BIOGRAPHY OF A RIVER

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II.ActivityA. Have students write a hypothesis about what they think the results of the fecalcoliform testing will be and write a brief paragraph about why they made specificpredictions.(If using a coliform testing kit, follow the directions for procedure. Otherwise follow thedirections below.)B. Filter 100ml of each sample using a small filter. Clean all equipment before startingand between each sample.C. Use tweezers to remove the filter paper and place in a sterile petri dish.D. Break open an ampule of the m-ColiBlue 24 broth and pour over the filter paper.Label each dish.oE. Incubate at 44.5 C for 24 hours.F. Examine for growth at the end of 24 hours. Total coliform counts can be attainedby counting both the red colonies and the blue colonies. For E. Coli counts, countonly the blue colonies.G. Record data on Student Sheet.III. Follow-upA. Have students write a statement accepting or rejecting their hypotheses. Explainwhy the test turned out like it did.B. Have students list some possible sources of coliform contamination in the samplesthat were positive.C. Discuss how these contaminants affect the cost of purifying drinking water. Askstudents for ideas on how to prevent contamination.IV. Extensions3-70
A. Students could research the different pathogenic microorganisms found in water.Each student could report on one disease.B. Discuss nonpoint source pollution and problems this can cause.RESOURCESHigh school biology book or high school environmental science book.Jacobson, Cliff, Water, Water Everywhere, (Student Reading Unit About Water Quality), HachCompany, Loveland, CO, Catalog Number 21975-00. 1-800-227-HACH.Water Analysis Kit, page F-69, Water Testing Fact Sheet3-71
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B. Read descriptions of rivers in l
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CATCH ME IF YOU CAN --9-12 (TWO WAY
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III.Depending on the units used for
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. Be sure that the water supply pai
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3-15Student Sheet
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1. the littoral zone: shallow water
Page 19 and 20: profundal zone: a zone in a lake ex
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Page 27 and 28: algal bloom: a sudden increase in t
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Page 31 and 32: manufacturing and for removing the
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Page 45 and 46: so that the forests can continue to
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Page 53 and 54: was incubated naturally at atmosphe
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Page 59 and 60: III.Follow-upA. To explain further
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Page 63 and 64: PROCEDUREI. Setting the stageA. Stu
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BIOGRAPHY OF A RIVER

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