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Always with updated information - www.nuncbrand.comLab-Tek Chambered CoverglassCell Culture• Excellent for confocal image analysis• Optimal for high power invertedmicroscopic viewing• Chambered coverglass• Medium chamber is not removable• CE markedLab-TekChambered CoverglassCE marked. Sterile.1.0 Borosilicate coverglass.Cat. No. 155361 155380 155383 155411Number of wells 1 2 4 8Medium chamber Polystyrene Polystyrene Polystyrene PolystyreneSlide material Glass Glass Glass GlassSuggested working vol., ml 2.5 - 4.5 1.2 - 2.0 0.5 - 0.9 0.2 - 0.4Culture area, cm²/well 9.4 4.2 1.8 0.8Units per pack/carton/case 8/16/96 8/16/96 8/16/96 8/16/96Literature:Bulletin No: 13Tech Note Nos: 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and linksLab-Tek II Chambered Coverglass• Excellent for confocal image analysis• Optimal for high power invertedmicroscopic viewing• Chambered coverglass• Medium chamber is not removable• CE markedLab-Tek IIChambered CoverglassCE marked. Sterile.1.5 Borosilicate coverglass.Cat. No. 155360 155379 155382 155409Number of wells 1 2 4 8Medium chamber Polystyrene Polystyrene Polystyrene PolystyreneSuggested working vol., ml 2.0 - 4.5 1.0 - 2.0 0.5 - 1.0 0.2 - 0.5Culture area, cm²/well 8.6 4.0 1.7 0.7Units per tray/pack/case 8/16/96 8/16/96 8/16/96 8/16/96Literature:Bulletin No: 13Tech Note Nos: 25, Vol. 4: 34, 35, 36, 37See Pages 195-196 for full reference list with titles and links32
www.nuncbrand.com - Always with updated informationComparison of Lab-Tek and Lab-Tek IICell CultureNunc Chamber Slide SystemFeature Lab-Tek Product Lab-Tek II ProductConfigurations 1, 2, 4, 8, 16 well, flask style 1, 2, 4, 8 wellMedium chamber material Proprietary cell culture grade polystyrene Proprietary cell culture grade polystyreneSlide materialsCoverSealantSoda Lime glassPermanox plasticPolystyrene plasticClear polystyrene, no handle, facilitatesmicroscopic viewingNon-toxic medical grade siliconeRemains on slide after detachmentSoda Lime glassClear polystyrene with top handlefor aseptic manipulationsBiocompatible acrylic adhesive - Remains withmedia chamber after detachmentReagent “dam” Created by removable silicone gasket Created by hydrophobic borderComponent separation No separation tools required Requires separation toolsGrowth surfaceFor adherent cell culture:Glass slides - Six stage high purity water washPermanox Plastic - Cell culture treatedPolystyrene - Cell culture treatedBiological testing BHK-21, HEp-2 cells BHK-21, HEp-2 cellsLeak tested Yes YesSlide background fluorescenceGlass: No endogenous fluorescencePermanox: Minimal fluorescencePolystyrene: Autofluorescence < 485 nmCoverslip compatibility Yes, following gasket removable YesFor adherent cell culture:RS treated glass slides - Proprietary washCC² - chemically modified growth surfaceGlass: No endogenous fluorescenceWriting surface “Sand blasted” frosted end Superfrost with printed endChambered Coverglass SystemFeature Lab-Tek Product Lab-Tek II ProductConfigurations 1, 2, 4, 8 well 1, 2, 4, 8 wellMedium chamber materialCoverglass materialsCoverProprietary cell culture grade polystyreneNon-removableBorosilicate glassNo. 1 thickness (0.13 - 0.17 mm)Clear polystyrene, no handle facilitatesstackingProprietary cell culture grade polystyrene -Non-removableBorosilicate glassNo. 1.5 thickness (0.16 - 0.19 mm)Clear polystyrene with handle foraseptic manipulationsSealant Non-toxic medical grade silicone Biocompatible acrylic adhesiveGrowth surface For adherent cell culture For adherent cell cultureBiological testing BHK-21, HEp-2 cells BHK-21, HEp-2 cellsLeak tested Yes YesSlide background fluorescence Glass·: No endogenous fluorescence Glass: No endogenous fluorescenceWriting surface None NonePackaging 8 tray, 16 pack, 96 case 8 tray, 16 pack, 96 caseSuperFrost is an Erie Scientific registered trademark.33
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