Definiens Tissue Studio

DATA SHEET
Understanding Images
Definiens Tissue Studio TM
Definiens Oncology Suite
Definiens Tissue Studio TM is the most advanced digital pathology image
analysis solution for biomarker translational research.
Definiens Tissue Studio helps you to solve
biological questions
Definiens Tissue Studio is built on Definiens
Cognition Network Technology® and is the most advanced
digital pathology image analysis application
for translational biomarker research. It accurately
quantifies biomarkers, revealing the precise attributes
of sub-anatomical objects, cells and sub-cellular
objects, helping to solve your most challenging
biological questions.
Definiens Tissue Studio is easy to use
Every aspect of Definiens Tissue Studio has been
designed to support pathologists and research
scientists who seek further validation and understanding
of tissue-based biomarkers. It takes just
three simple steps to use.
1. Train the application to identify regions of interest
The application can be trained to identify regions
of interest automatically. Simply import sample
slides and highlight the regions of interest with the
”paintbrush“ tool, comparing up to four slides at once.
Then, simply click the ”Learn“ button.
The application uses your images to create a robust
algorithm for identifying the precise regions of interest
(ROI) which you seek. You can manually review the
regions of interest or they can form the basis of a fully
automated batch processing routine.
Figure 1 Definiens Tissue Studio supports the
simultaneous utilization of up to four images which
best represent variability in a given image set for
creation of a robust image analysis solution.
Definiens
Tissue Studio
Accurately measures
nuclear, membrane and
cytoplasmic biomarkers
Provides multiparametric
cell-by-cell quantitation
Enables simple, robust
detection of regions of
interest
Supports all standard IHC
tissue stains (blue, brown
and red), H&E and IF
Supports all tissue formats
including slides and TMAs
Scales to any size for batch
processing
Works out-of-the-box
Uses a simple interface
that requires no programming
skills

2
Definiens Tissue Studio
Figure 2 Definiens Composer Technology TM
in action: In order to classify the object as a tumor,
the user simply “paints” just a few representative ROIs with the paintbrush tool (green). This
process is then repeated across the other images, and for other ROIs (tissue, lymph node, etc.).
Figure 3 Definiens Tissue Studio TM control panel: The colored icons at the top are used to select
diferent paintbrush colors for sampling ROIs. After using the paintbrush tool, the user simply
clicks the “Learn” button to teach the system to properly identify ROIs. This entire process is
simple, and takes only minutes.
Figure 4 Close-up view of pancreas tissue after training the system how to recognize ROIs
using Definiens Composer Technology TM . Islets (both tumor and normal) are depicted in green,
tissue in blue, lymph nodes in dark blue and white space in white.

2. Create unique segmentation and classifcation for sub-cellular biomarker detection
Using the intuitive interface and pre-defned tools, you can create multiparametric segmentation and
classifcation with ease.
Definiens Tissue Studio conducts segmentation and classifcation on a cell-by-cell basis, giving you
unparalleled confdence and understanding of biomarkers that are related to disease progression,
toxicity, or therapeutic response.
Use the new, simplifed graphic user interface to train the application how to recognize the nuclear,
cytoplasmic, and membrane attributes of cells. Even with challenging images, the cell simulation
function will identify nuclear objects and then“grow”the cell out to establish its proper border.
Choose from a complete set of default multiparametric and morphological features you want to
measure – number of positive nuclei, number of negative nuclei, mean cell size, marker area,
staining intensity, and many more. Custom parameters are also available for export.
The solutions you develop with Definiens Tissue Studio will enable you to data mine your tissue
images and uncover underlying correlations related to clinical endpoints of interest.
Figure 5 In this example, a second Definiens Composer Technology step was used to further
classify islets into tumor (red) and normal (orange). ROIs (gray boxes) are then selected within
the tumor regions for subsequent cell-by-cell biomarker multiparametric quantifcation.
3. Run the dataset
Definiens Tissue Studio works with all standard image formats – Hamamatsu, Zeiss, Aperio, Applied
Imaging, TissueGnostics, Bacus – as well as generic formats like .tif and .jpeg. All you need to do
is import the slides and click ”run“.
It is that easy.
Definiens Tissue Studio can batch process locally or send larger jobs to servers. There is no limit to
the number of CPUs that can be added, ensuring that you can handle any project of any size.
Definiens Tissue Studio 3

