Curriculum Vitae - NMR Spectroscopy Research Group

PERSONAL INFORMATION
Curriculum Vitae of Klaartje Houben, January 2011
Curriculum Vitae
Name: Klaartje Houben Address: Springweg 149
E-mail: klaartje.houben@gmail.com 3511 VN Utrecht
Date of birth: March 15, 1976 Phone: +31 (0)30 75 30 454
Nationality: Dutch +31 (0)6 38 27 22 47
Web-site: http://www.nmr.chem.uu.nl/~klaartje
WORK EXPERIENCE
October 1 st 2007 – today
Post-doc (VENI laureate), NMR research group / Membrane Enzymology, Chemistry Department,
Utrecht University: Combined solution and solid-state NMR study of yeast membrane proteins Hor7p
and Pmp3p.
- The goal of my Veni project is to unravel the structure of a family of small plant membrane
proteins in order to understand their role in salt-tolerance of these plants. For that I cloned several
of these proteins in different constructs, developed a protocol to express and purify them and
recorded many different NMR experiments. Currently I am in the process of structure calculations.
- I am responsible for the NMR teaching during the 3 rd year structural biology course.
November 2004 – September 2007
Post-doc, Laboratoire de RMN, Institut de Biologie Structurale, Grenoble, France: Dynamics and
interaction of two (partially) unstructured viral protein domains.
- I developed and applied NMR methods to study the intrinsic dynamics of unstructured proteins.
January 2000 – September 2004
Ph. D. student, Department of NMR Spectroscopy, Utrecht University (directors: Prof. Dr. R. Boelens
and Prof. Dr. R. Kaptein): Studies on protein dynamics. Development and application of NMR
relaxation methods. (Received degree on the 23 rd of September 2004)
- I developed a novel technique to study protein side-chain dynamics, described the dynamics of
several different proteins and proposed a functional role for these dynamics.
EDUCATIONAL QUALIFICATION
1994-1999 M. Sc. Chemistry, Utrecht University, Cum Laude, November 29 th 1999
Last year Research Project NMR Spectroscopy (1 year)
Undergraduate Report: “NMR study of CI2 and DSAI, a Serine Protease Inhibitor and a Serine
Protease”, supervisors: Dr. Giuseppe Melancini and Prof. Dr. Rolf Boelens
Literature Report: “Transverse Relaxation Optimized Spectroscopy”, supervisor: Prof. dr. Rolf
Boelens
Short Research Project Physical Organic Chemistry (3 months)
Undergraduate Report: “4,4’-bis(tetrahydro-4H-thiopyranylideen), a bridging ligand in a
pentaamine-ruthenium-complex”, supervisors: Dr. A Marsman and Prof. Dr. L. Jenneskens
1988-1994 Gymnasium, Dominicus College, Nijmegen
WORKSHOPS & COURSES
2008 NCCR-EMBN Practical course in 2D membrane protein crystallization and observation,
Basel, Switzerland (October 20-14)
2005 Varian Workshop “Hadamard NMR Spectroscopy”, Grenoble, France (May 4)
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Curriculum Vitae of Klaartje Houben, January 2011
2003 “Solid-state NMR: Basic principles and Biophysical Applications”, Marc Baldus, Utrecht
2002 Advanced Workshop “Dynamic Presentation” organised by Ludens Seminars and Private
Coaching, Utrecht, The Netherlands (1 week)
2001 International School of Structural Biology and Magnetic Resonance: 5 th course “Protein
Structure, Dynamics, Genomics and Function”, Erice (Sicily), Italy (June 5-15)
2000 BNRA course “From data to Structures”, Nijmegen-Utrecht (May/June 3 weeks)
EMBO course “Multidimensional NMR in Structural Biology”, Il Ciocco, Italy (August 13-
18)
1999 BNRA course “Advanced NMR Spectroscopy”, Nijmegen-Utrecht (May/June 3 weeks)
SCHOLARSHIPS and PRIZES
- Travel grant, ICMRBS (2010)
- Marie-Curie Fellowship covering expenses NCCR-EMBN workshop (2008)
- NWO ‘Veni’ young researchers fellowship (2007): 3 years research funding
- C.N.R.S. campagne de recrutement de post-doctorants (2006): 1 year post-doc
- EUROMAR conference grant (2005)
- Ministère délégué à la Recherche (2005): 1 year post-doc
- NWO-Talent Fellowship (2004): 1 year post-doc
- Student Travel Stipend of the 43 rd ENC (2002)
PUBLICATIONS
M.R. Jensen, P. Bernadó, K. Houben, L. Blanchard, D. Marion, R.W.H. Ruigrok & M. Blackledge,
Structural disorder within sendai virus nucleoprotein and phosphoprotein: insight into the structural
basis of molecular recognition (2010), Protein Pept Lett 17(8): 952-60
M.R. Jensen, K. Houben, E. Lesop, L. Blanchard, R.W.H. Ruigrok & M. Blackledge. Quantitative
Conformational Analysis of Partially Folded Proteins from Residual Dipolar Couplings: Application
to the Molecular Recognition Element of Sendai Virus Nucleoprotein (2008), JACS 130(25), 8055-61
K. Houben, L. Blanchard, M. Blackledge & D. Marion. Intrinsic dynamics of the partly folded PX
domain from the Sendai virus RNA polymerase co-factor P (2007), Biophysical J. 93(8), 2830-44
K. Houben, D. Marion, N. Tarbouriech, R.W.H. Ruigrok & L. Blanchard. INTERACTION OF THE C-
TERMINAL DOMAINS OF SENDAI VIRUS N AND P PROTEINS: Comparison of polymerase-nucleocapsid
