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www.nuncbrand.com - Always with updated informationApplication InformationSurface area, liquid height and liquid volume dataIn recent years many new plate formatshave been introduced.Dense plate formats, such as 384 and1536 MicroWell plates, are being usedto decrease assay volume and handlingtime. However, those formats aredifficult to handle in the initial manualassay development. In assay development,tests are often made in simplerformats, such as 96 MicroWell plates.After development, the assays aretransferred to the denser formats.Most of the time the transformationis very straightforward, but the resultscan deviate from the originaldevelopment results.This may be due to the difference insurface to volume ratio as the kineticsof a solid phase assay can change inthe smaller wells. The liquid height isa relevant factor for OD, but may alsoplay a role in homogeneous assays.We hope to help you in the shiftbetween different plate formats withthe information on the followingpages. If you have additional questionsor need more information, please donot hesitate to contact us.PlatesLiquid height (H) vs. liquid volume (V)H mm121086F96 MicroWell plate.Pinchbar and OpticalBottom Plate designF96 MicroWell plate.Straight flange designU96 MicroWell plateC96 MicroWell plate4200 50 100 150 200 250 300 350 400V µl12Liquid height (H) vs. liquid volume (V)11109F96 MicroWell plate384 MicroWell plate384 ShallowWell plate1536 MicroWell plate87H mm65432100 25 50 75 100 125V µl143