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www.nuncbrand.com - Always with updated informationTripleFlasks Nunclon∆ Surface• Three parallel growth surfaces providea total culture area of 500 cm²• External dimensions of a 175 cm²standard flask• Ideal for scale-up• Extra caps in every carton• NunclonΔ certified• Barcoded with large Code 128Cell CultureLiterature:Bulletin Nos: 2, 3, 5Tech Note Nos: 2, 3, 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksAccessories:Caps for Flasks NunclonΔ Page 14Serological Pipettes Page 172aNunclon51BOTTOMTOP2TOPBOTTOM3TOP4NunclonBOTTOM1. Prepare homogeneous cell suspension. Pourinto the TripleFlask, tilting flask slightlyto avoid foam or bubbles. Recommendedworking volume is 100-200 ml2. Leave the flask in the upright posi tion for ashort time to allow equilibration of liquidin each compartment2a. The flask may be canted momentarilyaround the connecting channel cornerfor facilitating the equilibra tion of smallvolumes3. Quickly but gently place the flask in theincubation position4. The liquid is equally distributed over thethree growth surfaces5. The flask is emptied in the same way as aconventional flask. To harvest cells add10-15 ml TrypsinNunc TripleFlasks NunclonΔPolystyrene. SterileCat. No. 132867 132913 132920Barcode - - +Culture area, cm² 500 500 500Neck style Straight Straight StraightClosure Vent/close Filter FilterVent/close CapFilter CapCap material HDPE HDPE HDPESuggested working vol., ml 200 200 200Units per pack/case 4/32 4/32 4/32HDPE = High Density Polyethylene15