interaction within the paramyxovirus family (2007), J. Virol. 81(13), 6807-16
N. Pannetier, K. Houben, L. Blanchard & D. Marion. Optimized 3D-NMR sampling for resonance
assignment of partially unfolded proteins (2007), J. Magn. Reson. 186 (1), 142-9
C. Bernard, K. Houben, N.M. Derix, D. Marks, M.A. van der Horst, K.J. Hellingwerf, R. Boelens, R.
Kaptein & N.A.J. van Nuland (2005) The solution structure of a transient photoreceptor intermediate:
Δ25 Photoactive Yellow Protein Structure 13 (7), 953-62
K. Houben, E. Wasielewski, C. Dominguez, E. Kellenberger, R.A. Atkinson, H.Th.M. Timmers, B.
Kieffer & R. Boelens (2005) Dynamics and metal exchange properties of C4C4 RING domains from
CNOT4 and the p44 subunit of TFIIH J. Mol. Biol. 349 (3), 621-37
K. Houben, C. Dominguez, F.M.A. van Schaik, H.Th.M. Timmers, A.M.J.J. Bonvin & R.Boelens
(2004) Solution structure of the ubiquitin-conjugating enzyme UbcH5B J. Mol. Biol. 344 (2), 513-526
K. Houben & R. Boelens (2004) Side Chain Dynamics monitored by 13 C- 13 C cross-relaxation J.
Biomol. NMR 29, 151-66
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Curriculum Vitae of Klaartje Houben, January 2011
A.M.J.J. Bonvin, K. Houben, M. Guenneugues, R. Kaptein & R. Boelens (2001) Rapid protein fold
determination using secondary chemical shifts and cross-hydrogen bond 15 N- 13 C’scalar couplings
( 3hb JNC’) J. Biomol. NMR 21, 221-33
INVITED LECTURES
2009: Preparation of membrane proteins for NMR: when the going gets tough, the tough gets going,
BC lecture, Biophysical Chemistry, Groningen (host: Dr. F.A.A. Mulder)
2005: Development and application of NMR relaxation methods to study protein structure and
dynamics, Institute of Molecular Biology and Biophysics, ETH Zürich, Switzerland (host: Prof. Dr. F.
Alain)
2004: I. Structure and dynamics of UbcH5B, II. Side chain dynamics using 13 C- 13 C cross-relaxation,
Laboratoire de RMN, IBS, Grenoble, France (host: Dr. D. Marion)
2002: 13 C- 13 C cross-relaxation as a tool for studying side chain dynamics, Biophysical Chemistry,
Lund University, Sweden (host: Prof. Dr. M. Akke)
CONFERENCES (including at least a poster presentation)
2010: NWO meeting Protein Research, Nucleic acids, Lipids & Biomembranes, Veldhoven (speaker)
2010: 32 nd Discussion Meeting and Joint Benelux/German Magnetic Resonance Conference, Münster,
Germany (speaker)
2010: International Conference on Magnetic Resonance in Biological Systems, Cairns, Australia
2009: Keystone conference on Frontiers of NMR in Biology, Santa Fe, New-Mexico, USA
2008: Lipid PRISM meeting, Frankfurt, Germany (speaker)
2008: 43 rd Dutch NMR-DG, Eindhoven, The Netherlands
2008: NWO meeting Protein Research, Nucleic acids, Lipids & Biomembranes, Veldhoven
2008: Bijvoet Anniversary Symposium, Utrecht, The Netherlands
2008: NMR a tool for Biology VIII, Institut Pasteur, Paris, France
2007: French-Japanese seminar on structural dynamics in proteins, Grenoble, France (speaker)
2007: EUROMAR Magnetic Resonance Conference, Taragona, Spain
2006: PSB Science day, Grenoble, France (speaker)
2006: Gordon Conference Computational Aspects of Biomolecular NMR, Aussois, France (speaker)
2006: NMR a tool for Biology VII, Institut Pasteur, Paris, France
2005: VIII ième Rencontre Rhône-Alpes de RMN, Grenoble, France (speaker)
2005: EUROMAR / European Experimental NMR Conference, Veldhoven, The Netherlands (speaker)
2004: NWO-CW meeting Ontwerp & Synthese, Structuur & Reactiviteit and Biomoleculaire Chemie,
Lunteren, The Netherlands (speaker)
2004: NMR a tool for Biology VI, Institut Pasteur, Paris, France
2003: Bijvoet Autumn School ‘Folding and Dynamics’, The Netherlands (speaker)
2003: 7 th European Large Scale Facility User Meeting, Oosterbeek, The Netherlands
2002: 43 rd Experimental NMR Conference, Asilomar, USA (speaker)
2002: 6 th European Large Scale Facility User Meeting, Montecatini, Italy
2002: XX th International Conference on Magnetic Resonance in Biological Systems, Toronto, Canada
