Case Reportpsychological well-being. It is worth remembering thatwe cannot give the patient a living prosthesis of realtissues. The day prosthesis behaves and looks like a realtissue, will be the day when perfection is achieved.References1.2.3.4.The glossary of prosthodontic terms. J Prosthet Dent2005;94(1):10-92.Miglani DC, Drane JB. Maxillofacial prosthesis and itsrole as a healing art. J Prosthet Dent 1959; 9(1):159-68.Huber H, Studer SP. Materials and techniques inmaxillofacial prosthodontic rehabilitation. OralMaxillofac Surg Clin North Am 2002;14(1):73-93.Kanter JC. The use of RTV silicones in maxillofacialprosthetics. J Prosthet Dent 1970;24(6):646-53.5.6.7.8.9.Javid N. The use of magnets in a maxillofacial prosthesis.J Prosthet Dent 1971;25(3):334-41.Pekkan G, Tuna SH, Oghan F. Extraoral prosthesesusing extraoral implants. Int J Oral Maxillofac Surg2011;40(4):378-83.Manurangsee P, Isariyawut C, Chatuthong V,Mekraksawanit S. Osseointegrated finger prosthesis: Analternative method for finger reconstruction. J HandSurg Am 2000;25(1):86-92.Kini AY, Byakod PP, Angadi GS, Pai U, Bhandari AJ.Comprehensive prosthetic rehabilitation of a patient withpartial finger amputations using silicone biomaterial: Atechnical note. Prosthet Orthot Int 2010;34(4):488-94.Thomas KF. Prosthetic Rehabilitation. Quintessence,London 1994:127-8.410Indian Journal of Multidisciplinary Dentistry, Vol. 2, <strong>Issue</strong> 1, <strong>Nov</strong>ember 2011 to <strong>Jan</strong>uary 2012
Bordetella avium and Bacillus megaterium inEndodontic Infectioncase reportArunajatesan Subbiya*, Krishnan Mahalakshmi**AbstractMany microbiological studies on endodontic infections indicate a complex polymicrobial community. Different identificationmethods used have revealed a diverse microflora in the endodontic niche. Recently 16S rRNA/DNA gene sequencing methodof identification is widely employed in dentistry which has discovered novel pathogens that may be uncultivable or possiblyslow growing and difficult to identify. In the present study, nested polymerase chain reaction (PCR) was performed with theendodontic samples of the two patients with diffuse swelling and pain near the region of tooth with prosthetic crown as thesetwo samples were culture negative. 16S rRNA universal eubacterial primers were used for rapid identification. Unusually, theorganisms identified in both the cases were of single etiology. Bordetella avium was identified in the endodontic sample ofa tooth with prosthetic crown in a 56-year-old woman and Bacillus megaterium in a 65-year-sold man. The occurrence ofB. avium and B. megaterium on the teeth may be correlated to their ability to secrete collagenase. Direct screening of theclinical samples by molecular approach has identified unusual human bacterial pathogens in tooth with prosthetic crown. Inaddition, the results of this study also reveal that endodontic infection need not be polymicrobial all the time. Screening ofbacterial pathogens in the endodontic samples may help in treatment planning and treatment evaluation.Key words: Bordetella avium, Bacillus megaterium, endodontic infection, prosthetic crown, 16S rDNA sequencingBordetella avium is thought to be strictly anavian pathogen. It is the etiologic agent ofbordetellosis, a highly contagious upperrespiratory disease of young poultry. Its prevalenceamong domesticated turkeys is well-documented. 1B. avium is a gram-negative, nonfermentable, aerobicand motile bacterium that colonizes the trachea ofchicken, turkeys, cockatiels, ostriches and many otheravian species. It is an opportunistic pathogen inchicken. 2 Human infections by B. avium is rare. Bacillusmegaterium, a gram-positive, rod-shaped endosporeformingbacteria is a common soil saprophyte. It isconsidered aerobic, but, it is also capable of growingunder anaerobic conditions when indispensable.It finds wider applications in the environmentaland industrial needs. It can also survive in extremeconditions such as desert environments due to thespores it forms. The data regarding its association with*Professor, Dept. of Conservative Dentistry and Endodontics**Associate Professor, Dept. of MicrobiologySree Balaji Dental College and Hospital, ChennaiAddress for correspondenceDr Krishnan MahalakshmiAssociate ProfessorDept. of MicrobiologySree Balaji Dental College and Hospital, Velachery-Tambaram RoadChennai - 600 100, Tamil NaduE-mail: kmag_1985@yahoo.co.indental infection in humans is lacking. The presentstudy reports of endodontic infection caused byB. avium and B. megaterium in two different patient’steeth with prosthetic crown. In addition the presentstudy shows an unusual etiology as against usualpolymicrobial nature of endodontic infection reportedtill date. To the best of our knowledge this may be thefirst reported case in the tooth with prosthetic crown.Case Description and ResultsCase Report 1A 56-year-old Indian woman visited a private dentalhealth centre in Chennai, with the chief complaint ofdiffused swelling and pain in the periapical region ofright upper canine. Patient experienced a throbbingpain for almost a week. On intraoral examination, theconcerned tooth had a PFM crown. The tooth wasserving as an abutment for a bridge. This bridge hadbeen in place for the past four years and 6 months.The patient had history of generalized periodontitisand extraction of posterior teeth, but gave no historyof caries. The patient is nonvegetarian by food habit.The intraoral periapical (IOPA) radiograph showed aperiapical radiolucency of 2-3 mm diameter (Fig. 1).A root canal treatment was planned and access openingIndian Journal of Multidisciplinary Dentistry, Vol. 2, <strong>Issue</strong> 1, <strong>Nov</strong>ember 2011 to <strong>Jan</strong>uary 2012411