Vacu-Blot - Biometra

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Note: Eliminate all air bubbles between the agarose gel, the membrane andthe rubber frame by rolling a glass pipette or a similar object across the gel.7. Place the cover plate with the buffer reservoir on the filter plate so that it corresponds to theorientation of the guide pins.8. Tighten both parts of the Vacu Blot by using the clips.9. Apply vacuum to the transfer unit before pouring transfer buffer into the upper reservoir.Otherwise your agarose gel might float up.10. Transfer time of nucleic acid fragments from an 0.8 % concentrated agarose gel to themembrane is about 1 h to 2 h (when applying a vacuum of 50 to 100 mbar below ambient).These values are only guideline for transferring nucleic acids from 0.5 kb - 10 kb.Cover plate with buffer reservoirAgarose gelRubber sheetMembraneWhatman 3MM filter paperPorous filter plateSupportClipTankStabilizerVacuum connectorFig. 1: Set up of a vacuum blotting sandwich7
8 Disassembling the unit1. First interrupt the vacuum before opening the clips. When using the vacuum pump delivered withthe system (Order-No.: 053-000/090/091) you can apply ambient pressure by opening the flowvalve.2. Remove the transfer buffer from the upper buffer reservoir. Afterwards the apparatus can bedisassembled in reverse order to assembling.3. The membrane can be further processed as usually, e.g. by UV-light crosslinking or by bakingat 80 o C.4. Please rinse all parts of the Vacu Blot after use with deionised water. Pay attention to the guidelines for cleaning given in chapter “4 Safety and Operating Informations”.8
Page 1 and 2: Vacu-Blot System (Order No. 053-000
Page 3 and 4: 1 Intended Uses And SpecificationsT
Page 5 and 6: 3 Ordering InformationItem:Order No
Page 7: 6 LocationPlace the chamber in prox
Page 13 and 14: Equipment Decontamination Certifica
Page 15: WarrantyThis laboratory instrument

Vacu-Blot - Biometra

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