/case 50/500/2000 50/450/1800* Caps included, unassembled

www.nuncbrand.com - Always with updated informationCustom Barcoded CryoTubesPad-print/Ink Jet is done by printingdirectly on the tube• Withstand temperature changes, likefreezing in liquid nitrogen and thesubse quent thawing• Code Interleaved 2 of 5• Available from 1.8 to 4.5 ml, internalthreadCryogenicsLabelPad-print/Ink JetLabel - the unique Nunc label withstandsmany chemicals e.g. toluene, acetoneand DMSO• Withstand temperature changes likefreezing and thawing• Resistant to autoclaving• Available as external and internalthread and from 1.0 - 4.5 ml• Available with Code 128 or Interleaved2 of 5 or Code 39 (see Page 184)Barcode Scanners• “Plug and Play” scanner for use withUSB slot• The scanners automatically switchbetween Code 128, Code 39,Interleaved 2 of 5 and severalother barcodes• Durable and low-cost• A contact scanner (it must be in contactwith the barcode to read)Nunc Barcode ScannersCat. No. 330040 ‡ 330050Description Scanner for keyboard slot Scanner for USB‡ Not available in Americas41

Always with updated information - www.nuncbrand.comCryoTubes External ThreadCryogenics337516347643375353375418347597347627Starfoot tube fitsinto grooves of theCryoTube Rack(Cat. No: 376589)- Page 47 - whichallows one-handoperationLiterature:Tech Note No: 14See Pages 195-196 for full reference list with titles and linksCryoPreservation Manual:www.nuncbrand.com/go/cryopresmanualInstruction guide enclosed in each caseAccessories:Cryoline accessories Page 48Screw capfor tubes withexternal threadNunc CryoTubesExternal thread.Polypropylene tubes.Polyethylene screw caps.Sterile.Cat. No. 375353 375418 337516 347597 347627 347643Bottom shape Conical Round Round Conical Round RoundSuggested working 1.0 1.8 4.5 1.0 1.8 4.5volume, mlStarfoot + + + + + +Total length, mm 41 48 91 41 48 91Total diameter, mm 12.5 12.5 12.5 12.5 12.5 12.5Writing area + + + - - -Units per pack/carton/case 50/500/2000 50/450/1800 50/300/1200 50/500/2000 50/450/1800 50/300/120042

www.nuncbrand.com - Always with updated informationCryoTubes External Thread• Designed for cryogenic storage of alltypes of cells or other specimens• Conform to the IATA requirements forthe transport of diagnostic specimens• Non-pyrogenic according to the LALtest• Non-toxic USP Class VI• Sterile (SAL 10¯⁶) according to ISO 11137• External thread recommended formechanical freezers*• Not recommended for use in the liquidphase of nitrogen• CE marked• Reclosable zippered bags• Catalog No. and Lot No. printed on thebagsCryogenics347155*WarningDo not use CryoTubes in the liquid phase of liquidnitrogen unless correctly sealed in Nunc CryoFlexTubing (Cat. No. 343958). Improper use may causeliquified nitrogen to be trapped inside the vial and leadto pressure build-up, resulting in possible explosion orbiohazard release. Use appropriate safety proceduresas outlined in the Nunc Cryopreservation Manualwhen handling and disposing of the CryoTubes.Nunc CryoTubesExternal thread, neck design.Polypropylene tubes.Polyethylene screw caps**.Sterile. CE MarkedCat. No. 375299 340711Bottom shape Round RoundSuggested working 1.0 1.8volume, mlFree standing - -Total length, mm 30 44Total diameter, mm 12.4 12.4Writing area + +Units per pack/carton/case 50/500/2000 50/500/2000** Closure design does not permit the use of CryoColorCode43

Always with updated information - www.nuncbrand.comCryoRacks for CryoTubes 67 cm AluminumCryogenics• Adjustable shelves to fit many box sizes• Accommodates 12 MAX-100 boxesfor 1.8 ml tubes ( Cat. No. 374187) or 8MegaMAX-100 boxes for 3.6 ml tubes(Cat. No. 341483) or a combina tion ofthese• Color coded tops for easy identification• Alignment rods secure boxes inposition• Easy-to-clean• Fits most chest freezers• Aluminum light weight materialNunc 67 cm Aluminum CryoRacks11 adjustable shelves /12 compartments. Dimensions, mm: H:670 - W:143.2 - D:140.4Cat. No. 367000 367005 367006 367007Top plate color Natural Blue Yellow RedUnits per pack/case 1 1 1 1-20˚C Labtop Cooler• Holds 20 CryoTubes (1.0 and 1.8 ml)• Inserts are included to accommodate0.2 to 0.5 ml microcentrifuge tubes• Protects enzymes, cells, reagentsand solutions by maintaining freezertemperatures while on the laboratorybench (T°

www.nuncbrand.com - Always with updated informationMAX-100 CryoStore Boxes• MAX-100 fits 1.0 and 1.8 ml tubes• MegaMAX-100 fits 3.6 ml tubes• MicroMAX-100 fits 1.5 mlmicrocentrifuge tubes• Use 10 x 10 dividers for internallythreaded tubes in gas phase ofnitrogen• Use 9 x 9 dividers for externallythreaded tubes or tubes fitted withCryoFlexCryogenicsNunc MAX-100 CryoStore BoxesCat. No. 374187 341483 330821Description MAX-100 w/10 x 10 Divider MegaMAX-100 w/10 x 10 Divider MicroMAX-100 w/8 x 8 DividerFor vial size, ml 1.0 - 1.8 3.6 1.5 - Microcentrifuge tubesMaterial Polycarbonate Polycarbonate PolycarbonateExternal dimensions, mm 132 x 132 x 52 132 x 132 x 78 132 x 132 x 52Accessories - dividers 342080/374357 340529/330856 335505Units per pack/case 4/24 4/24 4/24Accessories for Nunc MAX-100 CryoStore BoxesCat. No. 342080 374357* 340529 330856* 335505Description10 x 10 Dividerfor MAX-1009 x 9 Dividerfor MAX-10010 x 10 Dividerfor Mega MAX-1009 x 9 Divider fortubes in CryoFlex forMegaMAX-100Material PECCB PECCB PECCB PECCB PECCBUnits per pack/case 4/24 4/24 4/24 4/24 4/24PECCB = Polyethylene Coated Cardboard* Suitable for externally threaded tubes8 x 8 Dividerfor MicroMAX-10045

Always with updated information - www.nuncbrand.comStorage Boxes for CryoTubesCryogenics• Cardboard boxes in brown or white• Fit tubes from 1.0 - 4.5 ml4.5 ml4.5 ml4.5 ml3.6 ml3.6 ml1.8 ml1.0 ml 1.8 ml1.8 ml1.0 ml1.8 ml 1.8 ml1.0 ml1.0 mlStarfootNeckedStarfootRound bottomSelfstanding46Nunc Storage Boxes for Nunc CryoTubesCat. No. 378247 378220 369639 369647 340053 340061DescriptionStorage boxholding 25 tubesStorage boxholding 81 tubesStorage boxholding 81 tubesStorage boxholding 81 tubesStorage boxholding 100 tubesStorage boxholding 100 tubesColor Brown Brown White White White WhiteMaterial Cardboard Cardboard Cardboard Cardboard Cardboard CardboardFor tubes, ml 1.0 - 1.8 1.0 - 1.8 1.0 - 1.8 3.6 and 4.5* 1.0 - 1.8 3.6 and 4.5*Dimensions, incl. lid, mm 75 x 75 x 51 132 x 132 x 51 132 x 132 x 51 132 x 132 x 75 145 x 145 x 51 145 x 145 x 75Units per pack/case 24/24 6/6 48/48 30/30 42/42 30/30* 4.5 ml vials may be stored in the boxes; however, the lid will rest on the tubes

www.nuncbrand.com - Always with updated informationMiniboxes for CryoTubes• Keep special samples separate• Ideal for transport of CryoTubesCryogenicsNunc MiniBoxes for Nunc CryoTubesCat. No. 534479 534592DescriptionCan hold 5 CryoTubes,1.0 - 1.8 mlMaterial HIPS HIPSUnits per pack/case 350/350 200/200Symbols: HIPS= High Impact PolystyreneCan hold 10 CryoTubes,1.0 - 1.8 mlCryoTube Rack• Autoclavable• No. 376589 allows one handoperation when used withstarfoot tubesNunc CryoTube RackCat. No. 347155 376589*DescriptionNunc CryoTube Rackfor 70 tubesNunc CryoTube Rackfor 40 tubesMaterial Polycarbonate PolyphenyleneoxideExternal dimensions, mm 233 x 150 202 x 102Units per pack/case 30/30 1/15* One hand operation with starfoot tubesStarfoot tube fitsinto grooves of theCryoTube Rack(Cat. No: 376589)which allows onehandoperation47

Always with updated information - www.nuncbrand.comAccessories for Nunc CryoTubesCryogenics• Use Nunc Cryo Color Coders andCryoPens as part of a versatile andcomprehensive filing system• Cryo Color Coders fit all Cryo Tubes(except 375299 and 340711)• Canes can be marked with CryoStoreTabsLiterature:Tech Note No: 14See Pages 195-196 for full reference list with titles and linksCryoPreservation Manual:www.nuncbrand.com/go/cryopresmanualNunc Cane and PensCat. No. 378441 339993 ‡ 343850 343869 †Description Storage Cane CryoPens, 4 CryoPens CryoPenscolor in packColor - Black, red, BlackRedblue, greenMaterial Aluminum - - -Units per pack/case 50/50 4/100 1/10 1/10‡ Not available in Americas† Available only in AmericasNunc Tabs forStorage CaneCat. No. 378484 378492 378476 378468 378506Color Orange Yellow Green Blue RedUnits per pack/case 1/100 1/100 1/100 1/100 1/100Nunc Cryo Color CodersCat. No. 354968 355158 375868 354755 354879 355018Color Red Orange Brown White Blue GreenUnits per pack/case 500/2000 500/2000 500/2000 500/2000 500/2000 500/2000354968 355158 375868 354755 354879Nunc Cryo Color CodersCat. No. 375884 355077 375922 375906 375930Color Magenta Yellow Purple Grey AssortedUnits per pack/case 500/2000 500/2000 500/2000 500/2000 500/2000355018 375884 355077 375922 37590648

www.nuncbrand.com - Always with updated informationCryoFlex• Nunc CryoFlex provides extra safetywhen freezing hazardous samples orstoring in liquid nitrogenCryogenicsLiterature:CryoPreservation Manual:www.nuncbrand.com/go/cryopresmanualGuide to use Nunc CryoFlexCut to leave sufficientNunc CryoFlex at eachend of the tubePlace sample inCryoTube, tightenscrew cap, cool tubeon ice bath, insert theCryoTube into theNunc CryoFlexShrink the NuncCryoFlex by gentleheating with a heat gunfilled with a shrink tubeattachmentCrimp or squeeze theheated ends. Trim excesstubing and, if desired,melt the crimped endsRemove the NuncCryoFlex by cuttingaround the screw capUse Nunc CryoFlex as acane for liquid nitrogenNunc CryoFlexCat. No. 343958DescriptionNunc CryoFlexMaterialPolyethyleneExternal dimensions, mm 500 x 14Units per pack/case 15/30049

Always with updated information - www.nuncbrand.comCryobank vials and Bank-It Tube SystemCryogenicsAutomation friendly designThe new generation CryoTubes havebeen developed for automated use, andwill fit into freezer storage units fromdifferent manufacturers. The systemscan be used in the complete scale upfrom manual storage to a completelyautomated system.Manual system:• Vials can be labeled with a cryopenand stored in normal cryoboxeswith 13 x 13 inserts, therebyincreasing storage density andfreezer capacitySemi-Automated system:• Use with a 96 place decapper as astand-alone• Use with integrated 8 channeldecapper on liquid handlingplatform• Fully-automated systemsfrom companies such as TheAutomation Partnership, RTSThurnall, TekCel, etc.For updated information oncompatibility with storage systems andliquid handling platforms, contact us atcryo . nunc @ thermofisher.comVery little initial investment is neededto start up with this CryoTube system.It has the ability to take the user toa fully automated system, and it willallow the user to store DNA andproteins/cells in different volumes usingthe same automation.Dense storage format foroptimized freezer utilization• 96 individual CryoTubes in a 96well format frame (SBS footprint)• Working volume of 0.5 or 1.0 ml• Designed for use in the gas phaseof liquid nitrogen• The densest 1.0 ml cryo storagesystem on the marketUnique 14 x 14 array 2D CodeThe Nunc cryobank/Bank-it vials areavailable with laser-etched unique 2Dcodes. The 96 2D codes can be read inone pass by the Nunc scanner.2D codes are more error proof anddenser than normal linear codes.• The open Datamatrix ECC 200code allows for safe sampleidentification and reading of wholeracks in one process• 14 x 14 array 2D codesExample: NU XX 11111 CPrefixAlphanumeric5 digitsControlCertifications• CE marked for diagnostic use• Sterility assurance level 10-⁶ on thecryobank vials• DNAse/RNAse free• Non-pyrogenic (LAL-test) (onlyvalid for cryobank vials)• Non-toxic (USP class VI test)• Conform to IATA requirementsfor the transport of diagnosticspecimens, UN packinginstructions 602 and 650Nunc CryoTube with uniquelow binding surface• High sample recovery with lowconcentration samples• Cryobank vials for cells andproteins (black racks)• Bank-It for DNA (white racks)50

www.nuncbrand.com - Always with updated informationExample of low volume usageStorageRemoval of caps can be easilyautomated using a socket thatsnaps into the cap.Our partners offer 96 head decappersusing this technique.RetrievalWith the information stored in the database,it is possible for a technician to selecta number of vials for retrieval.The database program lists the racks, theirlocation in the freezers and the individualpositions of the vials. Fast retrieval avoidsprolonged thaw cycles that limit theviability of cells and can also ruin otherbiological samples.CryogenicsThe cryobank vials are arrayed ina microwell format that allows forthe use of multi-channel pippetorsor liquid handling robots. Thisfacilitates sterilework and minimizes handling time.The racks are selected in the databaseprogram. Each rack can contain more vialsthan are required to be picked.The Nunc tube selection tool is intended forpicking several vials out of a rack. While stillat room temperature, the bits are placed inthe positions listed in the database selectionlist. After verifying the bit position, therack is ready to be taken out of the cold.Caps are replaced using the singleor multiple head screwdrivers.Vials are locked into the rack,which ensures that they do not fallout during transport in the lab orstorage. The snap in the rack alsoretains the vial in the rack when thecap is mounted and the screwdriveris removed.The cryobank rack position is listed in thedatabase output and can be verifed byscanning the barcode on the cryobankrack.We guarantee verified uniquebarcodes on the racks and on thecryobank vials. 2D codes are laseretched into the bottom of the vial.The 2D codes are robust and offerthe ability to read multiple codesat the same time using the Nuncscanner.The scanner software reads thecodes and sends vial coordinateinformation to a databaseprogram.The Nunc tube selection tool has a cut-offcorner that ensures that the rack and tubeselection tool orientation is always alignedwhen the levers are pressed out of the rackand lifted up for easy retrieval. The rest ofthe rack is rapidly returned to the freezer toavoid significant thawing.The database program uses thelocation of the vials in the rack, thelocation of the rack in the freezer,and the associated sample data tokeep track of the position of everysample and combine it with thedata used for selecting samplesat retrieval.Codes can be verified either prior to or afterthaw. If the database is used properly, theuser avoids problems with condensation orfrosting during the reading process, as thetubes can thaw prior to checking the codes.Condensation and frost are problems thatcan be overcome by wiping the codes,having them covered during storage orusing dehumidified air.Nunc racks are available for bothdewars and freezers that optimizethe storage density. Racks aremade in aluminum and can becolor coded. Formats are bothfor microplate format racks andstandard cryobox formats.Vials should always be checked to makesure that actually the correct samples havebeen retrieved.51

Always with updated information - www.nuncbrand.comCryogenicsCryobank Vials and Bank-It Tube SystemNEWPlease contact your localdistibutor or sales rep.for a brochure or go towww.nuncbrand.com/go/innovativestoragewww.nuncbrand.comInnovative StorageTechnology for:Pharmagenomics • Cell Banking • BiobanksCryobank vials and Bank-ItDeveloped for automated handlingNunc Cryobank VialsCat. No. 374110 374120 374130 374027 374026 374025 374099 374100DescriptionNunc Cryobank VialsSterileCat. No. 374082* 374083* 374086 374087 374080 374097 374084* 374085* 374088 374089 374081 374094DescriptionNunc Cryobank Vials and Bank-It capsCat. No. 374017 374018 374019 374021DescriptionCryobankvials, rackedCryobankvials,rackedCryobankvials,rackedCryobankvials, rackedCryobankvials,rackedCryobankvials,rackedCryobank/Bank-Itcaps in racks, irradiatedCryobankvials, rackedCryobankvials,loosepackCryobankvials, rackedCryobankvials,racked,AlphacodeCryobank/Bank-Itcaps in racks - GreenCryobankvials, rackedCryobankvials,rackedCryobankvials,rackedCryobankvials, rackedCryobankvials,rackedCryobank/Bank-Itcaps in racks - RedCryobankvials, rackedCryobankvials,rackedUnits per pack/case 96/960 96/960 96/960 96/960Cryobankvials, SIloose packVolume 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 1.0 ml 1.0 ml 1.0 ml 1.0 ml 1.0 ml 1.0 ml2D coded + - + - - - + - + - - -Alphacode - - - - - + - - - - - +Cryobank/Bank-Itcaps in racks - BlueCryobankvials,racked,AlphacodeUnits per 96/192 96/192 96/960 96/960 96/960 96/960 96/192 96/192 96/960 96/960 96/960 96/960pack/case* Vial cases include black manual screwdriver Cat. No. 369643Nunc Bank-ItCat. No. 374066* 374067* 374074 374075 374070* 374090* 374078 374079Description Bank-It, racked Bank-It, racked Bank-It, racked Bank-It, racked Bank-It, racked Bank-It, racked Bank-It, racked Bank-It, rackedVolume 0.5 ml 0.5 ml 0.5 ml 0.5 ml 1.0 ml 1.0 ml 1.0 ml 1.0 ml2D coded + - + - + - + -Cryobankvials, rackedVolume 1.0 ml 1.0 ml 1.0 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml2D coded + + + + + + - -Cap color Green cap Red cap Blue cap Green cap Red cap Blue cap Red cap Blue capUnits perpack/case96/960 96/960 96/960 96/960 96/960 96/960 96/960 96/960Units per 96/192 96/192 96/960 96/960 96/192 96/192 96/960 96/960pack/case* Vial cases include black manual screwdriver Cat. No. 36964352

www.nuncbrand.com - Always with updated informationCryobank Vials and Bank-It AccessoriesHandheld electric screwdriverThe cap is retained by thesocket, allowing one handedmanipulation of the cap.MicroPlate rackNunc MicroPlate Storage Racksor storage of cryobank vials orBank-It racks. (See Page 174)CryogenicsManual screwdriverFor manual unscrewingand fastening of the caps.74 cm Cryobank rack74 cm rack is suitable for19 0.5 ml cryobank racksor 13 1.0 ml cryobank racks.Benchtop rack holderBenchtop rack holder isused for fixing the platewhile manipulating orfilling/emptying the vials.Storage boxWhite cardboard box for 169vials (13x13). Standard cryoboxsize. For use with loose vialsCat. Nos. 374081 and 374080.Tube selection toolThe Nunc tube selection tool is intendedfor quickly picking multiple vials out ofthe rack. This action avoids the thawingof the remaining vials. The Nunctube selection tool has a cut-off cornerthat ensures the rack and press out toolorientation are always aligned. The vialsselected are released by pressing thelevers and can be easily harvested.Scanner and softwareThe scanner software readsthe codes and sends positioninformation of the differentcodes to a database program.Accessories for Nunc Cryobank vials and Bank-ItCat. No. 369640 367014 374009 374011 374028 374001DescriptionUnits per pack/caseAccessories for Nunc Cryobank vials and Bank-ItScanners and softwareCat. No. 330041 330042 ‡ 330043 ‡DescriptionCardboard Cryo box,13x13 divider74 cm MicroPlateRackScanner and software,US plugTube selectiontoolBenchtop rackholderScanner and software,European plugBarcoded looserack1/48 1/1 1/1 1/1 10/10 50/50Units per pack/case 1/1 1/1 1/1Accessories for Nunc Cryobank vials and Bank-ItScrewdriversScanner and software,UK plug13 x 13 inserts forNalgene® StorageBox 550-0001Cat. No. 369643 369641 369644 ‡ 369658 ‡DescriptionSmall black manualscrewdriverElectric screwdriver,US plugElectric screwdriver,European plugElectric screwdriver,UK plugUnits per pack/case 1/1 1/1 1/1 1/1‡ Not available in Americas53

Always with updated information - www.nuncbrand.comImmuno Assay54

www.nuncbrand.com - Always with updated informationImmuno Assay IntroductionQuality - from start to finishNunc Brand uses virgin polymer resinsfor all immunology products and nevercompromise on purity or optical grade.Parameter settings on machines andinjection molding tools are based ondata gathered and analyzed in closecooperation between our research,quality control and production departments.To ensure optimal use of thisinformation all production lines arechecked at regular intervals.Quality control testsQuality starts with the choice of thebest resin, which can give the surfacethose characteristics needed to fulfillthe unique requirements for passiveadsorption of bio-molecules. The resinis the base upon which both opticalquality and uniformity of the surfacerely.The resin is thoroughly inspectedand approved before it is introducedinto the production facility. Duringproduction, samples are taken at regularintervals for visual inspection toensure that the requirements for opticalquality and the dimension standardsare met.After the molding steps are completed,the products undergo the necessarysurface modification to give thedesired characteristics. The surfaceproperties are tested in our laboratoryto ensure that the properties conformto the required standard. The productsare held in quarantine until the laboratoryresults confirm their compliance.Products which fail to meet the standardare scrapped.% OxygenSurface mattersImmuno assay technique is an extremelysensitive and specific methodwith a detection limit in the regionof 10-15 fmoles. It is essential to havethe appropriate surface and optimizedconditions in order to give a stable andreliable assay. We are experts in surfacetechnology, resulting in a broad rangeof products making assay optimizationeasy.Certified clean room facilityThe production environment is monitoredregularly for airborne particles.All production lines employ automationto ensure that from resin tofinished product there is only minimumcontact with dust and otherforeign bodies, which may affect assayperformance.All Nunc-Immuno modules areproduced in clean room facility(according to Federal Standard 209e).5432MultiSorp: 20% OMaxiSorp: 3% OPolySorp: 0% Olinear approximationMediSorp: 1% ONEWContentsImmuno Surfaces.........................................56About Immuno Assay Products ...............58Immuno LockWell Fluoronunc/Luminunc Modules...................................59Immuno 96 Microwell Plates..................60Immuno Modules.........................................62Immuno LockWell Modules ................64Immuno BreakApart Modules............66Color Coded Modules..................................67Fluoronunc/LuminuncModules/Plates.............................................68Immobilizer - AminoModules/Plates.............................................70CovaLink NH Modules..............................71Immobilizer - StreptavidinModules/Plates.............................................72Streptavidin Passively CoatedModules/Plates.............................................73Immobilizer - Nickel-Chelate Plates..... 74Immobilizer - Glutathione Plates..........75Immuno Washers......................................76MicroWell MiniTrays.................................77Immuno TSP...............................................78Immuno OmniTray......................................78Immuno Stick.............................................79Immuno Tubes MiniSorp......................80Immuno Tubes PolySorpand MaxiSorp.............................................81Immuno Assay100 10 20 30 40 50 60 70 80 90 100Receding contact angle degrees55

Always with updated information - www.nuncbrand.comImmuno SurfacesImmuno AssaySurfaces for ELISAThe choice of the optimal surface for thebinding or adsorption of the first layerin a sandwich ELISA is critical for thesuccess of the procedure. While highbinding is clearly important, the mostimportant parameter is the detectionlimit which depends on the signal tonoise ratio. Use the Nunc triangle (forpassive adsorption surfaces) or the hexagon(for active binding) as a guide whenselecting the surface for a new assay. Toachieve the best possible results alwaysoptimize the incubation conditions andconcentrations of the assay components.MaxiSorpNunc MaxiSorp is treated to give amixture of hydrophobic and hydrophilicareas for the adsorption of proteins.It is particularly optimized for theadsorption of IgG in a sandwich ELISA.MediSorpWhen high ELISA backgroundreadings are a problem, MediSorp maybe the solution. MediSorp’s lower totaladsorption capacity can, in many cases,reduce non-specific adsorption of serumproteins giving an improved signalto noise ratio.PolySorpThe PolySorp surface is ideal for theadsorption of hydrophobic molecules,including those that contain lipidmoieties.MultiSorpThis highly hydrophilic surfacehas a number of applications.Use MultiSorp for the adsorptionof hydrophilic biomolecules suchas highly substituted glycoproteins.Alternatively this surface could beused for the assay, in liquid phaseof very hydrophobic analytes.StreptavidinThe interaction between biotin andstreptavidin has been exploited in manyassay applications. Nunc provides twotypes of streptavidin surface.Streptavidin Passive AttachmentThe streptavidin molecule can beadsorbed directly to the polystyrenesurface. For many applications thisworks well, particularly for relativelysmall biotinylated molecules wheresteric hindrance due to closeness tothe polystyrene presents no problem.Immobilizer StreptavidinIn this case the streptavidin moleculesare covalently bound to the surfacevia spacer approx. 2 nm in length.This produces a number of advantages:reduced steric hindrance, a very stablesurface and controlled spacing of theavailable binding sites, which is idealfor large biotinylated biomolecules.LIPIDSGLYCOL IPIDSPolySorpLIPOPROTEINSMaxiSorpGLYC ANSMultiSorpGLYCOPR O TEINSMediSorpPROTEINSSchematic representation of the types of biomacromolecules, which can be bound to the available modified surfaces. E.g. if a lipid is to be bound thehydrophobic surface PolySorp is most suitable. Based on the physiochemical characteristics of the biomolecule to be immobilized, a surface can be chosen,which is appropriate for robust binding. As is indicated in the diagram, MaxiSorp has the widest breadth applications as it is capable of binding the greatestrange of molecules.56

www.nuncbrand.com - Always with updated informationImmobilizer AminoIn an ELISA, there are many advantagesof covalently binding the firstlayer to the surface. The Amino surfacecan be used to covalently bind proteins(including antibodies) or peptides.With a modified protocol DNA canalso be bound. Once the first layer isbound it is extremely stable allowingrigorous washing in the subsequentsteps.Immobilizer Nickel-Chelate/Immobilizer GlutathioneFor determination and characterizationof fusion proteins, Nunc Brandoffers two possibilities. Nickel-Chelatefor Histidine-tagged and Glutathionefor GST-tagged fusion proteins. Theseplates can be used for purified samples,as well as crude cell lysates.R - NH 2+GAH +EDCpH 9.6 pH 7.5CovaLinkR - SHAmino(Electrophile)PSBoth Immobilizer Nickel-Chelate andGlutathione have all the advantages ofthe Immobilizer family of products: noactivation steps, low detection limit,high signal to noise ratio and lowbackground without blocking.CovaLinkThe CovaLink surface chemistry oftenallows binding of molecules, whereother methods have failed. It can beused with bifunctional linkers to bindpeptides via a carboxylic acid group orDNA by the phosphorylated 5’ end ofthe molecule DNA. Alternatively, thewhole surface can be activated usingglutaraldehyde.StreptavidinNi-chelateBiotin - RGlutathioneGST - RDO’S & DON’TSin Immuno assaysDO:• Use the right surfacetreatment for your assay• Check your protocol for reagentconflicts e.g. buffer mismatches• Check your equipment for allpossible faults• Calibrate your pipettes,and check your precision• Store your reagents correctly• Time the assay steps precisely• Avoid high backgroundby blocking after the firstcoating. This leaves nouncoated areas whereunwanted binding can occur• Use a heating block or waterbathto transfer heat evenly. Thisminimizes edge effects• When using white plates,store in the dark prior toreading, as exposure to strongillumination can result inphosphorescence, which caninterfere with measurementsDON’T:• Let wells stand empty, theydry out quickly causingprotein damage• Use an automatically defrost -ing freezer for reagents• Develop plates in the light,edge effects can be caused bydifferences in illumination• Conduct assays in an incubator• Use detergent prior tothe blocking stepImmuno AssayR - COOH Poly-His - RThe diagram shows the available surface modifications for active binding of target molecules.In the case of CovaLink and Immobilizer Amino the target molecule is covalently bound to the PSvia a spacer group. The other surfaces firmly bind the target via ligand/receptor “like” interactionswhich depend on ionic/electrostatic and hydrogen bonds. The surface modifying compounds arecovalently bound to the PS.57

Always with updated information - www.nuncbrand.comImmuno AssayAbout Immuno Assay ProductsPassive Adsorption Surfaces - Immuno Assay ProductsSurface Format Base Polymer Hydrophilicity Binding Preference Key ApplicationsPolySorpMediSorpMaxiSorpMultiSorpMicroWell Plate,Module Plate, TubeMicroWell Plate,Module PlateMicroWell Plate,Module Plate, Tube,Omni TrayMicroWell Plate,Module PlatePolystyrene - Molecules containing significanthydrophobic regions, e.g. lipids,lipoproteins, large proteinsPolystyrene + Biomolecules with hydrophilic/hydrophobic properties, e.g. mediumto large proteins, immunoglobulins,albuminsPolystyrene ++ Biomolecules with hydrophilic/hydrophobic properties, e.g. smallto large proteins, immunoglobulins,albumins, LPS, phosphoproteins,glycoproteinsPolystyrene +++ Glycoproteins, polar lipids,phospholipids, cardiolipidAntigen ELISA,FIA, LIAAntibody sandwichELISA, Antigen ELISAAntibody sandwichELISA, FIA, LIAAntigen ELISA, FIA,LIAAntigen ELISALow Binding Surfaces - Immuno Assay ProductsSurface Format Base Polymer Hydrophilicity Binding Preference Key ApplicationsMiniSorp Tube Polyethylene n/a Low binding of molecules RIA, reagent storageand dilutionsCovalent Coupling Surfaces - Immuno Assay ProductsSurface Format Base Polymer Structure Binding Preference Key ApplicationsImmobilizerAminoMicroWell Plate,Module PlatePolystyreneAffinity Capture Surfaces - Immuno Assay ProductsSurface Format Base Polymer Structure Binding Preference Key ApplicationsImmobilizerStreptavidinMicroWell Plate,Module PlatePolystyreneBiotinylated biomoleculesImmobilizerNickel ChelateImmobilizerGlutathioneMicroWell Plate,Module PlateMicroWell Plate,Module PlateFIA - Fluorescence ImmunoassayLIA - Luminescence ImmunoassayNA - Nucleic AcidPolystyrenePolystyreneReactive electrophiletethered to the surfaceby 2nm ethylene glycolspacer armCovaLink Module Plate Polystyrene 2nd amine tethered by 2nm hydrocarbon spacerarmStreptavidin covalentlycoupled to polystyrenesurface via 2 nm ethyleneglycol spacer armNickel Chelate covalentlycoupled to polystyrenesurface via ethylene glycolspacer armGlutathione covalentlycoupled to polystyrenesurface via ethylene glycolspacer armCovalent coupling ofbiomolecules with freeNH2, and/or SH groups,e.g. proteins, peptides,aminated oligosSpecificity of covalent bondis dependent on couplingagent used: EDC-COOH, PO46x His fusion proteinsGlutathione transferasefusion proteinsAntigen ELISA,FIA, LIA, NAHybridization assaysAntigen ELISA, LIA,FIAImmunoassays,protein-proteinbinding assays,PCR ELISA, NAHybridization assaysImmunoassays,protein-protein andprotein-nucleic acidbinding assaysImmunoassays,protein-protein andprotein-nucleic acidbinding assaysImmobilizer is a trademark of Exiqon A/S, Vedbaek, Denmark. The product is produced under license from Exiqon A/S and covered by EP 08 20483 andforeign application and patents.58

www.nuncbrand.com - Always with updated informationImmuno LockWell Fluoronunc/Luminunc ModulesNEW• For fluorescence/luminescence-basedimmuno-assays and binding assays• Designed using the standard 96MicroWell LockWell format• Each well locks in the frame of the sameheight to prevent jamming in machines• Easy to handle: single well held as easilyas strips• Uniform washing and reading• Certified binding homogeneity for all 4surfacesImmuno AssayWhite• White opaque and reflective surface• Low cross talk and minimal backgroundBlack• Black opaque and light absorbingsurface• Minimum cross talk and back scatteredlightNEWImmuno LockWellFluoronunc/LuminuncModules, framed96 wells per frame.Polystyrene. Externaldimensions 128 x 86 mmCat. No. 463200* ‡ 446473* ‡ 463201* ‡ 446471* ‡Configuration C8 C8 C8 C8Surface PolySorp PolySorp MaxiSorp MaxiSorpTotal volume, µl/well 350 350 350 350Color White Black White BlackUnits per pack/case 10/60 10/60 10/60 10/60* With certificate‡ Not available in Americas59

Always with updated information - www.nuncbrand.comImmuno 96 Microwell PlatesImmuno Assay• Plates intended for quantitative andqualitative solid phase immuno assays(e.g. ELISA) and binding assays• Choice of Surface--PolySorp, with high affinity to moleculesof a hydrophobic nature--MediSorp, has a surface chemistrybetween PolySorp and MaxiSorp.Provides low background signal withsamples containing serum.--MaxiSorp, with high affinity tomolecules with mixed hydrophilic/hydrophobic domains.--MultiSorp, with high affinity tohydrophilic molecules• Choice of F, C and U bottoms• Raised rims on the wells• Certified reproducibility of binding• Alphanumeric orientation system• When centrifuging MicroWell Platesuse Spacer Plate (Cat. No. 259684) assupport• Fit standard equipment• Barcodes available on request (see Page184 in Custom Services section)Literature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10, 11, 12See Pages 195-196 for full reference list with titles and linksFFlat bottomCFlat bottomwith curvededgesURound bottomAccessories:Immuno Washers Page 76Spacer Plate Page 107NucleoLink Page 106Sealing Tapes Page 178Lids Page 176Contact your local distributoror sales rep. for a copy of theGuide to Solid Phase.Also available on:www.nuncbrand.com/go/solidphaseguideGuide toSolid Phase60

www.nuncbrand.com - Always with updated informationCertificateMaxiSorp SurfaceThis certificate is your guarantee that representative samples of thisproduct have been validated according to the following test.Control of homogeneity in adsorption of IgGCoefficient of variation (CV) within a plate format is less than 5%.OD results in the wells of the plate are within ± 10% from mean.Nunc A/S Quality System iscertified* to ISO:9001:2000 andISO 13485:2003 and designed tocomply with US current GoodManufacturing Practice for MedicalDevices and In Vitro Diagnosticproducts, classified as such in theUS and EU. Product Quality isconsistently monitored and complieswith the high quality standards thatwe have specified for Nunc products.Our quality is documented in lotspecific certificates.Immuno AssayFor information on tests used please refer to www.nuncbrand.comThe Management System of Nunc A/S, Roskilde, Denmark,has been approved by Lloyd’s Register Quality AssuranceLimited to the Quality Management System StandardISO 9001:2000, ISO 13485:2003 and EnvironmentalManagement System IS0 14001:1996* Valid for products manufacturedin Denmark.Nunc-Immuno PlatesPolystyrene, without lids.96 wells per plate.Ext. dimensions 128 x 86 mmCat. No. 475094 456529 446140 475434 467320* 467340Configuration F96 F96 C96 U96 F96 F96Surface PolySorp PolySorp PolySorp PolySorp MediSorp MultiSorpDesign Pinchbar High Flange Pinchbar High Flange High Flange High FlangeTotal volume, µl/well 400 400 350 300 400 400Suggested working 350 350 250 250 350 350volume, µl/wellTotal surface area, cm²/well 2.7 2.7 2.5 2.0 2.7 2.7Barcode - - - - - -Units per pack/case 5/60 10/180 5/60 5/60 5/60 5/60* With certificateDesign with pinchbarDesign with high flangeNunc-Immuno PlatesPolystyrene, without lids.96 wells per plate.Ext. dimensions 128 x 86 mmCat. No. 456537* 460984* 439454* 442404 430341 446612* 449824Configuration F96 F96 F96 F96 C96 C96 U96Surface MaxiSorp MaxiSorp MaxiSorp MaxiSorp MaxiSorp MaxiSorp MaxiSorpDesign High Flange High Flange Pinchbar Pinchbar Pinchbar Pinchbar High FlangeTotal volume, µl/well 400 400 400 400 350 350 300Suggested working 350 350 350 350 250 250 250volume, µl/wellTotal surface area, cm²/well 2.7 2.7 2.7 2.7 2.5 2.5 2.0Barcode - + - - - - -Units per pack/case 10/180 10/180 5/60 5/60 5/60 5/60 5/60* With certificate61

