Structural and Thermodynamic Characterization of T4 Lysozyme ...

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Radius <strong>of</strong> Gyration (Å)3632282420(a)160 50 100 150 200 250 3001(b)Normalized I(0)0.90.80.70.60.50 50 100 150 200 250 300Pressure (MPa)Figure 4.44
(a)P(r) (a.u.)10.80.60.40.2Iq 22015105100 0.1 0.22q (Å −1 )(b)I(q) (a.u.)4321Selection Freq.0.050.040.030.020.01010 30 50 70R g (Å)00 20 40 60 80 100 120 140r (Å)00 0.05 0.1 0.15 0.2q (Å −1 )(c)(d)Figure 5.Figure 1: default145
Page 1: Structural and Thermodynamic Charac
Page 5 and 6: Experimental ProceduresSample and B
Page 7: The next day, each 1.25 L culture w
Page 13 and 14: Fluorescence measurements were made
Page 15 and 16: I(q) decay in the intermediate q re
Page 17 and 18: Pressure-induced changes to the flu
Page 19 and 20: Unfolding at pH 3.0The volume chang
Page 21 and 22: additional solvent-binding sites. T
Page 23 and 24: BOTTOM QUARTILEIndia, Bangladesh, M
Page 25 and 26: high-pressure crystallography study
Page 27 and 28: esults support the growing view tha
Page 29 and 30: 10. Hummer, G., Garde, S., García,
Page 31 and 32: 27. Glatter, O. (1982) Data Treatme
Page 33 and 34: 43. Bernadó, P., Blanchard, L., Ti
Page 35 and 36: Table 1. Thermodynamic Quantities C
Page 37 and 38: Table 3. Denaturation volume change
Page 39 and 40: andom coil conformers (blue scatter
Page 41 and 42: (a) (b) (c)776655443328673119 1(d)
Page 43: 365360< λ > (nm)3553503453403350 5
Page 47 and 48: 365360(a)< λ > (nm)355350345340335
Page 49 and 50: Change in Water Occupancy432100 50
Page 51 and 52: Supporting Online MaterialSupportin
Page 53 and 54: Tris, 10 mM sodium EDTA, pH 8.0, pe
Page 55: 4. Muchmore, D. C., McIntosh, L. P.

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