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Always with updated information - www.nuncbrand.comTopYield StripsProteomics & Genomics• Designed for liquid phase PCR orImmuno-PCR• Made of polycarbonate• Excellent protein binding• Excellent heat transmission• Comparable with 0.2 ml PCR tube• Compatible with most 0.2 ml thermalcycler formats• Sealable with heat-stable Tape 8Literature:Tech Note Nos: 18, 19 Vol. 5., 35See Pages 195-196 for full reference list with titles and linksbiotinylateddetectionantibodyartigencaptureantibodyMicroWellPlatestreptavidin - DNAoligomerImmuno-PCRdouble biotinylatedand hapten-labeledPCR-productantibodyenzymeconjugate*STV-coatedsurfacePCR-ELISA*) STV = StreptavidinImmuno-PCR detection of an antigen with subsequentquantitative analysis of product yields by PCR-ELISA.Capture antibodies are immobilized on MicroWellplates to bind the antigen selectively. Sequentialcoupling of a biotinylated detection antibody, streptavidin,and a biotinylated DNA marker assemblea signal generating immuno-complex. Signalamplification by PCR using a biotinylated primer anda digoxigenin-labeled nucleotide generates doublylabeled amplificate which can be quantified in aPCR-ELISA assay. The PCR pro ducts are immobilizedon streptavidin-coated plates and analyzed by antidigoxigeninIgG-alkaline phospatase conjugate witheither chromogenic or fluorogenic substrates.Nunc TopYield FrameCat. No. 249182DescriptionFrameColorRedExternal dimensions, mm 128 x 86Heat-stable to max. °C 90MaterialAcrylonitrile Butadiene StyreneUnits per pack/case 6/72Nunc TopYield StripsCat. No. 248909DescriptionTopYield StripsColorClearTotal volume, µl/well 330Suggested working 100volume, µl/wellTotal surface area, mm² 234MaterialPolycarbonateUnits per tray/pack/case 12/120/1440Nunc TopYield AccessoriesCat. No. 250105 ‡ 248917 ‡ 232702 259684*108DescriptionTape 8 andSpacer PlateTopYieldStarter Kit**AmplificationTape 96Spacer PlateExternal dimensions, mm 80 x 8.8 / 115 x 80 x 3 - 120 x 79 115 x 80 x 3Material Polyester - Polyolefin SiliconeAdhesive Silicone Pressure sensitive acrylate -Units per pack/carton/case Tape 8: 60/480/5760 Spacer 1/12** 100/100 1/1Plate: 1/12* Reusable and autoclavable** Per kit: 48 TopYield Strips, 1 Frame; 60 Tape 8, 1 Spacer Plate and Application Literature‡ Not available in Americas

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