Always with updated information - www.nuncbrand.comImmuno ModulesImmuno Assay• Developed for ELISA techniques• Choice of MaxiSorp, PolySorp,MediSorp and MultiSorp surfaces• Alphanumeric orientation systems• Fit standard equipment• High optical quality• With Nunc StarWell Modules incubationtime can be reduced by morethan 50% or the sensitivity similarlyincreased• Frames and modules availableseparately• Modules remain in frame wheninverted• Sealing tape and strip caps are available• Certified reproducibility of binding• Free-standing modules• Uniform reading and washing• Color coded modules are available onrequest (see Page 67)Literature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10, 11, 12Tech Note No: 10See Pages 195-196 for full reference list with titles and linksAccessories:Sealing Tapes Page 178Strip Caps Page 46Immuno Washers Page 76Nunc-Immuno Modules, LoosePolystyreneCat. No. 469922 469329 ‡ 469957 469914* 469264* 469949*Configuration F16 U16 F8 F16 U16 F8Surface PolySorp PolySorp PolySorp MaxiSorp MaxiSorp MaxiSorpTotal surface area, cm²/well 2.7 2.0 2.7 2.7 2.0 2.7Total volume, µl/well 400 300 400 400 300 400Suggested working volume, 350 250 350 350 250 350µl/wellUnits per pack/case 80/320 80/320 160/640 80/320 80/320 160/640* With certificate‡ Not available in AmericasNunc FrameCat. No. 460348MaterialAcrylonitrile-Butadiene-Styrene (ABS)Units per pack/case 5/6062

www.nuncbrand.com - Always with updated informationImmuno Modules, framed96 wells per frame. PolySorp surface.Polystyrene. External dim.: 128 x 86 mmCat. No. 467679 ‡ 466966 473717 469078 444865 475086 441254Configuration F16 U16 C12 F8 C8 U8 C8 StarWellSurface PolySorp PolySorp PolySorp PolySorp PolySorp PolySorp PolySorpTotal surface area, cm²/well 2.7 2.0 2.5 2.7 2.5 2.0 3.0Total volume, µl/well 400 300 350 400 350 300 380Suggested working volume, 350 250 250 350 250 250 250µl/wellUnits per pack/case 10/60 10/60 10/60 10/60 10/60 10/60 10/60‡ Not available in AmericasImmuno AssayImmuno Modules, framed96 wells per frame. MaxiSorp surface.Polystyrene. External dim.: 128 x 86 mmCat. No. 467466* 464394* ‡ 473709* 468667* 434797* 445101* 475078* 441653*Configuration F16 U16 C12 F8 F8 C8 U8 C8 StarWellSurface MaxiSorp MaxiSorp MaxiSorp MaxiSorp MaxiSorp MaxiSorp MaxiSorp MaxiSorpTotal surface area, cm²/well 2.7 2.0 2.5 2.7 2.7 2.5 2.0 3.0Total volume, µl/well 400 300 350 400 400 350 300 380Suggested working volume, 350 250 250 350 350 250 250 250µl/wellUnits per pack/case 10/60 10/60 10/60 10/60 20/120 10/60 10/60 10/60* With certificate.‡ Not available in AmericasImmuno Modules, framed96 wells per frame. Medi- and MultiSorp surfaces.Polystyrene. External dimensions: 128 x 86 mmCat. No. 467120* 467140Configuration F8 F8Surface MediSorp MultiSorpTotal surface area, cm²/well 2.7 2.7Total volume, µl/well 400 400Suggested working volume, 350 350µl/wellUnits per pack/case 10/60 10/60* With certificate.FFlat bottomCFlat bottomwith curvededgesURound bottomAccessoriesNunc-Immuno ModulesCat. No. 236366 236370 † 236269 ‡ 430805 430082Description Sealing tape Sealing tape Sealing tape 8 Well stripcap8 Well stripcapMaterial Polyester Polyester Polyester Polyethylene PolyethyleneSterile + - - + -Units per pack/case 200/800 100/100 200/800 12/120 60/180† Available only in Americas‡ Not available in AmericasC StarwellFlat bottomwith curvededges and8 ribsC8 StarwellShowing theorientation ofthe 8 ribs63

Always with updated information - www.nuncbrand.comImmuno LockWell ModulesImmuno Assay• Facilitates flexible diagnostic assaysthat require any number of wells;96 to 1• Choice of MaxiSorp, PolySorp,MediSorp and MultiSorp surfaces• Standard 96 MicroWell format• Each well locks in the frame at the sameheight to prevent jamming in machines• Easy to handle: single well held as easilyas strips• Strips or single wells are easy to take inand out of the frame without use of anyspecial tools• 8 well strips available with U or Cbottoms as well as C-StarWells• Marked for easy identification of eachwell• Uniform washing and reading• Certified binding homogeneity• Color coded modules are available onrequest (see Page 67)Literature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10 , 11, 12Tech Note No. 10See Pages 195-196 for full reference list with titles and linksLetters and notches on wells allow easy recognition of a single wellAccessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 176Nunc LockWell FrameExternal dimensions 128 x 86 mmCat. No. 465404DescriptionFrameMaterialAcrylonitrile-Butadiene-Styrene (ABS)Units per pack/case 10/60The LockWell frame has a unique spring lock. It locks each individualwell in the frame through an undercut in the well. The spring lock doesnot touch the strip or well when inserted in the frameUS Patent No. 5.514.343Eur. Patent Appl. No. 2113/0688602.64

www.nuncbrand.com - Always with updated informationEasy handlingImmuno AssayRelease a stripNunc-Immuno LockWellModules, framed96 wells per frame. Polystyrene.External dimensions 128 x 86 mmCat. No. 446442 446477 ‡ 448496 ‡Configuration C8 U8 C8 StarWellSurface PolySorp PolySorp PolySorpTotal surface area, cm²/well 2.5 2.0 3.1Total volume, µl/well 350 320 330Suggested working 250 250 200volume, µl/wellUnits per pack/case 10/60 10/60 10/60‡ Not available in AmericasLift a stripNunc-Immuno LockWellModules, framed96 wells per frame. Polystyrene.External dimensions 128 x 86 mmCat. No. 446469* 446639* ‡ 448526* ‡Configuration C8 U8 C8 StarWellSurface MaxiSorp MaxiSorp MaxiSorpTotal surface area, cm²/well 2.5 2.0 3.1Total volume, µl/well 350 320 330Suggested working 250 250 200volume, µl/wellUnits per pack/case 10/60 10/60 10/60* With certificate‡ Not available in AmericasNunc-Immuno LockWellModules, framed96 wells per frame. Polystyrene.External dimensions 128 x 86 mmCat. No. 446470* 446490Configuration C8 C8Surface MediSorp MultiSorpTotal surface area, cm²/well 2.5 2.5Total volume, µl/well 350 350Suggested working 250 250volume, µl/wellUnits per pack/case 10/60 10/60* With certificateHow to detach a wellInsert a stripIdentifiable individual wellsTapping65

Always with updated information - www.nuncbrand.comImmuno BreakApart ModulesImmuno Assay• For solid phase immuno assays, e.g.ELISA• MaxiSorp or PolySorp surfacesavailable• Consists of a frame with 12 firmlyseated carriers, each holding a breakablestrip of 8 wells.• Push-out tools are supplied in everycase of 60 modules• Free standing modules• Alphanumeric orientation system• Fit standard equipment• High optical quality• Uniform reading and washing• Certified binding homogeneity• Color coded modules are available onrequest (see Page 67)Literature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10, 11, 12See Pages 195-196 for full reference list with titles and linksAccessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 176Nunc-Immuno BreakApartframed96 well per frame. Polystyrene.Ext. dimensions 128 x 86 mmCat. No. 473539 473768*Well shape C8 C8Surface PolySorp MaxiSorpTotal surface area, cm² 2.5 2.5Total volume, µl 350 350Suggested working volume, µl 250 250Units per pack/case 10/60 10/60* With certificateAccessoriesNunc-Immuno BreakApart.PolyethyleneCat. No. 430414 431615Description Push-out tool C8 BreakApart FrameUnits per pack/case 240/240 5/6066

www.nuncbrand.com - Always with updated informationColor Coded Modules• Available as PolySorp or MaxiSorp indifferent configurations and 15 colors• Standardized to facilitate easyidentification of your assays• High reliability when working withindividual wells• Post-molding process where a layer ofpigment is applied to the plastic rim byheat stamping• Only one color per plate is available• Pre-batch approval is possible• Minimum order of 2 cases (5 cases in US)Immuno AssayLiterature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10, 11, 12See Pages 195-196 for full reference list with titles and linksAccessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 176Nunc Color Coded ModulesThe colors are available in following formatsBA = BreakApart, LW = LockWellColorC8 BAPolySorpC8 LWPolySorpU8PolySorpC8MaxiSorpC8 BAMaxiSorpC8 LWMaxiSorpU8MaxiSorpF8MaxiSorpRef. Prod. 473539 446442 475086 445101 473768 446469 475078 468667Green 465005 475443 478191 445120 459048 475000 478178 462456Yellow 464912 475444 478192 445113 463630 473237 478176 459990Blue 460799 475001 478193 445110 460798 474691 478179 462455Red 464904 475445 478194 445117 460801 475426 475540 463975Dark Blue 465006 475446 478195 445121 463649 476422 478180 465560White 465013 475447 478196 445114 463657 475442 478189 462465Black 465007 475454 478202 445118 465919 475957 478181 462475Orange 464920 475448 478197 445115 465020 475967 478177 465552Burgundy 465008 475449 478198 445119 459049 477089 478183 462485Grey 465009 475451 478199 445112 463622 478174 478184 462495Brown 465010 475452 478200 445111 465188 478123 478185 462445Purple 465011 475453 478201 445116 465021 475965 478175 468993Turquoise 465012 475455 478205 445122 465189 476423 478186 462457Maroon 465014 475456 478206 445123 465190 476424 478187 462458Pink 465015 475457 478207 445124 463469 476425 478188 462459Minimum order of two cases; all products are packed 10/60.Other modules can be color coded - Please ask for a quote at your local Nunc Brand distributor or at info.nunc@thermofisher.com67

Always with updated information - www.nuncbrand.comFluoronunc/Luminunc Modules/PlatesImmuno Assay• For fluorescence/luminescence-basedimmuno-assays and binding assays• MaxiSorp surface with high affinityto molecules with mixed hydrophilic/hydrophobic domains• PolySorp, less hydrophilic thanMaxiSorp and with affinity tomolecules of a more hydrophobicnature• Certified binding homogeneity• Fits standard equipment• Compatible with lids for MicroWellplates and sealing tape and breathablemembraneFAQWhy does Nunc Brand produce black or white wells?In order to enable the most sensitive reading of assay results.This means selecting and testing the best possible resins and colorantsfor specific applications.White• Gives maximum reflection• Minimum autofluorescence andautoluminescenceBlack• Minimizes background in fluorescencereading• Minimizes back scattered light• Minimizes cross-talkClear• Time resolved fluorescenceLiterature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10, 11, 12Tech Note Nos: 6, 12See Pages 195-196 for full reference list with titles and linksAccessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 17668

www.nuncbrand.com - Always with updated informationNunc Modules forFluorescence, framed96 wells per plate. Polystyrene.External dimensions 128 x 86 mmCat. No. 475523 437877 ‡ 437702 475515* 437915* 437591*Configuration F16 C12 C8 F16 C12 C8Surface PolySorp PolySorp PolySorp MaxiSorp MaxiSorp MaxiSorpTotal volume, µl/well 400 350 350 400 350 350Suggested working 350 250 250 350 250 250volume, µl/wellTotal surface area, cm²/well 2.7 2.5 2.5 2.7 2.5 2.5Color Black Clear** White Black Clear** WhiteUnits per pack/case 10/60 10/60 10/60 10/60 10/60 10/60* With certificate** For time resolved fluorescence‡ Not available in AmericasImmuno AssayNunc 96 Well Platesfor Fluorescence96 wells per plate. Polystyrene.External dimensions 128 x 86 mmCat. No. 437112 437842 437869 ‡ 437111* 437796* 437958*Configuration F96 C96 C96 F96 C96 C96Surface PolySorp PolySorp PolySorp MaxiSorp MaxiSorp MaxiSorpTotal volume, µl/well 400 350 350 400 350 350Suggested working 350 250 250 350 250 250volume, µl/wellTotal surface area, cm²/well 2.7 2.5 2.5 2.7 2.5 2.5Color Black White Clear** Black White Clear**Units per pack/case 10/80 5/60 5/60 10/80 5/60 5/60* With certificate.** For time resolved fluorescence‡ Not available in AmericasNunc 96 Well Platesfor Fluorescenceand Luminescence96 wells per plate. Polystyrene.External dimensions 128 x 86 mmCat. No. 436111 436110*Configuration F96 F96Surface PolySorp MaxiSorpTotal volume, µl/well 400 400Suggested working 350 350volume, µl/wellTotal surface area, cm²/well 2.7 2.7Color White WhiteUnits per pack/case 10/80 10/80* With certificate69

Always with updated information - www.nuncbrand.comImmobilizer - Amino Modules/PlatesImmuno Assay• Rapid covalent coupling of peptides,nucleotides, proteins, enzymes andantibodies• Ready to use - no activation steps orblocking steps• Low reagent consumption• High signal to noise ratio• Long term stability at roomtemperature (up to 2 years)• Surface optimized to minimize sterichindrance and optimize bindinginteractions• 384 well plates available (see Page 162)• Form covalent bond with nucleophilicgroups, e.g. amines• Immobilize molecules that bind poorlyto passive surfaces(O)n OH 2 NCOOHLiterature:Bulletin No: 1Tech Note Nos.: 43, 56See Pages 195-196 for full reference list with titles and links(O)n OCOOHAccessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 176Covalent coupling of a peptide to the ImmobilizerAmino Plate. During a short incubation step, the peptidewill bind to the electrophilic groupNEWNow also for bindingaminated nucleotidesSee Tech Note 56Immobilizer is a trademark of Exiqon A/S, Vedbaek,Denmark. The product is produced under license fromExiqon A/S and covered by EP 08 20483 and foreignapplication and patents.Nunc Immobilizer,Amino Modules/PlatesCat. No. 436006 436007 436008 436013 436023Configuration F96 F96 F96 F8 C8 LockWellColor Clear White Black Clear ClearTotal volume, µl/well 400 400 400 400 350Suggested coupling 100 100 100 100 100volume, µlUnits per pack/case 5/30 5/30 5/30 5/30 5/3070

www.nuncbrand.com - Always with updated informationCovaLink NH Modules• Developed to bind compoundscovalently to the surfaces• For use with molecules, which are noteasily adsorbed to a passive surface• Agressive washing possible using highionic strength solutions• Low non-specific binding• Reaction site easily accessible• Allows site-specific binding via acarboxylic group of a peptide or thephosphate group in the 5’ end ofnucleic acid• Alphanumeric orientation system• Fit all standard equipment• Free-standing modules• White modules for fluorescence andluminescence applicationsImmuno AssaySchematic chemical and physicalconfiguration of the CovaLink NH surface.The NH groups are spaced from thepolystyrene surface by approx. 2 nm long,chemically defined spacer arms, and arecovalently anchored to the surface.ASchematic illustration of the advantageof orientated covalent immobilizationcontrary to physical adsorption of molecules.A: Covalent coupling may be directed to agroup away from the molecule’s active site tosecure an orientation of the molecule, whichmakes its active site accessible for the targetmolecules (0) added in the liquid phase.B: Physical adsorption involves the risk ofhiding of the active site against the surface(left), or destruction of the active site dueto distortion of the molecule (right) byadsorption.BLiterature:Bulletin Nos: 1, 4, 7, 10, 11Tech Note Nos: 9, 11, 17See Pages 195-196 for full reference list with titles and linksCovaLink Manualwww.nuncbrand.com/go/covalinkmanualAccessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 176Contact your local distributoror sales rep. for a copy of theCovalent Binding Brochure.Nunc CovaLink NHModules, framed96 wells per frame. Polystyrene.External dimensions 128 x 86 mmCat. No. 478042 244105 ‡ 453690 ‡Configuration F8 C8 BreakApart C8Plate color Clear Clear WhiteTotal volume, µl/well 400 330 350Suggested working 100 100 100volume, µl/wellTotal surface area, cm²/well 2.7 2.5 2.5Units per pack/case 5/30 5/30 5/30NH = secondary amino group‡ Not available in AmericasAlso available on:www.nuncbrand.com/go/covalentbindingYour Partner inImmunology71

Always with updated information - www.nuncbrand.comImmobilizer - Streptavidin Modules/PlatesImmuno Assay• Rapid coupling of biotinylatedbio-molecules• Streptavidin is covalently bound to thesurface• Ready to use - no blocking steps• Excellent binding capacity( e.g. F96 clear 20 pmol/well*)• Minimal leaching of coupledbiomolecules from the surface• Binding site easily accessible• Low non-specific binding• High signal to noise ratio• Stable at room temperature• Low reagent consumption• White and black plates forluminescence or fluorescenceapplications• 384 well plates available (see Page 162)* Depending on size or sterical properties of a givenbiomolecule the actual moler binding capacity mightbe differentLiterature:Bulletin Nos: 1, 4Tech Note Nos: 41, 42See Pages 195-196 for full reference list with titles and linksCoupling of a biotinylated protein to the covalently boundstreptadivin. After a pre-wash, simply add the biotinylated targetmolecule in an appropriate buffer. In a short incubation step, thebiotinylated molecule will bind to the streptavidin molecule.Accessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 176Immobilizer is a trademark of Exiqon A/S, Vedbaek,Denmark. The product is produced under license fromExiqon A/S and covered by EP 08 20483 and foreignapplication and patents.Nunc Immobilizer,StreptavidinPlates and ModulesCat.No. 436014 436015 436016 436020 436022Configuration F96 F96 F96 F8 C8 LockWellColor Clear White Black Clear ClearTotal volume, µl 400 400 400 400 350Suggested coupling 100 100 100 100 100volume ul/wellUnits per pack/case 1/15 1/15 1/15 1/15 1/1572

www.nuncbrand.com - Always with updated informationStreptavidin Passively Coated Modules/Plates• Ideal for binding of biotinylatedbiomolecules, such as peptides, antibodies,oligonucleotides or haptens• Streptavidin is passively coated on anarea of 154 mm² (area covered by avolume of 200 µl)• Binding capacity for biotin of at least 13pmol/well*• Stable at room temperature• General assay protocol available onrequest• Available in C96 plates or C8 well stripsImmuno Assay* Depending on size or sterical properties of a givenbiomolecule the actual molar binding capacity mightbe different.Application Description GraphicELISABiotinylated antibodies areimmobilized on a surface.Capture of antigen followed bydetection with labeled secondantibodyPCR-ELISA Amplify using DIG-dUTP and abioti nylated primer. Capture ona streptavidin plate. Detectionby enzyme labeled anti-DIG.DNAhybridizationassaysAnalysis of DNAbinding proteinsCapture of DNA in sample bybioti nylated probe. Collectionon a streptavidin coated plate.Detection by labeled detectionprobe.Immobilize biotinylated DNAon a strepta vidin coated plate.Incubate with DNA-bindingprotein. Detect with antibody.Literature:Bulletin Nos: 1, 4See Pages 195-196 for full reference list with titles and linksAccessories:8 well strips Page 248 well strips caps Page 63Immuno Washers Page 76Sealing Tapes Page 178Tape 12 and 48 Page 179Lids Page 176The examples given above do not constitute a full list and eachapplication can be performed in several waysStreptavidinBiotinAntigenDIG-labelDNADNA binding proteinEnzyme labeledanti-DIG-antibodyNunc Streptavidin PassivelyCoated Modules/Plates96 wells per frame. Polystyrene.External dimensions 128 x 86 mmCat. No. 236001 ‡ 236004 ‡Configuration C96 C8Color Clear ClearTotal volume, µl/well 350 350Coated well volume, µl 200 200Units per pack/case 1/15 1/15‡ Not available in Americas73

Always with updated information - www.nuncbrand.comImmobilizer - Nickel-Chelate PlatesImmuno AssayNi-ChelateHisProtein/Petide• Rapid coupling of 6x Histidine-taggedfusion proteins• Covalently coupled Nickel-Chelate onthe surface• No blocking steps• For detection and quantification of6x Histidine-tagged fusion proteins• Low detection limit• Low background• High signal to noise ratio• Stable at room temperature• Low reagent consumption• Binding site easily accessible• White and black plates forluminescence and fluorescence assays• 384 well plates available (see Page 162)Literature:Bulletin Nos: 1, 4Tech Note Nos: 46, 47See Pages 195-196 for full reference list with titles and linksAccessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 176Coupling of a 6x Histidine-tagged protein/peptide to theNunc Immobilizer Nickel-Chelate plate.His = HistidineNunc Immobilizer,Nickel-Chelate Plates96 wells. External dim. 128 x 86 mmCat. No. 436024 436026 436027Configuration F96 F96 F96Color Clear White BlackTotal volume, µl/well 400 400 400Suggested coupling 100 100 100volume, µl/wellUnits per pack/case 1/15 1/15 1/15Immobilizer is a trademark of Exiqon A/S, Vedbaek,Denmark. The product is produced under license fromExiqon A/S and covered by EP 08 20483 and foreignapplication and patents.74

www.nuncbrand.com - Always with updated informationImmobilizer - Glutathione PlatesGSHGSTProtein/Petide• Coupling of GST-tagged fusion proteins• Covalently coupled glutathione on thesurface• For detection and quantification ofGST-tagged fusion proteins• Ready to use - no activation steps orblocking steps• Low detection limit• Low background noise• High signal to noise ratio• Stable at room temperature (1 year)• Low reagent consumption• Reaction site easily accessible• White and black plates for luminescenceand fluorescence applications• 384 well plates available (see Page 162)Literature:Bulletin Nos: 1, 4Tech Note Nos: 2, 44, 45See Pages 195-196 for full reference list with titles and linksAccessories:Immuno Washers Page 76Sealing Tapes Page 178Lids Page 176Immuno AssayProtein/PetideGSHGSTCoupling of a GST-tagged protein/peptide to theNunc Immobilizer, Glutathione plates.GSH = GlutathioneGST= Glutathione-S-transferaseNunc Immobilizer,Glutathione Plates96 wells.External dimensions 128 x 86 mmCat.No. 436032 436033 436034Configuration F96 F96 F96Color Clear White BlackTotal volume, µl/well 400 400 400Suggested coupling 100 100 100volume, µl/welUnits per pack/case 1/15 1/15 1/15Immobilizer is a trademark of Exiqon A/S, Vedbaek,Denmark. The product is produced under license fromExiqon A/S and covered by EP 08 20483 and foreignapplication and patents.75

Always with updated information - www.nuncbrand.comImmuno WashersImmuno Assay• Easy-to-use• Allow for a flexible washing procedure• Uniform and efficient washing• Very robust and reliable• Rack included (not autoclavable)• Adjustable to all well shapes• Autoclavable at 121°C• No electrical connectionsNunc-Immuno WashersCat. No. 470174 470175Description Nunc-Immuno Wash 8 Nunc-Immuno Wash 12Units per case 1 1The Nunc-Immuno Wash is available in 8 and 12 channel versions to fit the 96 wellImmuno plate or module format. Adjustable to all well shapes. The 8 channel version isnot suitable for BreakApart modules.76Accessories for Nunc-Immuno WashersCat. No. 554569 ‡ 455493DescriptionTubing Kit for Nunc-Immuno Spare Part KitWashersContains5 m silicone tubing (10 mmdiameter, 6 mm bore),5 different O-rings1 spring1 m silicone tubing (5 mmdiameter, 3 mm bore),3 clamps, 1 Y connector.Units per case 1 1‡ Not available in Americas

www.nuncbrand.com - Always with updated informationMicroWell MiniTrays• Used in serotyping, microcytotoxicityand cell cloning studies• Optical clarity allows use with invertedmicroscopes• Occupy minimal freezer and incubatorspace• Lids have keyed tabs for properorientation• Large raised alpha-numeric coordinates• Unique surface treatment to enhancesample and reagent mixingImmuno AssayNunc MicroWell MiniTraysfor Serological ApplicationsPolystyrene. With lidCat. No. 439225 438733 448698Number of wells 60 72 72Well dimensions, mm Top: 3.71Bottom: 1.27Depth: 1.91Top: 3.71Bottom: 1.27Depth: 1.91Sterile - - +Top: 3.71Bottom: 1.27Depth: 1.91External dimensions, mm 84 x 59 w/lid 84 x 59 w/lid 84 x 59 w/lidProfile Low Low LowTotal surface area, cm² 0.2 0.2 0.2Total volume, µl 10 10 10Units per pack/case 10/100 10/100 10/100Add reagentand sampleReagent and samplewill merge due tothe special surfacetreatment of the wellsNunc MiniTraysPolystyreneCat. No. 452256 470378 472400Description MiniTray with lid MiniTray with lid MiniTray withS-PlateNumber of wells 60 72 60Well dimensions, mm Top: 3.82Bottom: 1.34Depth: 1.92External dimensions, mm81 x 56 without lid84 x 59 with lidTop: 3.82Bottom: 1.35Depth: 1.9281 x 56 without lid84 x 59 with lidProfile Low Low HighTotal surface area, cm² 0.2 0.2 0.2Total volume, µl 10 10 10Top: 3.72Bottom: 1.30Depth: 2.1081 x 56 without lidUnits per pack/case 120/480 120/480 100/400 + 16 lidsNunc Glass Coverslips for MiniTraysCat. No. 448701External dimensions, mm 50 x 75Units per pack/case 100/500Nunc Lid for MiniTray S-platesPolystyreneCat. No. 472419External dimensions, mm 83 x 59Units per pack/case 100/40077

Always with updated information - www.nuncbrand.comImmuno TSPImmuno Assay• Facilitates the testing of analytes simultaneously,using the ELISA technique• Ideal for screening of hybridomacultures for antibody production• Available in MaxiSorp or PolySorpsurfaces• Uniform adsorption propertiesLiterature:Bulletin Nos: 6, 7, 8, 9, 10, 11, 12Tech Note No: 24See Pages 195-196 for full reference list with titles and linksNunc-Immuno TSPPolystyreneCat. No. 473245 476635 ‡Surface PolySorp MaxiSorpTotal surface area, mm² 95 95Units per pack/case 10/210 10/210‡ Not available in AmericasImmuno OmniTrayNunc-Immuno TSPFor Hybridoma screeningmounted in the trayPolystyrene. SterileCat. No. 445497Total surface area, mm² 95Units per pack/case 1/50Washing Tray forNunc-Immuno TSPPolystyreneCat. No. 476619 ‡Units per case 210‡ Not available in Americas• MaxiSorp surface for binding of IgGand hydrophilic molecules• Can be used for dotting arrays directlyfrom MicroWell Plates• Same external dimensions as a96 MicroWell PlateLiterature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 11See Pages 195-196 for full reference list with titles and linksNunc-Immuno OmniTray,MaxiSorp SurfacePolystyreneCat. No. 465219External dimensions, mm 128 x 86Suggested working 35volume, mlUnits per pack/case 10/6078

www.nuncbrand.com - Always with updated informationImmuno Stick• Developed for ELISA and RIA tests awayfrom the laboratory environment• Gives reliable semi-quantitative resultswith built-in negative control• Application literature available• Choice of MaxiSorp or PolySorpsurfaces• The test system consists of a tube andstick with a paddle• Polypropylene tube for minimaladsorption of assay componentsImmuno AssayLiterature:Bulletin Nos: 6, 7, 8, 9See Pages 195-196 for full reference list with titles and linksA typical procedure with the Nunc-Immuno StickCoating of theMaxiSorp Stick with1 ml solution of aspecific antibodyBlocking with BSAIncubation of0.5 ml sampleIncubation in 1 mlenzyme conjugatedspecific antibodyIncubation withprecipitating enzymesubstrateRead result withnegative controlWashing inrunning tapwaterWashing inrunning tapwaterWashing inrunning tapwaterWashing inrunning tapwaterWashing inrunning tapwaterNunc-Immuno StickPolypropylene tubes. Polystyrene paddleCat. No. 475574 ‡ 472230 ‡External dimensions incl. lid, mm 49 x 12 49 x 12Paddle surface PolySorp MaxiSorpSurface area of the paddle approx., cm² 5.2 5.2Units per pack/carton/case 50/450/1800 50/450/1800‡ Not available in Americas79

Always with updated information - www.nuncbrand.comImmuno Tubes MiniSorpImmuno Assay• Ideal for liquid phase immuno assays,including RIA• The tubes are molded from a specialpolyethylene formulation• MiniSorp surface has proven loweravidity for proteins than glass and otherplastics• No blocking is required• Three tube sizes with stoppers available• Fit standard equipmentPercentage of added humanIgG adsorbed20%10%5%CPGMAdsorption of human IgG on:MiniSorp (M),Polystyrene (P),Glass (G), andCellulose nitrate (C)versus number of washings.Additions of 10 ug radio labeledHuman IgG to 50 µl saline prior towashing.Incubated 1 hour at 37°C followed byrepeated washing with 2 ml saline.12345Number of washingsNunc-Immuno TubesMiniSorp. PolyethyleneCat. No. 466982 ‡ 443990 468608External dimensions, mm 70 x 11 75 x 12 100 x 15Total volume, ml 4.0 5.0 12.0Graduation marking at ml 2.5 2.5 5 + 10Units per pack/case 150/3600 100/3000 150/1200‡ Not available in Americas80Stoppers forNunc-Immuno TubesPolyethyleneCat. No. 341866 ‡ 348801 343036Fits tubes with external 11 12 15diameter, mmUnits per pack/case 600/ 3600 500/ 3000 300/ 2400‡ Not available in Americas

www.nuncbrand.com - Always with updated informationImmuno Tubes PolySorp and MaxiSorp• Used for solid phase immuno techniques,e.g. IRMA, ELISA and ILMA• Available in MaxiSorp (hydrophilic) orPolySorp (hydrophobic) forms• Certified binding homogeneity• Nunc StarTubes with increased innersurface area give higher sensitivity andfaster assay times• High optical quality• Fits standard equipmentImmuno AssayLiterature:Bulletin Nos.: 1, 4, 6, 7, 8, 9, 10, 11, 12See Pages 195-196 for full reference list with titles and linksMolecules bound (arbitrary)200saturationsaturation1000050 100Incubation time (arbitrary)Average adsorption curves showingthe increase in number of boundmolecules and the decrease inincubation time obtainable with350 µl reactant volume by use ofthe 75 x 12 mm StarTube ( ),compared to the ordinary75 x 12 mm Tube ( ).The mutual relationship betweenthese curves holds for the bindingof every successive layer in theimmuno assay sandwich.Nunc-Immuno TubesPolystyreneCat. No. 475477 ‡ 476503 444474* ‡ 444202* 470319*Surface PolySorp PolySorp MaxiSorp MaxiSorp MaxiSorpConfiguration Standard tube StarTube Standard tube Standard tube StarTubeExternal dimensions, mm 75 x 12 75 x 12 70 x 11 75 x 12 75 x 12Total volume, ml 5.0 5.0 4.0 5.0 5.0Units per pack/case 100/3000 100/3000 150/1800 100/3000 100/3000* With certificate‡ Not available in Americas81

RPAAlways with updated information - www.nuncbrand.comMarked Products forAssistedReproductiveTechnologiesProducts forAssistedReproductiveTechnologiesTe

www.nuncbrand.com - Always with updated informationART/IVF IntroductionCertificationQuality in IVF means that our productsare manufactured according to theISO 13485:2003 standard by methodsdocumented in writing using validatedproduction equipment. In this way wecan ensure reproducibility within andbetween production lots.In order to be used for IVF, a productneeds regulatory approval (CE markingin Europe and 510(k) market clearancein the US). An independent approvalof the procedure, as well as the productused for any application, is required.Lloyds is among a few companies thatare certified by the EU commission toapprove a CE medical device marking.Such a company is called a notifyingbody. Lloyds is represented by thenumber 0088 and which appears withthe CE mark on Nunc products.The notifying body verifies that boththe development and the production ofthe product are in compliance with therelevant directive and fulfil the essentialrequirements. Important issues are“intended use of the product” and riskanalyses for both the production andthe usage of the product.roducts forThe vital criteria for an IVF productare that it is sterile and non-toxic.The directive recommendsperforming an appropriate test.Due to the importance of thecytotoxicity and the assumed smalltolerances, it is necessary to have fullbatch control of the raw material.ssistedThis to ensure that no variation incytotoxicity in the batch of polystyrenewill be transmitted. Several physicaleproductiveperformance tests are carried out onincoming raw materials, but there isa risk of cytotoxicity resulting fromA complete batch is irradiatedsimultaneously in order to minimizethe variation in the irradiation dose.Without full batch control therecould be a potential risk that the testresults achieved may not represent thecytotoxicity for all the products in theproduction lot.Each case of products contains acertificate with batch number printedon the back and the statements below:MaterialMaterial is crystalline virginpolystyrene, which has successfullypassed the USP biological reactivityclass VI-50° C test (7 days implant) andISO 10993 standards for cytotoxicity.SterilitySterility is obtained throughirradiation according to ISO 11137(sterilization of health care products- requirements for validationand routine control - radiationsterilization).Non-pyrogenicBatches are tested according to theprinciples of the LAL-test describedin the FDA guidelines and certifiednon-pyrogenic with a documentedendotoxin level of less than 20endotoxin units/device (0.5 endotoxinunits/ml) as stated in the USP.NEWNEWNEWContents1-cell Stage EmbryoToxicity Testing...................................... 84IVF 4 Well Dishes.....................................85IVF Petri Dishes Non-treated...............85ART/IVFmixing batches of raw materials.There is a similar issue with theirradiation process.chnologies83

Always with updated information - www.nuncbrand.com1-cell Stage Embryo Toxicity TestingART/IVFMouse Embryo Assay (MEA) test:Blastocyst formation ratio larger than80% for fully expanded blastocysts bothin test and control. The embryotoxicitytest is a release test and the productwill only be sold if it has passed the test.Description of the embryo toxicitytest for the 4 well dish 1-cell embryotoxicity testing.Indirect test: Extraction is done using0.5 ml “embryo-tested” (meaningpreviously tested) culture medium perwell for 48 hours at 37° C and 5% CO₂.The samples for 3 plates are pooled.Twenty-one 1-cell (B6C3F1xB6D2F1,i.e. a cross between strain B6C3F1female mice mated with B6D2F1male mice) embryos were cultured intriplicate in 12 µl drops of the resultingextract (previously embryo-testedmedium) supplemented with 0.4% BSA.Direct test: The embryos are cultureddirectly in the dish with 21 embryosin triplicate. Three of the 4 wells receiveseven 1-cell stage embryos in 0.5 mlof medium. All incubation is doneat 37° C and 5% CO₂. Performanceis observed at 24 and 96 hours.During the test, after 24 hours, thepreliminary result is obtained(the number of embryos that havedeveloped to the 2-cell stage isrecorded). After approximately 96 hoursfrom the time when the 1-cell stateembryos were added, the 4 Well Dishis removed from the incubator and theembryos are observed in a microscope.Only those embryos that aredetermined to have reached theexpanded blastocyst stage are scoredas being viable. The requirement forNunc IVF products is that at least 80%of the 1-cell test embryos must developto the expanded blastocyst stage within96 hours.The embryo toxicity test for theother products is a slight modificationof the test described above.84

www.nuncbrand.com - Always with updated informationIVF 4 Well DishesNunc IVF 4 Well DishesSterileCat. No. 144444 179830Description 4 Well Dish 4 Well DishNumber of wells 4 4Surface Nunclon Δ Non-treatedCulture area, cm²/well 1.9 1.9External dimensions, mm 66 x 66 66 x 66Units per pack/case 4/120 4/120IVF Petri Dishes Non-treated• Intended use is in vitro fertilization• CE marked according to MedicalDevices Directive 93/42/EEC• Produced and marketed in compliancewith the FDA demands for IVF products• Full batch control of all componentsin the final product for full traceability• Unique certification based on a1-cell stage mouse embryo toxicitytest; the certificate confirms:--Sterility (SAL 10-⁶)--Non-pyrogenicity--USP Class VI test• For MEA tested centrifugetubes (see Page 84)Accessories:Centrifuge Tubes Page 88CryoQuot Page 40EasyFlasks Page 11Literature:Tech Note No: 60See Pages 195-196 for full reference list with titles and linksFor further information visit: www.nuncart.comART/IVFNunc IVF Petri DishSterileCat. No. 150255 150270 150360Description Dish 35 x 10 Dish 60 x 15 Dish 90 x 17Number of wells 1 1 1Surface Non-treated Non-treated Non-treatedAirvent + + +Culture area, cm²/well 8.8 21.5 56.7External dimensions, mm 40 x 12 60 x 15 92 x 17Units per pack/case 10/500 10/400 10/15085

