Structural and Thermodynamic Characterization of T4 Lysozyme ...

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36. Peng, Q. and Li, H. (2008) Atomic force microscopy reveals parallel mechanical unfoldingpathways of T4 lysozyme: evidence for a kinetic partitioning mechanism, Proc. Natl. Acad. Sci.U.S.A. 105, 1885-1890.37. Elwell, M. L. and Schellman, J. A. (1977) Stability of phage T4 lysozymes. I. Native properties andthermal stability of wild type and two mutant lysozymes, Biochim. Biophys. Acta 494, 367-383.38. Kohn, J. E., Millett, I. S., Jacob, J., Zagrovic, B., Dillon, T. M., Cingel, N., Dothager, R. S., Seifert,S., Thiyagarajan, P., Sosnick, T. R., Hasan, M. Z., Pande, V. S., Ruczinski, I., Doniach, S. andPlaxco, K. W. (2004) Random-coil behavior and the dimensions of chemically unfolded proteins,Proc. Natl. Acad. Sci. U.S.A. 101, 12491-12496, [published erratum appears in (2004) Proc. Natl.Acad. Sci. U.S.A. 102, 14475-a].39. Pollack, L., Tate, M. W., Finnefrock, A. C., Kalidas, C., Trotter, S., Darnton, N. C., Lurio, L.,Austin R. H., Batt, C. A., Gruner, S. M., and Mochrie, S. G. J. (2001) Time resolved collapse of afolding protein observed with small angle x-ray scattering. Phys. Rev. Lett. 86, 4962-4965.40. Arai, M., Ito, K., Inobe, T., Nakao, M., Maki, K., Kamagata, K., Kihara, H., Amemiya, Y. andKuwajima, K. (2002) Fast compaction of alpha-lactalbumin during folding studied by stopped-flowX-ray scattering, J. Mol. Biol. 321, 121-132.41. Chen, L., Wildegger, G., Kiefhaber, T., Hodgson, K. O. and Doniach, S. (1998) Kinetics oflysozyme refolding: structural characterization of a non-specifically collapsed state using timeresolvedX-ray scattering, J. Mol. Biol. 276, 225-237.42. Tardieu, A., Le Verge, A., Malfois, M., Bonneté, F., Finet, S., Riès-Kautt, M., and Belloni, L.(1999) Proteins in solution: from x-ray scattering intensities to interaction potentials, J. Cryst.Growth 196, 193-203.32
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Page 1: Structural and Thermodynamic Charac
Page 5 and 6: Experimental ProceduresSample and B
Page 7: The next day, each 1.25 L culture w
Page 13 and 14: Fluorescence measurements were made
Page 15 and 16: I(q) decay in the intermediate q re
Page 17 and 18: Pressure-induced changes to the flu
Page 19 and 20: Unfolding at pH 3.0The volume chang
Page 21 and 22: additional solvent-binding sites. T
Page 23 and 24: BOTTOM QUARTILEIndia, Bangladesh, M
Page 25 and 26: high-pressure crystallography study
Page 27 and 28: esults support the growing view tha
Page 29 and 30: 10. Hummer, G., Garde, S., García,
Page 31: 27. Glatter, O. (1982) Data Treatme
Page 35 and 36: Table 1. Thermodynamic Quantities C
Page 37 and 38: Table 3. Denaturation volume change
Page 39 and 40: andom coil conformers (blue scatter
Page 41 and 42: (a) (b) (c)776655443328673119 1(d)
Page 43 and 44: 365360< λ > (nm)3553503453403350 5
Page 45 and 46: (a)P(r) (a.u.)10.80.60.40.2Iq 22015
Page 47 and 48: 365360(a)< λ > (nm)355350345340335
Page 49 and 50: Change in Water Occupancy432100 50
Page 51 and 52: Supporting Online MaterialSupportin
Page 53 and 54: Tris, 10 mM sodium EDTA, pH 8.0, pe
Page 55: 4. Muchmore, D. C., McIntosh, L. P.

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