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www.nuncbrand.com - Always with updated informationWhat is the difference between the Anopore and the Polycarbonatemembrane used in the cell culture inserts?Both the membranes are speciallytreated to provide a surface that isexcellent for cell attachment andgrowth. The Anopore membraneis a rigid inorganic membrane thatis highly transparent when wetand highly porous (40-50% surfaceporosity). This membrane providesmaximum clarity for microscopy, isnon-autofluorescent, and highly resistantto most solvents. Some typicalapplications are epithelial polarizationstudies, transport studies, toxicitytesting and electron microscopy. Anapplications manual is available. SeeTechnical articles index to order.The Polycarbonate membrane does nothave the transparent characteristicsof the Anopore membrane. Due tothe larger pore sizes available with thepolycarbonate membrane, it is particularlysuitable for chemotaxis studies.These membranes are easily removedand are ideal for sectioning for transmissionelectron microscopy using aglass knife.Cell CultureNunc Cell Culture InsertsPolycarbonate membrane.SterileCat. No. 137052 137060 137370 137435 137443 137508Pore size, µm 0.4 0.4 3.0 3.0 8.0 8.0Diameter size, mm 10 25 10 25 10 25For use in Multidish 24 Multidish 6 Multidish 24 Multidish 6 Multidish 24 Multidish 6Pore density, pores/cm² 1.5 x 10⁸ 1.5 x 10⁸ 3 x 10⁶ 3 x 10⁶ 10⁵ 10⁵Porosity % 18 18 14 14 5 5Film thickness, micron 20 20 17 17 12 12Height above base of well, mm 1 1 1 1 1 1Culture area, cm² 0.5 4.2 0.5 4.2 0.5 4.2Suggested working volume, ml 0.5 1.75 0.5 1.75 0.5 1.75Units per pack/case 6/48 6/48 6/48 6/48 6/48 6/48All Nunc Cell Culture Inserts: Suggested working volume, ml = in addition to normal well working volume.25

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