Always with updated information - www.nuncbrand.com

www.nuncbrand.com - Always with updated informationStorage & Handling IntroductionSafe handling of biological samples isvery important both for the personneland for the integrity of the samplesthemselves.The Nunc storage and handlingrange includes a series of single anddouble containers which comply withthe IATA regulations for transportof diagnostic and bio-hazardoussamples. This means that the tubes canwithstand evacuation to 95 kPascalwithout leaking. It is a requirementthat at least one of the transportcontainers fulfills this criterion. Thereare a number of different types of sealdesigns represented in this range.In keeping with our policy of makingstorage and transport containers for allmodern applications, we have extendedthe range of CE marked items. The 11ml polystyrene centrifuge tube nowjoins the CryoTubes and universalcontainers.The sputum container has beenredesigned. The new closure has beenimproved to make it much more userfriendly, especially efficient and easy touse in routine laboratories.NEWNEWNEWNEWNEWContentsCentrifuge Tubes 15 ml...............................88Centrifuge Tubes 50/200 ml.......................89EZ Flip Conical Centrifuge Tubes...........90Centrifuge Tubes 10/11 ml..........................91Urine Sample Kits.........................................92Sampling and Transport System..............93Sputum Containers......................................94Stor-It Tubes................................................95Storage Vials - Rack and Box.....................96Containers.....................................................97Disposable Tubes.........................................98Movettes.......................................................100Storage & HandlingMortar Kit.....................................................100Closure types:Plug Seal Screw CapRim Seal Screw CapDome Seal Screw Cap87

Always with updated information - www.nuncbrand.comCentrifuge Tubes 15 mlStorage & Handling• Available in polybutadiene styrene andpolypropylene• Printed volume graduationsalong length of tube with moldedgraduations at conical section• Meets USP Class VI• Flat cap and frosted area on tubeprovides writing surface for sampleidentification• Tube and cap suitable for freezing• Leak-proof closure• Polybutadiene styrene (PBS) tube hasexcellent optical clarity• PBS tube rated at 3,000 x g relativecentrifugal force (RCF), withstandstemperatures to 90°C• PBS tubes are non-pyrogenic• Polypropylene (PP) tube has excellentchemical resistance• PP tube rated at 7,800 x g RCF, withstandstemperatures up to 125°C• PP tubes are RNAse/DNAse freeDome Seal Screw Cap88Nunc Centrifuge TubesSterileCat. No. 366052 366036 366079 366060Material tube/cap PBS/HDPE PP/HDPE PBS/HDPE PP/HDPECap type Dome Dome Dome DomeTotal volume, ml 15 15 15 15Volume graduations Molded/ printed Molded/ printed Molded/ printed Molded/ printedExternal dimensions, mm 120 x 17 120 x 17 120 x 17 120 x 17Max. RCF tested 3000 7800 3000 7800Autoclavable - + - +Packing Racked Racked Loose LooseUnits per pack/case 50/500 50/500 50/500 50/500Caps are not autoclavable. Symbols: PBS = Polybutadiene styrene,PP = Polypropylene, HDPE = High Density Polyethylene, PE = Polyethylene

www.nuncbrand.com - Always with updated informationCentrifuge Tubes 50/200 ml373660• Fit most centrifuges• Tightness tested• Meet USP Class VIStorage & Handling374179339497377585Rim Seal Screw CapPlug Seal Screw CapNunc Centrifuge TubesCat. No. 334959 † 334940 † 373660 ‡ 373687 ‡ 339497 ‡ 342110 ‡ 376813*Cap type Rim Rim Rim Rim Plug - PlugTotal volume, ml 50 50 50 50 50 50 200Volume graduations Printed Printed Printed Printed Molded Molded MoldedExternal dimensions, mm 115 x 30 115 x 30 115 x 30 115 x 30 115 x 30 115 x 30 130 x 60Max. RCF tested 5000 5000 5000 5000 5000 5000 7000Sterile + + + + + - +Material tube/cap PP/HDPE PP/HDPE PP/HDPE PP/HDPE PP/HDPE PP/- PP/PEPacking Racked Bulk Racked Loose Loose Loose LooseUnits per pack/case 25/300 25/300 20/320 20/400 20/400 360/360 4/48Symbols: PBS = Polybutadiene styrene, PP = Polypropylene, HDPE = High Density Polyethylene, PE = Polyethylene.* RCF tested with adaptor 377585.‡ Not available in Americas / † Available only in AmericasAccessories for Nunc Centrifuge TubesCat. No. 342129 ‡ 377585 374179 347325 ‡DescriptionPlug seal screw capfor Cat.No. 342110Nylon cushion adaptorfor 200 ml tubesEpoxy/steel rackfor 6 pcs. 200 ml tubesUnits per pack/case 720/720 2/2 1/1 4/36Symbols: HDPE = High Density Polyethylene‡ Not available in AmericasExpanded polystyrenefoam rack for 20 pcs. 50 mltubes89

Always with updated information - www.nuncbrand.comEZ Flip Conical Centrifuge TubesStorage & Handling• Hinged-cap system• Ergonomic design for one-handedopening and closing• Integrated leakproof cap preventscross contamination and lostclosures• Clarified polypropylene combineschemical resistance and high speedtolerance• White writing area and flat caps forsample identification• Printed graduations• Standard dimensions compatiblewith centrifuge rotors90Nunc EZ Flip Conical Centrifuge TubesPolypropyleneCat. No. 362694 362695 362696 362697Volume, ml 15 15 50 50Packing Loose Racked Loose RackedMax. RCF tested 8500* 8500* 9500* 9500*Sterile + + + +Units per pack/case 50/500 50/500 25/500 25/500* When fully supported by conical rotor cavity or conical adaptor

www.nuncbrand.com - Always with updated informationCentrifuge Tubes 10/11 ml361883 362820 369740333510333510369738• Made in a variety of materials andformats for greater flexibility• Different cap types and bottom shapesto meet all needs• Fit most standard centrifuges• Relative centrifugal force tested (RCF)• Tightness tested• Polystyrene tubes are CE-Marked• See Pages 92-93 for transportcontainersStorage & Handling362308348224347856347885538318Rim Seal Screw CapNunc Centrifuge TubesPolycarbonate tubesPolypropylene caps (rim seal)Cat. No. 348097 ‡ 347759 ‡ 347708Bottom shape Round Conical ConicalTotal volume, ml 10 11 11External dimensions, mm 110 x 16 110 x 16 110 x 16Max. RCF tested 6000 6000 6000Autoclavable, tube/cap +/- +/- +/-Sterile + + +Writing area + - +Units per pack/case 80/480 80/480 80/480‡ Not available in AmericasPlug Seal Screw CapNunc Centrifuge TubesPolystyrene tubesPolyethylene caps (plug seal)CE-MarkedCat. No. 362308 ‡ 362707 ‡ 348224 ‡ 347856 ‡ 347880 ‡ 347885 ‡ 337846* ‡Bottom shape Flat Flat Round Conical Conical Conical ConicalTotal volume, ml 10 10 10 11 11 11 11External dimensions, mm 100 x 16 100 x 16 110 x 16 110 x 16 110 x 16 110 x 16 110 x 16Max. RCF tested 3000 3000 3000 3000 3000 3000 3000Volume graduations - - - - - + +Sterile - + + + + + +Writing area + + - - + + +Units per pack/case 80/480 80/480 80/480 80/480 80/480 80/480 80/480* Non-pyrogenic and 1-cell stage mouse embryo toxicity tested‡ Not available in AmericasNEWNEW91

Always with updated information - www.nuncbrand.comUrine Sample KitsStorage & Handling551314537745369738• Easy-to-use• Can be used by the patient at home• EAN barcode on the bag of product(Cat. No. 537745)• Transport and universal containerscomply with 95 kPascal test describedin UN Packaging Instructions 602/650for transport of infectious substancesand diagnostic specimens (except Cat.Nos. 333510, 333511 and 369740)• CE-marked538318Accessories:Transport containers Page 93Supersorp is a registered trademark of HuvecKlimaathbeheersingNunc Urine Sample KitsCE-marked. SterileCat. No. 551314 ‡ 537745* ‡ 538318 ‡ 369738 ‡DescriptionMaterial1 pc. 11 ml specimen tubewith conical bottom,writing area and screwcap; 1 pc. 100 ml sterilecup; 2 pcs. sterilemoistened napkinsTest tube: polystyreneUrine container:polyethylene1 pc. 11 ml specimentube with conical bottom,writing area and screwcap; 2 pcs. 100 mlcontainers; 6 pcs.cotton tampons; 10 mlphysiological salt waterTest tube: polystyreneUrine container:polyethyleneSampling Kit: 11 mlspecimen tube withscrew cap in bagPolystyreneSupersorp® absorbantmaterial for 10-12 ml fluid40 x 35 x 2 mmSupersorpUnits per pack/case 1/60 1/60 1/300 500/500* With instructions for home use (in Danish, English, German and Swedish)‡ Not available in AmericasNEW92Nunc Transport Containers and AccessoriesFor 10-11 ml centrifuge tubesCat. No. 361883 ‡ 362820 ‡ 333510 ‡ 333511 ‡ 369740 ‡ 369738 ‡DescriptionGrey transportcontainer withfoam insert333510 with capand containerseparatedTransparenttransportcontainer withfoam insertTransparenttransportcontainer,centrifuge tubeheld by thestopper333510 withSupersorp®absorbentmaterial incontainerMaterial container/cap HIPS/PE PP/PE PP/PE PP/PE PP/PE -Units per case 400 400 400 400 400 500HIPS = High Impact Polystyrene, PE = Polyethylene, PP = Polypropylene‡ Not available in AmericasNEWNEWNEWSupersorp®absorbentmaterial 10-12 ml40 x 35 x 2 mm

www.nuncbrand.com - Always with updated informationSampling and Transport System363282364246364238360577364882365048• Transport and universal containers havepassed the 95 kPascal test described inUN packaging instructions 602/650 fortransport of infectious substances anddiagnostic specimens• Safe handling and transport of blood,urine and fecal specimens• Inner and outer containers of highmechanical strengthStorage & Handling• Outer case available in transparent oropaque material369738• Universal containers are maximumrelative centrifugal force (RCF) tested• Caps designed to avoid dropletsadhering to the surface, reducing riskof aerosols• Inner containers available with a spoonfor handling of solid and semi-solidsamplesNunc UniversalContainer Screw CapNunc Transport Containersfor 25 ml Containersand Adsorbent MaterialCat. No. 364882 ‡ 365048 369738 ‡Color/Description Grey Transparent Supersorp® material for10-12 ml fluid40 x 35 x 2 mmMaterial container/cap HIPS/PE PP/PE -Units per pack/case 30/300 30/300 500/500NEWPS = Polystyrene, PE = Polyethylene, PP = Polypropylene, HIPS = High Impact Polystyrene, LDPE = Low Density Polyethylene‡ Not available in AmericasNunc Universal ContainersCE-MarkedCat. No. 364211 363282 364238 364246 360585 360577 ‡ 356790 †Spoon - - - - + + +Sterile - - + + + - -Working volume, ml 25 25 25 25 25 25 25Material container/cap PS/PE PS/PE PS/PE PS/PE PS/PE PS/PE PS/PEMax. RCF tested 4000 4000 4000 4000 4000 4000 4000Writing area - + - + + + -Units per pack/case 50/300 50/300 50/300 50/300 50/300 50/300 50/300PS = Polystyrene, PE = Polyethylene, HIPS = High Impact Polystyrene, LDPE = Low Density Polyethylene‡ Not available in Americas† Only available in Americas93

Always with updated information - www.nuncbrand.comStorage & HandlingSputum ContainersNEW• Low profile container can be shippedby normal mail• Containers comply with 95 kPascal testdescribed in UN Packaging Instructions602/650 for transport of infectioussubstances and diagnostic specimens• Large opening facilitates securesample placement• Lowered center of lid facilitatesscraping off samples when the lidis removed• The contents are well-protected fromdamage during transportation• Ribs on lid and container for easy grip.One container turned upside down canwork as an opening tool in analytic labsopening many containers• Special construction for removal of thesputum container from the transportcontainerA light pressure on the lid causes the sputumcontainer to tilt allowing it to be easilyremoved from the transport containerOne container turned upside downcan work as a opening toolConcave lid can be used to scrape sample if stuck tothe lid after the transportNEWNunc Sputum ContainersCat. No. 560623 ‡ 561077 ‡ 569746 ‡ 549263 ‡DescriptionSputum containerwith screw lidSputum container kit:Sputum container withscrew cap plus greytransport container withsnap capSputum container kit:Sputum container withscrew cap plus greytransport container withsnap cap and adsorbentmaterialTransport containerwith snap capExternal dimensions, mm 71 (dia.) x 23.5 80 (dia.) x 28.5 80 (dia.) x 28.5 80 (dia.) x 28.5Total volume, ml 24 - -MaterialContainer: PolystyreneScrew cap: PolyethyleneNEWTransport container forshipment: High impactpolystyreneSnap cap: PolyethyleneNEWTransport Container: Highimpact polystyreneSnap cap: PolyethyleneContainer: Highimpact polystyreneSnap cap: Polyethylene94Sterile + + + -Units per pack/case 8/192 5/100 5/100 150‡ Not available in Americas

www.nuncbrand.com - Always with updated informationStor-It Tubes• Free-standing tubes with starfoot foreasy handling• Caps and vials packed separately tosave handling time• One hand operation with starfoot tubesin Nunc CryoTube Rack(Cat. No. 376589)• Not recommended for use in the liquidphase of nitrogenStorage & HandlingStarfoot tube fitsinto grooves of theCryoTube Rack(Cat. No: 376589)- Page 47 - whichallows one-handoperation.Nunc Stor-It TubesExternal threadPolypropylene tubes with polyethylene caps.Non-sterileCat. No. 347651 347783 347791 347910 347929 348100Bottom shape Conical Round Round Conical Round RoundSuggested working 1.0 1.8 4.5 1.0 1.8 4.5volume, mlTotal diameter, mm 12.5 12.5 12.5 12.5 12.5 12.5Total length, mm 41 48 91 41 48 91Writing Area + + + - - -Starfoot + + + + + +Units per pack/case 500/2000 450/1800 300/1200 500/2000 450/1800 300/120095

Always with updated information - www.nuncbrand.comStorage Vials - Rack and BoxStorage & HandlingStorage Vials• Excellent for centrifugation and storageof PCR reagents, restriction enzymesand other diagnostic and biochemicalreagents or samples• Available in 0.5 ml and 2.0 ml sizes withfrosted writing area; 2.0 ml sizes havegraduations at 0.5 and 1.0 ml• Made of a High-Purity PolypropyleneCopolymer (PPCO) with excellentchemical resistance• The High Density Polyethylene (HDPE)screw closure is leakproof• Can be centrifuged to a maximumof 13,000 x g• Can be used from -40°C to 95°C• One full turn removes the ergonomicclosure - giving easy access to contentsNunc Storage VialsVial with closure. SterilePPCO Vials. Polyethylene screw capCat. No. 264262 264300Suggested working 0.5 2volume, mlTotal volume, ml 0.9 2.2Neck internal diameter, mm 8.4 8.4Height without closure 45.7 45.7Total height, mm 49 49Total diameter, mm 13 13Units per case 500 500At neck ring vial body is 10.2 mm.PPCO = High Purity Polypropylene CopolymerStorage box• (Cat. No. 264263) holds 100 of the0.5/2.0 ml vials in a 10 x 10 array andhas a clear lidRack• (Cat. No. 270972) holds 24 vials in a4 x 6 arrayWarningDo not use the vials for storage in the liquid phase ofliquid nitrogen. Such use may cause liquified nitrogento be trapped inside the vial and lead to pressurebuild-up resulting in possible explosion or biohazard.Nunc Storage Vial Box and RackBox/Rack for 0.5/2.0 ml VialsCat. No. 264263 270972Description Storage Vial Box Storage Vial RackMaterial Polycarbonate PolyketoneColor White BlueExternal dimensions, mm 144 x 144 x 62 146 x 92 x 55Tube array 10 x 10 4 x 6Units per case 12 4Accessories for Nunc Storage Vials - Color CodersPolystyreneCat. No. 264335 264408 264416 264440 264459 264467 264491Color White Yellow Orange Green Red Blue NaturalUnits per case 100 100 100 100 100 100 10096

www.nuncbrand.com - Always with updated informationContainers561301536668536455536323• A versatile range of containers• Made of high quality resins• Safe sample storage• Snap caps available for all containers• TransparentStorage & Handling536080536617536684Nunc ContainersPolystyrene. Non-sterileCat. No. 536080 536323 536455 536668 561301* 536757External dimensions, mm 29 x 27 (dia.) 41 x 27 (dia.) 60 x 27 (dia.) 49 x 41 (dia.) 54 x 53 (dia.) 54 x 53 (dia.)Total volume, ml 12.5 20 30 50 100 100Color Clear Clear Clear Clear Clear ClearUnits per pack/case 200/1000 100/2400 30/1200 40/800 1/100 300/300* Sterile, with capSnap Caps for Nunc ContainersPolyethylene. Non-sterileCat. No. 536617 536684 536889DescriptionSnap Cap for12.5, 20 and 30 mlcontainersSnap Cap for50 ml containersSnap Cap for100 ml containersColor Natural Grey GreyUnits per pack/case 200/4000 200/2400 150/4200NEW97

Always with updated information - www.nuncbrand.comDisposable TubesStorage & Handling• Relative centrifugal force (RCF) tested• Three different materials to suitmost needs• Polyethylene and polypropylene tubesare particularly useful in serologywhere the low adsorption of proteinsis of great importance• Polystyrene tubes are suitable fora broad range of applications• Polyethylene and polypropylene tubeshave good chemical resistance andhigh mechanical strength• Polystyrene tubes are transparent• For sterile work some tubes aresupplied with stoppers mountedAccessories:TubeRack Page 99Stoppers Page 99Nunc Disposable TubesCat. No. 349638 ‡ 341173* ‡ 361239 ‡ 341661 ‡ 341378 ‡Bottom shape Round Round Round Round RoundTotal volume, ml 2 2 3 4 4External dimensions, mm 38 x 12.5 45 x 12.5 55 x 11 70 x 11 50 x 13Max. RCF tested - - 3000 3000 3000Graduation marking at, ml - - 2.0 2.5 -Material tube/stopper PP/- PP/PE PS/- PS/- PP/-Sterile - + - - -Units per pack/case 500/2500 50/2000 150/3600 150/3600 150/3000Symbols: PP = Polypropylene, PE = Polyethylene, PS = Polystyrene.* Stopper mounted‡ Not available in Americas98Nunc Disposable TubesCat. No. 343923 341440 ‡ 342919 343141* 343001 ‡Bottom shape Round Round Round Round RoundTotal volume, ml 5 7 12 12 12External dimensions, mm 75 x 12 65 x 15 100 x 15 100 x 15 100 x 15Max. RCF tested 3000 - - - -Graduation marking at, ml 2.5 - 5 and 10 5 and 10 5 and 10Material tube/stopper PS/- PS/- PS/- PS/PE PP/-Sterile - - - + -Units per pack/case 100/3000 100/2200 150/1200 100/500 150/1200Symbols: PP = Polypropylene, PE = Polyethylene, PS = Polystyrene.* Stopper mounted‡ Not available in Americas

www.nuncbrand.com - Always with updated informationStorage & HandlingNunc Disposable TubesCat. No. 355581 ‡ 345608* ‡Bottom shape Conical ConicalTotal volume, ml 14 14External dimensions, mm 110 x 17 110 x 17Graduation marking at, ml 5 and 10 5 and 10Material tube/stopper PS/- PS/PESterile - +Units per pack/case 100/1800 100/500Symbols: PP = Polypropylene, PE = Polyethylene, PS = Polystyrene* Stopper mounted‡ Not available in AmericasNunc Tube RackFor Cat. Nos. 349638 and 341173Cat. No. 347155DescriptionMaterialNunc CryoTube Rackfor 70 tubesPolycarbonateExternal233 x 150dimensions, mmUnits per case 30/30Stoppers forNunc Disposable TubesCat. No. 341866 ‡ 348801 343036 345586 ‡Fits tubes with external 11 12 15 17diameter, mmMaterial PE PE PE PEUnits per pack/case 600/3600 500/3000 300/2400 300/ 1800Symbols: PE = Polyethylene.‡ Not available in Americas99

Always with updated information - www.nuncbrand.comStorage & HandlingMovettesNew to Americas• For semiquantitative liquid handling• Easy to handle• Strong suction capacity• Available in two sizes: 1.5 and 5 mlNunc MovettesPolyethyleneCat. No. 540673 550970 540657 550253Totalvolume, ml5 5 1.5 1.5Sterile - + - +Units perpack/case40/400 1/200 100/800 1/300Mortar KitNew to Americas• High impact polyethylene toleratestemperatures from -40°C to +95°C• Sterile, single packed• Includes scraper card, weighingpaper, polyethylene sheetNunc Mortar KitCat. No. 533669Description Mortar Kit, complete with greydish and pestleMaterial High Density PolyethyleneSterile +Units per 1/50pack/case100

Products forAssistedReproductiveTechnologieswww.nuncbrand.com - Always with updated informationVisit our websitewww.nuncbrand.comAlways up-to-date information on Nunc Brand Products.Our mission is toachieve completecustomer satis factionthrough qualityproducts and services.If you are looking for complete information on Nunc Brand Products, wehave a great resource for you:For the latest information on Nunc Brand Products – visit our website! Youwill find detailed product information, our entire product catalog, tips onselecting the right Nunc Brand Product for your application and expandedtechnical information, including:• Bulletins and Tech Notes• Protocols• Scientific posters and other literature• Specialized surfaces and resins• Application assistanceWe have provided electronic access to our customer service and technicalservice departments, links to our distributors and “news” to keep you upto-datewith our latest product developments.There is no better, quicker or easier way to look for information on NuncBrand Products. Visit us at www.nuncbrand.comCell Culture Cryogenics Immuno AssaysART/IVFStorage & HandlingProteomics/GenomicsART/IVFDrug DiscoveryDiagnostics (OEM)Nunc creates high quality labware for use in biotechnology, pharmaceutical andresearch laboratories, as well as in the production of vaccines and diagnostic kits.... And by the way! We also work in close cooperation with Nalgene

Always with updated information - www.nuncbrand.comProteomics & Genomics102

www.nuncbrand.com - Always with updated informationProteomics & Genomics IntroductionThis section includes products for Proteomic& Genomic applications suchas microbiology, PCR and molecularbiology.The ProPur range of products is advantageousfor the purification of antibodies(using Protein A or G columns)or fusion proteins (using IMAC) fromcell culture medium or ascites fluid.The NucleoLink surface is very versatileand binds DNA oligos covalently.The DIAPOPS technique is extremelyconvenient, allowing the user to performsolid phase PCR and detectionby hybridization in the same well; areplacement for conventional gel electrophoresisand Southern blotting.NucleoLink strips and plates areavailable in clear, black or whitepolymer and are compatible with allcommonly used thermal cyclers.For liquid phase PCR we offer a rangeof tubes and plates in the conventional96 well format. We also have 384 wellamplification plates.Also included in this section are samplehandling products for urine andsputum as well as a range of generallaboratory containers with snap caplids and serological tubes which nowjoin the universal containers.The Nunc inoculation loops are surfacetreated to hold the specified volume.A calibration certificate is available onrequest for any given production lot.All products are made using onlyvirgin raw materials. In many cases,certification and additional documentationis available.Accessories:As well as a number of holders andother practical items, we offer excellenttransfer systems for replication ofliquid phase as well as to membranes.The systems are designed for 96 wellbased systems and can be adapted forconversion to and from that format to384 wells.NEWContentsProPur Kits.................................................104Nucleolink Plates, Modulesand Strips.....................................................106TopYield Strips.........................................108Polypropylene Modules............................10996 Well Amplification Plates.................... 110384 Well Amplification Plates................. 111PCR Compatibility Chart.......................... 112Amplification Tubes and Strips .............. 114Amplification WorkstationTube Tray and Holder................................ 115Amplification Labtop Cooler................ 116MicroArray Slides MaxiSorpBlack and Clear Polymer.......................... 118MicroArray Slides NucleoLinkBlack and Clear Polymer.......................... 119MicroArray mSeries LifterSlip...............120Glass MicroArray Slides............................121Replication System....................................122OmniTray.....................................................123Bio-Assay Dishes........................................124Rectangular Dishes 1, 4 and 8 Well .......125Proteomics & GenomicsPetri Dishes..................................................126Loops and Needles.....................................127103

Always with updated information - www.nuncbrand.comProteomics & GenomicsProPur KitsLOADSPINNEW• All Mini and Midi column formats areavailable with three different surfacechemistries:--Recombinant Protein A for antibodies--Recombinant Protein G for antibodies--Metal chelate (IMAC) for His-taggedrecombinant proteins• The FlowGo® regulator maximizesthe contact time between the targetprotein and the resin matrix• ProPur kits have the speed of a spincolumn with the yield and purities of agravity column• ProPur Mini spin columns purify0.65 ml samples in under 20 min. usinga standard microfuge. ProPur Midispin columns purify 20 ml samples inunder 50 min. using a standard benchcentrifuge• Each ProPur Mini and Midi spincolumn is re-usable• Large volumes of dilute sample e.g. cellculture supernatant can be processedunder vacuum using ProPur Vac kitsLOCKRE-USENEW104Nunc ProPur KitsProtein A Spin ColumnsCat. No. 228100 228101 228102 228103 228104Description ProPur Kit Mini A ProPur Kit Mini A ProPur Mini A Bulk Pack ProPur Kit Midi A ProPur Midi A Bulk PackUltraFiltrationYes Yes No Yes Noconcentrators includedBuffers included All All No All NoUnits per case 2 16 48 4 12Buffers: A - binding buffer, B - elution buffer, C - neutralization bufferNEWNunc ProPur KitsProtein G Spin ColumnsCat. No. 228105 228106 228107 228108 228109Description ProPur Kit Mini G ProPur Kit Mini G ProPur Mini G Bulk Pack ProPur Kit Midi G ProPur Midi G Bulk PackUltraFiltrationYes Yes No Yes Noconcentrators includedBuffers included All All No All NoUnits per case 2 16 48 4 12Buffers: A - binding buffer, B1 and B2 - elution buffers, C - neutralization buffer

www.nuncbrand.com - Always with updated informationUse of peristaltic pump collar & sealing cap for loading largevolume sample onto the Midi Spin Column (Cat. No. 242002)NEWNunc ProPur KitsProtein A&G Spin ColumnsCat. No. 228110DescriptionProPur Mini Start A&GSealing CapPeristalticPumpPeristaltic Pump CollarSealing CapPeristaltic Pump CollarProteomics & GenomicsUltraFiltrationconcentrators includedNoBuffers includedNoUnits per case2A + 2GNEWNunc ProPur KitsMetal Chelate Spin Columns (IMAC)Cat. No. 228113 228114 228115 228116 228117 228118 228119DescriptionProPur KitMini MCProPur KitMini MCProPurMini MCProPurMini MCProPur KitMidi MCProPurMidi MCProPur Midi MCBulk packUltraFiltrationYes Yes No No Yes No Noconcentrators includedBuffers included All All All No All All NoUnits per case 4 24 24 72 8 8 24Buffers: A - binding buffer, B - elution buffer, C - neutralization bufferFiltered SampleNEWNunc ProPur KitsVacuum ColumnsCat. No. 228121 228122 228123 228124 228125 228126DescriptionProPur Kit Vac ACompleteProPur Kit Vac Aw/o Vac HeadProPur Vac ABulk PackProPur Kit Vac GCompleteProPur Kit Vac Gw/o Vac HeadProPur Vac GBulk PackUltraFiltrationYes Yes No Yes Yes Noconcentrators includedBuffers included Yes Yes No Yes Yes NoUnits per case 4 4 12 4 4 12Buffers: A - binding buffer, B1 and B2 - elution buffers, C - neutralization bufferNEWNunc ProPur Kit AccessoriesFor Protein A, G and Metal Chelate Spin ColumnsCat. No. 228111 228112 228120 242002Description ProPur ABuffersA: 250 mlB1&B2: 125 mlC: 30 mlProPur GBuffersA: 250 mlB: 125 mlC: 30 mlProPur MCBuffersPBS: 250 ml*Imidiazole:125 ml*ProPur PumpCollar, LoadingCap, PVCTubing (120cm (4 ft) of 3mm (1/8 in) ID)* Concentrated stock solutionNunc ProPur Kit AccessoriesFor Protein A and G Vacuum ColumnsCat. No. 228127 228128 242001DescriptionProPur VacHead, SampleLoading Capand Tubing (120cm (4 ft) of 3mm (1/8 in) ID)ProPur Vac Accessories,set of 3 replacementO-rings, connectors(6 mm, 8 mm) PVCTubing (46 cm (1.5 ft)of 6 mm (1/4 in) ID)ProPurPumpCollar105

Always with updated information - www.nuncbrand.comNucleolink Plates, Modules and StripsProteomics & Genomics• Covalent heat-stable binding of DNAAvailable in three formats:--C96 MicroWell Plate--Break Apart Modules--1 x 8 well StripsNucleoLink Plates and Modules:• Optimized for DNA hybridization assays• Available in transparent only• Break Apart format provides maximumflexibility• All modules available with color codingon requestNucleoLink Strips:The DIAPOPSTechniqueBPrimer OnePrimer TwoTemplateDetectionProbeDetection of Immobilized AmplifiedProducts using NucleoLink Strips ina One Phase System• Available in three colors:--Black for fluorescence assays--White for luminescence assays--Transparent for colorimetric assays• Optimized for Solid Phase PCR anddetection in the same well (DIAPOPS)--Ideal for hybridization assays1) BindingPrimer “One” isbound covalentlyto the surface of awell. For reasons ofsimplicity we havenamed the primers“One” and “Two”. Inthe actual assay the bound oligonucleotide canbe either the “upstream” or the “downstream”primer of an amplifica tion sequence.2) Additions ofReagentsBuffer, nucleo tides,Taq poly merase,Template, primer“One” and primer“Two” are added tothe liquid phase. Inthe liquid phase the ratio between primer “One”and primer “Two” should be 1:8.Features:• Sealable with heat-stable Tape 8• Thin walls (0.35 mm)• V-shaped wells with readable flatbottoms• Compatible with standard equipmentin MicroWell plate format includingmost 0.2 ml thermal cycler formats• Resists temperatures up to +121°C3) AmplificationAmplification is initiatedin the liquidphase. The ampliconsin the liquid phasewill hybri di ze withthe bound primermole cules. Theseprimer molecules will be extended by the Taqpolymerase.5) DenaturationAmplicons in theliquid phase areremoved by washing.The bound ampliconsare converted to single-strandedmolecules(denatured)by treatment with NaOH.4) Two types ofAmpliconAfter amplificationthe well contains twotypes of amplicon:Those in the liquidphase and thosebound to the well.6) DetectionThe single-strandedmolecules can bedetected by hybridizationwith a detectionprobe. When“Elisa-like” proceduresare used fordetection, the result can be read in a colorimetricor fluorometric 96 well plate reader.BDIAPOPS:• “ELISA-like” procedure combining solidphase PCR and detection by hybridizationin the same well• Replace conventional and timeconsuming detection methods suchas gel electrophoresis and Southernblotting• No transfer from amplification systemto detection system is necessary,reducing the risk of contaminationLiterature:Tech Note Nos: 17, 18, 19 Vol. 5., 36, 37See Pages 195-196 for full reference list with titles and links106

www.nuncbrand.com - Always with updated informationNucleoLink Plate 128 x 86 mmand Break Apart ModulesCat. No. 248150 ‡ 248450 ‡Description 96 well plate with C well Break Apart Modulewith 8 wellsColor Clear ClearTotal volume, µl/well 350 300Suggested working 150 150volume, µl/wellTotal surface area, mm² 122 127MaterialActivated heat-stablepolymerUnits per pack/case 5/30 5/30‡ Not available in AmericasActivated heat-stablepolymerNucleoLinkApplication Guideavailable on:www.nuncbrand.com/go/nucleolinkguideProteomics & GenomicsNucleoLink StripsCat. No. 248259 ‡ 248262 ‡ 248650Description Strip with 8 wells Strip with 8 wells Strip with 8 wellsColor Clear White BlackTotal volume, µl/well 330 330 330Suggested working 100 100 100volume, µl/wellTotal surface area, mm² 234 234 234MaterialActivated heat-stablepolymerActivated heat-stablepolymerActivated heat-stablepolymerUnits per tray/pack/case 12/120/1440 12/120/1440 12/120/1440‡ Not available in AmericasNucleoLink Frame for StripCat. No. 249182 ‡DescriptionFrame*ColorRedExt. dimensions, mm 128 x 86MaterialAcrylonitrile ButadieneStyreneUnits per pack/case 6/72* Heat-stable to max. 90°C‡ Not available in AmericasNucleoLink Starter KitCat. No. 249344 ‡DescriptionNucleoLink Starter KitContains48 NucleoLink Strips (clear),1 Frame, 60 Tape 8,1 Spacer Plate andApplication LiteratureUnits per pack/case 1/12‡ Not available in AmericasNucleoLink AccessoriesCat. No. 250105 ‡ 232702 259684*Description Tape 8 and Spacer Plate Amplification Tape 96 Spacer PlateExternal dimensions, mm 80 x 8.8 / 115 x 80 x 3 120 x 79 115 x 80 x 3Material Polyester Polyolefin SiliconeAdhesive Silicone Pressure sensitive acrylate -Units per pack/carton/case Tape 8: 60/480/5760Spacer Plate: 1/12100/100 1/1*Reusable and autoclavable‡ Not available in Americas107

Always with updated information - www.nuncbrand.comTopYield StripsProteomics & Genomics• Designed for liquid phase PCR orImmuno-PCR• Made of polycarbonate• Excellent protein binding• Excellent heat transmission• Comparable with 0.2 ml PCR tube• Compatible with most 0.2 ml thermalcycler formats• Sealable with heat-stable Tape 8Literature:Tech Note Nos: 18, 19 Vol. 5., 35See Pages 195-196 for full reference list with titles and linksbiotinylateddetectionantibodyartigencaptureantibodyMicroWellPlatestreptavidin - DNAoligomerImmuno-PCRdouble biotinylatedand hapten-labeledPCR-productantibodyenzymeconjugate*STV-coatedsurfacePCR-ELISA*) STV = StreptavidinImmuno-PCR detection of an antigen with subsequentquantitative analysis of product yields by PCR-ELISA.Capture antibodies are immobilized on MicroWellplates to bind the antigen selectively. Sequentialcoupling of a biotinylated detection antibody, streptavidin,and a biotinylated DNA marker assemblea signal generating immuno-complex. Signalamplification by PCR using a biotinylated primer anda digoxigenin-labeled nucleotide generates doublylabeled amplificate which can be quantified in aPCR-ELISA assay. The PCR pro ducts are immobilizedon streptavidin-coated plates and analyzed by antidigoxigeninIgG-alkaline phospatase conjugate witheither chromogenic or fluorogenic substrates.Nunc TopYield FrameCat. No. 249182DescriptionFrameColorRedExternal dimensions, mm 128 x 86Heat-stable to max. °C 90MaterialAcrylonitrile Butadiene StyreneUnits per pack/case 6/72Nunc TopYield StripsCat. No. 248909DescriptionTopYield StripsColorClearTotal volume, µl/well 330Suggested working 100volume, µl/wellTotal surface area, mm² 234MaterialPolycarbonateUnits per tray/pack/case 12/120/1440Nunc TopYield AccessoriesCat. No. 250105 ‡ 248917 ‡ 232702 259684*108DescriptionTape 8 andSpacer PlateTopYieldStarter Kit**AmplificationTape 96Spacer PlateExternal dimensions, mm 80 x 8.8 / 115 x 80 x 3 - 120 x 79 115 x 80 x 3Material Polyester - Polyolefin SiliconeAdhesive Silicone Pressure sensitive acrylate -Units per pack/carton/case Tape 8: 60/480/5760 Spacer 1/12** 100/100 1/1Plate: 1/12* Reusable and autoclavable** Per kit: 48 TopYield Strips, 1 Frame; 60 Tape 8, 1 Spacer Plate and Application Literature‡ Not available in Americas

www.nuncbrand.com - Always with updated informationPolypropylene ModulesOne white support rack is included in each case of green frames.• Ideal for sequencing, restrictionanalysis, transformations, ligationsand storage• Centrifugation eliminated whenmixing reagents• Easy well identification• Minimum binding of hydrophilicmolecules• Space saving at the bench and in thefreezer• Plastic waste is reduced• Tape and well design reduceevaporation• Maximizes heat transmission andsample recovery• Standard 96 well format• The modules are temperature resistantfrom -70°C to +121°CProteomics & Genomics“Shelf well” allowseasy mixing andsimultaneousaddition of reagentsand eliminatecentrifugation.Nunc Polypropylene ModulesNon-sterileCat. No. 232298 232034Well shape Round RoundTotal volume, µl/well 20 250Suggested working 10 100volume, µl/wellNumber of wells per 48 12moduleShelf well - +Color Natural NaturalUnits per pack/case 28/168 70/420Nunc FrameCat. No. 232042Units per pack/case 16/96Accessories Tape 12 and 48Polyester with silicone adhesiveCat. No. 232689* 232700*Description Tape 12 Tape 48External dimensions, mm 120 x 9 120 x 35Units per pack/case 100/400 100/400*Heat resistant from -20°C to 100°C109