4
Definiens Tissue Studio
Figure 6 Example of nucleus detection in pancreas tumor.
Definiens Tissue Studio is uniquely powerful
Definiens Tissue Studio is built on the powerful Definiens Cognition Network Technology®
which does not rely solely on traditional pixel-based, pattern recognition. Instead, it builds
an understanding of images iteratively, identifying objects through their characteristics, their
relationships to other objects and the context in which they occur.
This fundamental diference enables Definiens Tissue Studio to address highly heterogeneous
tissues, identify individual cells and the sub-cellular objects within them, and then
conduct extremely accurate cell-by-cell measurements.
Definiens Tissue Studio is part of Definiens Oncology Suite
Definiens Tissue Studio is part Definiens Oncology Suite which encompasses applications spanning
cellular assays, histopathology, and non-invasive medical imaging.
Defniens Tissue Studio incorporates Definiens Composer Technology
Definiens Composer Technology is a new easy to use machine learning approach that enables
the user to train the application to detect regions of interest using up to four images simultaneously.
The resulting solution can be applied to a complete set of images with a single click for unlimited
parallel batch processing.
Definiens Tissue Studio improves decision making
Definiens Tissue Studio surpasses every other image analysis application for translational research of
tissue-based biomarkers, delivering deeper insights, faster results and better decisions.

Definiens
Tissue Studio
delivers
DEEPER INSIGHTS by
Quantifing nuclear,
membrane and cytoplasmic
biomarkers on a cell-by-cell
basis
Supporting all standard
IHC tissue stains (blue, brown
and red), H&E and IF
Analyzing all slides and
TMA images from all major
image acquisition devices
FASTER RESULTS by
Identifing regions of
interest automatically
Operating through a simple,
intuitive user interface
Ofering unlimited
throughput with parallel
batch processing
BETTER DECISIONS by
Delivering extremely accurate
and reproducible results
Enabling multiparametric
investigations to identify
underlying correlations
Supporting translational
research through rapid
iterative investigation of
biomarkers
Multiparametric and morphological
features measured
IHC
No. of positive nuclei
No. of negative nuclei
Positive index
Cell and membrane features:
– Mean cell size (μm²)
– Mean IHC marker intensity (cell)
– Mean IHC marker intensity (cytoplasm)
– Mean IHC marker intensity (membrane)
– Mean ratio IHC marker intensity membrane
to cytoplasm
– Marker area (μm²)
No. and % of:
– Nuclei of low intensity
– Nuclei of medium intensity
– Nuclei of high intensity
No. and % of:
– Nuclei with small area
– Nuclei with medium area
– Nuclei with large area
No. and % of:
– Cells of negative intensity (0)
– Cells of low intensity (1+)
– Cells of medium intensity (2+)
– Cells of high intensity (3+)
No. and % of:
– Cells with small area
– Cells with medium area
– Cells with large area
H & E and IF
ROI area/marker area
Custom data export is also supported
Definiens Tissue Studio 5

System requirements
Defniens Tissue Studio is available with either two or four analysis engines to meet your
processing requirements.
TWO ANALYSIS ENGINES
Minimum:
• Windows XP Professional 64Bit SP2,
Vista-Business/Ultimate 64 Bit SP2
• Intel / AMD Quad Core 64 Bit 1.83Ghz +
• 8 Gb Memory
• 30Gb Disk Space
• 20“ 1600 x 1200 Display
• 100 MB Ethernet network connection
FOUR ANALYSIS ENGINES
Minimum:
• Windows XP Professional 64Bit SP2,
Vista-Business/Ultimate 64 Bit SP2
• Intel / AMD Dual CPU (Socket),
Quad Core 64 Bit 1.83Ghz +
• 12 Gb Memory
• 30Gb Disk Space
• 20“ 1600 x 1200 Display
• 100 MB Ethernet network connection
Recommended:
• Windows XP Professional -64Bit SP2,
Vista-Business/Ultimate 64 Bit SP2
• Intel Xeon/ AMD Quad Core 64 Bit 2.00Ghz+
• 12 Gb Memory (1.33GHz Bus)
• 30Gb Disk Space
• 24“ 1920 x 1200 Display +
• 1Gb Ethernet Connection
Recommended:
• Windows XP Professional 64Bit SP2,
Vista-Business/Ultimate 64 Bit SP2
• Intel Xeon/ AMD Dual CPU (Socket),
Quad Core 64 Bit 2.00Ghz+
• 16 Gb Memory (1.33GHz Bus)
• 30Gb Disk Space
• 24“ 1920 x 1200 Display +
• 1Gb Ethernet Connection
Definiens is the number one Enterprise Image Intelligence® company for analyzing and interpreting images
on every scale, from microscopic cell structures to satellite images.
The patented Definiens Cognition Network Technology®, developed by Nobel Laureate Prof. Gerd Binnig
and his team, emulates human cognitive processes of perception to extract intelligence from images.
If you are interested in learning more about how Definiens could address the challenges you face, please visit
our website.
www.definiens.com
Corporate Headquarters Americas Headquarters
Definiens AG Definiens Inc.
Trappentreustrasse 1 1719 State Route 10, Suite 118
80339 Munich Parsippany, New Jersey 07054
Germany USA
Tel. +49 (0)89 231180-0 Tel. +1-973-457-7150 DSL-0004-02-31080
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and Understanding Images are trademarks or registered trademarks of Definiens in the United States, the European Community, or certain other jurisdictions.
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