Spotted cDNA microarray image segmentation ... - IMT Bucharest

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260 S. Ni et al.Fig. 12. Histone genes under yeastalpha factor experiment.In the following we will focus on minute 70 of the experiment (when according to[7] the log ratio values should be negative) green in Figure 12.Figure 13 shows the ’Green Red Green Red’ cycle which was the same as inFigure 14. The ratio R g is equal to Y gX g. This ratio represents the DNA change (DNAexpression difference).X g denotes the g th spot pixel intensity of Cy3 channel (Green).Y g denotes the g th spot pixel intensity of Cy5 channel (Red). Logarithms of this ratiois log 2 R g . Using logarithmic transformation reduces the skewness of the distributionand improves the variance estimation. Log ratio of intensities from Red and Greenchannel was computed to represent histone genes expression in alpha factor using theACWE method.Fig. 13. Log ratio (R/G) of histone genes in alpha factor using ACWE method.In Figure 14 log ratio of intensities from Red and Green channel was computed torepresent histone genes expression in alpha factor using the SRG method. At minute70, it showed red. This is different from Figure 12.
Spotted cDNA microarray image segmentation using ACWE 261Fig. 14. Log ratio (R/G) of histone genes in alpha factor using SRG method.In Figure 15 the log ratio of intensities from Red and Green channel was computedto represent histone genes expression in alpha factor using the GOGAC method. Atminute 70, it showed red. This is different from Figure 12.Fig. 15. Log ratio (R/G) of histone genes in alpha factor using GOGAC method.Therefore, we can conclude that ACWE is better than SRG and GOGAC since itmatched the exact biological cell cycle.
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