Always with updated information - www.nuncbrand.com96 Well Amplification PlatesProteomics & GenomicsNunc 96 Well Amplification PlatesCat. No. 240600 230013* 267011** 230012 264605Description 96 Well 96 Well 96 Well 96 Well 96 WellAmplification Amplification Amplification Amplification AmplificationPlate without Plate with Plate with Plate with full Plate with fullskirtsemi skirt semi skirt skirtskirt, BlackMaterial240600230012230013264605267011Units per 5/50 5/50 5/50 5/50 5/50pack/case* Can be separated into 24 or 48 well sections** Fits the ABI-3730 and other new instrumentsPolypropylenePolypropylenePolypropylenePolypropylenePolypropylene• Plate, caps and tape are certified RNAseand DNAse free• Uniform thin wall construction of 0.2 mlwell offers excellent heat transfer withmaximum product yield• Rigid plate design without skirt fitsholder (Cat. No. 251357), allowing easymanipulation by automated handlingequipment• Conical wells of plate extend above thebase to permit sealing with strip caps orAmplification Tape 96 to prevent wellto well contamination• Strip caps have dome shape to preventcondensation and eliminate use of oiloverlays• Full skirted plate available in black forfluorescent applications• Silicone Spacer Plate ensures even heattransfer when Amplification Tape 96 isused• Amplification Tape 96 is offered insingle, precut sheets• Compatible with 96 well format thermalcyclers that hold an aluminum block for0.2 ml conical tubes• Compatibility table available• Flat, optically clear strip cap110Accessories for Nunc 96 Well Amplification PlatesCat. No. 259684* 265643 269080* 232702 235307** 276014 232698Description SpacerPlate1 x 8 AmplificationStrip CapsAmpli ficationTape 96AluminumTapeAluminumTape1 x 8 AmplificationStrip Caps, flat andoptically clearAdvancedPolyolefin Tape3M adhesivetechnologyMaterial Silicone rubber Polypropylene Polypropylene Polyolefin*** AdvancedPolyolefin***Adhesive - - - Pressuresensitiveacrylate.Units per pack/carton/case* Reusable and autoclavable** Superior optical properties for RT-PCR*** Resistant to temperatures from -70˚C to 110˚CPressuresensitive siliconeAluminumPressuresensitiveacrylateAluminum1/1 12/120 12/120 100/100 100/100 100/100 100/100Pressuresensitive acrylate

www.nuncbrand.com - Always with updated information384 Well Amplification Plates• Certified RNAse and DNAse free• The design and rigidity of the platesprevent distortion during PCR, makingthe plate easy and safe to handle• Raised well rims for effective sealingand reduced cross contamination risk• Cut away corners and alphanumericgrid-referencing• Standard footprint• 8 holes in the skirt facilitate platepositioning and removal from thethermal cycler block• Compatible with all leading 384 blockthermal cyclers (see Page 112)• Available in natural, black and whitefor colorimetric, fluorescence andluminescence based proceduresProteomics & GenomicsNunc 384 Well Amplification PlatesCat. No. 230582 230583 230667 240187 230584 264431Description384 WellAmplificationPlate384 WellAmplificationPlate384 WellAmplificationPlate384 WellAmplificationPlate with 1barcode label384 WellAmplificationPlate with 2barcode labels384 WellAmplificationPlate, AutomationCompatible (ABI3730 and others)Color White Black Natural Natural Natural NaturalMaterial Polypropylene Polypropylene Polypropylene Polypropylene Polypropylene PolypropyleneWorking volume, µl 2-30 2-30 2-30 2-30 2-30 2-40Total volume, µl 40 40 40 40 40 55Units per pack/case 10/50 10/50 10/50 10/50 10/50 10/50Accessories for Nunc 384 Well Amplification PlatesCat. No. 259684* 232702 235307** 276014Description Spacer Plate AmplificationAdvanced Polyolefin Tape Aluminum TapeTape 963M adhesive technologyMaterial Silicone rubber Polyolefin*** Advanced Polyolefin*** AluminumAdhesive - Pressure sensitive acrylate Pressure sensitive silicone Pressure sensitiveacrylateUnits per pack/carton/case 1/1 100/100 100/100 100/100* Reusable and autoclavable** Superior optical properties for RT-PCR*** Resistant to temperatures from -70˚C to 110˚C111

Always with updated information - www.nuncbrand.comPCR Compatibility ChartProteomics & Genomics230584Cat. No. 240600 230013 267011230012264605230582230583 264431Thermal CYCLERSABIGene Amp 2400Gene Amp 2700 YES YES YESGene Amp 9600 YES YES YES YESGene Amp 9700 YES YES YES YES YESBiometraUno YES YES YESUno II YES YES YES YESTpersonal CyclerT1 Thermocycler YES YES YES YES YEST3 ThermocyclerTgradient YES YES YESTrobot YES YES YES YES YESBio-RadGene CycleriCycler YES YES YES YESEppendorfMasterCycler Gradient YES YES YESErincompSingleBlock System YES YESTwinBlock System YES YESDeltacycler I YES YESThermoHybaidPCR Sprint YES YESPCR Express and Px2 YES YES YES YES YESmultiBlock System & mbs YES YES YES YES YESTouchdown YES YES YES YESOmnigene YES YES YESOmn-E YES YES YESMJ ResearchPTC- 200 DNA Engine YES YES YES YES YESPTC-225 DNA Tetrad YES YES YES YES YESPTC-100 w/96 well Block YES YES YESMWGPrimus 96 YES YES YESPrimus 384 YES YESStratagneRobocyclerYESTakaraTP 240TP 3000 YES YESTechneTouchgene Gradient YES YES YES YES YESFlexiGene YES YES YES YES YESGenius YES YES YES112

www.nuncbrand.com - Always with updated information230012264605230584230582230583 264431240600 230013 267011QPCR THERMAL CYCLERSABIGeneAmp 7500 YES YES YESPRISM 7000 YES YES YESPRISM 7700 YES YES YES YESPRISM 7900 HT YES YES YESBio-RadICycler YES YES YESStrategeneMX 4000 YES YESProteomics & GenomicsSEQUENCERSABIPRISM 3100 Genetic YES Adapter Req YES Adapter Req YES YESAnalyserPRISM 310 Genetic Analyser YES YESPRISM 3700 DNA Analyser YES Adapter Req YES Adapter Req YES YESPRISM 3730 YES YESPRISM 3730 XLYESMegaBACE500 YES1000 mark II YES4000 YES YESMJ ResearchBaseStationYESTransgenomicWave nucleic acidfragement analysis systemYESNunc Amplification Products113

Always with updated information - www.nuncbrand.comAmplification Tubes and StripsProteomics & GenomicsFAQWhat is the advantage of tubes over plates for PCR applications?Both formats work well as has been shown in Nunc Tech Notes No. 18 and 19(see Pages 197-198). Nunc Brand includes a variety of formats to provide enoughflexibility for PCR applications on any scale.• Certified RNAse and DNAse free• Optimized for liquid phase PCR• Made of virgin polypropylene whichcan withstand temperatures from-20°C to +121°C• Available in 0.2 ml strips or as 0.2 and0.5 ml individual tubes• V-shaped tubes with uniformly thinwalls• Offers uniform heat transmission formaximum yield• Dome-shaped caps for good contactwith heated lids of cycler• Flat caps for easy closure and samplelabeling• Compatible with most 0.2 ml or 0.5 mlthermal cycler formats• Flat, optically clear strip caps for realtimePCRAccessories:Tube Tray and Holder Page 115Amplification Labtop Cooler Page 116Nunc AmplificationTubes and StripsCat. No. 248161 250875 230895 230971 230885Description0.2 ml Tube Strips(8 tubes/strip withmatching cap strip)0.2 ml individualTubes with attacheddomed caps0.2 ml individualTubes with attachedflat caps0.5 ml individualTubes with attacheddomed capTotal volume, µl 200 200 200 500 500Suggested working 5 - 100 5 - 100 5 - 100 50 - 350 50 - 350volume, µlUnits per pack/case 12/120 500/1000 500/1000 250/1000 250/10000.5 ml individualTubes with attachedflat caps114Accessories for Nunc 0.2 ml Tube StripsCat. No. 269080* 265643Description1 x 8 AmplificationStrip Caps,flat and optically clear1 x 8 AmplificationStrip Caps,round topMaterial Polypropylene PolypropyleneUnits per pack/case 12/120 12/120* Superior optical clarity for RT-PCR

www.nuncbrand.com - Always with updated informationAmplification Workstation Tube Tray and Holder• Workstation for PCR set-up• Easy to assemble• Standard 96 MicroWell format to hold0.2 ml PCR tubes, strips or Nunc 96 WellAmplification Plate• Compatible with automated handlingsystems• Removable tray can be fitted directlyinto the thermal cycler• Compatible with V-bottom 0.2 ml tubeblock thermal cycler formats of majormanufacturers• Alphanumerically marked for sampleidentification• Tray fits holder in only one way to makeorientation easy• Can be used as a storage system• Stackable space saving units with lidlugs for stability during storage• Can be used from -20°C to +110°C• Chemically resistant to weak acids andalcoholsProteomics & GenomicsFrom Benchtop2. Insert stripor individualPCR tubesinto TRAY.Accessories:Amplification Plates Page 111Amplification 0.2 ml tubes/strips Page 1141. Place an assembled Tube Trayand Holder on the benchtop.to Thermal Cycler3. Adjust caps onthe tubes andpress into place.4. A full or partially loaded Tube Tray cannow be removed from the Holder andplaced in a thermal cycler block. Uponcompletion of PCR application, theTray slides back into the Holder.to Freezer5. The transparent Lid,covers the tubesin the Tray allowingstacking andfreezer storage.Nunc Tube Tray and HolderWith LidCat. No. 251357DescriptionExternaldimensions,mmMaterialUnits per case 1/5Tube Tray and Holder with LidTube Tray: 119.1 x 78.1 x 4.5Holder: 127.6 x 85.6 x 28.8Lid: 128.2 x 86.1 x 31.8Tube Tray: PolypropyleneHolder: PolypropyleneLid: Polystyrene (-40°C to 70°C)115

Always with updated information - www.nuncbrand.comAmplification Labtop CoolerProteomics & Genomics• Amplification Labtop Cooler protectsenzymes and solutions by maintainingfreezer temperature• Temperature is maintained between -20°C and -15°C during sample operation• Protects valuable reagents in thefreezer from fluctuations due to defrostcycles, open doors or power failure• The aluminum block holds up to 960.2 ml tubes in an 8 x 12 array• Compatible with 0.2 ml individualtubes and strips, 96 Well AmplificationPlate, NucleoLink Strips and TopYieldStrips and accommodates Nunc TubeTrayAccessoriesNucleoLink Strips Page 107Amplification 0.2 ml Tubes/Strips Page 114Tube Tray/Holder Page 115Nunc Amplification Labtop CoolerCat. No. 255500DescriptionAmplification Labtop CoolerMaterialPolycarbonate / AluminumUnits per case 1116

Products forAssistedReproductiveTechnologieswww.nuncbrand.com - Always with updated informationVisit our websitewww.nuncbrand.comAlways up-to-date information on Nunc Brand Products.Our mission is toachieve completecustomer satis factionthrough qualityproducts and services.If you are looking for complete information on Nunc Brand Products, wehave a great resource for you:For the latest information on Nunc Brand Products – visit our website! Youwill find detailed product information, our entire product catalog, tips onselecting the right Nunc Brand Product for your application and expandedtechnical information, including:Proteomics & Genomics• Bulletins and Tech Notes• Protocols• Scientific posters and other literature• Specialized surfaces and resins• Application assistanceWe have provided electronic access to our customer service and technicalservice departments, links to our distributors and “news” to keep youup-to-date with our latest product developments.There is no better, quicker or easier way to look for information on NuncBrand Products. Visit us at www.nuncbrand.comCell Culture Cryogenics Immuno AssaysART/IVFStorage & HandlingProteomics/GenomicsART/IVFDrug DiscoveryDiagnostics (OEM)Nunc creates high quality labware for use in biotechnology, pharmaceutical andresearch laboratories, as well as in the production of vaccines and diagnostic kits.... And by the way! We also work in close cooperation with Nalgene117

Always with updated information - www.nuncbrand.comMicroArray Slides MaxiSorp Black and Clear PolymerProteomics & Genomics• Polymer slide for protein microarrays• Ideal where very low concentrations areto be quantified• Low background fluorescence• Shelf life 5 years• Improved signal to noise ratio versusglass slides• Barcoded slides available on request• Instruction manual includedAccessories:mSeries Lifterslips Page 119mBox and Handle Page 1213500300025002000150010005000Hotline:microarray.nunc@thermofisher.com ormicroarray.nuncus@thermofisher.comThe figure shows a comparison of 11 different protein slides. The MaxiSorp Slide isthe best with regard to detection limits. Futhermore the MaxiSorp Slide has a very lowintrafield coefficient of variation (data not shown).Nunc MicroArray Slides MaxiSorpBlack and clear polymerCat. No. 230305 230302Method of attachmentNon-specific adsorption tohydrophilic surfaceKey applications Proteins ProteinsColor Black ClearUnits per pack/case 5/25 5/25Non-specific adsorption tohydrophilic surface118

www.nuncbrand.com - Always with updated informationMicroArray Slides NucleoLink Black and Clear Polymer• Preferred for covalent coupling ofnucleic acids• Immobilization of PCR productsand cDNAs by baking• Optimal for SNP’s applications• Preactivated surface, stableperformance for 5 years• Stripping and rehybridization• Low background fluorescence• Barcoded slides available uponrequest• Large batch size > 10,000• Instruction manual includedProteomics & GenomicsFluorescence signalShelf life study onNucleoLink surfaceThe graph shows thatNucleoLink surface can bestored for at least 49 monthswithout affecting its ability tobind nucleic acids. Furthermore,it is also shown thatsurfaces with immobilizednucleic acids can be storedfor a similar period withoutaffecting the subsequenthybridization.Accessories:mSeries Lifterslips Page 119mBox and Handle Page 121Day 1Stored for 49 monthsStored without immobilized nucleic acidsStored with immobilized nucleic acidsHotline:microarray.nunc@thermofisher.com ormicroarray.nuncus@thermofisher.comNunc MicroArray Slides NucleoLinkBlack and clear polymerCat. No. 230381 ‡ 230351 ‡Method of attachmentKey applications1. Covalent attachmentof nucleic acids, usingcarbodiimide (EDC)chemistry.2. Baking for cDNAs and PCRproductsNucleic acids, cDNAs,PCR productsColor Black ClearUnits per pack/case 5/25 5/25‡ Not available in Americas1. Covalent attachmentof nucleic acids, usingcarbodiimide (EDC)chemistry.2. Baking for cDNAs and PCRproductsNucleic acids, cDNAs,PCR products119

Always with updated information - www.nuncbrand.comMicroArray mSeries LifterSlipProteomics & Genomics• 1 mm thick glass• Uniform dispersal of hybridizationsolution• Reduced variation in hybridization fluidvolume• Consistent flatness• Excellent for microarray hybridizations• Other applications includein-situ hybridization andimmuno histo chemistryHotline:microarray.nunc@thermofisher.com ormicroarray.nuncus@thermofisher.comPrinted bars raise the LifterSlip slightly above the slide surface, creatingan ideal, highly reproducible hybridization environment.120Nunc MicroArray mSeries LifterSlipCat. No. 231647 ‡ 231650 ‡Material Glass GlassDimensions, mm 22 x 25 x 1 25 x 60 x 1Approx. volume, µl 20 55Units per pack/case 50/150 50/150‡ Not available in Americas

www.nuncbrand.com - Always with updated informationGlass MicroArray Slides• Multiple surfaces optimized for leadingmicroarray applications• Available 3D enhanced surface isetched for consistent, uniform spot size• Barcoding available for slide and datamanagement• Low fluorescence provides highsignal-to-noise ratio• High reproducibility due to consistentuniformity• Standard 25 x 75 mm dimensionProteomics & GenomicsHotline:microarray.nunc@thermofisher.com ormicroarray.nuncus@thermofisher.comAccessoriesmSeries Lifterslips Page 119mBox and Handle Page 121Compatible with commercial microarray spotting and detection systems, Nunc microarray substratesoffer consistent surfaces with reproducible spot size, shape and signal intensity.Nunc Glass MicroArray SlidesCat No. 231632 231633 ‡ 231638 ‡ 231642 231643 ‡ 231641 ‡Surface coating Aminopropylsilane Aminopropylsilane Aminopropylsilane Aldehyde Aldehyde EpoxySlide material Glass Glass Glass Glass Glass GlassEnhanced surface - - + - + +Barcode - + - - - -Method of attachment Non-specific, electrostatic adsorption Covalent, aminemodified DNAKey applications cDNA,PCR products, proteins Nucleic acids, SNPs, proteins Nucleic acids,proteinsUnits per pack/case 20/20 20/20 20/20 20/20 20/20 20/20‡ Not available in AmericasNunc Glass MicroArray SlidesCat No. 231639 ‡ 231636 ‡ 231644 ‡Surface coating Poly-L-Lysine Uncoated Gold thin filmSlide material Glass Glass GlassEnhanced Surface + + -Barcode - - -Method of attachment Non-specific, positive charge- -biological coatKey applications Proteins, cells Any glass coating processUnits per pack/case 20/20 20/20 20/20‡ Not available in Americas121

Always with updated information - www.nuncbrand.comReplication SystemProteomics & Genomics• For use with 96 and 384 Well Plate andOmniTray• Designed for the replication of DNAlibraries• Ideal for transferring small volumesof inoculum from plate to plate ormembrane (Cat. No. 250385)• OmniTray copier allows correctalignment of pins during replication• Pre-cut Pall Biodyne B nylon membrane(Cat. No. 250385) available to fitOmniTray• MicroWell copier alignment devicesimplifies conversion of 96 MicroWellplates to 384 well format• Flame-proof stainless steel pins on 384and 96 Pin Replicator• Pins transfer 1.0 µl from liquid or 0.1 µlto solid supports• TSP e.g. (Cat. No. 473245) is alsocompatible with OmniTray for makingreplicas or dot blotting. Transfervolume for PolySorp TSP is 1.0 µl/pinLiterature:Tech Note Nos: 21, 22, 23, 24See Pages 195-196 for full reference list with titles and linksAccessories:Immuno TSP Page 78OmniTrays Page 123122Nunc Replication SystemCat. No. 250393 250520 250539 250555 473245Description 384 Pin Replicator 96 Pin Replicator MicroWell PlateCopierOmniTray Copier Disposable 96 PinReplicator (TSP)Total volume, µl 1 ± 0.1 1 ± 0.1 - - -Sterile - - - - +External dim., mm H: 121.6*W: 83.3L: 135.4MaterialAnodized Aluminumwith stainless steelpinsH: 121.6*W: 83.3L: 135.4Anodized Aluminumwith stainless steelpinsH: 23.9W: 113.8L: 164.6H: 23.9W: 113.8L: 164.6H: 13.0W: 85.3L: 127.4Polypropylene Polypropylene Polystyrene,PolySorpUnits per pack/case 1/1 1/1 1/1 1/1 10/210* Height includes handle and pins

www.nuncbrand.com - Always with updated informationReplication System384 PinReplicatorReplication stepsReplication of libraries can be accomplished from:96 well to 96 well - 384 well to 384 well and96 well to 384 well - 384 well to 96 wellMicroWell Plate Copier384 WellSource Plate A384 WellPlateRepeat fromSource Plate BRepeat fromSource Plate CMicroWell PlateCopierDot blotting 4 each WellPlates onto one membrane384 PinReplicatorProteomics & GenomicsRepeat fromSource Plate DPlate AOmniTrayCopierClones initially plated on an OmniTraycan be picked by the hundreds with a384 Pin Replicator. This allows arrayingof as many as 1,536 clones.Biodyne BMembraneOmniTrayOmniTray• Ideal for screening libraries• Useful for DNA hybridization methods• Also suitable for standard petri dishtechniques and bacterial growthNunc OmniTrayPolystyrene, SterileCat. No. 242811 264728 250385DescriptionOmniTraywith lidOmniTraywith lidTotal volume, ml 90 90 -Suggested working vol., ml 35 35 -External dimensions, mm 128 x 86 128 x 86 115 x 76Units per pack/case 10/60 10/90 20/20Pall Biodyne Bnylon membraneActs as:--Holder for membrane when dot blotting--Container for denaturation,hybridization and washing steps--Storage container for membranes• Same external foot print dimensionsas a 96 MicroWell plate• Optical clarity makes colonieseasy to see• Can be stacked safely• Walls are high and sturdy• Can be frozen at -20°C123

Always with updated information - www.nuncbrand.comBio-Assay DishesProteomics & Genomics• Useful for screening large numbers ofcolonies in cloning experiments• Can be used for culturing of bacteriaand fungi• Suitable in agar diffusion assay• Can be used as a moisture chamber forincubation of Nunc MicroWell Plates• Lined with wet filter paper it is alsouseful as an incubation chamber for insitu hybridization• Large growth areaThe Low profile Bio-Assay Dish• Fits robotic instruments• Designed for genomic screening andcolony picking• Saves space9.8 mmStandard Height Dish243.9 mmLid21.9 mm236.7 mm229.0 mmDish10.3 mm16.8 mmLow Profile Dish240.8 mm233.4 mm226.5 mmLidDishNunc Bio-Assay DishesPolystyrene. Sterile. With lidCat. No. 240835 240845Description Standard Height Low ProfileExternal dimensions, mm 245 x 245 x 25 241 x 241 x 20Suggested working 225 215volume, mlTotal surface area, cm² 500 478Units per pack/case 4/16 5/20124

www.nuncbrand.com - Always with updated informationRectangular Dishes 1, 4 and 8 Well• Non-treated surface for molecular biologyapplications• ANSI standard dimensions for instrumentcompatibility1 Well• HTS Petri Dish applications4 and 8 Wells• Assay compartmentalization• Probe multiple gene sub-sets• Wash Western blot strips• 4 well dish holds four glass slidesProteomics & GenomicsRectangular Dishes, 1, 4 and 8 WellPolystyreneCat. No. 267060 267061 267062Surface Non-treated Non-treated Non-treatedNumber of wells 1 4 8Total volume, ml/well 90 22 11Color Clear Clear ClearSterile + + +With lid + + +Barcode - - -Units per pack/case 10/100 10/100 10/100125

Always with updated information - www.nuncbrand.comPetri DishesProteomics & Genomics• For culturing of fungi, bacteria andother microorganisms• Contact dish useful for sampling inhospital environments (for research useonly), as well as in food science and thepharmaceutical industry• Perform well in automatic dispensersdue to complete flatness and uniformheight• Available with a molded grid• Deep Petri Dishes allow for longerculture periods• Compatible with automated systemsNunc Lab-Tek Petri DishesPolystyreneCat. No. 4021 † 4022 † 4014 † 4031 † 4036 †Description Square Square Deep Petri Deep Petri Deep PetriExternal dimensions 100 x 17 100 x 17 150 x 26 100 x 26 63 x 22incl. lid, mmInternal dimensions, mm 89 89 137 86 57Culture area, cm² 79 79 148 57 25Stack ring - + - + +Airvent + - - + +Units per pack/case 20/500 20/500 12/72 12/300 16/400† Available only in AmericasNunc Petri DishesPolystyreneCat. No. 240401 249964 254925 263991External dimensions incl. 145 x 21 140 x 20 100 x 15 100 x 15lid, mmInternal dimensions, mm 136 x 18 136 x 18 86 x 12 86 x 12Total volume, ml 250 250 68 68Suggested working 35 35 12.5 12.5volume, mlVents + + + +Culture area, cm² 145 145 58 58Sterile - + - +Units per pack/case 10/180 10/80 20/320 20/320Nunc Contact DishesPolystyrene. SterileCat. No. 240541 ‡External dimensions incl. 67 x 15lid, mmInternal dimensions, mm 57 x 3Total volume, ml 7.7Suggested working 7.7volume, mlCulture area, cm² 25Units per pack/case 11/396‡ Not Available in Americas126

www.nuncbrand.com - Always with updated informationLoops and Needles• Meet semiquantitative standard forhandling samples• Surface treated for enhanced dropletadhesion• Non-toxic (USP Class VI)• Problem free inoculation with smoothloop surface• Precision molded• Calibration documents available for anybatch upon request• No flaming requiredProteomics & GenomicsUntreatedTNunc NeedlesPolystyrene. SterileCat. No. 253988 254399Description Yellow needle Yellow needleUnits per pack/carton/case 50/1000/4000 12/600/2400Nunc LoopsPolystyrene. SterileCat. No. 251586 254437 253287 254410Description Blue loop Blue loop Clear loop Clear loopTotal volume, µl 10 10 1 1Units per pack/carton/case 50/1000/4000 12/600/2400 50/1000/4000 12/600/2400127

Always with updated information - www.nuncbrand.comBio Production128

www.nuncbrand.com - Always with updated informationBio Production IntroductionThis section includes cell factories, 2DMicroHex microcarriers, roller bottles,as well as all associated accessories,manipulators and handling systems forboth laboratory and industrial scaleproduction.The flexible and cost efficient cellfactory system saves space, time andlabour and reduces contaminationrisk.The system is used world-wide forculturing and nursing cells used inproduction of drugs, and other bioactivecompounds as well as virus, cellpopulations and vaccines. The NuncLarge Scale Cell Culture system canprovide a total solution to productionat virtually any scale.The actively gassed cell factory forhigher yields and better control of thegrowth parameters is now joined byCell Factories EasyFill. With a wider,screw cap closure and a ‘plug and play’connector, the CF EasyFill makeslarger scale cell culture easier andmore accessible than ever.Nunc Brand can provide a custommade solution to large scale productionat any level from single units insmall laboratories to full scale massproduction. For more informationcontact us directly at:bioproduction.nunc@thermofisher.com.NEWNEWContentsCell Factories EasyFillNunclonΔ Surface...................................130Cell Factories NunclonΔ Surface.......... 131Cell Factories for Active GassingNunclonΔ Surface...................................132Nunc Cell Factory Instructions................133Cell Factories Accessories.........................134Cell Factory Flexible SystemCustom Capabilities..................................135Cell Factory Hand Manipulator CF40...136Automatic Cell FactoryManipulator ACFM.....................................136Nunc CO₂ Incubator...................................137In Vitro Roller Bottles.................................1382D MicroHex MicrocarriersNunclon∆ Surface....................................139Bio ProductionIncubator or incubation room? Ask us for advice on design and solutions- we have almost 30 years experience.129

Always with updated information - www.nuncbrand.comBio ProductionCell Factories EasyFill NunclonΔ SurfaceNEWA time and space saving vesselfor large scale cell culture production• Easy to fill and to empty• Ready to use, no accessories needed• Plug and play connection• One CF EasyFill-10 equals36 x T-175 flasksLiterature:For scientific references visit our website at:www.nuncbrand.com/go/easyfill1. Pour media directlyinto the CF EasyFill.NEWNunc Cell Factories EasyFill NunclonΔPolystyrene. Sterile. Length 335 mm, Width 205 mmCat. No. 140000 140250 140360 1404002. Equilibrate by placingthe CF EasyFill on theside with the small port.Number of trays 1 2 4 10Culture area, cm² 630 1260 2520 6300Suggested working vol., ml 200 400 800 2000Units per pack/case 1/6 1/6 1/4 1/2Options and Accessories3. Turn the CF EasyFill90°, so that the fillinginlet is up.Media will be separatedwith equal amounts ineach chamber.4. With filling inlet up, carefullytilt the CF EasyFillto a horizontal incubationposition. Handle byholdingon to the sides- do not manipulateby holding on to theupper rim.Fill or empty through the plug andplay connection, and work with asemi-closed system.AccessoriesWe offer a variety of devices (filters anda septum for sterile sampling, etc.) to fitthe plug ad play port. Please ask forcustom solutions.NEW5. Incubate.Cat. No. 173248 173249 173208 140099 132869DescriptionAirventfilter withconnector0.2 µ AirFilter withConnectorAirventfilter withconnectorand septumPC connectorlongClosure CapSterile + + + - +Units per 1/2 1/2 1/2 10/10 1/100pack/case6. Loosen filter capand remove cover cap.Empty directly intoreservoir.130

www.nuncbrand.com - Always with updated informationCell Factories NunclonΔ Surface• For industrial scale production ofvaccines, monoclonal antibodies orpharmaceuticals• Ideal for adherent cells• Can be used for suspension culture• Growth kinetics unaltered fromlaboratory scale culture• Available in 1, 2, 4, 10 and 40 trayversions for easy scale-up• Low contamination risk• Compact design• Certified NunclonΔ ensures consistentcell growth layer to layer, lot to lotBio ProductionFAQHow can the cell growth be monitored microscopically in Cell Factories?There are two ways.1st and 2nd tray from the bottom in an inverted microscope. This requiresa 4x objective with a working distance of approx. 25 mm. Such objectivesare available for most brands of microscopes. Contact the microscopesupplier for details. Alternatively, you can use a CF1 or CF2 as control.Literature:Tech Note Nos: 3 , Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksLarge scale animal cell cultivation for productionof cellular biologicals. Arne Johansson 1991.Available on requestAccessories:Accessories Page 134Cell Factory Hand Manipulator for CF40 Page 136Nunc Cell FactoriesPolystyrene. SterileLength 335 mm, Width 205 mmCat. No. 165250 167695 140004 164327 170009 139446Number of trays 1 2 4 10 10 40Culture area, cm² 632 1264 2528 6320 6320 25280Suggested working 200 400 800 2000 2000 8000volume, mlUnits per pack/case 1/8 1/5 1/10 1/2 1/6 1/2131

Always with updated information - www.nuncbrand.comCell Factories for Active Gassing NunclonΔ SurfaceBio Production• For industrial scale production of cellbased vaccines, monoclonal antibodiesor pharmaceuticals• The gas-flow system ensures acontrolled atmosphere in the culturetrays by equal distribution of userspecifiedgas-mix actively pumped• For culturing and nursing of pH sensitiveand high oxygen demanding cellsvia controlled gas distribution• Certified NunclonΔ surface treatmentensures consistent cell growth layer tolayer, lot ot lot• Growth kinetics unaltered from laboratoryscale cultureer 10• Available in 4, 10 and 40 tray versionsfor easy scale-up• Low contamination risker 7• Compact designer 4er 1er 10er 7er 4er 1Saturation (%)100806040Cell Factory vs Competitor (10 layer)Gas Distribution & Saturation at 0.5 l/mNunc Layer 1Nunc Layer 4Nunc Layer 7Nunc Layer 10Comp Layer 10Literature:Large scale animal cell cultivation for productionof cellular biologicals. Arne Johansson 1991.Available on request.Active Gassed Cell Factory Instructionfor Cell Factory CF4, CF10. CF40:www.nuncbrand.com/go/agcfinstructionsAccessories:Accessories Page 134Cell Factory Hand Manipulator for CF40 Page 1362000 10 20 30 40 50 60 70 80 90Comp Layer 7Comp Layer 4Comp Layer 1MinutesNunc Cell Factories forActive GassingLength 335 mm, Width 205 mmCat. No. 173239 173238 173240Number of trays 4 10 40Culture area, cm² 2528 6320 25280Suggested working 800 2000 8000volume, mlUnits per pack/case 1/10 1/6 1/2132

www.nuncbrand.com - Always with updated informationNunc Cell Factory Instructions1Remove seal fromadaptor cap5Turn 90 dregreesBio Production2Insert airvent filter6Place in horizontalposition3Connect to mediacontainer7Replace connector withcover cap. Incubate4Turn on its side,filter up.Allow media toequalize8Empty by reversingthe processLiterature:Cell Factory Instructionswww.nuncbrand.com/go/cfinstructions133

Always with updated information - www.nuncbrand.comCell Factories AccessoriesBio Production173208• Accessories for filling and emptyingthe cell factories with media173248173200173249179553140099167525167652170769Connectors, Filters and Caps for Nunc Cell FactoriesCat. No. 173248 173200 173208 179553 173249 167525 167652 170615 140082 140099 132869DescriptionAirventfilter withconnectorAirventfilter withconnectorAirventfilter withconnectorandseptumAirventfilter0.2 µAir FilterwithconnectorFilterAdaptorCapCovercapsCovercapsPCconnectorshortPCconnectorlongSterile + + + + + + + + - - +Units per 1/2 1/12 1/2 1/10 1/2 1/20 2/400 2/40 10/10 10/10 1/100pack/caseClosureCapStart-up KitSterileCat. No. 170769Description Starter kit includes: PC Connector,Airvent Filter, White Filter AdaptorCap, Cover Caps, Tube Clamp andSilicone TubingCap for Cell Factory EasyFillCat. No. 132869Closure Closure CapUnits per 1/100pack/case134

www.nuncbrand.com - Always with updated informationCell Factory Flexible System Custom CapabilitiesCustom Solutions-Ask us what we can do for you!Do you have a preferred type of tube, connection or have youalready validated a specific filter? No problem! We are very flexibleand can assist you in designing the optimal configuration.Pour, plug, click or weld you access to our system:Bio ProductionPlease contact bioproduction.nunc@thermofisher.comfor additional information or to get started.PLUG a media container into our standard port via a standardcell factory connector. Provides a semi-closed system for GMPproduction.POUR into our CF EasyFill - easy filling and emptying withoutany accessories needed to get started.Recommended for research and process development.WELD the pre-assembled heat sealed tube of yourchoice together with your media vessel. A closedsystem that allows you to work outside a laminar flow hood.CLICK a CPC connector on your media bag into a maleCPC port. A semi-closed system for GMP production.135

Always with updated information - www.nuncbrand.comCell Factory Hand Manipulator CF40Bio Production• The Nunc Hand Manipulators aredesigned to manually handle aCell Factory 40 with a growtharea of 25,280 cm²• The CF40 Hand manipulator comeswith wheels and a foot brakeCF40 with a growth area of 25,280 cm².CF40 Hand Manipulator (Cat. No. 176953).Nunc Cell Factory HandManipulator Handling SystemCat. No. 176953DescriptionMaterialCF40 Hand ManipulatorStainless steelFor Cell Factory Cell Factory 40Cat. Nos. 139446 and 173240Automatic Cell Factory Manipulator ACFM• The ACFM is an electronically andpneumatically controlled unit whichautomates the filling and emptyingof medium or cell suspension into4 x 3 Nunc Cell Factory 10 (Cat. Nos.164327, 170009 and 173238) or 4 x 1Cell Factories 40 (Cat. Nos. 139446 and173240)1 assembly rack with rack carts for 4 Nunc Cell Factories, CF40Nunc Automatic Cell Factory Manipulator ACFMCat. No. 132744DescriptionAutomatic CF manipulatorMaterialStainless steelFor Cell Factory 10 Cat. Nos. 164327, 170009, 173238, 140400For Cell Factory 40 Cat. Nos. 139446, 173240136

www.nuncbrand.com - Always with updated informationNunc CO₂ Incubator• Doors with heated windows andblinders• CO2 control• Temperature control• Internal fans improving air circulation• All alarm messages stored until activelycleared by operator• Acoustic alert and alarm signal• Available the data logger; FDA 21 CFRpart 11 compliant• Available with gas distribution manifoldfor active gassing• Available with controller/mixer of up to3 gasses for active gassing• Racks for 4x3 CF10 with pre-mountedmanifold for active gassingBio Production°C3938,53837,53736,536Temperature Mapping Empty Incubator0 12 24 36 48 60HoursA, front, topE, back, topA, back, bottomA, back, topB, middle, bottomE, middle, middleD, middle, bottomA, front, bottomC, back, topB, middle, topC, front, bottomD, middle, topC, back, bottomF, front, bottomA, middle, middleC, front, topF, front, topE, back, bottomF, back, topE, front, bottomF, back, bottomF, middle, middleE, front, topAverageNunc IncubatorTemperature up to 60° CCat. No. 140224MaterialStainless steelFor Cell Factory 10 Cat. Nos. 164327, 170009, 173238For Cell Factory 40 Cat. Nos. 139446, 173240Incubator Width 1800 mmDepth 1190 mmHeight 1610/2430 mmWeight 600 kgTemperature Up to 60°CO₂ Nom. Value: Up to 20%137

