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www.nuncbrand.com - Always with updated information96 Filter Plates 1.3 mlFlow diagram for plasmid DNA purificationProcedure:Figure 1.Procedure onhow to useNunc FilterPlates for costeffective DNApurification.Grow E. Coli cells in a sterile 2 ml DeepWell Plate(Cat. No. 278743). (overnight)Lyse bacteria and neutralize lysate. (15 min.)Clarify plasmid DNA with Fritted Filter Plate(Cat. No. 278011). (1 min.)Bind plasmid DNA to Glass Fiber Filter Plate(Cat. No. 278010). (3 min.)Wash and dry membrane. (8 min.)Elute pure plasmid DNA with TE into 1 ml receptionDeepWell Plate (Cat. No. 260252). (12 min.)• For efficient purification of highquality plasmid DNA or PCR products• Designed specifically for highthroughput sample processing• Conforms to standard 96 well footprint• Made of polypropylene offeringchemical resistance and mechanicalstrength• Suitable for vacuum filtration, centrifugationor positive pressure techniques• Polyethylene terapthalate (PET) frit infritted plate has 20 µm pore size• Glass fiber plate has PET frit and 2 layersof glass fiber membrane forbinding of DNA• Shared wall configuration givesreduced plate height, wider wellsfor faster processing, higher yieldsand less clogging• Purified DNA is suitable fordownstream applications such assequen cing and restriction digestionLiterature:Bulletin Nos: 39, 40See Pages 195-196 for full reference list with titles and linksAccessories:96 DeepWell Plates Page 157-158Sealing Tapes Page 176U96 MicroWell Plates 500 μl Page 154V96 MicroWell Plates 450 μl Page 155PlatesNunc 1.3 ml Filter PlatesCat. No. 278010 278011 278012DescriptionGlass Fiber 96 Well FilterFritted 96 DeepWell PlateUnfritted 96 DeepWell Plate(Binding) PlateTotal volume, ml 1.3 1.3 1.3Recommended1.0 1.0 1.0working volume:Frit Size, µm 20* 20 -Material Polypropylene Polypropylene PolypropyleneSterile - - -Units per pack/case 5/50 5/50 5/50* Has 2 layers of glass fiber membrane on top of the PET frit159