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2002: NMR: a tool for Biology V, Institut Pasteur, Paris, France
2001: 5 th European Large Scale Facility User Meeting, Frankfurt, Germany
-
TEACHING - EXPERIENCE and QUALIFICATION
Curriculum Vitae of Klaartje Houben, January 2011
2010 - Lecturer at the Molecular and Cellular Life-Sciences introductory masters course:
Introduction solution NMR
- Lecturer at the Bijvoet summerschool ‘Exploring Nature’s Molecular Machines’,
Utrecht: Introduction solution NMR
- Supervisor Master’s literature thesis (Sabrina Berkamp) “Biophysicial methods
used to study intrinsically disordered proteins”
2009 - Lecturer in the graduate course ‘Advanced NMR spectroscopy’ Utrecht-Nijmegen
(June 4): Relaxation theory
- Supervisor Master’s Research Project (Margriet Hermans, 6 months) “Finding the
NMR based structure of Pmp3”
2008 Supervisor Bachelor Research Project (Margriet Hermans, 2 months) “Towards
NMR analysis of small membrane proteins: Over- expression and purification of
isotopically labeled yeast-Pmp3”
2007-now Lecturer in 3 rd year Chemistry Structural Biology course: Basics of NMR
2007 Supervisor Stage M2 (Nikolas Melcher, 3 months): “Étude d’échange avec l’eau
d’une protéine non-structurée par RMN”
2006 Qualification Maître de Conférence (Analytical Chemistry, Organic Chemsitry,
Biochemistry) in France
2005 Lecturer in the graduate course ‘Advanced NMR spectroscopy’ Utrecht-Nijmegen (6
& 7 june 2005): Protein dynamics characterized by NMR
2002-2003 Supervisor Last Year Research Project Undergraduate (Sanne Nabuurs, 9
months):“NMR Relaxation of CI2: Measurements & Analysis”
2000 - Assistant analytical chemistry 2 nd year practical course (150 hrs), Faculty of
Chemistry, Utrecht University
- Assistant analytical chemistry 1 st year practical course (100 hrs), Faculty of
Chemistry (Natuurwetenschappen), Utrecht University
1998 Assistant analytical chemistry 1 st year practical course (120 hrs), Faculty of
Pharmaceutical Sciences, Utrecht University
-
ORGANIZATIONAL -
ACTIVITIES
2009 Co-organizer Bijvoet tutorial symposium
2006 Organization of Ph.D. and post-doc meetings at the IBS Grenoble
2005+2006 Organization ‘Fête de la Science’ at the IBS Grenoble
2000-2003 Member of the board of the NMR department (in charge of teaching-duties)
2000-2002 Member of the Ph.D. student platform of the Bijvoet Center of Biomolecular Research
-
SKILLS -
Techniques: Multidimensional NMR spectroscopy, Biochemical and Molecular Biology techniques
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(cloning, expression and purification proteins)
Operating systems: Mac OS, Windows, Linux
Curriculum Vitae of Klaartje Houben, January 2011
Software: NMR related software packages (Bruker Biospin, Varian VnmrJ, NMRPipe, NMRView,
Sparky), Molecular structure viewers (Rasmol, Pymol, Molmol), Scripting language Awk, Microsoft
office, Apple iWork
Languages: Dutch (native), French (fluent), English (fluent), German (basic)
-
REFERENCES
Prof. dr. Rolf Boelens Prof. dr. Alexandre M.J.J Bonvin
Bijvoet Center for Biomolecular Research Bijvoet Center for Biomolecular Research
NMR spectroscopy research group NMR spectroscopy research group
Padualaan 8 Padualaan 8
3584 CH Utrecht 3584 CH Utrecht
The Netherlands The Netherlands
+31 (0)30 253 4035 +31 (0)30 253 3859
R.Boelens@uu.nl A.M.J.J.Bonvin@uu.nl
Prof. dr. Gerrit van Meer Dr. Joost C.M. Holthuis
Bijvoet Center for Biomolecular Research Bijvoet Center for Biomolecular Research
Membrane Enzymology Membrane Enzymology
Padualaan 8 Padualaan 8
3584 CH Utrecht 3584 CH Utrecht
The Netherlands The Netherlands
+31 (0)30 253 3427 +31 (0)30 253 6630
G.VanMeer@uu.nl J.C.Holthuis@uu.nl
Dr. Dominique Marion Dr. Martin Blackledge
Institut de Biologie Structurale Institut de Biologie Structurale
Laboratiore de RMN Laboratiore de RMN
41 rue Jules Horowitz 41 rue Jules Horowitz
38027 Grenoble 38027 Grenoble
France France
+33 (0)438 78 36 98 +33 (0)438 78 95 54
Dominique.Marion@ibs.fr Martin.Blackledge@ibs.fr
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