Always with updated information - www.nuncbrand.comIn Vitro Roller BottlesBio Production• For industrial scale productionof vaccines, monoclonal antibodiesor pharmaceuticals• Molded of durable PETG• Excellent substrate for adherent cells• Quick-action ergonomic closurereduces wrist strain and increasesproductivity• Wide range of sizes with surfaceareas from 1050 cm² to 4200 cm²• Available in both Standard andExpanded Surface (XPS), that enablesincreased cell growth• and product yield without the need to• purchase additional productionequipment• or increase labor• Easy-to-read graduations formedium fills• Lot number is printed on each bottleto maximize traceabilityLiterature:Tech Note Nos: 3, 25, 57See Pages 195-196 for full reference list with titles and linksNunc In Vitro Roller BottlesPETG. SterileCat. No. 1060-05 1060-20 1060-52 1060-50 1060-85 1860-22 1760-20 2160-05 2160-20 4260-22NEWDescription Standard Standard StandardPDL-CoatedNEWStandardPDL-CoatedStandardVentedStandardLongExpandedSurfaceExpandedSurfaceExpandedSurfaceExpandedSurfaceSize 1.2X 1.2X 1.2X 1.2X 1.2X 1XL 2X 2.5X 2.5X 5XCulture area, cm² 1050 1050 1050 1050 1050 1800 1700 2100 2100 4200Suggested working 100-500 100-500 100-500 100-500 100-500 200-1000 200-600 200-600 200-600 400-1000volume, mlUnits per pack/case 5/20 20/20 2/2 20/20 5/20 22/22 20/20 5/20 20/20 22/22AccessoriesReplacement ClosuresCat. No. 3080-01Description48 mm HDPE Vented Quick-Action ClosureSterile +Units per pack/case 1/300138

www.nuncbrand.com - Always with updated information2D MicroHex Microcarriers Nunclon∆ Surface• 2D MicroHex are solid, non- swelling,non-porous and non-absorbingmicrocarriers for adherent cell culture• Certified NunclonΔ surface ensuresexcellent conditions for cell attachmentand growth• 2D MicroHex can be kept in suspensionat low stirring speed due to the shapeand low weight of the particles, resultingin a uniform coverage of all carriers• Very low displacement volume/unitsurface areaBio ProductionLiterature:Tech Note No: 48See Pages 195-196 for full reference list with titles and links125 µ25 µRegular hexagon:Side length = 125 µThickness = 25 µDensity = 1.05 g/cm³Cultivation area = 760 cm²/gRadiation sterilizedSolid particlesNon-porousNon-absorbingNon-swelling in aqueous mediaNon-toxicNunc 2D MicroHex Microcarriers NunclonΔSterileCat. No. 139102 139104 139106 139108Grams pr. bottle 2 10 20 100Culture area, cm² 1500 7500 15000 75000Units per case* 10 5 5 2Custom-made packaging, available on request.139

Always with updated information - www.nuncbrand.com

www.nuncbrand.com - Always with updated informationPlates IntroductionContentsBased on decades of experience, NuncBrand provides a very wide range ofplates. In addition to 96 well plates, wealso provide 384 and 1536 well formatswith both solid and optical bottoms inpolystyrene (primarily for assays) andour wide range of polypropylene andDeepWell plates both for assayand storage applications.Polystyrene platesThe polystyrene range offers plateswith flat bottom (F), conical (V),round (U), or C-shaped bottom(flat in the center with rounded cornersfor efficient washing) most of whichare available in clear, black or white.We also offer both a polymer bottomand a cover glass optical bottom platefor applications where viewing frombelow is necessary. In cell culture, newPoly-D-Lysine and Collagen I coatedversions are now available. Unique tothe market are our CC2 glass basedplates treated with a chemical coating,which mimics polylysine.The range of Immuno-plates hasbeen expanded from the MaxiSorp,PolySorp, MediSorp and MultiSorpto include Immobilizer Glutathione,Ni-Chelate and Immobilizer Amino,which have joined Nunc CovaLink NHin our range of actively binding plates.the plate is used maximized. Since ourV-shaped 0.45 ml and U-shaped 0.5 mlplates are available in white or black,they are also suitable as assay plates.For easy identification the 0.5 and 0.45ml polypropylene plates are also madein blue, yellow and red.All the polypropylene plates aresuitable for heat sealing and theapplication of sealing tapes.Plate materials are also optimizedfor diverse applications. MicroWellplates are made using polystyrene (PS)or polypropylene (PP). Polystyrene ischaracterized by its optical clarityand can be modified to give differentbinding characteristics, likeNunclonΔ for cell culture andMaxiSorp for IgG adsorption.As polypropylene is resistant tochemicals and temperature extremes,it is ideal for storage of chemical compounds.For many applications it isalso considered as a better choice forstorage of biomolecules and as a platefor homogeneous (liquid phase) assays.NEWOverview of Nunc Plates..........................142Application Information..........................143F96 MicroWell PlatesPolystyrene Clear.......................................145F96 MicroWell PlatesPolystyrene Clear .......................................146F96 MicroWellPlates Polystyrene Black and White.......147Immobilizer F96 MicroWell PlatesPolystyrene Black and White...................148C96 MicroWell Plates Polystyrene.......149U96 MicroWell PlatesPolystyrene Clear.......................................150V96 MicroWell PlatesPolystyrene Clear .......................................15196 Well Optical Bottom PlatesPolymer Base...............................................15296 Well Optical Bottom PlatesPolystyrene/Coverglass............................153U96 MicroWell Plates Polypropylene...154V96 MicroWell Plates Polypropylene....155V96 MicroWell Plates Polypropylene....156U96 DeepWell Plates1.3 ml & 2.0 ml Polypropylene.................15796 DeepWell Plates1.0 ml Polystyrene......................................15896 Filter Plates 1.3 ml.................................159Immobilizer 384 Well PlatesPolystyrene..................................................160384 ShallowWell StandardHeight Base Polystyrene...........................162384 ShallowWell Plates Polystyrene......163384 Well Optical Bottom PlatesPolystrene/Polymer Base.........................164384 Well Optical Bottom PlatesPolystyrene/Coverglass Base....................165384 Well Plates Polypropylene................166384 ShallowWell StandardHeight Plates Polypropylene...................167384 DeepWell Plates Polypropylene......1681536 Well Plates Polystyrene...................1691536 Well High Base Plates Polystyrene... 170Well Cap Mats.............................................171Microplate Storage Racks........................172Overview of Nunc Lids.............................. 174Nunc Lid Guide...........................................175Sealing Tapes..............................................176PlatesPolypropylene platesMost of the Nunc 96 well polypropyleneplates feature the shared walltechnology, where the internal space inNEWAbbreviation Description Advantages or Primary applicationF Flat Bottom For assays where a flat bottom is needed foroptical or application reason e.g. cell cultureU Round Bottom For optimal washing effect and mixing orwhere a preci pitate is to be examinedCRounded corners& Flat BottomThe rounded corners allow efficient washing withthe optical advantages of the flat bottomed wellVPS OpticalBottomCG OpticalBottomConicalBottomPolystyreneOptical BottomCoverglassOptical BottomFor full plate drawings, see www.nuncbrand.comFor precipitation or in order to optimizerecovery of small volumesFor applications e.g. chemiluminescence/fluorescencewhere a colored upper structure is neededin combination with a clear bottomFor applications e.g. chemiluminescence/fluorescencewhere a colored upper structure is needed incombination with a clear thin bottom with superioroptical properties e.g. confocal microscopyNEWNEWNEWNEW141

Always with updated information - www.nuncbrand.comPlatesOverview of Nunc PlatesCell CultureCC²Poly-D-LysineCollagen IAminoStreptavidin passively coatedStreptavidin covalent coatedGlutathioneNickel-ChelateNucleoLinkPolySorpMediSorpMaxiSorpMultiSorpNon-treated, non-sterileNon-treated, sterileFormat Polymer Type Color Bottom Volume (µl/well)Flat (F) 400 + + + + + + + + + + + + +Flat w/curved edges (C) 350 + + + +ClearRound (U) 300 + + + + +SolidConical (V) 300 + +Flat (F) 400 + + + + + + + + +WhitePolystyreneFlat w/curved edges (C) 350 + +Black Flat (F) 400 + + + + + + + + +Flat (F), Polystyrene base 400 + + + +WhiteFlat (F), Coverglass base 400 + +96OBP*Flat (F), Polystyrene base 400 + + + +MicroWellBlackPlatesFlat (F), Coverglass base 400 + + +NaturalRound (U) 500, 1300, 2000 + +Conical (V) 300, 450 + +WhiteRound (U) 500 +Conical (V) 450 +Polypropylene SolidRound (U) 500 +BlackConical (V) 450 +Red, Round (U) 500 +Blue,YellowConical (V) 450 +ClearFlat (F) 120 + + + + + + + + +Flat (F), ShallowWell ** 25 + + +Solid WhiteFlat (F) 120 + + + + + + + +Flat (F), ShallowWell ** 25 + +PolystyreneFlat (F) 120 + + + + + + + +BlackFlat (F), ShallowWell ** 25 + + +White Flat (F), Polystyrene base 120 + +384OBP*Flat (F), Coverglass base 120 + + +BlackWellFlat (F), Polystyrene base 120 + + + +PlatesRound (U) 120, 252 + +NaturalRound (U), ShallowWell ** 58 + +Round (U) 120 +Polypropylene SolidWhite Round (U), ShallowWell ** 58 +Round (U), Low CrossTalk 120 +BlackRound (U) 120 +Round (U), ShallowWell ** 58 +Blue Round (U) 120 +1536Clear Flat (F) 13 + +Well Polystyrene Solid White Flat (F) 13 + +PlatesBlack Flat (F) 13 + + +* OBP = Optical Bottom Plates** ShallowWell = small volume platesWell ColorClear (PS)Natural (PP)White (PS or PP)Black (PS or PP)Black, white, blue yellow or red (PP)ApplicationsColorimetric assaysStorageLuminescenceFluorescence, LuminescenceEasy identification of different series of platesTechnical drawings of all plates can befound on Nunc Brand’s Plate Guide:www.plateguide.com142

www.nuncbrand.com - Always with updated informationApplication InformationSurface area, liquid height and liquid volume dataIn recent years many new plate formatshave been introduced.Dense plate formats, such as 384 and1536 MicroWell plates, are being usedto decrease assay volume and handlingtime. However, those formats aredifficult to handle in the initial manualassay development. In assay development,tests are often made in simplerformats, such as 96 MicroWell plates.After development, the assays aretransferred to the denser formats.Most of the time the transformationis very straightforward, but the resultscan deviate from the originaldevelopment results.This may be due to the difference insurface to volume ratio as the kineticsof a solid phase assay can change inthe smaller wells. The liquid height isa relevant factor for OD, but may alsoplay a role in homogeneous assays.We hope to help you in the shiftbetween different plate formats withthe information on the followingpages. If you have additional questionsor need more information, please donot hesitate to contact us.PlatesLiquid height (H) vs. liquid volume (V)H mm121086F96 MicroWell plate.Pinchbar and OpticalBottom Plate designF96 MicroWell plate.Straight flange designU96 MicroWell plateC96 MicroWell plate4200 50 100 150 200 250 300 350 400V µl12Liquid height (H) vs. liquid volume (V)11109F96 MicroWell plate384 MicroWell plate384 ShallowWell plate1536 MicroWell plate87H mm65432100 25 50 75 100 125V µl143

Always with updated information - www.nuncbrand.comApplication InformationPlatesS mm 2300250200150Covered surface area (S) vs. liquid volume (V)F96 MicroWell plate.Pinchbar and OpticalBottom Plate designF96 MicroWell plate.Straight flange desi nU96 MicroWell plateC96 MicroWell plate150F96 MicroWell plate384 MicroWell plate125384 ShallowWell plate1536 MicroWell plate10075S mm 2Covered surface area (S) vs. liquid volume (V)10050502500 50 100 150 200 250 300 350 400V µl00 25 50 75 100 125V µl3,0Surface to volume ratio (S/V) vs. liquid volume (V)Surface to volume ratio (S/V) vs. liquid volume (V)3,02,52,52,0F96 MicroWell plate384 MicroWell plateF96 MicroWell plate384 MicroWell plate3,53,0384 MicroWell plate384 ShallowWell plate1536 MicroWell plateS/V mmS/V mm 2 /µl2 /µl2,01,51,51,01,00,50,50,00 50 100 150 200 250 300 350 4000,0V µl0 50 100 150 200 250 300 350 400V µlS/V mm 2 /µl2,52,01,51,00 5 10 15 20 25V µlSurface/volume ratio (S/V) vs. liquid height (H)43F96 MicroWell plate384 MicroWell plate384 ShallowWell plate1536 MicroWell plateS/V mm 2 /µl2100 2 4 6 8 10 12H mm144

www.nuncbrand.com - Always with updated informationF96 MicroWell Plates Polystyrene Clear• MaxiSorp, MediSorp, MultiSorpand PolySorp for solid phaseimmuno assays• NunclonΔ, Collagen I andPoly-D-Lysine for cell culture and assays• Available pre-labeled with barcodes• Clear plates with excellentoptical clarity• Working volume range:50 - 250 µl/wellPlatesmmTotal height of plate 14.4 ± 0.2Literature:Bulletin Nos: 1, 2, 4, 6, 7, 8, 9, 10, 11, 12, 15See Pages 195-196 for full referencelist with titles and linksHigh Flange DesignH4H3D1D2F1H2H6H2 Vertical distance from inside 3.0well bottom to resting planeH3 Inside depth of well 11.4H4 Vertical distance from external 1.9well bottom to resting planeH5 Height of flange on the short side 2.4H6 Height of flange on the long side 7.4D1 Well diameter, top 7.0D2 Well diameter, bottom 6.2F1 Depth of flange 1.7Nunc MicroWell PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Barcode Units per pack/case156545 * Cell Culture Clear 400 + - - 10/180161093 * Cell Culture Clear 400 + + - 10/160167008 * Cell Culture Clear 400 + + - 1/50168055 * Cell Culture Clear 400 + - - 1/50152038 Collagen I Clear 400 - ** + - 5/20152039 Poly-D-Lysine Clear 400 - ** + - 5/20256510 ‡ Non-treated Clear 400 + - - 10/180260836 Non-treated Clear 400 - - - 10/180260844 Non-treated Clear 400 - - + 10/180260860 Non-treated Clear 400 + + - 10/160260887 Non-treated Clear 400 + + + 10/160260895 Non-treated Clear 400 - + - 10/160456529 PolySorp Clear 400 - - - 10/180456537 * MaxiSorp Clear 400 - - - 10/180460984 * MaxiSorp Clear 400 - - + 10/180467320 * MediSorp Clear 400 - - - 5/60467340 * ‡ MultiSorp Clear 400 - - - 5/60* Certified** Produced in clean environment‡ Not available in Americas145

Always with updated information - www.nuncbrand.comF96 MicroWell Plates Polystyrene ClearPlates• MaxiSorp and PolySorp surfacesfor solid phase immuno assays• Non-treated surfaces forscreening assays• Working volume range: 50 - 250 µl/wellImmobilizer:• Covalent attachment• High sensitivity and specificity• High signal to noise ratio• Amino surface for rapid couplingof peptides, proteins and DNA• Streptavidin surface for rapid couplingof biotinylated biomolecules such aspeptides, antibodies, oligonucleotidesor haptens• Glutathione surface for rapid coup l ingof GST-tagged fusion proteinsF1D1H3mmTotal height of plate 14.5 ± 0.2H2 Vertical distance from inside 3.2well bottom to resting planeH3 Inside depth of well 11.2H4 Vertical distance from externalwell bottom to resting plane2.1• Nickel-Chelate surface for rapid couplingof 6x Histidine-tagged proteinsLiterature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10, 11, 12,Tech Note Nos: 42, 43, 44, 45, 46, 47, 56See Pages 195-196 for full reference list with titles and linksH5H4D2H2H5 Height of flange 2.85 ± 0.15D1 Well diameter, top 7.05 ± 0.2D2 Well diameter, bottom 6.5F1 Depth of flange 2.0Accessories:Racks Page 172Sealing Tapes Page 176Lids Page 174Immuno Washers Page 76Pinchbar DesignImmobilizer is a trademark of Exiqon A/S, Vedbaek,Denmark. The product is produced under license fromExiqon A/S and covered by EP 08 20483 and foreignapplication and patents.Nunc Immobilizer F96 MicroWell PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case436006 Amino Clear 400 - - 5/30436014 Streptavidin Clear 400 - - 1/15436024 Nickel-Chelate Clear 400 - - 1/15436032 Glutathione Clear 400 - - 1/15Nunc F96 MicroWell PlatesPolystyrene. External dimensions 128 x 86 mmSee Immuno Assay section for details regarding Immobilizer platesCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case269620 Non-treated Clear 400 - - 5/60269787 Non-treated Clear 400 + - 1/50439454 * MaxiSorp Clear 400 - - 5/60442404 MaxiSorp Clear 400 - - 5/60475094 PolySorp Clear 400 - - 5/60* Certified146

www.nuncbrand.com - Always with updated informationF96 MicroWell Plates Polystyrene Black and White• White plates provide maximumreflection, minimum auto-fluorescenceand autoluminescence• Black plates provide minimalbackground and light scatterin fluorescence assays• Low crosstalk• MaxiSorp and PolySorp forsolid phase immuno assays• NunclonΔ for cell based assays• Working volume range:50 - 250 µl/wellPlatesLiterature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10, 11, 12, 15Tech Note Nos: 6, 12See Pages 195-196 for full reference list with titles and linksF1D1H3mmTotal height of plate 14.6 ± 0.3H2 Vertical distance from inside 3.6well bottom to resting planeH3 Inside depth of well 11.2Accessories:Racks Page 172Sealing Tapes Page 176Lids Page 174Immuno Washers Page 76H5H4D2H2H4 Vertical distance from external 2.1well bottom to resting planeH5 Height of flange 2.7D1 Well diameter, top 7.05D2 Well diameter, bottom 6.55 ± 0.1F1 Depth of flange 2.0Pinchbar DesignNunc F96 MicroWell PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case136101 * Cell Culture White 400 + + 1/50136102 * Cell Culture White 400 + + 10/160137101 * Cell Culture Black 400 + + 1/50137103 * Cell Culture Black 400 + + 10/160236105 Non-treated White 400 + + 1/50236107 ‡ Non-treated White 400 + + 10/160236108 Non-treated White 400 - - 10/180237105 Non-treated Black 400 + + 1/50237107 Non-treated Black 400 + + 10/160237108 Non-treated Black 400 - - 10/180436110 * MaxiSorp White 400 - - 10/80437111 * MaxiSorp Black 400 - - 10/80436111 PolySorp White 400 - - 10/80437112 PolySorp Black 400 - - 10/80* Certified‡ Not avaialbe in Americas147

Always with updated information - www.nuncbrand.comPlatesImmobilizer F96 MicroWell PlatesPolystyrene Black and White• Covalent attachment• No blocking or activationsteps required• High sensitivity and specificity• Highly specific binding• High signal to noise ratio• Amino surface for rapid couplingof peptides, proteins and DNA• Streptavidin surface for rapid couplingof biotinylated biomolecules such aspeptides, antibodies, oligonucleotidesor haptens• Glutathione surface for rapid coup l ingof GST-tagged fusion proteins• Nickel-Chelate surface for rapidcoup ling of 6x Histidine-taggedproteins• Certified reproducibility of binding:CV < 5% (well to well)• Working volume range:50 - 250 µl/well• Also available as clear (see Page 146)See Immuno Assay section for detailsregarding Immobilizer plates.Literature:Bulletin Nos. 1, 42Tech Note Nos. 43, 44, 45, 46, 47 48See Pages 195-196 for full reference list with titles and linksAccessories:Racks Page 172Sealing Tapes Page 176Lids Page 174Immuno Washers Page 76Pinchbar DesignNunc Immobilizer F96 MicroWell PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case436007 Amino White 400 - - 5/30436008 Amino Black 400 - - 5/30436015 Streptavidin White 400 - - 1/15436016 Streptavidin Black 400 - - 1/15436026 Nickel-Chelate White 400 - - 1/15436027 Nickel-Chelate Black 400 - - 1/15436033 Glutathione White 400 - - 1/15436034 Glutathione Black 400 - - 1/15Immobilizer is a trademark of Exiqon A/S, Vedbaek,Denmark. The product is produced under license fromExiqon A/S and covered by EP 08 20483 and foreignapplication and patents.148

www.nuncbrand.com - Always with updated informationC96 MicroWell Plates Polystyrene• C-shaped bottom for optimalwashing and liquid retrieval• FluoroNunc for fluorescencebased immuno assays with minimalauto-fluorescence• MaxiSorp and PolySorp forquantitative and qualitative solid phaseimmuno assays• Streptavidin suface for couplingof biotinylated biomolecules such aspeptides, antibodies, oligonucleotidesor haptens• NucleoLink surface for covalentheat-stable binding of DNA; optimizedfor DNA hybridization assays• Working volume range:50 - 250 µl/wellPlatesH5F1H4D1H3D2H2mmTotal height of plate 14.0 ± 0.3H2 Vertical distance from inside 3.1well bottom to resting planeH3 Inside depth of well 11.0H4 Vertical distance from external 2.1well bottom to resting planeH5 Height of flange 2.7D1 Well diameter, top 6.6D2 Well diameter, bottom 6.1F1 Depth of flange 1.2Literature:Bulletin Nos: 1, 4, 6, 7, 8, 9, 10, 11, 12, 15Tech Note Nos: 6, 12, 17See Pages 195-196 for full reference list with titles and linksNucleoLink Application Guide available at:www.nuncbrand.com/go/nucleolinkguideAccessories:Racks Page 172Sealing Tapes Page 176Lids Page 174Immuno Washers Page 76Pinchbar DesignNunc C96 MicroWell PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case236001 ‡ Streptavidin Clear 350 - - 1/15430341 MaxiSorp Clear 350 - - 5/60446612* MaxiSorp Clear 350 - - 5/60437958* MaxiSorp Clear 350 - - 5/60437796* MaxiSorp White 350 - - 5/60446140 PolySorp Clear 350 - - 5/60437869 ‡ PolySorp Clear ** 350 - - 5/60437842 PolySorp White 350 - - 5/60* Certified** FluoroNunc‡ Not available in AmericasNunc C96 MicroWell PlatesActivated heat-stable polymer. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case248150 ‡ NucleoLink Clear 350 - - 5/30‡ Not available in Americas149

Always with updated information - www.nuncbrand.comU96 MicroWell Plates Polystyrene ClearPlates• Clear plates with excellent opticalclarity• MaxiSorp and PolySorp for solidphase immuno assays• NunclonΔ surface for cell based assays• Sterile version for bacteriologicalapplications• Working volume range:50 - 250 µl/wellLiterature:Bulletin Nos: 1, 2, 4, 6, 7, 8, 9, 10, 11, 12, 15See Pages 195-196 for full reference list with titles and linksAccessories:Racks Page 172Sealing Tapes Page 176Lids Page 174Immuno Washers Page 76D1F1H4H3H2H6mmTotal height of plate 14.5 ± 0.1H2 Vertical distance from inside 4.2well bottom to resting planeH3 Inside depth of well 10.2H4Vertical distance from externalwell bottom to resting plane2.8H5 Height of flange on the short side 2.4H6 Height of flange on the long side 7.5D1 Well diameter, top 7.1D2 Well diameter, bottom Round bottomF1 Depth of flange 1.7High Flange DesignNunc U96 MicroWell PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case143761* Cell Culture Clear 300 + - 1/50163320* Cell Culture Clear 300 + + 1/50168136* Cell Culture Clear 300 + + 10/160260210 Non-treated Clear 300 + - 10/180262146 † Non-treated Clear 300 - - 135/135262162 Non-treated Clear 300 + - 1/50268152 Non-treated Clear 300 - - 10/180268200 Non-treated Clear 300 + + 10/160449824 MaxiSorp Clear 300 - - 5/60475434 PolySorp Clear 300 - - 5/60150*Certified† Only available in Americas

www.nuncbrand.com - Always with updated informationV96 MicroWell Plates Polystyrene Clear• Applications include: antibiotic screens,serological tests, storing and screeningcompounds and DNA libraries• The wells have raised rims and lids aresupplied with rings, which preventcrosscontamination• Well shape maximizes samplerecovery• Working volume range:50 - 250 µl/wellPlatesAccessories:Racks Page 172Sealing Tapes Page 176Lids Page 174Immuno Washers Page 76F1D1mmTotal height of plate 14.4 ± 0.1H3H2 Vertical distance from inside 4.6well bottom to resting planeH3 Inside depth of well 9.8H5H4H2H4 Vertical distance from external 2.8well bottom to resting planeH5 Height of flange 2.7D1 Well diameter, top 6.8D2 Well diameter, bottom V-shapedF1 Depth of flange 1.2Pinchbar DesignNunc V96 MicroWell PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case249662 Non-treated Clear 300 + - 1/50277143 ‡ Non-treated Clear 300 + + 1/50249935 Non-treated Clear 300 + + 10/80249940 Non-treated Clear 300 + - 10/80249952 Non-treated Clear 300 + + 10/160249570 Non-treated Clear 300 - - 10/180‡ Not available in Americas151

Always with updated information - www.nuncbrand.com96 Well Optical Bottom Plates Polymer BasePlates• NunclonΔ, Poly-D-Lysine or Collagen Isurfaces for cell culture• Non-treated plates are optimizedfor scintillation counting• White or black upper structure withpolymer bottom plates combine theoptical clarity of virgin crystallinepolystyrene with optimal surfacefor a wide range of HTS applications• Working volume range:50 - 200 µl/wellD1H2mmTotal height of plate 14.9Vertical distance from inside 2.55well bottom to resting planeLiterature:Bulletin Nos: 2, 15See Pages 195-196 for full reference list with titles and linksH5H3H2H4H3 Inside depth of well 9.8H4 Polystyrene film 0.25H5 Height of flange 6.2D1 Well diameter 6.5Accessories:Racks Page 172Sealing Tapes Page 176Lids Page 174High Flange DesignNunc 96 Well Optical Bottom PlatesPolystyrene/Polymer base. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Unit per pack /case165306 Cell Culture White 400 + + 10/30165305 Cell Culture Black 400 + + 10/30152040 Collagen I White 400 -* + 5/20152036 Collagen I Black 400 -* + 5/20152028 Poly-D-Lysine White 400 -* + 5/20152037 Poly-D-Lysine Black 400 -* + 5/20265302 Non-treated White 400 - - 10/30265301 Non-treated Black 400 - - 10/30* Produced in clean environment152

www.nuncbrand.com - Always with updated information96 Well Optical Bottom Plates Polystyrene/Coverglass• White or black upper stucture withcoverglass bottom for minimum lightscatter and low auto-fluorescence,ensuring accurate results due tohigher signal-to-noise ratios• Optimum clarity for viewingwell contents• CC² surface treatment closelymimics a biological surface similarto Poly-D-Lysine and is a superiorsurface for attachment and growthof fastidious cells• Non-treated plates are optimizedfor fluorescence• Working volume range:50 - 200 µl/wellPlatesH5D1H3H2mmTotal height of plate 14.9H2 Vertical distance from inside 2.5well bottom to resting planeH3 Inside depth of well 12.2H5 Height of flange 6.2D1 Well diameter 6.5Literature:Bulletin Nos: 2, 15See Pages 195-196 for full reference list with titles and linksAccessories:Racks Page 172Sealing Tapes Page 176Lids Page 174High Flange DesignNunc 96 Well Optical Bottom PlatesPolystyrene/Coverglass base. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Glass thickness * Sterile With lid Unit per pack /case164588 Cell Culture Black 400 1.5 + + 6/30164590 Cell Culture White 400 1.5 + + 6/30160376 CC² Black 400 1.5 + + 6/30265300 Non-treated Black 400 1.5 - - 5/30* No. 1.0 = 0.13-0.16 mm, No. 1.5 = 0.16-0.19 mm153

Always with updated information - www.nuncbrand.comU96 MicroWell Plates PolypropylenePlates• 0.5 ml volume in standardMicroWell format• Shared well technology increaseswell volume and wider cells improvemixing• Low binding polypropylene forhomo geneous assays and storage• Ideal as collection plate for NuncFilter Plates• White plates for fluorescenceand lumine scence applications• Black plates for fluorescenceapplications• Yellow, red, natural and blue platesfor storage applications• Resistant to most solvents usedin combinatorial chemistryH5F1H4D1H3H2mmTotal height of plate 14.5 ± 0.3H2 Vertical distance from inside 3.1well bottom to resting planeH3 Inside depth of well 11.2H4 Vertical distance from externalwell bottom to resting plane2.0• Working volume range:20 - 450 µl/wellAccessories:Filter Plates Page 159Racks Page 172Sealing Tapes Page 176Lids Page 174Well Caps Page 171Immuno Washers Page 76H5 Height of flange on the short side 2.4D1 Well diameter, top 8.5F1 Depth of flange 1.7High Flange DesignNunc U96 MicroWell PlatesPolypropylene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Unit per pack /case267245 Non-treated Natural 500 - - 10/120267334 Non-treated Natural 500 + - 10/120267342 Non-treated Black 500 - - 10/120267350 Non-treated White 500 - - 10/120267369 Non-treated Red 500 - - 10/120267385 Non-treated Blue 500 - - 10/120267407 Non-treated Yellow 500 - - 10/120154

www.nuncbrand.com - Always with updated informationV96 MicroWell Plates Polypropylene• V - shape well bottoms and PP materialprovide optimal recovery of reagents• Shared well technology increases wellvolume and wider cells improve mixingPlates• Low binding polypropylene forhomogeneous assays and storage• Total well volume of 0.45 ml• White plates for assays usingfluore scence and luminescence• Black plates for assays usingfluore scence• Yellow, red, natural and blue platesfor storage applications• Resistant to many solvents used instorage and combinatorial chemistry• Ideal as collector plates for NuncFilter Plates• Certified RNase/DNase-freeF1D1mmTotal height of plate 14.4 ± 0.1• Working volume range:10 - 400 µl/wellH5D2H3H4H2H2 Vertical distance from inside 3.4well bottom to resting planeH3 Inside depth of well 11.0H4 Vertical distance from external 2.5well bottom to resting planeH5 Height of flange on the short side 2.3H6 Height of flange on the long side 7.5D1 Well diameter, top 8.5D2 Well diameter, bottom V-bottomF1 Depth of flange 1.7Accessories:Filter Plates Page 159Racks Page 172Sealing Tapes Page 176Lids Page 174Well Caps Page 171Immuno Washers Page 76High Flange DesignNunc V96 MicroWell PlatesPolypropylene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Unit per pack /case249944 Non-treated Natural 450 - - 20/120249946 Non-treated Natural 450 + - 20/120249945 Non-treated Black 450 - - 20/120249949 Non-treated White 450 - - 20/120249943 Non-treated Red 450 - - 20/120249947 Non-treated Yellow 450 - - 20/120249950 Non-treated Blue 450 - - 20/120155

Always with updated information - www.nuncbrand.comV96 MicroWell Plates PolypropylenePlates• Resistant to most compound solventsused in combinatorial chemistry• Well shape maximizes sample recovery• Low binding polypropylene forhomogeneous assays and storage• Working volume range: 10 - 250 µl/well• Convenient package sizeAccessories:Filter Plates Page 159Racks Page 172Sealing Tapes Page 176Lids Page 174Well Caps Page 171Immuno Washers Page 76F1D1mmTotal height of plate 14.4 ± 0.1H3H2 Vertical distance from inside 4.6well bottom to resting planeH3 Inside depth of well 9.8H5H4H2H4 Vertical distance from external 2.8well bottom to resting planeH5 Height of flange 2.7D1 Well diameter, top 6.7D2 Well diameter, bottom V-shapedF1 Depth of flange 1.9Pinchbar DesignNunc V96 MicroWell PlatesPolypropylene. External dimensions 127 x 85 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Unit per pack /case442587 Non-treated Natural 300 - - 5/60156

www.nuncbrand.com - Always with updated informationU96 DeepWell Plates 1.3 ml & 2.0 ml Polypropylene1.3 ml 2.0 mlF1D1D1• Unique low design with shared welltechnology for increased well volume.Optimum storage capacity andimproved mixing• Round well shape reduces liquidretention• Well cap available for safe storage -minimal protrusion into each well• Ideal for sample collection, storage,combinatorial chemistry and libraryapplications• Resistant to most chemicals, solventsand alcohols used in combinatorialchemistry• Widely used for bacterial andyeast growth• Ideal as collection plates for NuncFilter Plates• Working volume range:Cat Nos. 260251 and 260252:50 - 1000 µl/wellPlatesDEEP WELL 1 MLH3H3Cat Nos. 278743 and 278752:50 - 1900 µl/wellLiterature:Bulletin Nos: 39, 40H2D2H4mmTotal height of plate 31.6H2 Vertical distance from inside 2.5well bottom to resting planeH3 Inside depth of well 29.1H4 Vertical distance from externalwell bottom to resting plane1.4D1 Well diameter, top 8.5D2 Well diameter, bottom 8.4F1 Depth of flange 2.5F1H5H2mmTotal height of plate 44.0Vertical distance from inside 2.5well bottom to resting planeH3 Inside depth of well 41.5H4 Vertical distance from external 1.5well bottom to resting planeH5 Height of flange 2.5D1 Well diameter, top 8.5D2 Well diameter, bottom 6.0F1 Depth of flange 1.5D2H2H4See Pages 195-196 for full reference list with titles and linksAccessories:Filter Plates Page 159Well Caps Page 171Nunc U96 DeepWell Plates 1.3 ml and 2.0 mlPolypropylene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., ml/ well Sterile With lid Unit per pack /case260251 Non-treated Natural 1.3 + - 5/50260252 Non-treated Natural 1.3 - - 5/50278743 Non-treated Natural 2.0 + - 1/60278752 Non-treated Natural 2.0 - - 5/60157

Always with updated information - www.nuncbrand.comPlates96 DeepWell Plates 1.0 ml PolystyreneNEW• Round bottom wells• Easy to use with automaticsample handling instruments• Alpha-numeric grid for quicksample identification• Ideal for sample collection,storage, combinational chemistrylibrary applications• Sealable with well capsand sealing tapeAccessories:Sealing Tapes Page 176NEWNunc 96 DeepWell PlatesPolystrene. External dimensions 128 x 86 x 41Cat. No. 278605 278606Configuration Round RoundPlate color Transparent TransparentTotal volume, ml 1.0 1.0Working volume, ml 0.9 0.9Sterile - +Units per pack/case 4/32 4/32158NEWNunc 96 Well Caps* for above 1.0 ml Nunc DeepWell PlatesExternal dimensions 119 x 77Cat. No. 278616MaterialEthylene-Vinyl AcetateSterile +Units per pack/case 1/25* Not suitable for DeepWell Plates Cat. Nos. 260251, 260252, 278752, 278743

www.nuncbrand.com - Always with updated information96 Filter Plates 1.3 mlFlow diagram for plasmid DNA purificationProcedure:Figure 1.Procedure onhow to useNunc FilterPlates for costeffective DNApurification.Grow E. Coli cells in a sterile 2 ml DeepWell Plate(Cat. No. 278743). (overnight)Lyse bacteria and neutralize lysate. (15 min.)Clarify plasmid DNA with Fritted Filter Plate(Cat. No. 278011). (1 min.)Bind plasmid DNA to Glass Fiber Filter Plate(Cat. No. 278010). (3 min.)Wash and dry membrane. (8 min.)Elute pure plasmid DNA with TE into 1 ml receptionDeepWell Plate (Cat. No. 260252). (12 min.)• For efficient purification of highquality plasmid DNA or PCR products• Designed specifically for highthroughput sample processing• Conforms to standard 96 well footprint• Made of polypropylene offeringchemical resistance and mechanicalstrength• Suitable for vacuum filtration, centrifugationor positive pressure techniques• Polyethylene terapthalate (PET) frit infritted plate has 20 µm pore size• Glass fiber plate has PET frit and 2 layersof glass fiber membrane forbinding of DNA• Shared wall configuration givesreduced plate height, wider wellsfor faster processing, higher yieldsand less clogging• Purified DNA is suitable fordownstream applications such assequen cing and restriction digestionLiterature:Bulletin Nos: 39, 40See Pages 195-196 for full reference list with titles and linksAccessories:96 DeepWell Plates Page 157-158Sealing Tapes Page 176U96 MicroWell Plates 500 μl Page 154V96 MicroWell Plates 450 μl Page 155PlatesNunc 1.3 ml Filter PlatesCat. No. 278010 278011 278012DescriptionGlass Fiber 96 Well FilterFritted 96 DeepWell PlateUnfritted 96 DeepWell Plate(Binding) PlateTotal volume, ml 1.3 1.3 1.3Recommended1.0 1.0 1.0working volume:Frit Size, µm 20* 20 -Material Polypropylene Polypropylene PolypropyleneSterile - - -Units per pack/case 5/50 5/50 5/50* Has 2 layers of glass fiber membrane on top of the PET frit159

