Faculty of Pharmacy - Mahidol University

290 Faculty of Pharmacy
ergosterol peroxide detected in mycosed and mummified cadavers
were very low (0.011 and 0.386 g/larva) less then levels that either
inhibited insect cell proliferation or caused insecticidal activity.
(World Journal of Microbiology and BiotechnologyVolume 24, Issue
12, December 2008, Pages 2909-2917.) (This research was supported
by the Royal Golden Jubilee Ph.D. Scholarship (PHD/0287/2545),
the Thailand Research Fund and Mahidol University.)
STUDY ON CYTOTOXICITY AND NUCLEOTIDE
SEQUENCES OF ENTEROTOXIN FM OF
BACILLUS CEREUS ISOLATED FROM VARIOUS
FOOD SOURCES. (NO. 786)
Boonchai, N. 1 , Asano, S.-I. 2 , Bando, H.2, Wiwat, C. 1,3
1 Department of Microbiology, Faculty of Pharmacy, Mahidol
University, Bangkok, Thailand; 2 Faculty of Agriculture,
Hokkaido University, Sapporo, Hokkaido, Japan; 3 Department
of Microbiology, Faculty of Pharmacy, Mahidol University, 447
Sri-Ayudhya Rd, Rachatwewee, Bangkok 10400, Thailand.
Key words : Bacillus cereus; Cytotoxicity; Enterotoxin FM; Food
poisoning; Sequencing
Objective: To determine the cytotoxicity of the crude
culture broth containing enterotoxins of B. cereus and investigate
the nucleotide sequences of ent FM among various isolates B. cereus.
Material and Method: The 1.2 kb fragment of ent FM of B. cereus
was amplified, cloned, sequenced, and compared with published
sequences. The biological activity of crude culture broth containing
enterotoxins with and without monoclonal antibodies against HBL,
NHE, and EntFM enterotoxins was tested using Vero cells assay.
Results: A percentage homology of nucleotide sequences and deduced
amino acid sequences among test isolates and published strains were
90-99% and 88-99%, respectively. An assays for cytotoxic activity
revealed that seven and three of B. cereus isolates were positive for
NHE enterotoxin and EntFM enterotoxin, respectively. In addition,
the 4-repeating sequences “TCAAAC” of ent FM were found, which
may or may not be probably correlated with cytotoxicity of B. cereus.
Conclusion: The enterotoxin FM from B. cereus isolates is cytotoxic
and the degree of cytotoxicity depends on the bacterial strain.
(Journal of the Medical Association of Thailand Volume 91, Issue 9,
September 2008, Pages 1425-1432.)
ANTIBACTERIAL ACTIVITY OF THAI MEDICINAL
PLANTS AGAINST METHICILLIN-RESISTANT
STAPHYLOCOCCUS AUREUS (NO. 787)
Mullika Traidej Chomnawang 1 , Suvimol Surassmo 1 , Karn
Wongsariya 1 and Nuntavan Bunyapraphatsara 2
1 Department of Microbiology, Faculty of Pharmacy, Mahidol
University, Bangkok, Thailand; 2 Department of Pharmacognosy,
Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
Key words: Methicillin-resistant S. aureus; Medicinal plants;
Antibacterial activity; Bioautography; Garcinia mangostana; -
mangostin
Methicillin-resistant Staphylococcus aureus (MRSA) is a
major nosocomial pathogen which causes severe morbidity and
mortality worldwide. Seventeen Thai medicinal plants were
investigated for their activity against MRSA. Garcinia mangostana
was identified as the most potent plant, and its activity was traced to
the prenylated xanthone, -mangostin (MIC and MBC values of 1.95
and 3.91 g/ml, respectively).
(Fitoterapia Article in Press ) (This work was supported by the
Mahidol University and the Thailand Research Fund.)
3D-QSAR STUDIES ON CHROMONE DERIVATIVES
AS HIV-1 PROTEASE INHIBITORS : APPLICA-
TION OF MOLECULAR FIELD ANALYSIS (NO. 788)
Nunthanavanit, P. 1 , Anthony, N.G. 2 , Johnston, B.F. 2 , Mackay, S.P. 2 ,
Ungwitayatorn, J. 3,4
1 Faculty of Pharmacy, Srinakharinwirot University, Nakhon
Nayok, Thailand; 2 Strathclyde Institute for Pharmacy and
Biomedical Sciences, University of Strathclyde, Glasgow, United
Kingdom; 3 Faculty of Pharmacy, Mahidol University, Bangkok,
Thailand; 4 Faculty of Pharmacy, Mahidol University, 447 Sri-
Ayudhya Road, Bangkok 10400, Thailand.
Key words : 3D-QSAR; Alignment; Chromone derivatives; HIV-1
protease; Molecular field analysis (MFA)
Three-dimensional quantitative structure-activity
relationship (3D-QSAR) models were developed for chromone
derivatives against HIV-1 protease using molecular field analysis
(MFA) with genetic partial least square algorithms (G/PLS). Three
different alignment methods: field fit, pharmacophore-based, and
receptor-based were used to derive three MFA models. All models
produced good predictive ability with high cross-validated r2 (r2cv),
conventional r2, and predictive r2 (r 2pred) values. The receptorbased
MFA showed the best statistical results with r2cv = 0.789, r2 =
0.886, and r2pred = 0.995. The result obtained from the receptorbased
model was compared with the docking simulation of the most
active compound 21 in this chromone series to the binding pocket of
HIV-1 protease (PDB entry 1AJX). It was shown that the MFA model
related well with the binding structure of the complex and can provide
guidelines to design more potent HIV-1 protease inhibitors. 2008
Wiley-VCH Verlag GmbH & Co. KGaA.
(Archiv der Pharmazie Volume 341, Issue 6, June 2008, Pages 357-
364) (This study was granted by Faculty of Graduate Studies,
Mahidol University.)
3D-QSAR INVESTIGATION OF SYNTHETIC
ANTIOXIDANT CHROMONE DERIVATIVES BY
MOLECULAR FIELD ANALYSIS (NO. 789)
Samee, W. 1 , Nunthanavanit, P. 2 , Ungwitayatorn, J. 2
1 Department of Pharmaceutical Chemistry and Pharmacognosy,
Faculty of Pharmacy, Srinakharinwirot University, Nakhon
Nayok, 26120, Thailand; 2 Department of Pharmaceutical
Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok,
10400, Thailand