Always with updated information - www.nuncbrand.comImmobilizer 384 Well Plates PolystyrenePlatesD1F1mmTotal height of plate 14.4 ± 0.25H2 Vertical distance from inside 2.6well bottom to resting planeH3 Inside depth of well 11.7Literature:Bulletin Nos: 41, 42, 43 , 44, 45, 46, 47, 56See Pages 195-196 for full reference list with titles and linksH2D2H3H4H5H4 Vertical distance from external 1.7well bottom to resting planeH5 Height of flange 2.7D1 Well diameter, top 3.7D2 Well diameter, bottom 2.7F1 Depth of flange 1.9Accessories:Racks Page 172Sealing Tapes Page 176Lids Page 174See Immuno Assay section for detailsregarding Immobilizer plates.Pinchbar DesignNunc Immobilizer 384 Well PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case436009 Amino Clear 120 - - 5/30436011 Amino White 120 - - 5/30436012 Amino Black 120 - - 5/30436017 Streptavidin Clear 120 - - 1/15436018 Streptavidin White 120 - - 1/15436019 Streptavidin Black 120 - - 1/15436028 ‡ Nickel-Chelate Clear 120 - - 1/15436029 ‡ Nickel-Chelate White 120 - - 1/15436031 ‡ Nickel-Chelate Black 120 - - 1/15436036 ‡ Glutathione Clear 120 - - 1/15436037 ‡ Glutathione White 120 - - 1/15436038 ‡ Glutathione Black 120 - - 1/15‡ Not available in Americas160

www.nuncbrand.com - Always with updated information• Amino surface for rapid couplingof peptides, proteins and DNA• Streptavidin surface for rapidcoupling of biotinylatedbio molecules such as peptides,antibodies, oligonucleotides orhaptens• Nickel-Chelate surface for rapidcoupling of 6x Histidine-taggedproteins• Glutathione surface for rapidcoupling of GST-tagged fusionproteins• Cell culture surface available• Non-treated surface for storage,assays and screening• MaxiSorp and PolySorpfor ELISA• Clear plates for colorimetric assaysand sample storage• White plates for luminescenceand fluororescence assays• Black plates for fluorescence assays• White and black platesminimize crosstalk• Rounded square wellseliminate wicking (capillary action)• Flat bottom for optical reading• Optimized for scintillation counting• Working volume range:10 - 100 µl/wellPlatesPinchbar DesignNunc 384 Well PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case164688* Cell Culture Clear 120 + + 10/30164555* Cell Culture Clear 120 + - 25/100164610* Cell Culture White 120 + + 10/30165195* Cell Culture White 120 + - 25/100164564* Cell Culture Black 120 + + 10/30164571* Cell Culture Black 120 + - 25/100242757 Non-treated Clear 120 + + 10/30242765 Non-treated Clear 120 + + 1/30262160 Non-treated Clear 120 - - 25/100265196 ‡ Non-treated Clear 120 - - 10/30242850** ‡ Non-treated Clear 120 + + 10/30265202 Non-treated Clear 120 + + 25/100265203 Non-treated Clear 120 + - 25/100262360 Non-treated White 120 - - 25/100264556 ‡ Non-treated Black 120 - - 10/30262260 Non-treated Black 120 - - 25/100460440 ‡ PolySorp Clear 120 - - 10/30460435 PolySorp Black 120 - - 10/30464718* MaxiSorp Clear 120 - - 10/30460372* MaxiSorp White 120 - - 10/30460518* MaxiSorp Black 120 - - 10/30* Certified** Barcoded on the left short side with Code 39‡ Not available in Americas161

Always with updated information - www.nuncbrand.comPlates384 ShallowWell Standard Height Base PolystyreneA4.5 mm12.1 mm103.5 mmNEW• Conical flat bottom for cell cultureor assays• 25 µl total volume saves reagents• Standard height providesoptimal handling, barcoding• Clear, black or white for colorimetric,fluorescent and luminescent assays• Working volume range: 2 - 20 µl/well9 mm4.5 mm67.5 mmØ 3 mm5.21 mm81.1 mm1.14 mm14.35 mm2.16 mm12.1 mm103.5 mm4.5 mm9 mmA67.5 mm4.5 mm81.1 mmNEW85.5 mmNunc 384 ShallowWell, Standard Height PlatesPolystyrene. Maximum volume 25μl, working volume 2 - 20 μlCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case164701 Cell Culture Clear 25 + + 25/100164702 Cell Culture Black 25 + + 25/100164703 Cell Culture White 25 + + 25/100264704 Non-treated Clear 25 - - 25/100264705 Non-treated Black 25 - - 25/100264706 Non-treated White 25 - - 25/100162

www.nuncbrand.com - Always with updated information384 ShallowWell Plates Polystyrene• Low profile design for maximumstacking capacity• Plate height optimized for topand bottom reading• Available with unique, low profilelid (LowBot lid)• Conical wells with flat bottom• Cylindrical well top for minimizingevaporation• Highly rigid plate, for secure robothandling• Packed in antistatic bags• Elevated rims allow heat sealingof wells• NunclonΔ surface for cell culture• Working volume range: 2 - 20 µl/wellPlatesH3H2D1H4F1H6H5mmTotal height of plate 7.6 ± 0.25H2 Vertical distance from inside 2.4well bottom to resting planeH3 Inside depth of well 5.2 ± 0.05H4 Vertical distance from externalwell bottom to resting plane1.65Accessories:Racks Page 172Sealing Tapes Page 176Lids Page 174D2H5 Height of flange 2.54H6 Height of rim 0.2D1 Well diameter, top 3.0D2 Well diameter, bottom 1.5 ± 0.05F1 Depth of flange 2.0 ± 0.05Nunc 384 ShallowWell PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case164310* ‡ Cell Culture Clear 25 + + 25/100164400* ‡ Cell Culture Black 25 + + 25/100264340 ‡ Non-treated Clear 25 - - 25/100264360 ‡ Non-treated Clear 25 + + 25/100264430 ‡ Non-treated Black 25 - - 25/100264460 ‡ Non-treated Black 25 + + 25/100*Certified‡ Not available in Americas163

Always with updated information - www.nuncbrand.com384 Well Optical Bottom Plates Polystrene/Polymer BasePlates• White or black upper structureswith optically clear bottoms• Can be read from bottomand top, or both• Optical film made of polystyrene• Rounded square wellseliminate wicking (capillary action)• Optimized for scintillation counting• Cell Culture, Poly-D-Lysine or Collagen Isurfaces for cell culture• Produced in clean environment• Working volume range: 10 - 100 µl/well• For research use onlyF1H3D1H7mmTotal height of plate 14.4 ± 0.25H2 Vertical distance from inside 2.7well bottom to resting planeH3 Inside depth of well 11.6Literature:Bulletin No: 15See Pages 195-196 for full reference list with titles and linksH5D2H2H4H4 Vertical distance from external 2.5well bottom to resting planeH5 Height of flange 2.7H7 Bottom thickness 0.3D1 Well diameter, top 3.7 x 3.7D2 Well diameter, bottom 2.9 x 2.9F1 Depth of flange 1.8Accessories:Sealing Tapes Page 176Lids Page 174Pinchbar DesignNunc 384 Well Optical Bottom PlatesPolystyrene/Polymer base. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case142762* Cell Culture White 120 + + 10/30142761* Cell Culture Black 120 + + 10/30164730* Cell Culture Black 120 + - 10/30152041 Collagen I Black 120 -** + 5/20152029 Poly-D-Lysine Black 120 -** + 5/20242763 Non-treated White 120 - - 10/30242764 Non-treated Black 120 - - 10/30164* Certified** Produced in clean environment

www.nuncbrand.com - Always with updated information384 Well Optical Bottom Plates Polystyrene/Coverglass BaseNEW• Black upper structure with coverglassfor minimum light scatter and lowauto-fluorescence, ensuring accurateresults due to high signal to noise ratio• Optimum clarity for viewingwell contents• Flat bottom well for plate reader access• Rounded square wells eliminatewicking (capillary action)• Footprint compatible with standardequipment and automated systems• Non-treated plates are optimizedfor fluorescence• Working volume range: 10-100 µl/wellPlatesAccessories:Lids Page 174Sealing Tapes Page 176Pinchbar DesignNEWNunc 384 Well Optical Bottom PlatesPolystyrene/Coverglass base. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Glass thickness * Sterile With lid Units per pack/case164586 Cell Culture Black 120 1.5 + + 6/30240074 Non-treated Black 120 1.5 - - 5/30* No. 1.5 =0.16-0.19 mm165

Always with updated information - www.nuncbrand.com384 Well Plates PolypropylenePlates• Different colors for storage applications• Low-binding surface• White plates for luminescent andfluorescent homogeneousapplications (SPA)• Black plates for fluorescenthomogeneous applications• Round bottom wells for optimalsample recovery• Rounded square wells minimizewicking (capillary action)• Resistant to many solvents used instorage and in combinatorialchemistry• Certified RNase/DNase-free• Working volume range: 10 - 100 µl/wellD1H3F1mmTotal height of plate 14.4 ± 0.25H2 Vertical distance from inside well 2.7bottom to resting planeH3 Inside depth of well 11.7H4 Vertical distance from external well 1.8bottom to resting planeLow Cross TalkHigher density and therefore- low cross talk- highly reflective surface- with low capillary actioncompared to white polystyreneH2D2H4H5 Height of flange 2.7D1 Internal well dimensions, top 3.7D2 Internal well dimensions, bottom 3.0F1 Depth of flange 1.8Accessories:Racks Page 172Sealing Tapes Page 176Lids Page 174Pinchbar DesignNunc 384 Well PlatesPolypropylene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case264573 Non-treated Natural 120 - - 20/120264574 Non-treated Natural 120 + - 20/120264575 Non-treated White 120 - - 20/120264576 Non-treated Black 120 - - 20/120240182 Non-treated Black 120 + - 20/120264579 Non-treated Blue 120 - - 20/120264675 Non-treated* White 120 - - 20/120* Low cross talk166

www.nuncbrand.com - Always with updated information384 ShallowWell Standard Height Plates PolypropyleneNEW• Small volume applications• Automation compatible, ANSI standard• Low-binding and chemicallyresistant surface• Natural plates for storageor colorimetric assays• White plates for luminescent assays,including SPA• Black plates for fluorescent assays• Round bottom wells for optimalsample recovery• Rounded square wells minimizewicking (capillary action)• Working volume range: 2-35 µl/wellPlatesAccessories:Sealing Tapes Page 176Lids Page 17412.1 mm103.5 mm4.5 mm9 mmA67.5 mm4.5 mm81.1 mm85.5 mmNunc 384 ShallowWell PlatesPolypropylene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol, µl/well Sterile With lid Units per pack/case267459 Non-treated Natural 58 - - 25/100267460 Non-treated Natural 58 + - 25/100267461 Non-treated Black 58 - - 25/100267462 Non-treated White 58 - - 25/100167

Always with updated information - www.nuncbrand.com384 DeepWell Plates PolypropylenePlates• Excellent for storing DMSO andother strong chemicals and solvents• Well shape maximizes sample retrieval• Compatible with robotics andauto mation• Rounded square wells minimizewicking (capillary action)• Barcoding available by arrangement• Sealable with chemical-resistantwell cap mats• Working volume range: 5 - 240 µl/wellAccessories:Racks Page 172Lids Page 174Well Caps Page 171Sealing Tapes Page 176D2mmH1 Total height of plate 22 ± 0.5H2Vertical distance from inside wellbottom to resting plane2.5H3H2 H4D1H5H1 H3 Inside depth of well 20.0H4 Vertical distance from external well 2.0bottom to resting planeH5 Height of flange 2.5D1 Well diameter, top 3.9D2 Well diameter, bottom 3.4F1 Depth of flange 1.8Nunc 384 DeepWell PlatesPolypropylene. External dimensions 128 x 86 mmCat. No. Surface Color Total vol., µl/well Sterile With lid Units per pack/case269390 Non-treated Natural 252 - - 5/60168

www.nuncbrand.com - Always with updated information1536 Well Plates Polystyrene• Available in black, white and clear• Working volume range: 1 - 10 µl/well• Non-treated or MaxiSorp treated• Low plate height optimized forstacking efficiency• Working volume range: 1 - 10 µl/wellPlatesAccessories:Racks Page 172Sealing Tapes Page 176Lids Page 174D1F1mmTotal height of plate 7.4H4H2D2H3H5H2 Vertical distance from inside 2.4well bottom to resting planeH3 Inside depth of well 5.0H4 Vertical distance from external 1.5well bottom to resting planeH5 Height of flange 2.5D1 Well diameter, top 1.7D2 Well diameter, bottom 1.5F1 Depth of flange 1.8Nunc 1536 Well PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total volume, µl/well Sterile With lid Units per pack/case453603 MaxiSorp* Black 13.4 - - 30/90253614 Non-treated Clear 13.4 - - 30/90253601 Non-treated Black 13.4 - - 30/90253607 Non-treated White 13.4 - - 30/90* Certified169

Always with updated information - www.nuncbrand.comPlates1536 Well High Base Plates PolystyreneNEW• Optimal robotic handling• Preferred skirt design• Maximal barcode placement area• Non-treated or cell culture surface• Fluorescent, luminescent andcolorimetric assays• ANSI (SBS) standard footprint l x w• Low volume saves reagents• Working volume range: 2-10 µl/wellAccessories:Lids Page 174Sealing Tapes Page 176NEWNunc 1536 Well High Base PlatesPolystyrene. External dimensions 128 x 86 mmCat. No. Surface Color Total volume, µl/well Sterile With lid Units per pack/case164707 Cell Culture Clear 13.4 + + 25/100164708 Cell Culture Black 13.4 + + 25/100164709 Cell Culture White 13.4 + + 25/100264710 Non-treated Clear 13.4 - - 25/100264711 Non-treated Black 13.4 - - 25/100264712 Non-treated White 13.4 - - 25/100170

www.nuncbrand.com - Always with updated informationWell Cap Mats• Protect well contents during shortor long-term storage of chemicalsor compounds• Chemically resistant, pierceablethermo plastic elastomer well capsare excellent for compound storageto -80°C• Silicone pre-slit well caps permita pipette tip or probe to enter thewell without damage to the cap• Ethylene-vinyl acetate cap matseffectively seal polystyreneDeepWell plates• Cat. No. 276011 is pierceableby pipette tips• 96 well caps for sealing all Nunc PPplates with shared wall technologyTeflon is a registered trademark of DuPontPlate AccessoriesNunc 96 Cap MatsCat. No. 96 PP* 96 DW Features Material Pierce** Color Sterile Packed276000 + + Resists DMSO, ethanolThermo-plastic Yes Natural + 1/50and methanolelastomer276002 + + Resists DMSO, ethanoland methanol276003 ‡ + + Resists DMSO, ethanoland methanol276004 ‡ + + Resists DMSO, ethanoland methanol276005 + + Resists DMSO, ethanoland methanol276011 + + Pre-slit; solvent resistant,but avoid 100% DMSO* Except Cat. No. 442587, 96 Polypropylene Plates** Hypodermic needles*** Also by pipette tips‡ Not available in AmericasThermo-plasticelastomerYes Natural - 5/50Thermo-plastic Yes Yellow - 5/50elastomerThermo-plastic Yes Red - 5/50elastomerThermo-plastic Yes Blue - 5/50elastomerSilicone Yes, *** Natural - 10/50repeatedly171

Always with updated information - www.nuncbrand.comMicroplate Storage RacksPlate AccessoriesPlastic Plate Storage Racks in Colors• Transport or store up to 50 standardplates or 25 DeepWell plates per rack• For chest and upright mechanicalfreezers or incubators• Sturdy polypropylene and metalconstruction is lightweight, durable• Available in four colors to organize yoursamples• Locking bar keeps plates secure• Handle allows convenient transport• Freezer compatible down to -80°C• Not for use in liquid/vapor nitrogenFor Standard PlatesCat. No. 344260 † 344261 † 344262 † 344263 † 344280 † 344281 † 344282 † 344283 †Capacity (plates) 10 10 10 10 50 50 50 50Color Green Purple Red Yellow Green Purple Red YellowConfiguration 10 Tall 10 Tall 10 Tall 10 Tall 10 x 5 10 x 5 10 x 5 10 x 5Size H x W x D, cm: 344260 - 344261 - 344262 - 344263 = 23 x 10 x 14344280 - 344281 - 344282 - 344283 = 23 x 51 x 14Height per shelf, cm 2 2 2 2 2 2 2 2Units per case 1 1 1 1 1 1 1 1† Only available in Americas172For DeepWell PlatesCat. No. 344380 † 344381 † 344382 † 344383 †Capacity (plates) 25 25 25 25Color Green Purple Red YellowConfiguration 5 x 5 5 x 5 5 x 5 5 x 5Size H x W x D, cm: 344380 - 344381 - 344382 - 344383 = 25 x 51 x 14Height per shelf, cm 5 5 5 5Units per case 1 1 1 1† Only available in Americas

www.nuncbrand.com - Always with updated informationAluminum Racks in Colors• Adjustable shelves to fit any plate ortube rack• Color coded top and sides for easyidentification• Locking bar keeps plates secure• For chest and upright mechanical freezersor incubators• Autoclavable• Accommodates:--18 Low Profile--10 Standard--6 DeepWell 1 ml--5 DeepWell 2 mlPlatesAluminum Plate Storage RacksCat. No. 367001 367002Color Blue NaturalConfiguration 5 - 18 Tall 5 - 18 TallSize, H x W x D, cm 23.5 x 9.5 x 13.5Height per shelf, cm Adjustable AdjustableUnits per case 1/1 1/1Stainless Steel Plate Storage Racks• Durable stainless steel construction• Retainer clip on each compartmentholds plates securely in place• Sturdy handles on top and sides foreasy transport• Label holder for easy identification• Two convenient horizontal sizes to fityour freezer or refrigeratorCat. No. 5039-0048 5039-0072Capacity (plates)80 Low Profile48 Standard16 DeepWell120 Low Profile72 Standard24 DeepWellColor Natural NaturalConfiguration 4 Tall x 4 Wide 4 Tall x 6 WideSize, H x W x D, cm 21.6 x 48.3 x 14 21.6 x 57.9 x 14Height per shelf, cm 5.3 5.3Units per case 1/1 1/1173

Always with updated information - www.nuncbrand.comOverview of Nunc LidsPlate Accessories• Clear, strong polystyrene• Nunc version lids have notchesfor quick orientation• Universal version lids can beused with any plates• Reduces water loss dueto lower evaporation• 96-well lids feature individualcondensation rings• LowBot lids feature sidecut-outs that facilitate barcodereading and automated handlingof shallow well platesCat. No.Height withoutstacking holders, mm Cut-off corners Condensation rings Evaporation Barrier* Height Color Sterile PackedNunc Standard Lids, Nunc Version for 96 MicroWell PlatesWith condensation rings and evaporation rim. Polystyrene. External dimensions 127 x 85 mm263339 7.9 Long side + + Standard Clear - 5/100264122 7.9 Long side + + Standard Clear + 1/50264623 7.9 Long side + + Standard Clear + 20/60Nunc Standard Lids for 96 Optical Bottom PlatesWith condensation rings and evaporation rim. Polystyrene. External dimensions 127 x 85 mm255983 7.8 Right short side + + Standard Clear - 20/60Nunc Standard Lids, Nunc Version for 384 Well PlatesWith evaporation rim. Polystyrene. External dimensions 127 x 85 mm264611 7.9 Long side - + Standard Clear + 1/60264612 7.9 Long side - + Standard Clear - 20/180264616 7.9 Long side - + Standard Clear + 20/180Nunc White Lid, Nunc version for 384 and 1536 Well Plates and OmniTraysPolystyrene. External dimensions 127 x 85 mm448143 7.9 Long side - - Standard White - 20/120Nunc Universal Lid for 384 and 1536 Well PlatesWith side cut-out and evaporation rim. Polystyrene. External dimensions 128 x 86 mm250002 8.34 - - - Standard Clear + 20/60250003 8.34 - - - Standard Clear - 20/60250005 8.34 - - - Standard Clear - 20/180Nunc LowBot Lids, Universal Version for 384 and 1536 Well PlatesWith side cut-out and evaporation rim. Polystyrene. External dimensions 128 x 86 mm253623 4.6 - - + Low profile Clear - 25/100253624 4.6 - - + Low profile Clear + 25/100* Evaporation barrier is a rim around the inside edge of the lid174

www.nuncbrand.com - Always with updated informationNunc Lid GuidePlate AccessoriesCat. No.Type 1 Plates96 Solid MicroWell*PS, PPType 2 Plates96 Optical BottomNunc Standard Lids, Nunc Version for 96 MicroWell PlatesType 3 Plates384 Standard Height,384 ShallowWell PSStandard Height,1536 High BaseType 4 Plates384 ShallowWellLow Profile, 1536Low Profile263339 + - - - -264122 + - - - -264622 + - - - -264623 + - - - -264626 + - - - -Nunc Standard Lids for 96 Optical Bottom Plates255983 - + - - -Nunc Standard Lids, Nunc Version for 384 and 1536 Well Plates264611 + - + - -264612 + - + - -264616 + - + - -Nunc White Lid, Nunc Version for 384 and 1536 Well Plates and Omnitrays448143 + - + - -Nunc Standard Lids, Universal Version for Standard Height 384 and 1536 Well Plates250002 + + + - +250003 + + + - +250005 + + + - +Nunc LowBot Lids, Universal Version for 384 and 1536 Well Plates253623 + + + + +253624 + + + + +* Not 1 ml DeepWell PP (278605 and 278606)Type 5 Plates96 DeepWell(278605 and 278606),384 ShallowWell PP175

Always with updated information - www.nuncbrand.comSealing TapesPlate Accessories236707236710236702236701• Effectively seal all microplate formats• For storage, PCR, microscopy, culture,protection• Protective backing• Pre-cut, plate-sized sheets• Pierceable tapes are available176NEWNEWNEWNEWNEWNEWNEWNEWSealing TapesCat. No. Description Material Adhesive Color Sterile Pierce276014 General use, PCR, storage,photo-sensitive samplesCertified RNase/DNase-freeTemperatureRange, °CPackedAluminium Silicone Silver - + + -80 to +120 100/100232698 PCR and storage in 96 well plates Aluminium Acrylate Silver - + + -80 to +150 100/100232699 PCR and storage in 384 well plates Aluminium Acrylate Silver - + + -80 to +150 100/100236370 † General purpose seal and sample Polyester Silicone Clear - - - -70 to +100 100/100protection236269 ‡ Slightly stiffer than polyolefin Polyester Acrylic Clear - - - -20 to +100 200/800tape, general purpose235305 Opaque, white seal, luminescent Polyester Acrylate White + - - -70 to +100 100/100assays236366 Cell culture, sterile applications Polyester Acrylic Clear + - - -70 to +100 200/800236707 Convenient roll dispenser,Polyester Acrylic Clear - - - -20 to +100 1000/1000general use tape235306 Repeat piercing, non sticky,Polyethylene Silicone Clear - + - -70 to +100 100/100low fluorescence, storage232701 Microscopy, good optics, low Polyolefin Acrylate Clear - - - -70 to +100 100/100auto-fluorescence, DMSOcompatible232702 PCR, fluorescence assays,Polyolefin Acrylate Clear - - + -70 to +100 100/100microscopy235307 Best optics, microscopy,Polyolefin Silicone Clear - - - -70 to +100 100/100lowest fluorescence, Q-PCR236702 Black alpha numeric guide on Polypropylene Acrylate Clear - - - N/A 100/10096 well plate bottom label249720 Breathable Rayon Acrylate White - + - -20 to +80 50/50241205 Breathable, cell culture Rayon Acrylate White + + - -20 to +80 25/50236703 Apply to plate top or bottom,protect light sensitive samples236701 Repeat piercing, pre-cut “X”self closes, short term storage† Only available in Americas‡ Not available in AmericasVinyl Acrylate Black - - - -40 to +80 50/50Vinyl Acrylate Clear - + - -40 to +80 100/100

www.nuncbrand.com - Always with updated information• Clear, pierceable polyester strips• -20°C to 100°CPlate AccessoriesTape 12 and Tape 48Cat. No. Description Color Sterile Units per pack/Case232689 Seal one row of a96 MicroWell Plate232700 Seal 4 rows of a96 MicroWell PlateClear - 100/400Clear - 100/400ApplicatorCat. No. Description Color Sterile Units per pack/Case250050 Rectangular, hand held, promotes Gold - 2/2consistent seal177

Always with updated information - www.nuncbrand.com

www.nuncbrand.com - Always with updated informationCustom Services IntroductionWe understand your needs- and meet your demandsOur highly experienced specialtyteams are ready to bring you thevery best solutions for your uniqueapplications.Our commitment to ourcustomers includes:• Innovative solutions• Flexibility• R & D support• Customization• Functional surfaces• Coating• Design• Batch reservation• Lot-to-lot consistencyDeveloping tomorrow’s productsProduct development has alwaysbeen a cooperative effort to createinnovative products to suit individualrequirements. As an extension of this,we have established teams using ourexpert staff. They will support youridea from the beginning throughimplementation.ContentsNunc Custom Coatingof MicroWell Plates.................................180Customized Products................................181Custom Molding.........................................181Barcoded Products....................................182Custom Services179

Always with updated information - www.nuncbrand.comNunc Custom Coating of MicroWell PlatesCustom ServicesNunc Brand researchknowledge and productionskills can decrease yourtime-to-marketManufacturers of diagnostic kits, aswell as screening departments withinpharmaceutical companies, can quicklybenefit from our new plate-coatingservice.Access Nunc Brand’s comprehensiveknowledge and experience in producingMicroWell plates when you need customspecific-protocol plate coating. Assistingyou at every step is a team with deepknowledge and expertise in immunodiagnostics and surface modification.The team’s goal is optimal binding ofyour target molecule to a MicroWellplate. Nunc Brand is committed tooffering reliable, cost-effective customassay development and productionoptimization.Basically stated, we help develop, thenapply your custom coating on ourstandard microwell plates and ImmunoModules, using our reliable, provenmicroplate processing equipment. Thismix of new and existing processes/equipment can markedly decreaseexpense and increase availability of yourunique application.To learn more about our new service,contact us at:diagnostics.nunc@thermofisher.comThe combination of Nunc Immuno Modulescoated by Nunc and packaging vials by Nalgeneprovide a well-documented product.Optimize Your Opportunities - Outsource YourPlate-Coating OperationsOptimize your production capacity• Increase your production flexibility• Access secondary productionproduction facilities• Avoid backorders• Use an independent coating facilityOptimize resources• Reduce your investments• Focus on value-added projects• Allocate assets effectivelyOptimize time to market• Use Nunc knowledge in--Surface treatment--Surface capabilities--Production skills• Improve your output• Stay ahead of your competitorsQuality ControlOur highly skilled QC and QAtechnicians are at your disposal toprovide the quality documentationnecessary to fulfil your requirements.Our controlled environment facilitiesassures a high consistency throughoutthe entire production run.We are able to offer• Full Quality Control and Certificationof conformity according to yourspecifications• Production using calibrated equipmentaccording to controlled SOPs• All product documentation controlledby our QA Department180

www.nuncbrand.com - Always with updated informationCustomized ProductsAll Nunc Brand Productscan be customizedSelect your options:• Special color coding• Special barcoding• Specialized surfaces on all modules• Also available: lot reservations, lotapproval, and samples for testing.Please contactinfo.nunc@thermofisher.com directly.Custom ServicesR - COOH Poly-His - RLIPIDSBiotin - RGLYC ANSGLYCOL IPIDSPolySorpGLYCOPR O TEINSLIPOPROTEINSMaxiSorpMultiSorpMediSorpPROTEINSR - NH 2+GAH +EDCpH 9.6 pH 7.5CovaLinkR - SHAmino(Electrophile)PSStreptavidinNi-chelateGlutathioneGST - RCustom MoldingIf you require a custom designed productfor your automated immuno analyzer,microfluidic based system, microarrays,or other applications please contact ourspecialists at:info. nunc@thermofisher.com.You can also make contact to any of ourwell-known specialists and dealers ofNunc Brand Products.Close Collaboration Yields Quality Custom Products• Initial meeting between you and NuncBrand product specialists evaluate anddefine the technolgy. E.g. the need forcertified or special surfaces or the usefor other Nunc Brand capabilities.• A mutual Secrecy Agreement maybe needed• A technical meeting covering theresearch or the molding aspects isarranged. At this stage necessary QCtest parameters are defined.• Based upon this information aquotation covering all costs inthe project and product pricing isgenerated. The quotation will includea definition of ownership and otherparameters, as well as a time schedule.• After agreeing on the actualdesign (perhaps including a lasermanufactured Beta-model) the moldand the production units will bemanufactured according to the timeschedule.• During this period the OEM Managerand the Technical Department willissue a monthly report and othercommunication as necessary.• Test and validation will be made andthe final product shipped followingNunc QC specialist and customerapproval.181

Always with updated information - www.nuncbrand.comBarcoded ProductsCustom Services182Barcodes play an essential role in tracking samples. Theyprovide a tool for reviewing the large quantities of data. As aconsequence, Nunc Brand Products offer barcoded products– either as part of our standard assortment (F96 new standarddesign and barcoded flasks with existing catalog numbers) oras customized products, e.g. barcoded CryoTubes, barcodedMicroWell plates and other Nunc Brand Products.We offer the complete range of barcoded products plus 2scanners to read barcodes.Why use barcodes?A barcode provides the safest way to keep track of your sample.The code is extremely durable and will help reduce humanerrors. Barcoded products are suitable for automated or manualoperations. Some barcoded products provide a trouble-freehuman readable code, which can be manually entered when ascanner is not available.BarcodesA barcode is a “machine-readable code” which can be usedwhen many samples are to be processed, analyzed or stored bymeans of automated equipment.The advantages of using barcodes are:• Improved accuracy (see chart below)• Increased efficiency• Higher safety levels• Reduced costsTime Requiredto key 20 char.Accuracy 1 error / 300charactersManual Entry Barcode Scanning10 seconds 4 seconds1 error / 10 millioncharactersAvailable codes:Code 39Code 39 is an alphanumerical code, meaning that it hasboth numbers and letters. It can have variable lengths and isself-checking. Every Code 39 character has five bars and fourspaces. Code 39 is self-checking.Interleaved 2 of 5Int 2 of 5 is numerical code (no letters) and is also self-checkingto improve the data security. Each Interleaved 2 of 5 charactersencodes two digits (one in the bars and one in the spaces).Each character has five bars or five spaces (two broad and threenarrow). It is recommended to have a check digit if possible.Code 128Code 128 is also alphanumerical, but has a higher density thanCode 39. Code 128 character comprises three bars and threespaces. Code 128 encodes the full ASCII 128 character set, andhas 107 different printed characters. Each character can haveone of three different meanings, depending on which subsetof characters being used initially. Subset A, Subset B and SubsetC are the three different subsets.Prefix/suffixPrefixes/suffixes are a fixed group of characters that can appearon a barcode. Prefixes are available before the variable numbersor suffix after the variable numbers. e.g. “NU” in a 10 digitnumeric code counting up from start number NU00000001.The prefix might help in identifying different companies orseries of data.ANSI-Grade scaleWith the three mentioned codes, we follow the standardizedANSI X3.182- 1990 guideline (Barcode Print Quality-Guideline) (ANSI = American National Standard Institute).Barcoded platesWhen you require barcoded MicroWell plates, we can offertwo different solutions: Nunc standard products and Nunccustom barcode plates.On standard barcoded Nunc plates, a seven-digit Code 39alphanumeric label is applied to the short left side of the plate.On customized barcoded plates, we offer Code 128, Code 39and Interleaved 2 of 5.You have the flexibility to choose:• The number of digits• Code type• Numerical or alphanumerical• Label placement (one, two, three or all four sides)• Prefix or SuffixFor further information and to download the barcoded platerequest, please look at the barcode section atwww.nuncbrand.com, or contact Nunc Brand directly atbarcode.nunc@thermofisher.com.Barcoded CryoTubesBarcodes can be placed on CryoTubes in two different ways:Pad-print/Ink JetThis is done by printing directly on the CryoTube. First a whitebackground is pad-printed, then the ink jet technique is used toprint the black codes on the white background. These codes canwithstand the temperature fluctuations that a CryoTube wouldbe exposed to in freezing liquid nitrogen and the followingdefrosting.LabelThis solution is unique because the codes can withstandmost chemicals, e.g. toluene, acetone and DMSO. Like thepad-printed version, these codes can withstand temperaturefluctuations, freezing in liquid nitrogen and the followingdefrosting.

www.nuncbrand.com - Always with updated informationBarcoded CryoTubes CapabilitiesMethod Ink-jetted* Label Label Label Label Label LabelDescription and Packedbase productcatalog numberInterleaved2 of 5Max 9 digits(**)Code 39Max. 3digitsSmall labelCode 39Max. 6digitsLarge labelCode 128Max. 7digitsSmall labelCode 128Max. 13digitsLarge labelInterleaved2 of 5Max 9 digitsSmall labelInt. round b. 3.6 ml 50/500/2000 Yes Yes Yes Yes Yes Yes Yes(366524)Int. round b. 4.5 ml 50/400/1600 Yes Yes Yes Yes Yes Yes Yes(363452)Int. round b. 1.8 ml 50/500/2000 Yes Yes No Yes No Yes No(363401)Int. skirt b. 1.8 ml 50/450/1800 Yes Yes No Yes No Yes No(368632)Int. skirt b. 1.0 ml 50/500/2000 No Yes No Yes No Yes No(366656)Int. star b. 3.6 ml 50/400/1600 Yes Yes Yes Yes Yes Yes Yes(379189)Int. Star b. 4.5 ml 50/300/1200 Yes Yes Yes Yes Yes Yes Yes(379146)Int. star b. 1.8ml 50/450/1800 Yes Yes No Yes No Yes No(377267)Ext. star b. 1.8 ml 50/450/1800 Yes Yes No Yes No Yes No(375418)Ext. star b. 4.5 ml 50/300/1200 Yes Yes Yes Yes Yes Yes Yes(337516)Ext. round b. 1.8 ml 50/500/2000 Yes Yes No Yes N0 Yes No(340711)* Ink-jet printing directly on tube, special specifications.** If check digit is omitted, we can offer 9 counting digits in the code and the first digit as a prefix giving 10 digits.Interleaved2 of 5 Max13 digitsLarge labelCustom ServicesBarcoded FlasksNunc Brand Products offer two standard barcoded flasksintended for all users of the SelecT fully automated cell culturerobot, from The Automation Partnership. These flasks are alsouseful for all barcoded flask applications. The flask comes witha high quality 10-digit numeric Code 128 barcode label.Barcoded Flasks NunclonΔPolystyrene. Sterile. White filter capsCat. No. 178983 132920Culture area, cm² 175 500Suggested working 68 200volume, mlUnits per pack/case 4/32 4/32Barcode ScannersTwo different versions of handheld plug and play barcodescanners are available. Please see Page 41 for details.183

Always with updated information - www.nuncbrand.comAdditional InformationContentsAdditional Information............................186Physical Properties ofNunc Brand Products................................187Well and Tube Geometry..........................188Chemical ResistanceChart Nunc Labware.................................190Chemical Resistance Thermanox........194Chemical Resistance for Nunc..............196Cell Culture Thermanox Coverslips.....196Bulletins........................................................197Tech Notes............................................ 197-198Product Reference List...............................199Keywords..................................................... 204

www.nuncbrand.com - Always with updated informationPhysical Properties of Nunc Brand ProductsMax. Use Temp (°C)TransparencyMicrowave-ability¹Auto-clavingGasSterilization³Dry HeatRadiationDisinfectantsSpecific GravityFlexibilityPermeability Coefficientml - mmUnits: sec - cm² - cmHg x10-¹⁰N₂ O₂ CO₂ACL 121 Opaque Marginal² Yes⁴ Yes No No Yes 1.43 rigid 0.2 0.4 1.6ETFE/ 150 Transluc Yes Yes Yes Yes No Yes 1.70 rigid – – –ECTFEFEP 205 Transluc Marginal² Yes Yes Yes No Yes 2.15 excel 20 60 135HDPE 120 Transluc No No Yes No Yes Yes 0.95 rigid 3 10 45LDPE 80 Transluc Yes No Yes No Yes Yes 0.92 excel 20 60 280NYL 90 Transluc No No Yes No Yes Yes 1.13 rigid 0.3 1.0 1.8PC 135 Clear Marginal² Yes⁴ Yes No Yes Yes 1.20 rigid 3 20 85PETG 70 Clear Yes No Yes No Yes Yes 1.27 mod 0.8 1.1 4.5PFA 250 Transluc Yes Yes Yes Yes No Yes 2.15 excel – – –PMMA 50 Clear – No Yes No Yes Some 1.20 rigid – – –PP 135 Transluc Yes Yes Yes No No Yes 0.90 rigid 4 25 90PPCO 121 Transluc Marginal² Yes Yes No No Yes 0.90 mod 6 30 100PS 90 Clear No No Yes No Yes Some 1.05 rigid 3 15 75PSF 165 Clear Yes Yes Yes Yes Yes Yes 1.24 rigid 3 15 60PUR 82 Clear No No Yes No Yes Yes 1.20 excel – – –PVDF 110 Transluc – No Yes No No Yes 1.75 rigid – – –TFE 121 Opaque – Yes Yes No – Some 1.20 excel – – –TPE 260 Opaque – Yes Yes No – Some 1.20 excel – – –TPX 175 Clear Yes Yes Yes Yes No Yes 0.83 rigid 65 270 –XLPE 100 Transluc No No Yes No Yes Yes 0.93 rigid – – –TMX 150PMX 180Additional Information1 Ratings are based on 5-minute tests at 100% power (600watts) of exposed, empty labware. CAUTION: Do notexceed Max. Use Temp., above, or expose labware tochemicals where heating may cause to attack the plasticor be rapidly absorbed.2 Plastic will absorb heat.3 Sterilization:Autoclaving (121°C, 15 psi for 20 minutes) - Cleanand rinse item with distilled water before autoclaving.Certain chemicals, which have no appreciable effect onresins at room temperature, may cause deterioration atautoclaving temperatures unless removed with distilledwater beforehand. (Always completely disengage threadsbefore autoclaving).Gas - Ethylene oxide formaldehyde. Dry head (160%°C,120 minutes) Disinfectants - Benzalkonium chloride,formalin, ethanol, etc. Radiation - gamma irradiation at2.5 (Mrad) with unstabilized plastic.4 Sterilizing reduces mechanical strength. Do not usePC vessels for vacuum applications if they have beenautoclaved.ResinsThe table is meant a guideline only. The properties of the materials mayvary both due to minor differen ces in the production conditions and todifferent conditions in the testing laboratory. Therefore Nunc cannotguarantee that the specifications will always hold.All plastics have a limited tempe rature range as shown in the adjacent table.Resin Brittleness T⁰* Max. Use T⁰High density polyethylene (HDPE) -100 120Low density polyethylene (LDPE) -100 80Polycarbonate (PC) -135 135Polyethylene terephthalate copolymer (PETG) -40 70Permanox (PMX) -10 82Polypropylene Homopolymer (PP) 0 135Polypropylene Copolymer (PPCO) -40 121Polystyrene (PS) 20 90Thermanox (TMX) -60 65* The brittleness temperature is the temperature at which an item made from the resinor crack if dropped. This is not the lowest use temperature if care is exercised in use anhandling.185

Always with updated information - www.nuncbrand.comWell and Tube GeometryCorresponding figures relevant for designing solid phase assays in Nunc products.Additional InformationProductLiquidvolume µlCoveredarea mm²LiquidheightmmArea/volumeratio cm²/cm³C8 Break Apart Modules250 190 8.5 7.6300 µl200 159 6.9 7.9175 143 6.1 8.2150 127 5.2 8.5125 110 4.4 8.84.3 mm 100 94 3.5 9.475 78 2.7 10.450 61 1.8 12.2NucleoLink Modules330 234 11.2 7.1200 159 8.1 7.9330 µl100 96 5.2 9.650 57 2.4 11.425 37 1.6 14.8C8 StarWell Modules250 23 7.7 9.0200 198 6.3 9.9330 µl175 193 5.7 11.0150 166 4.9 11.0125 146 4.2 11.7100 125 3.4 12.54.1 mm75 100 2.6 13.350 72 1,8 14.4TSP in MicroWell F250 95 9.7 3.8200 75 7.6 3.8150 53 5.6 3.5125 41 4.6 3.3100 29 3.5 2.91 mm75 20 2.8 2.450 9 1.8 1.850 55 3.4 11.0Immuno Stick1800 µl4.3 mm10.0 mm11.2 mm11.2 mmVolume intubes µlPaddleareacoveredLiquidheighton paddlemm1000 520 8.5 (0.7) 5.2500 335 10,5 (0.7) 6.7250 176 6.2 (0.7) 7.0Area/volumecm²/cm³ProductLiquidvolume µlCoveredarea mm²LiquidheightmmArea/volumeratio cm²/cm³C8 LockWell Modules250 188 7.9 7.5200 157 6.4 7.8350 µl175 141 5.6 8.1150 126 4.8 8.4125 110 4.0 8.8100 94 3.2 9.44.6 mm 75 78 2.4 10.450 62 1.6 12.5U8 LockWell Modules250 178 8.6 7.1200 147 7.1 5.9320 µl175 132 6.3 7.5150 116 5.6 7.7125 101 4.8 8.0100 85 4.0 8.575 69 3.2 9.250 53 2.5 10.7C8 LockWell StarWell Modules250 244 8.4 9.8200 213 6.9 10.7330 µl175 196 6.2 11.2150 177 5.4 11.8125 156 4.5 12.5100 132 3.7 13.24.1 mm 75 105 2.8 14.050 75 1.9 15.1C8, C12 MicroWell Modules250 185 8.3 7.4200 154 6.7 7.7350 µl150 122 5.1 8.1125 106 4.3 8.5100 90 3.5 9.075 73 2.7 9.750 56 1.8 11.26.5 mm1536 Well Plates13 µl1.5 mm5 mm10.4 mm 11.0 mm11.0 mm11.2 mm12 32 4.6 26.710 27 3.9 27.38 23 3.2 28.06 18 2.4 29.34 113 1.6 31.52 8 0.9 37.31 5 0.5 47.2186

www.nuncbrand.com - Always with updated informationProductLiquidvolume µlCoveredarea mm²LiquidheightmmArea/volumeratio cm²/cm³96 DeepWell Plates 1.0 ml1200 649 26.9 5.41320 µl1000 553 23.2 5.5800 453 19.2 5.7600 350 15.1 5.8400 245 10.7 6.1200 138 6.1 6.9100 84 3.7 8.496 DeepWell Plates 2.0 ml1800 952 39.1 5.31935 µl1600 855 35.4 5.31200 658 27.7 5.51000 558 23.7 5.6600 354 15.3 5.9400 247 10.8 6.2200 136 6.0 6.8384 DeepWell Plates225 244 18.1 10.9245 µl200 220 16.4 11.0150 168 12.7 11.2125 142 10.8 11.4100 116 8.8 11.650 62 4.7 12.425 35 2.6 14F96, F16, F8 MicroWell Plates and Modules250 184 7.3 7.4350 µl200 154 5.9 7.7150 124 4.5 8.3125 109 3.8 8.7100 94 3.0 9.475 79 2.3 10.56.5 mm50 63 1.5 12.6Immuno Tube 70 x 111500 760 27.0 5.11000 520 18.4 5.2750 400 14.0 5.3500 280 9.5 5.4300 160 6.1 5.5250 140 5.6 5.61 5 0.5 47.2Immuno Tube 75 x 12 (Startube 75 x 12)1500 695 (815) 24.4 (25.5) 4.6 (5.4)1000 480 (600) 16.9 (18.0) 4.8 (6.0)500 260 (380) 9.3 (10.4) 5.2 (7.6)350 195 (315) 7.0 (7.6) 5.6 (9.0)300 175 (290) 6.2 (6.8) 5.8 (9.7)350 µl 250 155 /260) 5.4 (5.8) 6.2 (10.4)41.5 mm 29.1 mm20.0 mm11.2 mmLiquidheightmmProductLiquidvolume µlCoveredarea mm²U96, U16, U8 MicroWell Plates and Modules 0.35 ml250 184 9.0 7.4200 154 7.6 7.7350 µl150 124 6.2 8.3125 109 5.3 8.7100 94 4.7 9.475 78 3.9 10.450 63 3.2 12.6U96, U16, U8 MicroWell Plates and Modules 0.50 ml450 259 10.5 5.8400 234 9.6 5.9490 µl300 184 7.6 6.1200 134 5.6 6.7150 109 4.6 7.3100 84 3.5 8.450 55 2.3 11.0V96 MicroWell Plates 0.30 ml250 177 9.2 7.1200 147 7.8 7.3300 µl150 117 6.4 7.8125 101 5.6 8.1100 86 4.9 8.675 71 4.1 9.450 55 3.4 11.0V96 MicroWell Plates 0.45 ml400 234 10 5.9300 187 8.2 6.2455 µl250 161 7.2 6.4200 136 6.2 6.8150 111 5.2 7.4100 86 4.2 8.650 59 3.1 11.8384 Well Plates110 144 10.5 13.1126 µl100 133 9.7 13.280 110 8.0 13.760 86 6.3 14.340 61 4.4 15.32.9 mm20 36 2.3 17.610 32 1.2 21.9ShallowWell Plates22.5 38.5 4.8 17.120.0 35.5 4.5 17.625 µl15.0 28.6 3.8 19.112.5 25.2 3.4 20.210.0 21.5 3.0 21.55.0 13.0 1.9 26.02.5 7.8 1.1 31.210.3 mm11.2 mm9.8 mm11.0 mm11.6 mm5.2 mmArea/volumeratio cm²/cm³Additional Information187

Always with updated information - www.nuncbrand.comChemical Resistance Chart Nunc LabwareAdditional InformationInterpretation ofChemical ResistanceThe Chemical Resistance Chart thatfollows is a general guide only. Becauseso many factors can affect the chemicalresistance of a given product, you shouldtest under your own conditions.Effects of Chemicals on PlasticsChemicals can affect the strength,flexibility, surface appearance, color,dimensions or weight of plastics. Thebasic modes of interaction which causethese changes are: (1) chemical attackon the polymer chain, with resultantreduction in physical properties,including oxidation; reaction offunctional groups in or on the chain;and depolymerization; (2) physicalchange, including absorption of solvents,resulting in softening and swelling of theplastic; permeation of solvent throughthe plastic; dissolution in a solvent; and(3) stress cracking from the interactionof a “stress cracking agent” with internalor external stresses. Also see “ChemicalResistance Classification.”Mixing and/or dilution of certain chemicalsin Nunc labware can be potentiallydangerous.The reactive combination of differentchemicals or compounds of two ormore classes may cause an undesirablechemical effect or result in an increasedtemperature, which can affect chemicalresistance (as temperature increases,resistance to attack decreases). Otherfactors affecting chemical resistanceinclude pressure and internal or externalstresses (e.g. centrifugation), lengthof exposure, and concentra tion of thechemical.EnvironmentalStress CrackingEnvironmental stress cracking is the failureof a plastic material in the presenceof certain types of chemicals.This failure is not a result of chemicalattack. Simultaneous presence of threefactors causes stress cracking: tensilestress, a stress cracking agent and theinherent susceptability of the plastic tostress cracking.Common stress cracking agents aredetergents, surface active chemicals,lubricants, oils, ultra-pure water andplating additives such as brighteners andwetting agents. Relatively small concentrationsof stress cracking agent may besufficient to cause cracking.Mixing and/or dilution of certain chemicalsmay result in reactions whichproduce heat, which can cause productfailure.Pre-test your specific usage and alwaysfollow correct lab safety procedures.CautionDo not store strong oxidizing agents in plasticlabware except that made of TEFLON FEP orPFA. Prolonged exposure causes the material tobecome brittle.We have included a number of resins, which arenot used in Nunc Brand products, as we feel thatcustomers may find the information useful.Resin Codes:ACL Acetal (polyoxymethylene)ECTFE Halar ECTFE (ethylene-chlorotrifluoroethylenecopolymer)ETFE Tefzel ETFE (ethylene-tetrafluoroethylene)FEP TEFLON FEP (fluorinated ethylene propylene)HDPE High-density polyethyleneLDPE Low-density polyethyleneNYL Nylon (polyamide)PC PolycarbonatePETG Polyethylene terephthalate copolymerPFA TEFLON PFA (perfluoroalkoxy)PMMA Polymethyl methacrylate (acrylic)Resin Codes:PPPolypropylenePPCO* Polypropylene copolymerPSPolystyrenePSF PolysulfonePUR PolyurethanePVDF Polyvinylidene fluorideTFE TEFLON TFE (tetrafluoroethylene)TMX ThermanoxTPE Thermoplastic elastomerPMX PermanoxXLPE Cross-linked high-density polyethylene*PPCO has replaced polyallomer (PA) in all products.188

www.nuncbrand.com - Always with updated informationChemical Resistance ChartChemical Resistance Classifications are given at the bottom of the following charts.First letter of each pair applies to conditions at 20°C; the second to those at 50°C. At 20°C → EG ← at 50°C.Chemical LDPE HDPE PP PPCO PETG FEP TFE PFA ECTFE ETFE PC PSF PS PVDF PMX TMX1,2,4-Trichlorobenzene NN NN NN NN NN EE EE EE EG EG NN NN NN EE GF –1,2 Dichloroethane NN NN NN NN NN EE EE EE EE EE NN NN NN EE NN –1,4-Dioxane GF GG GF GF – EE EE EE EF EF GF GF NN NN GF EN2,2,4-Trimethylpentane FN FN FN FN – EE EE EE EG EG NN GF NN EE FN –2,4 Dichlorophenol NN NN NN NN – EE EE EE EE EE NN NN NN EE FN –2-Butanol EE EE EE EE – EE EE EE EE EE EG EG GG EE EG EN2-Methoxyethanol EG EE EE EE – EE EE EE EG EG NN NN NN EE EE –2-Propanol EE EE EE EE – EE EE EE EE EE EE EE EG EE EE ENAcetaldehyde GN GF GN GN – EE EE EE GF GF FN NN NN EE GN –Acetamide, Sat. EE EE EE EE – EE EE EE EE EE NN NN EE – EE EGAcetic Acid, 5% EE EE EE EE G- EE EE EE EE EE EG EE EG EE EE ENAcetic Acid, 50% EE EE EE EE FN EE EE EE EG EG EG GG GG EE EE ENAcetic Acid, Glacial EG EE EG EG NN EE EE EE EE EE NN FN NN EG EG ENAcetic Anhydride NN FF GF GF – EE EE EE EE EE NN NN NN NN EG –Acetone NN NN EG EE NN EE EE EE GF GF NN NN NN NN EE EFAcetonitrile EE EE FN FN – EE EE EE EE EE NN NN NN EE FN –Acetophenone NN FF FF FN – EE EE EE EE EE NN NN NN NN GN –Acrylonitrile EE EE FN FN – EE EE EE EG EG NN NN NN GF FN E -Adipic Acid EG EE EE EE – EE EE EE EE EE EE GG EE EE EE –Allyl Alcohol EE EE EE EE – EE EE EE EE EE GF GF GF EG EG ENAluminum Hydroxide EG EE EG EG – EE EE EE EE EE FN GG GG EE EG –Amino Acids EE EE EE EE – EE EE EE EE EE EE EE EE EE EE –Ammonia EE EE EE EE – EE EE EE EE EE NN GF GF EE EE NNAmmonia, 25% EE EE EE EE – EE EE EE EE EE NN EG EG NN EE NNAmmonium Glycolate EG EE EG EG – EE EE EE EE EE GF GG EE EE EG –Ammonium Hydroxide, EE EE EE EE FN EE EE EE EE EE FN GG EF EE EE FN5%Ammonium Hydroxide, EG EE EG EG NN EE EE EE EE EE NN GG GF EE EG NN30%Ammonium Oxalate EG EE EG EG – EE EE EE EE EE EE EE EE EE EG –Ammonium Salts EE EE EE EE – EE EE EE EE EE EG EE GG EE EE GFAmyl Alcohol EE EE EE EE – EE EE EE EE EE GF EE GF EE EE E -Amyl Chloride NN FN NN NN – EE EE EE EE EE NN NN NN EE NN –Aniline EG EG GF GF – EE EE EE GN GN FN NN NN EF GF E -Aqua Regia NN NN NN NN – EE EE EE EG EG NN NN NN EF NN NNArsenic Acid GF EG EE EG – EE EE EE EE EE EE – EE EE EE –Benzaldehyde EG GN EG EG – EE EE EE EF EF FN FF NN EE EG E -Benzenamine EG EG GF GF – EE EE EE GN GN FN NN NN EF GF E -Benzene NN NN NN NN NN EE EE EE EG EG NN NN NN EE GF GNBenzoic Acid, Sat. EE EE EG EG – EE EE EE EE EE EG FF GG EE EG ENBenzyl Acetate EG EE EG EG – EE EE EE EG EG FN NN NN – EG –Benzyl Alcohol NN FN NN NN – EE EE EE EE EE NN NN NN EE NN EFBoric Acid EE EE EE EE NN EE EE EE EE EE EE – EG EE EE –Bromine NN FN NN NN – EE EE EE EG EG FN NN NN EE NN –Bromobenzene NN NN NN NN – EE EE EE GN GN NN NN NN EE NN –Bromoform NN NN NN NN – EE EE EE GF GF NN NN NN EE NN –Butadiene NN FN NN NN – EE EE EE EE EE NN NN NN EE NN –Butyl Acetate NN FF FF FF – EE EE EE EE EE NN NN NN GN GF G-Butyl Chloride NN NN NN NN – EE EE EE EE EE NN NN NN EE FN –Butyric Acid NN FN NN NN – EE EE EE EE EE FN GG NN EE NN –Additional InformationE - No damage after 30 days of constant exposure. G - Little or no damage after 30 days of constant exposure.F - Some effect after 7 days of constant exposure. N - Immediate damage may occur. Not recommended for continuous use.189

Always with updated information - www.nuncbrand.comChemical Resistance ChartChemical Resistance Classifications are given at the bottom of the following charts.First letter of each pair applies to conditions at 20°C; the second to those at 50°C. At 20°C → EG ← at 50°C.Additional InformationChemical LDPE HDPE PP PPCO PETG FEP TFE PFA ECTFE ETFE PC PSF PS PVDF PMX TMXCalcium Hydroxide EE EE EE EE – EE EE EE EE EE NN GG GG EE EE –Calcium Hypochlorite EE EE EE EE – EE EE EE EE EE FN EE GF EE EG NNCarbazole EE EE EE EE – EE EE EE EE EE NN NN EE – EE –Carbon Disulphide NN NN NN NN – EE EE EE EF EF NN NN NN EE NN E -Carbon Tetrachloride FN GF GF GF NN EE EE EE EE EE NN NN NN EE NN NNCellosolve Acetate EG EE EG EG – EE EE EE EG EG FN NN NN EG EG –Chlorine Water GN GF FN FN – EE EE EE EE EE GF NN NN EE GF NNChlorine, 10% (Moist) GN GF FN FN – EE EE EE EE EE GF NN NN EE GN NNChlorine, 10% in air GN EF GN GN – EE EE EE EE EE EG NN FN EE GN NNChlorine, wet gas GN GF FN FN – EE EE EE EE EE GF NN NN EE GN NNChloroacetic Acid EE EE EG EG – EE EE EE EE EE FN NN GN E - EG –Chlorobenzene NN NN NN NN – EE EE EE EG EG NN NN NN EE FN GNChloroform FN FN NN NN – EE EE EE GF GF NN NN NN EE NN N -Chromic Acid, 10% EE EE EE EE – EE EE EE EE EE GF NN EE EE EE NNChromic Acid, 20% EE EE GG GF – EE EE EE EE EG GF NN GG EG EE NNChromic Acid, 50% EE EE GF GF – EE EE EE EE EE FN NN FF EG GF NNChromic:Surfuric Acid NN NN NN NN E - EE EE EE EG EG NN NN NN GN NN NNMixture, 96%Citric Acid, 10% EE EE EE EE – EE EE EE EE EE EG EE EG EE EE –Cresol NN FN GF GF – EE EE EE EG EG NN NN NN EE NN ENCyclohexane FN FN FN FN – EE EE EE EG EG EG NN NN EE NN –Cyclohexanone NN FN FN FN NN EE EE EE EE EE NN NN NN FN GF EFCyclopentane NN FN FN FN – EE EE EE EE EE NN NN NN EE FN –Decahydronaphtalene GF EG GF GF – EE EE EE EE EE NN NN NN – FN –Diacetone NN NN GF GF – EE EE EE GF GF NN NN NN GN FF E -Diacetone Alcohol FN EE EF EF – EE EE EE EG EG NN NN GN NN EE –Dibutylphthalate – - N NN – – EE EE EE GN EG GN NN NN GN GG FNDiethyl Benzene NN FN NN NN – EE EE EE EG EG FN NN NN EE NN –Diethyl Ether NN FN NN NN – EE EE EE EG EG NN NN NN EG NN ENDiethyl Ketone NN NN GG GG – EE EE EE GF GF NN NN NN NN GF E -Diethyl Malonate EE EE EE EE – EE EE EE EE EE FN FF NN EG EG –Diethylamine NN FN GN GN – EE EE EE EG EG NN GF GG NN FF –Diethylene Dioxide GF GG GF GF – EE EE EE GG EG FN NN NN NN FN ENDiethylene Glycol EE EE EE EE – EE EE EE EE EE GF GG GG EE EE –Diethylene Glycol Ethyl EE EE EE EE – EE EE EE EE EE FN FF NN NN EE –EtherDimethyl Acetamide FN EE EE EE – EE EE EE EG EG NN NN NN NN FG ENDimethyl Formamide EE EE EE EE – EE EE EE GG GG NN NN NN NN EE ENDimethylsulphoxide EE EE EE EE NN EE EE EE EG EG NN NN EG NN EE EN(DMSO)Dioxane GF GG GF GF – EE EE EE GG EG FN NN NN NN FN ENDimethyl Formamide EE EE EE EE – EE EE EE GG GG NN NN NN NN EE ENDimethylsulphoxide EE EE EE EE NN EE EE EE EG EG NN NN EG NN EE EN(DMSO)Dioxane GF GG GF GF – EE EE EE GG EG FN NN NN NN FN ENDipropylene Glycol EE EE EE EE – EE EE EE EE EE GF GG EE NN EE –Ethanol, 40% EG EE EG EG – EE EE EE EE EE EG EG GF EE EG ENEther NN FN NN NN E - EE EE EE EG EG NN NN NN EG NN ENEthyl acetate EE EE EG EE NN EE EE EE EE EE NN NN NN NN FN ENEthyl Alcohol (Absolute) EG EE EG EG – EE EE EE EE EE EG EG FN EE EG ENE - No damage after 30 days of constant exposure. G - Little or no damage after 30 days of constant exposure.F - Some effect after 7 days of constant exposure. N - Immediate damage may occur. Not recommended for continuous use.190

www.nuncbrand.com - Always with updated informationChemical Resistance Classifications are given at the bottom of the following charts.First letter of each pair applies to conditions at 20°C; the second to those at 50°C. At 20°C → EG ← at 50°C.Chemical LDPE HDPE PP PPCO PETG FEP TFE PFA ECTFE ETFE PC PSF PS PVDF PMX TMXEthyl Alcohol, 40% EG EE EE EE G - EE EE EE EE EE EG EG GF EE EG ENEthyl Alcohol, 96% EG EG EE EE G - EE EE EE EE EE EG EG GF EE EG –Ethyl Benzene NN NN NN NN E - EE EE EE GF GF NN NN NN – NN –Ethyl Benzoate FF GG GF GF – EE EE EE EG EG NN NN NN NN GF –Ethyl Butyrate GN GF GN GN – EE EE EE EG EG NN NN NN NN FN –Ethyl Chloride FN FF FN FN – EE EE EE EE EE NN NN NN EE FN –Ethyl Chloride, Liquid FN FF FN FN – EE EE EE EE EE NN NN NN EE FN –Ethyl Cyanoacetate EE EE EE EE – EE EE EE EE EE FN FF GN NN EE –Ethyl Lactate EE EE EE EE – EE EE EE EE EE FN FF FN NN EE –Ethylene Chloride GN GF FN FN NN EE EE EE EE EE NN NN NN EE NN EFEthylene Glycol EE EE EE EE E - EE EE EE EE EE GF EE EE EE EE –Ethylene Glycol EE EE EE EE – EE EE EE EE EE FN FF NN NN EE –Monomethyl Ether FF GF FF FF GF EE EE EE EE EE FN EE NN EE FN –Ethylene Oxide Gas FF GF FF FF GF EE EE EE EE EE FN EE NN EE FN –Ethylene Oxide, 100% EG EE EG EG GF EE EE EE EG EG GF GG EF EE EG –Fatty Acids FN GN FN FN – EG EG EG EF EF GF NN NN GN FN E -Fluorine EE EE EE EE – EE EE EE EE EE EG GF FN EE EG E -Formaldehyde, 10% EG EE EG EG – EE EE EE EE EE EG GF NN EE EG E -Formaldehyde, 40% EE EE EE EE – EE EE EE EE EE EG GF FN EE EG E -Formalin, 10% EG EE EG EG – EE EE EE EE EE EG GF NN EE EG GNFormalin, 40% EG EE EG EG – EE EE EE EE EE EF FF FF EE EF ENFormic Acid EG EE EG EG – EE EE EE EE EE EG GG EG EE EG ENFormic Acid, 3% EG EE EG EG – EE EE EE EE EE GF GG FF EE EG GNFormic Acid, 50% EE EE EG EG – EE EE EE EE EE FN EG FF EE EF GNFormic Acid, 85% EG EE EG EG – EE EE EE EE EE FN FF FF EE EF E -Formic Acid, 100% EG EG EG EG – EE EE EE EG EG GF EG FN EE FN E -Freon TF EG EE EE EE G - EE EE EE EG EG EF GG EF EE FF EFGlutaraldehyde EE EE EE EE – EE EE EE EE EE EE EE EE EE EE E -Glycerine (Glycerol) NN GF GF GF E - EE EE EE EE EE FN EG NN EE FN –Hexane NN NN NN NN – EE EE EE GF GF NN NN NN NN NN GNHydrazine – - N EG – – EE EE EE EE EE GF EG FF EE EE GFHydrobromic Acid, 69% EE EE EE EE – EE EE EE EE EE EE EE EE EE EG GNHydrochloric Acid, 5% EE EE EE EE E - EE EE EE EE EE GF EE EE EE EG NNHydrochloric Acid, 20% EE EE EG EG – EE EE EE EE EE NN EE FF EE EG GFHydrochloric Acid, 35% EG EE EG EG FN EE EE EE EE EE GF GF GF EE EG GNHydrobromic Acid, 4% EE EE EE EE – EE EE EE EE EE NN FN NN EE EE EFHydrobromic Acid, 48% EE EE EE EE – EE EE EE EE EE EE EE EG EE EE ENHydrogen Peroxide, 3% EG EE EG EG E - EE EE EE EE EE EE EE EG EE EG GNHydrogen Peroxide, 30% EG EE EG EG E - EE EE EE EE EE EE EE EG E - EG –Hydrogen Peroxide, 90% NN NN FN FN – EE EE EE EG EG NN NN NN EE GN ENLodine Crystals EE EE EE EE – EE EE EE EE EE EG EG GG EE EG ENIsobutanol EE EE EE EE – EE EE EE EE EE EE EE EG EE EE –Isopropanol, 100% GF EG GF GF – EE EE EE EG EG NN NN NN GN GF –Isopropyl Acetate FN GF FN FN – EE EE EE EG EG NN NN NN – NNIsopropyl Ether NN NN NN NN – EE EE EE EG EG NN NN NN EE EE –Lactic Acid, 3% EG EE EG EG FN EE EE EE EE EE EG EE GG EG EG ENLactic Acid, 85% EG EE EG EG NN EE EE EE EG EG EG EE GG GF EG GNMercury EE EE EE EE – EE EE EE EG EG NN EE EE EE EE –Methanol, 100% EE EE EE EE G - EE EE EE EE EE GF GF FN EE EE ENMethoxyethyl Oleate EG EE EG EG G - EE EE EE EE EE FN NN NN – EG –Additional InformationE - No damage after 30 days of constant exposure. G - Little or no damage after 30 days of constant exposure.F - Some effect after 7 days of constant exposure. N - Immediate damage may occur. Not recommended for continuous use.191

Always with updated information - www.nuncbrand.comChemical Resistance ChartChemical Resistance Classifications are given at the bottom of the following charts.First letter of each pair applies to conditions at 20°C; the second to those at 50°C. At 20°C → EG ← at 50°C.Additional InformationChemical LDPE HDPE PP PPCO PETG FEP TFE PFA ECTFE ETFE PC PSF PS PVDF PMX TMXMethyl Acetate FN FF GF GF – EE EE EE EG EG NN NN NN NN EE –Methyl Ethyl Ketone NN NN EG EG G - EE EE EE GF GF NN NN NN NN NN E -Methyl Isobutyl Ketone NN NN GF GF NN EE EE EE GF GF NN NN NN GN FF E -(MIBK)Methyl Propyl Ketone GF EG GF GF NN EE EE EE EG EG NN NN NN NN FF E -Methyl-t-Butyl Ether NN FN FN FN NN EE EE EE EG EG NN NN NN EE EE –Methylene Chloride FN FN FN FN NN EE EE EE GG GG NN NN NN NN FN NNNitric Acid, 10% EE EE EE EE G - EE EE EE EE EE EG EF GN EE EE GNNitric Acid, 20% EG GF FF GF G - EE EE EE EE EE GF GF NN EF GF FNNitric Acid, 50% GN GN FN FN G - EE EE EE EE EE GF GF NN EG FN NNNitric Acid, 70% FN GN NN NN NN EE EE EE EE EE NN NN NN GF FN NNNitrobenzene NN FN NN NN NN EE EE EE EG EG NN NN NN EN NN GNNitromethane NN FN FN FN NN EE EE EE EF EF NN NN NN GF EF –n-Amyl Acetate GF EG GF GF – EE EE EE EE EE NN NN NN EE GF E -n-Butanol EE EE EE EE – EE EE EE EE EE GF GF EG EE EG ENn-Butyl Acetate GF EG GF GF – EE EE EE EG EG NN NN NN EE GF –n-Decane FN FN FN FN – EE EE EE EE EE FN GF FN EE FN –n-Heptane FN GF FF FF – EE EE EE EE EE EG EG NN EE FF E -n-Octane EE EE EE EE – EE EE EE EE EE GF GF NN EE EE –Oil, Mineral GN EE EE EE – EE EE EE EE EE EG EE EE EE EG EEOxalic Acid, 10% EE EE EE EE – EE EE EE EG EE EE EE EE EF EE ENOzone EG EE EG EG – EE EE EE EE EE EG EE FF EE EE –o-Dichlorobenzene FN FF FN FN NN EE EE EE EF EF NN NN NN EE FN G -Perchloric Acid GN GN GN GN – GF GF GF EG EG NN NN GF EE GN –Perchloric Acid, 70% GN GN GN GN – GF GF GF EG EG NN NN GF EE GN –Perchloroethylene NN NN NN NN – EE EE EE EE EE NN NN NN EE NN GNPhenol, 100% NN NN NN NN NN EE EE EE EE EE NN NN NN GN NN NNPhenol, 50% NN NN NN NN NN EE EE EE EF EF NN NN FN EE NN E -Phenol, Crystals GN GF GN GN NN EE EE EE EE EE NN FF NN EE FG –Phenol, Liquid NN NN NN NN NN EE EE EE EF EF NN NN FN EE NN –Phosphoric Acid, 5% EE EE EE EE – EE EE EE EE EE EE EE GG EE EE GNPhosphoric Acid, 85% EE EE EG EG – EE EE EE EE EE EG EE EG EE EG NNPicric Acid NN NN NN NN – EE EE EE GF GF NN NN GF EE EE –Potassium Hydroxide, EE EE EE EE – EE EE EE EE EE FN EE GG EE EE GN1%Potassium Hydroxide, EE EE EE EE – EE EE EE EE EE NN EE GG GF EE NN30%Potassium Hydroxide, EE EE EE EE – EE EE EE EE EE NN EE GG EG EE NNConcentratedPotassiumEE EE EE EE – EE EE EE EE EE EE EE GF EE EE –PermanganatePropane NN FN NN NN – EE EE EE EE EE FN FF NN EE NN –Proprionic Acid FN EF EG EG – EE EE EE EF EF NN GG GN EE EF –Propylene Glycol EE EE EE EE – EE EE EE EE EE GF GG EE EE EE –Propylene Oxide EG EE EG EG – EE EE EE FN FN GF GG NN FN EG –Pyridine NN NN NN NN – EE EE EE NN EG NN NN NN NN NN GNp-Chloroacetophenone EE EE EE EE – EE EE EE EE EE NN NN NN NN EE –p-Dichlorobenzene FN GF GF GF – EE EE EE EF EE NN NN NN EE GF G -Resorcinol, 5% EE EE EE EE – EE EE EE EF EF GF NN GF EE EE E -Resorcinol, Sat. EE EE EE EE – EE EE EE EE EE GF NN GF EE EE E -Salicylaldehyde EG EE EG EG – EE EE EE EN EN GF FF NN EG EG –E - No damage after 30 days of constant exposure. G - Little or no damage after 30 days of constant exposure.F - Some effect after 7 days of constant exposure. N - Immediate damage may occur. Not recommended for continuous use.192

www.nuncbrand.com - Always with updated informationChemical Resistance Classifications are given at the bottom of the following charts.First letter of each pair applies to conditions at 20°C; the second to those at 50°C. At 20°C → EG ← at 50°C.Chemical LDPE HDPE PP PPCO PETG FEP TFE PFA ECTFE ETFE PC PSF PS PVDF PMX TMXSalicylic Acid, Sat. EE EE EE EE – EE EE EE EE EE EG EE EG EE EE –Salt Solutions, Metallic EE EE EE EE – EE EE EE EE EE EE EE GG EE EE EFSilicone Oil EG EE EE EE NN EE EE EE EE EE EE EE EG EE EE –Silver Nitrate EG EE EG EG – EE EE EE EE EE EE EE GF EE EE GNSodium Dichromate EE EE EE EE – EE EE EE EE EE EE EE EE EE EE –Sodium Hydroxide, 1% EE GF EE EE E - EE EE EE EE EE FN EE EE EE EE GNSodium Hydroxide, 10% EE GF EE EE E - EE EE EE EE EE NN EG EE EF EE NNSodium Hydroxide, 50% GG GF EE EE NN EE EE EE EE EE NN EG EG EG EE NNSodium Hypochlorite, EE EE GF GF G - EE EE EE EE EE GF EE EE EE EE NN15%Stearic Acid EE EE EE EE – EE EE EE EE EE EG GG EG EE EE –Sulfur dioxide NN FN NN NN – EE EE EE EG EG GN GG NN EE NN –Sulfur Dioxide, wet or EE EE EE EE – EE EE EE EE EE EG GG FN GE EE –drySulfur Salts FN GF FN FN – EE EE EE EG EG FN GG NN GF FN –Sulfuric Acid, 6% EE EE EE EE E - EE EE EE EE EE EE EE EG EE EE GNSulfuric Acid, 20% EE EE EG EG E - EE EE EE EE EE EG EE EG EE EG GNSulfuric Acid, 30% EE EE GG EG G - EE EE EE EE EE GF EE GN EE EG NNSulfuric Acid, 60% EG EE EG EG – EE EE EE EE EE GF EE GN EE EG NNSulfuric Acid, 98% GG GG FN FN NN EE EE EE EE EE NN NN NN EG GG NNSulfuric Acid, (96%) GG GG FN FN NN EE EE EE EE EE NN NN NN EG GG NNTartaric Acid EE EE EE EE – EE EE EE EE EE EG EE GG EE EE –Tetrahydrofuran FN GF GF GF – EE EE EE GF GF NN NN NN FN FF –Thionyl Chloride NN NN NN NN – EE EE EE EE EE NN NN NN NN NN –Tincture of Iodine EG EG GG GG – EE EE EE EE EE NN – GF EG NN –Toluene FN FN FN GF FN EE EE EE EE EE FN NN NN EE FF GNTributyl Citrate GF EG GF GF – EE EE EE EG EG NN FF NN EF GF –Trichloroacetic Acid FN FF FN FN – EE EE EE EF EF FN GG FN EG EE NN(TCA)Trichloroethane NN FN NN NN – EG EG EG NN NN NN NN NN EE NN GNTrichloroethylene NN FN NN NN – EE EE EE EG EG NN NN NN EE NN GNTriethylene Glycol EE EE EE EE – EE EE EE EE EE EG EE EG – EE –Tripropylene Glycol EE EE EE EE – EE EE EE EE EE EG EE EE – EE –Tris Buffer, Solution EG EG EG EG GG EE EE EE EE EE GF GF GN EG EG EEUrea EE EE EE EE – EE EE EE EE EE NN FF EG EE EE EEXylene GN GF FN FN – EE EE EE EG EG NN NN NN EE NN GGE - No damage after 30 days of constant exposure. G - Little or no damage after 30 days of constant exposure.F - Some effect after 7 days of constant exposure. N - Immediate damage may occur. Not recommended for continuous use.Additional Information193

Always with updated information - www.nuncbrand.comChemical Resistance ThermanoxAdditional InformationChemical Resistance for NuncCell Culture Thermanox CoverslipsNunc Brand Thermanox, or TMXcoverslips, are made of a polymer thatis highly resistant to most chemicals.Thermanox is resistant to alcohols,aldehydes, hydro carbons, diluteacids (

www.nuncbrand.com - Always with updated informationBulletinsTech NotesAll Bulletins and Tech Notes areavailable for download as pdffiles. Use the links given belowfor direct access to the files.Bulletin No. 1Edge Effect in MicroWell ELISA/Adsorption Geometry in Nunc Productsfor Solid Phase Assayswww.nuncbrand.com/go/b01Bulletin No. 2Evaporation from Tissue Culture Plates/Vibration Patterns in TissueCulture Vesselswww.nuncbrand.com/go/b02Bulletin No. 3Incubator Shelf “Images” in Monolayer Culture/Aspects of CryoPreservationwww.nuncbrand.com/go/b03Bulletin No. 4Aspects of Nunc MaxiSorp MicroWell Certification/Negative Edge Effectin MicroWell ELISAwww.nuncbrand.com/go/b04Bulletin No. 5pH and Pressure in Closed Tissue Culture Vesselswww.nuncbrand.com/go/b05Bulletin No. 6Principles in Adsorption to Polystyrene/Stability of Nunc-ImmunoMaxiSorp Surfaceswww.nuncbrand.com/go/b06Bulletin No. 7Comparison of Blocking Agents for ELISA/Nunc-Immuno Stick Methodswww.nuncbrand.com/go/b07Bulletin No. 8Detergents in Polystyrene ELISAwww.nuncbrand.com/go/b08Bulletin No. 9Blocking Agent and Detergent in ELISAwww.nuncbrand.com/go/b09Bulletin No. 10Covalent Binding of DNA to CovaLink NH, Methods and Applications/The Surface/Volume Ratio in Solid Phase Assayswww.nuncbrand.com/go/b10Bulletin No. 11Colorimetric Determination of Amino Groups of CovaLink NHMicroWells/Activity of Adsorbed Antibodieswww.nuncbrand.com/go/b11Bulletin No. 12Effects of Enlarged Surface/Volume Ratio in Solid Phase AssaysDocumented on Basis of Nunc StarWell Modules/Comparison of Affinity-Isolated and Non-Isolated Antibodies Used as Capture Antibodies inELISAwww.nuncbrand.com/go/b12Bulletin No. 13Cell Adhesion and Growth on Coated or Modified Glass or Plasticwww.nuncbrand.com/go/b13Bulletin No. 14Silent Screen Plate Design and Applicationswww.nuncbrand.com/go/b14Bulletin No. 15Substrate Performance Study of 96- and 384-well NUNC Polymer OpticalBottom Plateswww.nuncbrand.com/go/b15Tech Note, Vol. 1 No. 2Nunclon∆ TripleFlask Culturing Techniquewww.nuncbrand.com/go/tn01Tech Note, Vol. 1 No. 3Non-enzymatic Methods for Cell Harvestingwww.nuncbrand.com/go/tn02Tech Note, Vol. 1 No. 6FluoroNunc Modules and Plates - Nunc Q.C. Procedure and the PrincipleBehindwww.nuncbrand.com/go/tn06Tech Note, Vol. 1 No. 9Coupling of Oligosaccharides to Nunc CovaLink NH Moduleswww.nuncbrand.com/go/tn09Tech Note, Vol. 1 No. 10Performance of Nunc-Immuno C8 StarWellwww.nuncbrand.com/go/tn10Tech Note, Vol. 2 No. 11DIAPOPS using CovaLink BreakApart Moduleswww.nuncbrand.com/go/tn11Tech Note, Vol. 2 No. 12FluoroNunc Plates and Modules: A Solid Phase for Fluorescent ImmunoAssayswww.nuncbrand.com/go/tn12Tech Note, Vol. 2 No. 13Nunc Cell Culture Insertswww.nuncbrand.com/go/tn13Tech Note, Vol. 2 No. 14Cryopreservation of Mammalian Cells - Protocolswww.nuncbrand.com/go/tn14Tech Note, Vol. 3 No. 15Compatibility of Various Mounting Media on Permanox Slideswww.nuncbrand.com/go/tn15Tech Note, Vol. 3 No. 17NucleoLink versus CovaLink Surfaceswww.nuncbrand.com/go/tn17Tech Note, Vol. 3 No. 18Thermal Profiles of Liquid in NucleoLink and TopYield Strips and PerkinElmer thin wall Tubes in the Perkin Elmer 9600 Thermal Cyclerwww.nuncbrand.com/go/tn18Tech Note, Vol. 3 No. 19NucleoLink and TopYield Strips as Traditional Amplification Tubes inCommercial Thermal Cyclerswww.nuncbrand.com/go/tn19Tech Note, Vol. 3 No. 20Compatibility of Chamber Slide Components with Various FixationReagentswww.nuncbrand.com/go/tn20Tech Note, Vol. 3 No. 21Picking and Arraying Libraries with the Nunc Replication Systemwww.nuncbrand.com/go/tn21Tech Note, Vol. 3 No. 22Construction of High Density Array of Libraries on Agar or NylonMembraneswww.nuncbrand.com/go/tn22Bulletins/Tech Notes195

Always with updated information - www.nuncbrand.comTech NotesAll Bulletins and Tech Notes are availablefor download as pdf files. Use the links givenbelow for direct access to the files.Bulletins/Tech Notes196Tech Note, Vol. 3 No. 23Replication of Recombinant Libraries with the Nunc Replication Systemwww.nuncbrand.com/go/tn23Tech Note, Vol. 3 No. 24In Situ Screening of Bacterial Colonies - Protocolswww.nuncbrand.com/go/tn24Tech Note, Vol. 3 No. 25Cell Counting and Dye Exclusion Viability Assays Using a Hemacytometerwww.nuncbrand.com/go/tn25Tech Note, Vol. 4 No. 33Chemical Resistance for Nunc Cell Culture THERMANOX Coverslipswww.nuncbrand.com/go/tn4-33Tech Note, Vol. 4 No. 34Culturing HEL 299 Cell Line on a Nunclon∆ Cell Culture Treated Surfacewww.nuncbrand.com/go/tn4-34Tech Note, Vol. 4 No. 35Culturing L929 Cell Line on a Nunclon∆ Cell Culture Treated Surfacewww.nuncbrand.com/go/tn4-36Tech Note, Vol. 4 No. 36Culturing Primary Chick Embryo Cells on a Nunclon∆ Cell CultureTreated Surfacewww.nuncbrand.com/go/tn4-36Tech Note, Vol. 4 No. 37Culturing V79-4 Cell Line on a Nunclon∆ Cell Culture Treated Surfacewww.nuncbrand.com/go/tn4-37Tech Note, Vol. 5 No. 33Versatile PCR assays based on hybridization in MicroWell plateswww.nuncbrand.com/go/tn5-33Tech Note, Vol. 5 No. 34Streptavidin Coated Plates for Molecular Biologywww.nuncbrand.com/go/tn5-34Tech Note, Vol. 5 No. 35High Sensitivity Detection of Antigens using Immuno-PCRwww.nuncbrand.com/go/tn5-35Tech Note, Vol. 5 No. 36NucleoLink Procedure for Solid Phase-PCR (DIAPOPS)www.nuncbrand.com/go/tn5-36Tech Note, Vol. 5 No. 37NucleoLink Procedure for PCR-ELISAwww.nuncbrand.com/go/tn5-37Tech Note, Vol. 6 No. 38Cell-Based Assay Development: Detection of Active Apoptotic Caspases in96 Well Silent Screen Plates with PVM Membranewww.nuncbrand.com/go/tn38Tech Note, Vol. 6 No. 39Cost-efficient plasmid DNA purification using the Nunc 96 DeepWellDNA binding filter platewww.nuncbrand.com/go/tn39Tech Note, Vol. 6 No. 40A new optimized PCR clean up method using the Nunc glass fiber filterplatewww.nuncbrand.com/go/tn40Tech Note, Vol. 6 No. 41Nunc Immobilizer Streptavidin: Colorimetric detection of human IgG inblood plasmawww.nuncbrand.com/go/tn41Tech Note, Vol. 6 No. 42Nunc Immobilizer Streptavidin: PCR ELISAwww.nuncbrand.com/go/tn42Tech Note, Vol. 6 No. 43Nunc Immobilizer Amino Surface: Protocol for Coupling Proteinswww.nuncbrand.com/go/tn43Tech Note No. 44Nunc Immobilizer Glutathionewww.nuncbrand.com/go/tn44Tech Note No. 45Detection of glutathione-S-transferase (GST) and GST-tagged fusionproteinswww.nuncbrand.com/go/tn45Tech Note No. 46Nunc Immobilizer Nickel-Chelatewww.nuncbrand.com/go/tn46Tech Note No. 47Detection of His-tagged fusion proteinswww.nuncbrand.com/go/tn47Tech Note No. 48High Density Culture on 2D MicroHex Microcarrierswww.nuncbrand.com/go/tn48Tech Note No. 49Performance of Nunc Aluminium Sealing Tapes in Thermocycling andFrozen Storage Applicationswww.nuncbrand.com/go/tn49Tech Note No. 50Low DNA adsorption to Nunc Bank-It vialswww.nuncbrand.com/go/tn50Tech Note No. 51Low Cell Binding Surface - A Performance Comparison, Nunc Low CellBinding Plates vs. Competitor’s Ultra Low Attachmentwww.nuncbrand.com/go/tn51Tech Note No. 52Comparison of bottom materials in cell based assayswww.nuncbrand.com/go/tn52Tech Note No. 53Direct Measurement of DNA concentration and purity at 230, 260 and 280nm and in fluorescence modewww.nuncbrand.com/go/tn53Tech Note No. 54Comparative detection of tryptophan in absorbance and fluorescencespectrometrywww.nuncbrand.com/go/tn54Tech Note No. 55Imaging optimization with appropriate cover class correctionwww.nuncbrand.com/go/tn55Tech Note No. 56Binding DNA on Immobilizer Aminowww.nuncbrand.com/go/tn56Tech Note No. 57Growth of Vero cells in 1.2x In Vitro PETG Roller Bottles using 5% FetalBovine Serumwww.nuncbrand.com/go/tn57Tech Note No. 58C8 White LockWell Luminunc MaxiSorp and PolySorp for LuminescenceDetectionwww.nuncbrand.com/go/tn58Tech Note No. 59C8 Black LockWell Fluoronunc MaxiSorp and PolySorp for FluorescenceDetectionwww.nuncbrand.com/go/tn59Tech Note No. 60Odor from irradiated polystyrene has no effect on embryo and spermsurvivalwww.nuncbrand.com/go/tn60Tech Note No. 61Imaging of intracellular oxygen consumption for assessment ofmitochondrial function in live suspension cell lines: Novel applicationusing Luxcel MitoXpress probes and the Nunc LiveCell Array slidewww.nuncbrand.com/go/tn61165306

www.nuncbrand.com - Always with updated informationProduct Reference ListPages listed in parentheses containreferences to the Cat. No.Cat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page4014 1264021 1264022 1264031 1264036 126130504 28130505 28130506 28130507 28130508 28130509 28130510 28130511 28130512 28130513 28130514 28130515 28130516 28130517 28130518 28130519 28132703 11132704 11132705 11132706 11132707 11132708 11132744 136132867 15132869 134132903 13132913 15132920 15, (14, 183)133001 11133002 11136101 147136102 147136196 12136528 29136730 24136935 24137044 24137052 25137060 25137101 147137103 147137370 25137435 25137443 25137508 25139102 139139104 139139106 139139108 139139446131, (136,137)140000 130140004 131140082 134140099 130, 134140156 16140175 16140224 137140250 130140360 130140400 130, (136)140620 23140627 23140629 23140640 23140642 23140644 23140652 23140654 23140656 23140660 23140663 23140668 23140675 20140685 20142475 20142485 20142761 164142762 164143761 150144444 85144458 14144530 21144652 14144881 14144903 13145470 29146003 14146008 130146183 29147074 14147104 14150067 27, (196)150200 21150239 21150255 85150270 85150288 26150318 26150326 26150340 26150350 26150360 85150628 20150679 26150687 20150787 21151152 14151209 14152028 152152029 164152034 20152035 20152036 152152037 152152038 145152039 145152040 152152041 164152094 12152640 20153066 26153732 12154453 31154461 31154526 31154534 31154739 31154852 31154917 31154941 31155330 18155331 18155332 18155333 18155334 18155335 18155336 18155337 19155338 19155339 19155340 19155342 19155343 19155344 19155360 32155361 32155379 32155380 32155382 32155383 32155409 32155411 32156340 11156367 11156472 11156499 11156502 12156545 145156753 13156758 29156800 13157150 26157527 11158240 11158523 11158892 11159609 17159617 17159625 17159633 17159641 17159668 17159910 11159920 11159926 13159929 13159933 11159934 11160004 27160005 27160376 153161093 145161395 24162243 24163118 29Product Reference List197

Always with updated information - www.nuncbrand.comProduct Reference ListProduct Reference ListCat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page163320 150163371 12164310 163164327 131, 136, 137164400 163164555 161164564 161164571 161164586 165164588 153164590 153164610 161164688 161164701 162164702 162164703 162164707 170164708 170164709 170164730 164165195 161165218 16165250 131165305 152165306 152166508 26167008 145167063 20167064 20167525 134167652 134167695 131168055 145168136 150168381 26169558 26170009 131, (136, 137)170615 134170769 134170920 34171080 30171099 26171862 34172958 26173200 134173208 134173238 132, (136, 137)173239 132173240 132, (136, 137)173248 130, 134173249 130, 134174888 26174926 26174934 27, (29, 196)174942 27, 196174950 27, 196174969 27, 196174977 27, 196174985 27, 196176740 20176953 136177372 30177380 30177399 30177402 30177410 30177429 30177437 30177445 30177453 34178599 30178802 13178883 12178905 12178983 12, (14, 183)179553 134179693 17179707 17179820 21179830 85228100 104228101 104228102 104228103 104228104 104228105 104228106 104228107 104228108 104228109 104228110 105228111 105228112 105228113 105228114 105228115 105228116 105228117 105228118 105228119 105228120 105228121 105228122 105228123 105228124 105228125 105228126 105228127 105228128 105230012 110, (112, 113)230013 110, (112, 113)230302 117230305 117230351 118230381 118230582 111, (112, 113)230583 111, (112, 113)230584 111, (112, 113)230667 111230885 114230895 114230971 114231632 120231633 120231636 120231638 120231639 120231641 120231642 120231643 120231644 120231647 119231650 120231652 119232034 109232042 109232298 109232689 109, 177232698 110, 178232699 176232700 109, 177232701 176232702107, 108, 110,111, 176235305 176235306 176235307 110, 111, 178236001 73, 149236004 73236105 147236107 147236108 147236269 63, 178236366 63, 178236370 63, 178236701 176236702 176236703 176236707 176236710 176237105 147237107 147237108 147240074 165240182 169240187 111240401 126240541 126240600 110, (112, 113)240835 124240845 124241205 176242001 105242002 105242757 161242763 164242764 164242765 161242811 123242850 161244105 71248150 107, 149248161 114248259 107248262 107248450 107248650 107248909 108248917 108249182 107, 108249344 107198

www.nuncbrand.com - Always with updated informationPages listed in parentheses containreferences to the Cat. No.Cat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page249570 151249662 151249720 176249935 151249940 151249943 155249944 155249945 155249946 155249947 155249949 155249950 155249952 151249964 126250002 174 ,175250003 174 ,175250005 174 ,175250050 177250105 107, 108250385 123, (122)250393 122250520 122250539 122250555 122250875 114251357 110, 115251586 127253287 127253601 169253607 169253614 169253623 174 ,175253624 174 ,175253988 127254399 127254410 127254437 127254925 126255500 116255983 174 ,175256510 145259684107, 108, 110,111, (60)260210 150260251 157, (158)260252 157, (158, 159)264705 162260836 145260844 145260860 145260887 145260895 145262146 150262160 161262162 150262260 161262360 161263339 174 ,175263991 126264122 174 ,175264262 96264263 96264300 96264335 96264340 163264360 163264408 96264416 96264430 163264431 111, (112, 113)264440 96264459 96264460 163264467 96264491 96264556 161264573 166264574 166264575 166264576 166264579 166264605 110, (112, 113)264611 174 ,175264612 174 ,175264616 174 ,175264622 175264623 174 ,175264626 175264675 166264704 162264706 162264710 170264711 170264712 170264728 123265196 161265202 161265203 161265300 153265301 152265302 152265643 110, 114267011 110, (112, 113)267060 125267061 125267062 125267245 154267334 154267342 154267350 154267369 154267385 154267407 154267459 167267460 167267461 167267462 167268152 150268200 150269080 110, 114269390 168269620 146269787 146270972 96276000 171276002 171276003 171276004 171276005 171276011 171276014 110, 111, 178277143 151278010 159278011 159278012 159278605 158, (175)278606 158, (175)278616 158278743 157, (159)278752 158, (159)330040 41330041 53330042 53330043 53330050 41330821 45330856 45333510 92, (91)333511 92334940 89334959 89335505 45336078 39, (38)336081 39, (38)336088 39, (38)336092 39, (38)336094 39336095 39, (38)336096 39, (38)336097 39, (38)337516 42, (183)337846 91339497 89339993 48340053 46340061 46340529 45340711 43, (48, 183)341173 98, (99)341378 98341440 98341483 45, (44)341661 98341866 80, 99342080 45342110 89342129 89342919 98343001 98343036 80, 99343141 98343850 48343869 48343923 98343958 49, (38, 43)344260 172344261 172344262 172344263 172344280 172344281 172Product Reference List199

Always with updated information - www.nuncbrand.comProduct Reference ListProduct Reference ListCat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page344282 172360585 93369643 53, (52) 374094 52430805 63344283 172361239 98369644 53374097 52431615 66344380 172361247 39, (38) 369647 46374099 52434797 63344381 172361883 92, (91) 369658 53374100 52436006 70, 146344382 172362308 91369738 92, 93, (91) 374110 52436007 70, 148344383 172362694 90369740 92, (91) 374120 52436008 70, 148345586 99362695 90373514 40374130 52436009 160345608 99362696 90373530 40374179 89436011 160347155 47, 99, (43) 362697 90373660 89374187 45, (44) 436012 160347325 89362707 91373687 89374357 45436013 70347597 42362820 92, (91) 374009 53375299 43, (48) 436014 72, 146347627 42363282 93374011 53375353 42436015 72, 148347643 42363401 39, (38, 183) 374017 52375418 42, (183) 436016 72, 148347651 95363436 39374018 52375868 48436017 160347708 91363452 39, (38, 183) 374019 52375884 48436018 160347759 91364211 93374021 52375906 48436019 160347783 95364238 93374025 52375922 48436020 72347791 95364246 93374026 52375930 48436022 72347856 91364882 93374027 52376589 47, (38, 42, 95) 436023 70347880 91365048 93374028 53376813 89436024 74, 146347885 91366036 88374066 52377224 39436026 74, 148347910 95366052 88374067 52377267 39436027 74, 148347929 95366060 88374070 52377272 40436028 160348097 91366079 88374074 52377274 40436029 160348224 91366524 39, (38, 183) 374075 52377585 89436031 160348801 80, 99 366656 39, (38, 183) 374078 52378220 46436032 75, 146349638 98, (99) 367000 44374079 52378247 46436033 75, 148351934 39, (38) 367001 173374080 52, (53) 378441 48436034 75, 148354755 48367002 173374081 52, (53) 378468 48436036 160354879 48367005 44374082 52378476 48436037 160354968 48367006 44374083 52378484 48436038 160355018 48367007 44374084 52378492 48436110 69, 147355077 48367014 53374085 52378506 48436111 69, 147355158 48367997 39, (38) 374086 52379146 39, (38, 183) 437111 69, 147355501 44368632 39, (38, 183) 374087 52379189 39, (38, 183) 437112 69, 147355581 99369639 46374088 52430082 63437591 69356790 93369640 53374089 52430341 61, 149 437702 69360577 93369641 53374090 52430414 66437796 69, 149200

www.nuncbrand.com - Always with updated informationPages listed in parentheses containreferences to the Cat. No.Cat. No. Page Cat. No. Page Cat. No. Page Cat. No. Page437842 69, 149 456529 61, 145 473245 78, (122) 561077 94437869 69, 149 456537 61, 145 473539 66, 67 561301 97437877 69460348 62473709 63569746 94437915 69460372 161473717 631060-05 138437958 69, 149 460435 161473768 66, 67 1060-20 138438733 77460440 161475078 63, 67 1060-50 138439225 77460518 161475086 63, 67 1060-52 138439454 61, 146 460984 61, 145 475094 61, 146 1060-85 138441254 63463200 59475434 61, 150 1760-20 138441653 63463201 59475477 811860-22 138442404 61, 146 464394 63475515 692160-05 138442587 156464718 161475523 692160-20 138443990 80465219 78475574 793080-01 138444202 81465404 64476503 814260-22 138444474 81466966 63476619 785039-0048 173444865 63466982 80476635 785039-0072 173445101 63, 67 467120 63478042 71445497 78467140 63533669 100446140 61, 149 467320 61, 145 534479 47446442 65, 67 467340 61, 145 534592 47446469 65, 67 467466 63536080 97446470 65467679 63536323 97446471 59468608 80536455 97446473 59468667 63, 67 536617 97446477 65469078 63536668 97446490 65469264 62536684 97446612 61, 149 469329 62536757 97446639 65469914 62536889 97448143 174 ,175 469922 62537745 92448496 65469949 62538318 92, (91)448526 65469957 62540657 100448698 77470174 76540673 100448701 77470175 76549263 94449824 61, 150 470319 81550253 100452256 77470378 77550970 100453603 169472230 79551314 92453690 71472400 77554569 76455493 76472419 77560623 94Product Reference List201

Always with updated information - www.nuncbrand.comKeywordsKeywords96 Well Plates.....60, 61, 69, 70, 72-75, 145-15996 Cap Mats......................................................... 171384 Well Plates.......................................... 160-1681536 Well Plates.........................................169-170Cell Culture Range...............................................10Cell Factories...............................................131, 132Cell Factories, Accessories............................. 134Cell Factories, Active gassing....................... 132CryoTubes, External Thread..................... 42, 43CryoTubes, Internal Thread...................... 38, 39CryoTubes, Internal Thread, Barcoded....... 40CryoTubes, Scanner............................................ 53Cell Factories, Custom Capabilities............ 135Custom Barcoded CryoTubes......................... 411-Cell Stage Embryo Toxicity Testing.......... 84Cell Factories EasyFill....................................... 130Custom Coating, MicroWell Plates............. 1802D Coded CryoTubes.................................. 50, 52Cell Factory Hand Manipulator................... 136Custom Molding................................................ 1812D MicroHex....................................................... 139Cell Factory Instructions................................ 133Custom Services, Introduction.................... 179AAluminum CryoRack, 67 cm............................ 44Aluminum Sealing Tape..................................176Amplification Cooler.........................................116Amplification Plates.................................110, 111Amplification Tapes...............107, 108, 110, 111Amplification Tubes and Strips....................114Amplification Workstation.............................115Array Slides.........................................117, 118, 120ART/IVF Introduction......................................... 83Automatic Cell Factory Manipulator......... 136Automation Friendly CryoTubes................... 50BBank-It Accessories............................................. 53Bank-It Vials.................................................... 50, 52Barcode Scanner................................................. 41Barcoded CryoTubes................................... 40, 41Barcoded Flask................................................... 183Barcoded Products..................................182, 183Benchtop Rack Holder...................................... 53Bio-Assay Dish.................................................... 124BreakApart Modules................................... 66, 67Bulletins................................................................ 195CCap Mats............................................................... 171Caps for Flasks..........................................11, 13, 14Caps for Non-treated Flasks............................13Caps for CryoTubes, Colored.......................... 39Cardboard Storage Boxes................................ 46CC² Chamber Slides............................................ 31CE Marked Dishes for IVF................................. 84Cell Culture Inserts...................................... 22, 25Cell Factory, Automatic Manipulator........ 136Cell Factory, CO₂ Incubator........................... 137Cell Scrapers...........................................................17Centrifuge Tubes, 10/11 ml............................. 91Centrifuge Tubes, 15 ml.................................... 88Centrifuge Tubes, 50/200 ml.......................... 89Centrifuge Tubes, EZ Flip................................. 90Chamber Slides............................................. 30, 31Chambered Cover Glass................................... 32Chemical Resistance Chart............................ 188Chemical Resistance Thermanox............... 194Collagen I Multidish........................................... 20Contact Dish 67 X 15........................................ 126Containers, Sputum........................................... 94Containers.............................................................. 97Containers, Snap Cap........................................ 97Cooler Amplification.........................................116CovaLink NH Modules....................................... 71Coverglass............................................................. 30Coverglass, SlideFlask........................................ 34CoverSlips............................................................... 27Cryobank Accessories....................................... 53Cryobank Rack..................................................... 53Cryobank Vials............................................... 50, 52Cryo Color Coders............................................... 48CryoFlex.................................................................. 49CryoPen................................................................... 48CryoQuot................................................................ 40CryoRacks........................................................ 44, 47CryoStore Boxes................................................... 45CryoStore Canes.................................................. 48CryoStore Tabs..................................................... 48CryoTube Selection Tool.................................. 53CryoTubes, Accessories..................................... 48CryoTubes, Bank-It....................................... 50, 52CryoTubes, Barcoded.................................. 40, 41CryoTubes, Cryobank Vials....................... 50, 52Customized Products...................................... 181DDIAPOPS Technique......................................... 106Dishes, Cell Culture............................................. 26Dishes, IVF.............................................................. 84Dishes, Rectangular......................................... 125Disposable Tubes......................................... 98, 99DNA Plasmid Prep Kit...................................... 161EEasyFill Cell Factories....................................... 130EasyFlasks................................................................11Embryo Toxicity Testing................................... 84EZ Flip Tubes......................................................... 90FFilter Cap, Non-treated Flasks.........................13Flask on Slide........................................................ 34Flaskette Glass...................................................... 34Flasks Cell Culture.......................................... 11-15Flasks Non-treated..............................................13FlipCap Tubes....................................................... 90Fluoronunc/LuminuncModules/Plates............................................. 68, 69Frame for BreakApart C8................................. 66Frame for Immuno Modules........................... 62Frame for Lockwell............................................. 64Frame for NucleoLink...................................... 107Fritted DeepWell Plates.................................. 161GGassed Cell Factories....................................... 132Geometry, Well and Tube.....................186, 187Glass Fiber Filter Plate..................................... 161202

www.nuncbrand.com - Always with updated informationGlass Slides, MicroArray.................................. 120HHandheld Electric Screwdriver forCryoTubes.............................................................. 53Hand Manipulator for CF40.......................... 136IImmobilizer96 ImmobilizerAmino Modules/Plates......................... 70, 14896 ImmobilizerStreptavidin Modules/Plates..............72, 14896 ImmobilizerNickel-Chelate Plates............................ 74, 14896 Immobilizer Glutathione Plates... 75, 148384 Immobilizer Plates,PS White, Black & Clear.......................160, 161Immuno 96 MicroWell Plates.......................... 61Immuno Assay - Do’s & Don’ts....................... 57Immuno Assay Products, About................... 58Immuno BreakApart Modules....................... 66Immuno LockWellFluoronunc/Luminunc Modules................... 59Immuno LockWell Modules..................... 64, 65Immuno Modules................................. 59, 62-66Immuno Modules, Accessories...................... 63Immuno Modules, Frames.............................. 62Immuno Sticks...................................................... 79Immuno Surfaces......................................... 56, 57Immuno TSP.......................................................... 78Immuno Tubes.............................................. 80, 81Immuno Washers................................................ 76Incubator, CO₂ for Cell Factories................. 137In Vitro Fertilization Products......................... 83In Vitro Roller Bottles........................................ 138Ink-Jet Barcodes......................................... 40, 182LLabel Barcoded CryoTubes............................. 41Lab-Tek Chamber Slide System.................... 30Lab-Tek Chambered Coverglass................... 32Lab-Tek Comparison Chart............................. 33Lab-Tek II - CC² Chamber Slide System....... 31Lab-Tek II Chamber Slide System................. 31Lab-Tek II Chambered Coverglass................ 32Labtop Cooler....................................................... 44Large Scale Cell Culture.................................. 129Lid Guide.............................................................. 175Lid Overview........................................................174Lids for 96 Plates........................................174, 175Lids for 384 Plates ....................................174, 175Lid, Ultra-Low Profile...............................174, 175Lid, Universal...............................................174, 175Lids..................................................................174, 175LifterSlip.................................................................119LiveCell Array Microscope Slides.................. 28Loops..................................................................... 127Low CrossTalk Plate.......................................... 169Low Profile Bio Assay Dish............................. 124LowBot Lid Nunc Version.......................174, 175LowBot Lid Universal...............................174, 175Luminunc/FluoronuncModules/Plates............................................. 68, 69MManual Screwdriver, CryoTubes.................... 53MAX-100 Accessories......................................... 45MAX-100 Cryostore Boxes............................... 45MegaMAX-100...................................................... 45MegaMAX-110, Accessories............................. 45MicroArray, Mseries LifterSlip.......................119MicroArray Slides, Glass.................................. 120MicroArray Slides, Maxisorp..........................117MicroArray Slides, Nucleolink.......................118MicroWell MiniTrays........................................... 77MicroWell Plate Copier.................................... 122MiniBoxes for CryoTubes................................. 47MiniSorp Tubes.................................................... 80MiniTrays, Cell Culture....................................... 29MiniTrays, Immuno............................................. 77Modules, Colored................................................ 67Modules, CovaLink NH...................................... 70Modules, Immobilizer................................ 70, 72Modules, Immuno................................ 59, 62-66Modules, MaxiSorp............................... 59, 62-69Modules, MediSorp..................................... 63, 65Modules, MultiSorp..................................... 63, 65Modules, PolySorp............................... 59, 62-69Modules, Polypropylene................................ 109Molding, Custom............................................... 183Mortar Kit.............................................................100Movettes...............................................................100Multidishes, Collagen I...................................... 20Multidishes, Poly-D-Lysine.............................. 20Multidishes for IVF, CE Marked...................... 84Multidishes, Non-treated................................. 21Multidishes, Nunclon......................................... 20NNeedles................................................................. 127New Products Overview................................ 2, 3Non-treated EasYFlask.......................................13Non-treated Flasks..............................................13Non-treated Multidishes.................................. 21NucleoLink..................................................106, 107NucleoLink Starterkit....................................... 107Nunclon Tube....................................................... 29Nunclon Surface, About......................................9OOmniTray Copier............................................... 122KeywordsInoculation Loops............................................. 127Microcarriers....................................................... 139OmniTray, MaxiSorp........................................... 78Inoculation Needle........................................... 127MicroHex.............................................................. 139OmniTrays............................................................ 123Inserts................................................................ 22-25MicroMAX-100...................................................... 45OmniTrays, Cell Culture.....................................16IVF Dishes............................................................... 84Microplate Rack................................................... 53OptiCell.............................................................18, 19IVF/ART Introduction......................................... 83Microplate Storage Racks...............................174Overview of Nunc Lids.....................................176Microscope Slides............................................... 27MicroWell Lid..............................................174, 175203

Always with updated information - www.nuncbrand.comKeywordsKeywordsPPall Biodyne Membrane................................. 123PCR CompatibilIty Chart.................................112PCR Plates............................................110, 111, 159PCR Tubes..............................................................114Petri Dishes.......................................................... 126Physical Properties........................................... 185Pipettes....................................................................17PlatesPlate Application Information..................... 143Plate Overview................................................... 14296 Well Plates.......................................145-159DeepWell Plates 1.0 ml, PS......................... 158Filter Plates 1.3 ml, PP.................................. 159Optical Bottom Plates, PS/Coverglass... 152C96 MicroWell Plates, PS............................. 149F96 Immobilizer Plates,PS Black & White............................................. 148F96 MicroWell Plates,PS Black & White............................................. 147F96 MicroWell Plates, PS Clear....61,145, 146U96 DeepWell Plates 1.3 & 2.0 ml, PP... 157U96 MicroWell Plates, PP Colored........... 154U96 MicroWell Plates, PS Clear................. 150V96 MicroWell Plates, PP............................. 156V96 MicroWell Plates, PP Colored........... 155V96 MicroWell Plates, PS Clear................. 151384 Well Plates................................... 160-168384 Plates, PP................................................... 166384 Plates, PS..........................................160, 161DeepWell Plates, PP...................................... 168Immobilizer Plates, PS.................................. 1601536 Well Plates..................................169-1701536 Well Plates, PS.............................. 169, 1701536 Well High Base Plates........................ 170Poly-D-Lysine Flasks...........................................11Poly-D-Lysine Multidish................................... 20Poly-D-Lysine Plates...............................145, 164Polypropylene Modules................................. 109Product Reference List............................197-201ProPur Kits...................................................104, 105Purification Kits.........................................104, 105RRack for CryoTubes............................................. 47Rack for Storage Vials........................................ 96Rack Holder, Benchtop..................................... 53Racks, Microplate Storage............................ 172Rectangular Dishes (1, 4, & 8)....................... 125Replication System........................................... 122Resins..................................................................... 185Roller Bottles, In Vitro....................................... 138SScanner and Software....................................... 53Scanner for Barcodes......................................... 41Screwdriver,Handheld Electric for CryoTubes.................. 53Screwdriver, Manual for CryoTubes............. 53Sealing Tapes.......................................................176Serological Pipettes............................................17SlideFlask 9 cm2................................................... 34Slides, Cell Culture.............................................. 27Snap Cap for Containers.................................. 97SoLo Flasks..............................................................14Storage Racks, Microplates........................... 172Storage Vial Rack................................................. 96Storage Vials.......................................................... 96Storage Vials Color Coders.............................. 96Stor-It Tubes.......................................................... 95TTapes............................................ 107-111, 176, 177Tech Notes..................................................195, 196Thermanox Coverslips...................................... 27Thermanox, Chemical Resistance.............. 196TopYield Accessories....................................... 108TopYield Starterkit............................................ 108TopYield Strips.................................................... 108Transport Containers.................................. 92-94TripleFlasks.............................................................15TSP............................................................................. 78Tube Selection Tool............................................ 53Tubes, Disposable........................................ 98, 99Tubes, Nunclon ................................................... 29Tubes, Stor-It ........................................................ 95TubeTray and Holder.........................................115Tubing Kit for Immuno Washers................... 76UUnfritted DeepWell Plate............................... 159Urine Sample Kit.................................................. 92Vial Boxes............................................................... 96WWashing Tray, TSP............................................... 78Well and Tube Geometry......................186, 187Well Cap Mats..................................................... 171Optical Bottom Plates, Polymer Base....164Spacer Plate......................................................... 107Optical Bottom Plates, PS/Coverglass... 165Spare Part Kit for Immuno Wash................... 76ShallowWell Plates, PS................................. 163Specimen Tubes.................................................. 92ShallowWell Standard Height Base, PP....167Sputum Container.............................................. 94ShallowWell Standard Height Base, PS....162Standard Containers...........................................97Starter Kit, NucleoLink.................................... 107Starter Kit, TopYield.......................................... 108Stoppers........................................................... 80, 99Storage Box........................................................... 53Storage Box, Cardboard................................... 46204

www.nuncbrand.com - Always with updated informationNotesAdditional Information205

Drug DiscoveryDiagnostics (OEM)Part of Thermo Fisher ScientificPlate GuideNEWChoosing the right plate surface can give you the best possible results, as wellas save time and money. Nunc Brand’s plate guide has been made to help youto select the best possible plate for your application. Simply enter your selectedcriteria and you will see the range of Nunc plates matching your request.Technical drawings of the plates are also available.ExampleFormat Any◦ 6 Well◦ 8 Well◦ 12 Well ◦◦ 16 Well ◦Polymer Any◦◦PP (Polypropylene)PS (Polystyrene)Sterile◦ 24 Well◦ 48 Well◦ 96 Well384 Well1536 Well Any◦ Yes◦ NoBottom Any◦ Conical◦ Flat◦ Round◦ C-shapedSurface Any◦ Non-treated◦ MultiSorp◦ MaxiSorp◦ MediSorp◦ PolySorp◦ NucleoLink◦ Glutathione◦ Nickel-Chelate◦ Streptavidin Covalent◦ Streptavidin Passive◦ Amino◦ Collagen I◦ CC2◦ Cell Culture◦ Silent Screen• Save time• Save money• Better resultsType Any◦ Solid◦ Strip◦ OBP (Optical Bottom Plate)Color Any◦ Clear◦ White◦ Black◦ Natural◦ Red◦ Blue◦ YellowCell Culture Cryogenics Immuno AssaysART/IVFART/IVFProducts forA sistedReproductiveTechnologiesStorage & HandlingProteomics/GenomicsNunc creates high quality labware for use in biotechnology, pharmaceutical andresearch laboratories, as well as in the production of vaccines and diagnostic kits.... And by the way! We also work in close cooperation with NalgeneThe Plate Guide is alsoaccessible via direct links:www.nuncplateguide.comwww.plateguide.com©2007 Thermo Fisher Scientific Inc. All Rights Reserved.Denmark · Tel +45 4631 2000 · info.nunc@thermofisher.com · www.nuncbrand.comGermany · Tel +49 611 18674-0 · info.nuncde@thermofisher.com · www.nunc.deUSA · Tel +1 800 625 4327 · nnitech@thermofisher.com · www.nalgenunc.comJapan · Tel +81 3381 63355 · info@nalgenunc.co.jp · www.nalgenunc.co.jpwww.nuncbrand.cominfo.nunc@thermofisher.comNo. 32065 Ver.4.0 - N90000 - YNI - 04/2011